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SDS-PAGE and Western Blotting

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Topic	Stats	Last Post Info
Pinned SDS-PAGE: casting Started by Doan Lan, 02 Jun 2002 polymerization, TEMED, APS 1 2	Hot 26 replies 17,680 views	shivangipurohit 27 Jun 2012
Pinned Cutting membrane after transfer Started by Pipette Dude, 01 Apr 2009 1 2	Hot 27 replies 16,440 views	mdfenko 07 May 2012
Pinned Western Blotting background troubleshooting Started by raje, 22 Jun 2004 1 2	Hot 22 replies 15,910 views	js86 29 Jun 2011
Pinned Tips on re-use of primary antibody in Western blot Started by jiro_killua, 08 Mar 2009	Hot 14 replies 13,404 views	ajxutao7 10 May 2011
Pinned x-ray films vs direct imaging Started by almost a doctor, 18 Jun 2009 1 2	Hot 17 replies 11,763 views	Inmost sun 19 Feb 2011
Pinned Western Blotting- A beginner Guide Started by Minnie Mouse, 01 Feb 2009	3 replies 10,064 views	GNANA 17 Sep 2010
Pinned semi-dry western conditions Started by flashboy, 25 Mar 2004	Hot 14 replies 11,604 views	kottila 04 Aug 2010
Pinned PVDF vs. Nitrocellulose for Western blot Started by Mr Jefferson, 31 Jan 2009 1 2	Hot 20 replies 35,264 views	mdfenko 04 Jun 2010
Pinned Old threads on SDS-PAGE and Western Blotting are achived here Started by bioforum, 03 Feb 2009	0 replies 5,757 views	bioforum 03 Feb 2009
Problem with Western Blotting - BCA assay shows protein but no protein bands whe Started by GraduateStudent2015, 03 Mar 2015 Western Blotting, Protein and 3 more...	3 replies 69 views	mdfenko Yesterday, 05:28 AM
Question on loss of signal between samples in western blot Started by Gradi, 26 Feb 2015 Western Blot, SDS PAGE, Proteins	4 replies 215 views	Gradi 27 Feb 2015
IP heavy chains not in Ponceau, but in final blot exposure Started by Hic5Prostate, 21 Feb 2015	1 reply 110 views	bob1 22 Feb 2015
Antibody-Biotinylation, detection via SDS-Gel Started by petertob, 02 Feb 2015 Antibody biotinylation and 4 more...	11 replies 534 views	mdfenko 18 Feb 2015
Some bands of similar intensity aren't seen in short exposure but are seen i Started by leighann36, 15 Feb 2015 western blot and 2 more...	1 reply 292 views	mdfenko 15 Feb 2015
Antibody Issues Started by Tayzz, 11 Feb 2015 Antibodies, HuH-7, Protein S and 1 more...	6 replies 199 views	mdfenko 12 Feb 2015
Why Glycomacropeptide (GMP) does not bind Coomassie Blue stain? Started by trungnghiatn, 05 Feb 2015	2 replies 136 views	trungnghiatn 05 Feb 2015
SDS page Started by Darlidada, 12 Jan 2015 western, gfp, antibody	8 replies 289 views	CPRES 30 Jan 2015
Is it possible to "over-block" a membrane? Started by science noob, 28 Jan 2015	5 replies 158 views	CPRES 30 Jan 2015
Troubleshooting for BN PAGE Started by neuron, 27 Jan 2015	4 replies 138 views	neuron 30 Jan 2015
Unspecific Western Signal from Protein A tag? Started by michael_p, 29 Jan 2015 Western Blotting	0 replies 408 views	michael_p 29 Jan 2015
SDS PAGE Destaining Started by Mag 22388, 21 Jan 2015 Destain	7 replies 327 views	mdfenko 26 Jan 2015
Answered Layer of water above separating gel mixes into gel Started by science noob, 19 Jan 2015	2 replies 184 views	CPRES 20 Jan 2015
A question about Gelanalyzer Started by trungnghiatn, 08 Jan 2015	8 replies 432 views	mdfenko 16 Jan 2015
PCR & Western Blot sample preperation Started by Homeostasis, 14 Jan 2015 Western blotting, PCR, analysis	2 replies 356 views	Homeostasis 15 Jan 2015
band obove the ladder Started by Perindu Bulan, 17 Dec 2014 electrophoresis, page	3 replies 461 views	CPRES 15 Jan 2015