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SDS-PAGE and Western Blotting

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Topic	Stats	Last Post Info
Pinned SDS-PAGE: casting Started by Doan Lan, 02 Jun 2002 polymerization, TEMED, APS 1 2	Hot 26 replies 16,182 views	shivangipurohit 27 Jun 2012
Pinned Cutting membrane after transfer Started by Pipette Dude, 01 Apr 2009 1 2	Hot 27 replies 15,674 views	mdfenko 07 May 2012
Pinned Western Blotting background troubleshooting Started by raje, 22 Jun 2004 1 2	Hot 22 replies 15,101 views	js86 29 Jun 2011
Pinned Tips on re-use of primary antibody in Western blot Started by jiro_killua, 08 Mar 2009	Hot 14 replies 12,819 views	ajxutao7 10 May 2011
Pinned x-ray films vs direct imaging Started by almost a doctor, 18 Jun 2009 1 2	Hot 17 replies 11,138 views	Inmost sun 19 Feb 2011
Pinned Western Blotting- A beginner Guide Started by Minnie Mouse, 01 Feb 2009	3 replies 9,543 views	GNANA 17 Sep 2010
Pinned semi-dry western conditions Started by flashboy, 25 Mar 2004	Hot 14 replies 11,206 views	kottila 04 Aug 2010
Pinned PVDF vs. Nitrocellulose for Western blot Started by Mr Jefferson, 31 Jan 2009 1 2	Hot 20 replies 34,056 views	mdfenko 04 Jun 2010
Pinned Old threads on SDS-PAGE and Western Blotting are achived here Started by bioforum, 03 Feb 2009	0 replies 5,444 views	bioforum 03 Feb 2009
IgG heavy chain from IP Started by DrZeus, 05 May 2013 IP, rabbit, IgG, antibody, heavy	3 replies 774 views	bob1 17 Apr 2014
ECL Substrate Choices Started by Missle, 16 Apr 2014 ECL, Chemiluminescence and 1 more...	2 replies 70 views	memari 17 Apr 2014
Why does my gel have streaks instead of bands? Started by spatel21, 14 Apr 2014	1 reply 167 views	mdfenko 15 Apr 2014
Left ECL kit at room temp. Is it ruined? Started by sir octopus, 02 Apr 2014	1 reply 97 views	bob1 02 Apr 2014
what do you think is the problem with this polyacrylamide gel? Started by labrador422, 31 Mar 2014	2 replies 151 views	mdfenko 01 Apr 2014
Native PAGE problem - not entering gel enough Started by Missle, 27 Mar 2014 native page, resolution	5 replies 101 views	mdfenko 27 Mar 2014
Problems with wells on SDS PAGE gels Started by sir octopus, 11 Mar 2014	3 replies 195 views	sir octopus 25 Mar 2014
film saturation in immunoblots Started by Tom M, 13 Mar 2014	1 reply 105 views	bob1 13 Mar 2014
high background with acetyl-lysine antibodies for IP immunoblot Started by crom80, 07 Feb 2014	1 reply 219 views	lilin_hust 09 Mar 2014
Protein is present, but not detected by antibody Started by fomalhaut, 03 Mar 2014 western blot, antibody and 1 more...	3 replies 204 views	fomalhaut 04 Mar 2014
Answered Are certain tissues prone to uneven loading? Issues with intestine. Started by phrustrated, 25 Feb 2014 Intestine, liver, muscle	6 replies 229 views	phrustrated 26 Feb 2014
secondary antibody creation Started by luckyjade, 25 Feb 2014	1 reply 122 views	mdfenko 26 Feb 2014
loading control issue in blot with H9c2 cell protein, pleeeeeasee help Started by niveda, 24 Feb 2014	2 replies 128 views	NingLiu 25 Feb 2014
Incomplete Blotting and staining of NC membrane Started by TZ89, 25 Jan 2014 Western Blot, ELISA, Blotting and 2 more...	7 replies 294 views	TZ89 25 Feb 2014
Low signal, weird background stains on blot Started by alphawhiskey, 04 Feb 2014	5 replies 275 views	alphawhiskey 10 Feb 2014
Chemiluminescence vs. Fluorescence visualisation of western blot Started by science noob, 26 Jan 2014	1 reply 178 views	bob1 26 Jan 2014