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SDS-PAGE and Western Blotting

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Topic	Stats	Last Post Info
Pinned SDS-PAGE: casting Started by Doan Lan, 02 Jun 2002 polymerization, TEMED, APS 1 2	Hot 26 replies 27,012 views	shivangipurohit 27 Jun 2012
Pinned Cutting membrane after transfer Started by Pipette Dude, 01 Apr 2009 1 2	Hot 27 replies 25,284 views	mdfenko 07 May 2012
Pinned Western Blotting background troubleshooting Started by raje, 22 Jun 2004 1 2	Hot 22 replies 25,161 views	js86 29 Jun 2011
Pinned Tips on re-use of primary antibody in Western blot Started by jiro_killua, 08 Mar 2009	Hot 14 replies 20,844 views	ajxutao7 10 May 2011
Pinned x-ray films vs direct imaging Started by almost a doctor, 18 Jun 2009 1 2	Hot 17 replies 20,228 views	Inmost sun 19 Feb 2011
Pinned Western Blotting- A beginner Guide Started by Minnie Mouse, 01 Feb 2009	3 replies 17,581 views	GNANA 17 Sep 2010
Pinned semi-dry western conditions Started by flashboy, 25 Mar 2004	Hot 14 replies 18,933 views	kottila 04 Aug 2010
Pinned PVDF vs. Nitrocellulose for Western blot Started by Mr Jefferson, 31 Jan 2009 1 2	Hot 20 replies 45,035 views	mdfenko 04 Jun 2010
Pinned Old threads on SDS-PAGE and Western Blotting are achived here Started by bioforum, 03 Feb 2009	0 replies 12,532 views	bioforum 03 Feb 2009
SDS PAGE bands Started by LinaAg, 26 Apr 2019	2 replies 379 views	LinaAg 02 May 2019
why Tris-glycine buffer divided by w/ SDS and w/o SDS? Started by koreanraichu, 20 Jan 2019	1 reply 761 views	bob1 20 Jan 2019
Can anyone help me with Western blotting problem? Started by Vui, 06 Dec 2018 weaternblot, problem, IL36, Hacat	6 replies 835 views	Vui 09 Dec 2018
Answered 10% SDS Adjusting pH??? Started by Vui, 19 Nov 2018 SDS, pH, Adjusting	8 replies 910 views	mdfenko 29 Nov 2018
Answered western blotting troubleshooting Started by Vui, 08 Nov 2018 westernblot, smallprotein and 1 more...	3 replies 463 views	Vui 12 Nov 2018
Question on western blot picture Started by pito, 04 Sep 2018	2 replies 721 views	pito 06 Sep 2018
Lower band detected after alanine mutation Started by Thomson, 27 Aug 2018 western blot, protein and 2 more...	3 replies 1,585 views	Thomson 29 Aug 2018
Western-No bands for sample protein but loading control works Started by HawkeyeGrad, 12 Jul 2018 western blotprotein and 1 more...	5 replies 725 views	Artemis2007 13 Jul 2018
SDS-PAGE/2D gel and western blot for multiple proteins for multiple sample Started by Faisal SHAH, 05 Jul 2018 Electrophoresis and 2 more...	1 reply 635 views	mdfenko 06 Jul 2018
Severe or entire Blur formation in my SDS PAGE. Started by Siniya, 17 Apr 2018	1 reply 823 views	aimikins 17 Apr 2018
Severe or entire Blur formation in my SDS PAGE. Started by Siniya, 17 Apr 2018	0 replies 463 views	Siniya 17 Apr 2018
Standardization of SDS-PAGE!!! Started by Siniya, 04 Apr 2018	2 replies 821 views	mdfenko 05 Apr 2018
Sample buffer Started by Mad Researcher, 05 Feb 2018 sample buffer, DTT, Western Blot and 1 more...	1 reply 1,219 views	bob1 07 Feb 2018
How to prep mouse urine samples for SDS-PAGE and Coomassie staining? Started by esbena, 01 Dec 2017	2 replies 1,132 views	bob1 01 Dec 2017
WB imaging on Vilber-Lourmat Fusion Started by Trof, 25 Nov 2017	1 reply 968 views	bob1 28 Nov 2017
Forgot to block after stripping - please send help Started by NeuroG2019, 15 Nov 2017 stripping; western blot	1 reply 737 views	mdfenko 16 Nov 2017