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SDS-PAGE and Western Blotting

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Topic	Stats	Last Post Info
Pinned SDS-PAGE: casting Started by Doan Lan, 02 Jun 2002 polymerization, TEMED, APS 1 2	Hot 26 replies 14,897 views	shivangipurohit 27 Jun 2012
Pinned Cutting membrane after transfer Started by Pipette Dude, 01 Apr 2009 1 2	Hot 28 replies 14,637 views	mdfenko 07 May 2012
Pinned Western Blotting background troubleshooting Started by raje, 22 Jun 2004 1 2	Hot 22 replies 14,059 views	js86 29 Jun 2011
Pinned Tips on re-use of primary antibody in Western blot Started by jiro_killua, 08 Mar 2009	Hot 14 replies 12,057 views	ajxutao7 10 May 2011
Pinned x-ray films vs direct imaging Started by almost a doctor, 18 Jun 2009 1 2	Hot 17 replies 10,229 views	Inmost sun 19 Feb 2011
Pinned Western Blotting- A beginner Guide Started by Minnie Mouse, 01 Feb 2009	3 replies 8,840 views	GNANA 17 Sep 2010
Pinned semi-dry western conditions Started by flashboy, 25 Mar 2004	Hot 14 replies 10,713 views	kottila 04 Aug 2010
Pinned PVDF vs. Nitrocellulose for Western blot Started by Mr Jefferson, 31 Jan 2009 1 2	Hot 20 replies 31,073 views	mdfenko 04 Jun 2010
Pinned Old threads on SDS-PAGE and Western Blotting are achived here Started by bioforum, 03 Feb 2009	0 replies 5,058 views	bioforum 03 Feb 2009
Western Blot - Quantifying Tumor samples from two different blots Started by youngscientist123456, Yesterday, 03:42 PM	1 reply 30 views	bob1 Yesterday, 05:41 PM
What is different between Native protein and Denatured protein samples. Started by Tai, 08 May 2013 western blot	4 replies 132 views	mdfenko 15 May 2013
Protocol for detecting soluble, secreted proteins on a Western? Started by JYaron, 13 May 2013 il-1b, western blot, caspase-1	1 reply 71 views	mdfenko 14 May 2013
Protein amount to load for publication figure? Started by HOYAJM, 10 May 2013	4 replies 106 views	HOYAJM 11 May 2013
3 extra bands show up on my western after an IP Started by DrZeus, 07 May 2013 ip, igg, heavy, western, mouse	2 replies 76 views	bob1 08 May 2013
Native gel electrophoresis: Can it tell if a protein is forming dimer? Started by Bioscientist, 08 May 2013	1 reply 68 views	bob1 08 May 2013
IgG heavy chain from IP Started by DrZeus, 05 May 2013 IP, rabbit, IgG, antibody, heavy	1 reply 140 views	bob1 05 May 2013
Uneven beta actin Started by asta6801, 15 Apr 2013 western blot, methods	2 replies 221 views	asta6801 30 Apr 2013
Western Blotting loading control for large proteins Started by alchemic, 30 Apr 2013	2 replies 125 views	alchemic 30 Apr 2013
Blue Native Gel - denaturing proteins after transfer? Started by Bobby007, 07 Aug 2009	9 replies 3,004 views	mdfenko 22 Apr 2013
Western blot antibody Started by weilun, 18 Apr 2013	0 replies 170 views	weilun 18 Apr 2013
NCOR1 western Started by abrahas, 09 Apr 2013	8 replies 271 views	mdfenko 15 Apr 2013
Minimum volume antibody solution for Western blot in tray? Started by assembler01, 09 Apr 2013 antibody, volume, western blot	4 replies 245 views	bob1 09 Apr 2013
The Good Antibodies for Western Blots Thread Started by thebigpiro, 21 Mar 2011	3 replies 799 views	Tom M 09 Apr 2013
Discrepancy between WB and Flow cytometry result Started by Tabaluga, 08 Apr 2013 Flow cytometry, Western Blot and 2 more...	5 replies 317 views	Tabaluga 08 Apr 2013
Do cell signaling antibodies work with 1h RT incubation? Started by Iceage, 06 Apr 2013	2 replies 197 views	PhDinAcronyms 07 Apr 2013