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SDS-PAGE and Western Blotting

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Topic	Stats	Last Post Info
Reference protein give different expression Started by tierarztkarakus, 17 Feb 2013 Western blot	3 replies 197 views	bob1 18 Feb 2013
Tricene gel Vs. Bis-tris gel Started by immunolion, 17 Feb 2013 tricene, stacking Electrophoresis	0 replies 178 views	immunolion 17 Feb 2013
Excess betamercaptoethanol during sample preparation Started by criscastells, 14 Feb 2013	4 replies 174 views	criscastells 15 Feb 2013
Recommendation for GOOD anti-6xHis-tag and anti-Strep-II-tag antibodies? Started by nanopore, 07 Feb 2013	5 replies 228 views	PhDinAcronyms 14 Feb 2013
Multiple Non-Specific Bands in Western Blot Detected... Started by singhabhilasha.84, 13 Feb 2013 non specific bands WB	1 reply 203 views	Curtis 13 Feb 2013
Far western blot: probe concentration Started by Bioscientist, 11 Feb 2013	1 reply 180 views	phage434 12 Feb 2013
HELP making 6X SDS Loading Buffer for SDS-PAGE Started by SPombe3, 04 Feb 2013 SDS buffer PAGE 6X SB	3 replies 293 views	mdfenko 08 Feb 2013
Low Protein Content In Cell Lysate Started by shrenik3004, 07 Feb 2013 RIPA	1 reply 228 views	Curtis 07 Feb 2013
B- actin band looks like dumbbells! Started by kiwidiwi, 30 Jan 2013	2 replies 229 views	kiwidiwi 04 Feb 2013
SDS PAGE gels Started by Bindu, 26 Nov 2012	5 replies 526 views	bob1 30 Jan 2013
Which antibody is good for HA-tag with low background bands? Started by memari, 18 Jan 2013 HA-tag Antibody, HA-tag	1 reply 218 views	jerryshelly1 30 Jan 2013
western blot explenation Started by lyok, 29 Jan 2013	8 replies 284 views	lyok 30 Jan 2013
Western blotting Started by micronagu, 22 Jan 2013 cell biology	9 replies 396 views	micronagu 23 Jan 2013
WB of cellular proteins in human serum Started by Dabronzo, 23 Jan 2013	2 replies 205 views	Dabronzo 23 Jan 2013
How long can I store cell lysis buffer at 4C for? Started by science noob, 20 Jan 2013	1 reply 248 views	bob1 20 Jan 2013
Blocking solution mistake, please help! Started by Fluffy, 15 Jan 2013	2 replies 207 views	Fluffy 16 Jan 2013
Acid urea gel electrophoresis (AUGE) Started by pakbiochemist, 04 Sep 2012	7 replies 323 views	yongzhi tian 16 Jan 2013
Wet transfer of proteins between 100-200kDa Started by LacquerHead, 11 Jan 2013	2 replies 253 views	bob1 14 Jan 2013
Running time is excessively long, bunched ladder Started by asta6801, 10 Dec 2012 western blot	2 replies 309 views	asta6801 13 Jan 2013
Membrane Protein SDS PAGE and WB Started by yalegenetics, 11 Jan 2013 Membrane Protein SDS PAG	1 reply 260 views	Pangea 12 Jan 2013
western blot and actin issue Started by silvepa23, 10 Jan 2013	2 replies 181 views	almost a doctor 11 Jan 2013
Low-temperature SDS-PAGE: tips and suggestions Started by yulia24, 08 Jan 2013	4 replies 189 views	yulia24 08 Jan 2013
conjugated antibodies in immunoprecipitation Started by philman, 04 Jan 2013	3 replies 262 views	philman 07 Jan 2013
Interpretation & Troubleshooting on SDS/Native PAGE Started by schroddycat, 15 Dec 2012 sds, page, native	5 replies 649 views	mdfenko 27 Dec 2012
Separating proteing with difference of 5Kd on western blot Started by Preeti Dave, 18 Dec 2012 Western Blot	4 replies 355 views	bob1 20 Dec 2012