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SDS-PAGE and Western Blotting

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Topic	Stats	Last Post Info
Pinned SDS-PAGE: casting Started by Doan Lan, 02 Jun 2002 polymerization, TEMED, APS 1 2	Hot 26 replies 16,032 views	shivangipurohit 27 Jun 2012
Pinned Cutting membrane after transfer Started by Pipette Dude, 01 Apr 2009 1 2	Hot 27 replies 15,573 views	mdfenko 07 May 2012
Pinned Western Blotting background troubleshooting Started by raje, 22 Jun 2004 1 2	Hot 22 replies 14,989 views	js86 29 Jun 2011
Pinned Tips on re-use of primary antibody in Western blot Started by jiro_killua, 08 Mar 2009	Hot 14 replies 12,744 views	ajxutao7 10 May 2011
Pinned x-ray films vs direct imaging Started by almost a doctor, 18 Jun 2009 1 2	Hot 17 replies 11,056 views	Inmost sun 19 Feb 2011
Pinned Western Blotting- A beginner Guide Started by Minnie Mouse, 01 Feb 2009	3 replies 9,480 views	GNANA 17 Sep 2010
Pinned semi-dry western conditions Started by flashboy, 25 Mar 2004	Hot 14 replies 11,167 views	kottila 04 Aug 2010
Pinned PVDF vs. Nitrocellulose for Western blot Started by Mr Jefferson, 31 Jan 2009 1 2	Hot 20 replies 33,856 views	mdfenko 04 Jun 2010
Pinned Old threads on SDS-PAGE and Western Blotting are achived here Started by bioforum, 03 Feb 2009	0 replies 5,413 views	bioforum 03 Feb 2009
Problems with wells on SDS PAGE gels Started by sir octopus, Yesterday, 10:17 AM	2 replies 53 views	mdfenko Today, 04:19 AM
high background with acetyl-lysine antibodies for IP immunoblot Started by crom80, 07 Feb 2014	1 reply 135 views	lilin_hust 09 Mar 2014
Protein is present, but not detected by antibody Started by fomalhaut, 03 Mar 2014 western blot, antibody and 1 more...	3 replies 123 views	fomalhaut 04 Mar 2014
Answered Are certain tissues prone to uneven loading? Issues with intestine. Started by phrustrated, 25 Feb 2014 Intestine, liver, muscle	6 replies 134 views	phrustrated 26 Feb 2014
secondary antibody creation Started by luckyjade, 25 Feb 2014	1 reply 75 views	mdfenko 26 Feb 2014
loading control issue in blot with H9c2 cell protein, pleeeeeasee help Started by niveda, 24 Feb 2014	2 replies 74 views	NingLiu 25 Feb 2014
Incomplete Blotting and staining of NC membrane Started by TZ89, 25 Jan 2014 Western Blot, ELISA, Blotting and 2 more...	7 replies 230 views	TZ89 25 Feb 2014
Low signal, weird background stains on blot Started by alphawhiskey, 04 Feb 2014	5 replies 207 views	alphawhiskey 10 Feb 2014
Chemiluminescence vs. Fluorescence visualisation of western blot Started by science noob, 26 Jan 2014	1 reply 139 views	bob1 26 Jan 2014
identification of major protein; public 2D/mass-spec databases? Started by comp3v, 19 Jan 2014	2 replies 175 views	comp3v 19 Jan 2014
having problems with p-erk antibody and other phosphoproteins antibodies Started by rb213, 17 Jan 2014	1 reply 752 views	mdfenko 18 Jan 2014
Help, protein ladder didn`t separate Started by Seamus04, 14 Jan 2014	3 replies 170 views	mdfenko 15 Jan 2014
Determining phosphorylation status of a protein by transfection Started by Shamoon Saljuqi, 07 Jan 2014	5 replies 197 views	bob1 08 Jan 2014
Will 10 min boiling disrupt BS3 cross linking? Started by Sander, 22 Dec 2013 Immunoprecipitation	7 replies 286 views	neuron 26 Dec 2013
Problem with Denative lysis Started by thanhtam, 15 Dec 2013	1 reply 238 views	mdfenko 16 Dec 2013
Effect of MeOH and acetic acid on primary-secondary complex Started by Josip Madunic, 13 Dec 2013	3 replies 227 views	Josip Madunic 16 Dec 2013