SDS-PAGE and Western Blotting

PVDF vs. Nitrocellulose for Western blot

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SDS-PAGE: casting

Started by Doan Lan, 02 Jun 2002   polymerization, TEMED, APS

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Cutting membrane after transfer

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Western Blotting background troubleshooting

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Tips on re-use of primary antibody in Western blot

x-ray films vs direct imaging

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semi-dry western conditions

Western Blotting- A beginner Guide

Old threads on SDS-PAGE and Western Blotting are achived here

Excess acrylamide in wells

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Multiple antibody blotting - western blot

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Visualizing Small Peptides

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ImageJ analysis on IFs

Why do I have to almost "burn" my transfer before I can detect??

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Protein extraction from cell culture media

Loading control for nuclear extracts

Problem with "smiling effect" on SDS-PAGE gel

western blot

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Protein much larger on Western blot than predicted

How to extract protein from SDS-PAGE gel?

lysate buffer

smear bands in western blot

why BOIL.. samples for sdspage

protein disapeared from gel!

Problem with running SDS page gel