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SDS-PAGE and Western Blotting

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Topic	Stats	Last Post Info
Pinned SDS-PAGE: casting Started by Doan Lan, 02 Jun 2002 polymerization, TEMED, APS 1 2	Hot 26 replies 25,420 views	shivangipurohit 27 Jun 2012
Pinned Cutting membrane after transfer Started by Pipette Dude, 01 Apr 2009 1 2	Hot 27 replies 23,783 views	mdfenko 07 May 2012
Pinned Western Blotting background troubleshooting Started by raje, 22 Jun 2004 1 2	Hot 22 replies 23,602 views	js86 29 Jun 2011
Pinned Tips on re-use of primary antibody in Western blot Started by jiro_killua, 08 Mar 2009	Hot 14 replies 19,530 views	ajxutao7 10 May 2011
Pinned x-ray films vs direct imaging Started by almost a doctor, 18 Jun 2009 1 2	Hot 17 replies 18,746 views	Inmost sun 19 Feb 2011
Pinned Western Blotting- A beginner Guide Started by Minnie Mouse, 01 Feb 2009	3 replies 16,316 views	GNANA 17 Sep 2010
Pinned semi-dry western conditions Started by flashboy, 25 Mar 2004	Hot 14 replies 17,659 views	kottila 04 Aug 2010
Pinned PVDF vs. Nitrocellulose for Western blot Started by Mr Jefferson, 31 Jan 2009 1 2	Hot 20 replies 43,392 views	mdfenko 04 Jun 2010
Pinned Old threads on SDS-PAGE and Western Blotting are achived here Started by bioforum, 03 Feb 2009	0 replies 11,359 views	bioforum 03 Feb 2009
Western-No bands for sample protein but loading control works Started by HawkeyeGrad, 12 Jul 2018 western blotprotein and 1 more...	5 replies 279 views	Artemis2007 13 Jul 2018
SDS-PAGE/2D gel and western blot for multiple proteins for multiple sample Started by Faisal SHAH, 05 Jul 2018 Electrophoresis and 2 more...	1 reply 284 views	mdfenko 06 Jul 2018
Severe or entire Blur formation in my SDS PAGE. Started by Siniya, 17 Apr 2018	1 reply 513 views	aimikins 17 Apr 2018
Severe or entire Blur formation in my SDS PAGE. Started by Siniya, 17 Apr 2018	0 replies 201 views	Siniya 17 Apr 2018
Standardization of SDS-PAGE!!! Started by Siniya, 04 Apr 2018	2 replies 396 views	mdfenko 05 Apr 2018
Sample buffer Started by Mad Researcher, 05 Feb 2018 sample buffer, DTT, Western Blot and 1 more...	1 reply 798 views	bob1 07 Feb 2018
How to prep mouse urine samples for SDS-PAGE and Coomassie staining? Started by esbena, 01 Dec 2017	2 replies 764 views	bob1 01 Dec 2017
WB imaging on Vilber-Lourmat Fusion Started by Trof, 25 Nov 2017	1 reply 551 views	bob1 28 Nov 2017
Forgot to block after stripping - please send help Started by NeuroG2019, 15 Nov 2017 stripping; western blot	1 reply 403 views	mdfenko 16 Nov 2017
Triton-X-100 Started by samita, 26 Oct 2017	1 reply 598 views	mdfenko 27 Oct 2017
How to add prepare protein sample for loading into the gel? Started by nasif, 05 Oct 2017 sds-page, western blot and 3 more...	3 replies 1,241 views	mdfenko 06 Oct 2017
Need help with my SDS-PAGE gels Started by Doraid, 22 Sep 2017	9 replies 790 views	mdfenko 27 Sep 2017
Transfer failure Started by rkay447, 14 Aug 2017	1 reply 897 views	bob1 15 Aug 2017
Couldn't detect Vimentin in western blot, in spite of positive result in IF Started by dvimal27, 17 Jul 2017 EMT, vimentin, western blot and 2 more...	0 replies 1,686 views	dvimal27 17 Jul 2017
Low weight protein degradation during western blot Started by kaaba, 16 May 2017 western blot, protein degradation and 3 more...	1 reply 1,515 views	mdfenko 16 May 2017
white light conversion screen alternative Started by ozgurtbt, 12 Apr 2017 comassie protein sdspage	1 reply 880 views	bob1 13 Apr 2017