SDS-PAGE and Western Blotting

PVDF vs. Nitrocellulose for Western blot

• 1
• 2

SDS-PAGE: casting

Started by Doan Lan, 02 Jun 2002   polymerization, TEMED, APS

• 1
• 2

Cutting membrane after transfer

• 1
• 2

Western Blotting background troubleshooting

• 1
• 2

Tips on re-use of primary antibody in Western blot

x-ray films vs direct imaging

• 1
• 2

semi-dry western conditions

Western Blotting- A beginner Guide

Old threads on SDS-PAGE and Western Blotting are achived here

Excess acrylamide in wells

• 1
• 2
• 3

Multiple antibody blotting - western blot

• 1
• 2

Visualizing Small Peptides

• 1
• 2

Why do I have to almost "burn" my transfer before I can detect??

• 1
• 2

ImageJ analysis on IFs

Protein extraction from cell culture media

Loading control for nuclear extracts

Problem with "smiling effect" on SDS-PAGE gel

western blot

• 1
• 2

Protein much larger on Western blot than predicted

How to extract protein from SDS-PAGE gel?

smear bands in western blot

lysate buffer

why BOIL.. samples for sdspage

Problem with running SDS page gel

Protein size and gel percentage