Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

Submit your paper to J Biol Methods today!

J Biol Methods calls for papers

Journal of Biological Methods has published its first issue and is now calling for papers.

SDS-PAGE and Western Blotting

Please log in to post a topic
Mark this forum as read

Topic	Stats	Last Post Info
Pinned SDS-PAGE: casting Started by Doan Lan, 02 Jun 2002 polymerization, TEMED, APS 1 2	Hot 26 replies 17,660 views	shivangipurohit 27 Jun 2012
Pinned Cutting membrane after transfer Started by Pipette Dude, 01 Apr 2009 1 2	Hot 27 replies 16,421 views	mdfenko 07 May 2012
Pinned Western Blotting background troubleshooting Started by raje, 22 Jun 2004 1 2	Hot 22 replies 15,889 views	js86 29 Jun 2011
Pinned Tips on re-use of primary antibody in Western blot Started by jiro_killua, 08 Mar 2009	Hot 14 replies 13,392 views	ajxutao7 10 May 2011
Pinned x-ray films vs direct imaging Started by almost a doctor, 18 Jun 2009 1 2	Hot 17 replies 11,749 views	Inmost sun 19 Feb 2011
Pinned Western Blotting- A beginner Guide Started by Minnie Mouse, 01 Feb 2009	3 replies 10,048 views	GNANA 17 Sep 2010
Pinned semi-dry western conditions Started by flashboy, 25 Mar 2004	Hot 14 replies 11,593 views	kottila 04 Aug 2010
Pinned PVDF vs. Nitrocellulose for Western blot Started by Mr Jefferson, 31 Jan 2009 1 2	Hot 20 replies 35,245 views	mdfenko 04 Jun 2010
Pinned Old threads on SDS-PAGE and Western Blotting are achived here Started by bioforum, 03 Feb 2009	0 replies 5,744 views	bioforum 03 Feb 2009
Question on loss of signal between samples in western blot Started by Gradi, 26 Feb 2015 Western Blot, SDS PAGE, Proteins	4 replies 163 views	Gradi 27 Feb 2015
IP heavy chains not in Ponceau, but in final blot exposure Started by Hic5Prostate, 21 Feb 2015	1 reply 98 views	bob1 22 Feb 2015
Antibody-Biotinylation, detection via SDS-Gel Started by petertob, 02 Feb 2015 Antibody biotinylation and 4 more...	11 replies 515 views	mdfenko 18 Feb 2015
Some bands of similar intensity aren't seen in short exposure but are seen i Started by leighann36, 15 Feb 2015 western blot and 2 more...	1 reply 267 views	mdfenko 15 Feb 2015
Antibody Issues Started by Tayzz, 11 Feb 2015 Antibodies, HuH-7, Protein S and 1 more...	6 replies 191 views	mdfenko 12 Feb 2015
Why Glycomacropeptide (GMP) does not bind Coomassie Blue stain? Started by trungnghiatn, 05 Feb 2015	2 replies 130 views	trungnghiatn 05 Feb 2015
SDS page Started by Darlidada, 12 Jan 2015 western, gfp, antibody	8 replies 278 views	CPRES 30 Jan 2015
Is it possible to "over-block" a membrane? Started by science noob, 28 Jan 2015	5 replies 149 views	CPRES 30 Jan 2015
Troubleshooting for BN PAGE Started by neuron, 27 Jan 2015	4 replies 135 views	neuron 30 Jan 2015
Unspecific Western Signal from Protein A tag? Started by michael_p, 29 Jan 2015 Western Blotting	0 replies 406 views	michael_p 29 Jan 2015
SDS PAGE Destaining Started by Mag 22388, 21 Jan 2015 Destain	7 replies 323 views	mdfenko 26 Jan 2015
Answered Layer of water above separating gel mixes into gel Started by science noob, 19 Jan 2015	2 replies 178 views	CPRES 20 Jan 2015
A question about Gelanalyzer Started by trungnghiatn, 08 Jan 2015	8 replies 426 views	mdfenko 16 Jan 2015
PCR & Western Blot sample preperation Started by Homeostasis, 14 Jan 2015 Western blotting, PCR, analysis	2 replies 347 views	Homeostasis 15 Jan 2015
band obove the ladder Started by Perindu Bulan, 17 Dec 2014 electrophoresis, page	3 replies 455 views	CPRES 15 Jan 2015
Two-night primary antibody incubation? Started by Hic5Prostate, 10 Jan 2015	2 replies 267 views	CPRES 15 Jan 2015