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SDS-PAGE and Western Blotting

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Topic	Stats	Last Post Info
Pinned SDS-PAGE: casting Started by Doan Lan, 02 Jun 2002 polymerization, TEMED, APS 1 2	Hot 26 replies 27,534 views	shivangipurohit 27 Jun 2012
Pinned Cutting membrane after transfer Started by Pipette Dude, 01 Apr 2009 1 2	Hot 27 replies 25,797 views	mdfenko 07 May 2012
Pinned Western Blotting background troubleshooting Started by raje, 22 Jun 2004 1 2	Hot 22 replies 25,710 views	js86 29 Jun 2011
Pinned Tips on re-use of primary antibody in Western blot Started by jiro_killua, 08 Mar 2009	Hot 14 replies 21,296 views	ajxutao7 10 May 2011
Pinned x-ray films vs direct imaging Started by almost a doctor, 18 Jun 2009 1 2	Hot 17 replies 20,742 views	Inmost sun 19 Feb 2011
Pinned Western Blotting- A beginner Guide Started by Minnie Mouse, 01 Feb 2009	3 replies 18,028 views	GNANA 17 Sep 2010
Pinned semi-dry western conditions Started by flashboy, 25 Mar 2004	Hot 14 replies 19,372 views	kottila 04 Aug 2010
Pinned PVDF vs. Nitrocellulose for Western blot Started by Mr Jefferson, 31 Jan 2009 1 2	Hot 20 replies 45,599 views	mdfenko 04 Jun 2010
Pinned Old threads on SDS-PAGE and Western Blotting are achived here Started by bioforum, 03 Feb 2009	0 replies 12,957 views	bioforum 03 Feb 2009
Running Novex type precasts on BioRad Mini Protean Started by Trof, 09 Sep 2019	2 replies 128 views	Trof 13 Sep 2019
double bands on p-p70s6k WB Started by ylu, 06 Sep 2019 western blotting	3 replies 193 views	ylu 09 Sep 2019
Answered 10% SDS Adjusting pH??? Started by Vui, 19 Nov 2018 SDS, pH, Adjusting	10 replies 1,251 views	bob1 01 Sep 2019
SDS PAGE bands Started by LinaAg, 26 Apr 2019	2 replies 927 views	LinaAg 02 May 2019
why Tris-glycine buffer divided by w/ SDS and w/o SDS? Started by koreanraichu, 20 Jan 2019	1 reply 860 views	bob1 20 Jan 2019
Can anyone help me with Western blotting problem? Started by Vui, 06 Dec 2018 weaternblot, problem, IL36, Hacat	6 replies 1,110 views	Vui 09 Dec 2018
Answered western blotting troubleshooting Started by Vui, 08 Nov 2018 westernblot, smallprotein and 1 more...	3 replies 554 views	Vui 12 Nov 2018
Question on western blot picture Started by pito, 04 Sep 2018	2 replies 830 views	pito 06 Sep 2018
Lower band detected after alanine mutation Started by Thomson, 27 Aug 2018 western blot, protein and 2 more...	3 replies 2,086 views	Thomson 29 Aug 2018
Western-No bands for sample protein but loading control works Started by HawkeyeGrad, 12 Jul 2018 western blotprotein and 1 more...	5 replies 822 views	Artemis2007 13 Jul 2018
SDS-PAGE/2D gel and western blot for multiple proteins for multiple sample Started by Faisal SHAH, 05 Jul 2018 Electrophoresis and 2 more...	1 reply 758 views	mdfenko 06 Jul 2018
Severe or entire Blur formation in my SDS PAGE. Started by Siniya, 17 Apr 2018	1 reply 906 views	aimikins 17 Apr 2018
Severe or entire Blur formation in my SDS PAGE. Started by Siniya, 17 Apr 2018	0 replies 534 views	Siniya 17 Apr 2018
Standardization of SDS-PAGE!!! Started by Siniya, 04 Apr 2018	2 replies 915 views	mdfenko 05 Apr 2018
Sample buffer Started by Mad Researcher, 05 Feb 2018 sample buffer, DTT, Western Blot and 1 more...	1 reply 1,321 views	bob1 07 Feb 2018
How to prep mouse urine samples for SDS-PAGE and Coomassie staining? Started by esbena, 01 Dec 2017	2 replies 1,234 views	bob1 01 Dec 2017