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SDS-PAGE and Western Blotting

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Topic	Stats	Last Post Info
Help with high background (have tried most trouble shooting) Started by crom80, 21 Sep 2012	8 replies 384 views	crom80 25 Oct 2012
Laemmli buffer a bit green or yellow after boiling!!?!? Started by Tai, 24 Oct 2012 poor image	3 replies 253 views	bob1 24 Oct 2012
How to load equal amount of protein without using spectramax Started by junbug, 18 Oct 2012	4 replies 255 views	bob1 19 Oct 2012
Western blot membrane imaging Started by junbug, 17 Oct 2012	7 replies 251 views	junbug 19 Oct 2012
Detection of T7 RNA polymerase by WB Started by pDNA, 17 Oct 2012	6 replies 255 views	pDNA 18 Oct 2012
Please help with odd bands and smears on Western blot Started by argonarin, 18 Oct 2012 western blot, electrophoresis and 1 more...	4 replies 383 views	science noob 18 Oct 2012
TNF alpha and IL 1b Western Blot Started by Citron, 15 Sep 2012 17 Kda protein	1 reply 248 views	zodiac1505 18 Oct 2012
Shift in the position of band onto the western blot Started by chimera, 16 Oct 2012	3 replies 182 views	bob1 17 Oct 2012
Ghost and inverse bands and high background. Help! Started by Elioelio, 11 Oct 2012 ghost band, high background and 3 more...	3 replies 285 views	Elioelio 15 Oct 2012
confusing Western results Started by crisalis82, 03 Oct 2012 Western Blot, electrophoresis	5 replies 465 views	zienpiggie 13 Oct 2012
5% BSA blocking buffer Started by moljul, 10 Oct 2012 long time storage?	3 replies 319 views	cbf88 12 Oct 2012
Storage of Membrane before addition of primary antibody Started by 2xzwei, 08 Oct 2012	3 replies 216 views	cbf88 12 Oct 2012
"Mickey Mouse" bands Started by LAH603, 05 Oct 2012 Western Blot	5 replies 392 views	bob1 09 Oct 2012
TAU gel electrophoresis of histones Started by pakbiochemist, 01 Oct 2012	4 replies 223 views	mdfenko 05 Oct 2012
aditional band on WB Started by myself, 03 Oct 2012 WB	1 reply 271 views	bob1 04 Oct 2012
Transferring >150 kDA proteins conditions Started by pinkc, 03 Oct 2012	3 replies 216 views	bob1 04 Oct 2012
SDS-PAGE : when the transfer stops Started by Tim Elliott, 03 Oct 2012	1 reply 191 views	bob1 04 Oct 2012
Does anyone have a reoccuring problem executing Gel electrophoresis? Started by MoezatTokue, 03 Oct 2012 Buffers, SDS, electrophoresis and 2 more...	1 reply 328 views	bob1 03 Oct 2012
Bands dark on sides and light in middle Started by Caribou, 27 Sep 2012	7 replies 330 views	jmiller623 03 Oct 2012
Acrylamid Started by Izanloo, 10 Sep 2012	5 replies 273 views	MoezatTokue 03 Oct 2012
question about protein expression normalization. Started by R.Mufti, 26 Sep 2012 Normalization	6 replies 217 views	cbf88 03 Oct 2012
Inclusion bodies in yeast Started by Scienceboy, 02 Oct 2012	0 replies 130 views	Scienceboy 02 Oct 2012
How to prepare blood serum for western blot? Started by Tai, 19 Sep 2012	3 replies 208 views	Tai 01 Oct 2012
SDS-PAGE trouble with protein concentration Started by Sweeps, 11 Sep 2012	2 replies 271 views	cbf88 29 Sep 2012
TCA Precipitation to remove albumin Started by Anupa, 18 Aug 2012 TCA Precipitation, albumin	6 replies 298 views	cbf88 29 Sep 2012