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SDS-PAGE and Western Blotting

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Topic	Stats	Last Post Info
Pinned SDS-PAGE: casting Started by Doan Lan, 02 Jun 2002 polymerization, TEMED, APS 1 2	Hot 26 replies 16,008 views	shivangipurohit 27 Jun 2012
Pinned Cutting membrane after transfer Started by Pipette Dude, 01 Apr 2009 1 2	Hot 27 replies 15,549 views	mdfenko 07 May 2012
Pinned Western Blotting background troubleshooting Started by raje, 22 Jun 2004 1 2	Hot 22 replies 14,980 views	js86 29 Jun 2011
Pinned Tips on re-use of primary antibody in Western blot Started by jiro_killua, 08 Mar 2009	Hot 14 replies 12,726 views	ajxutao7 10 May 2011
Pinned x-ray films vs direct imaging Started by almost a doctor, 18 Jun 2009 1 2	Hot 17 replies 11,040 views	Inmost sun 19 Feb 2011
Pinned Western Blotting- A beginner Guide Started by Minnie Mouse, 01 Feb 2009	3 replies 9,472 views	GNANA 17 Sep 2010
Pinned semi-dry western conditions Started by flashboy, 25 Mar 2004	Hot 14 replies 11,154 views	kottila 04 Aug 2010
Pinned PVDF vs. Nitrocellulose for Western blot Started by Mr Jefferson, 31 Jan 2009 1 2	Hot 20 replies 33,818 views	mdfenko 04 Jun 2010
Pinned Old threads on SDS-PAGE and Western Blotting are achived here Started by bioforum, 03 Feb 2009	0 replies 5,406 views	bioforum 03 Feb 2009
Protein is present, but not detected by antibody Started by fomalhaut, 03 Mar 2014 western blot, antibody and 1 more...	3 replies 105 views	fomalhaut 04 Mar 2014
Answered Are certain tissues prone to uneven loading? Issues with intestine. Started by phrustrated, 25 Feb 2014 Intestine, liver, muscle	6 replies 122 views	phrustrated 26 Feb 2014
secondary antibody creation Started by luckyjade, 25 Feb 2014	1 reply 71 views	mdfenko 26 Feb 2014
loading control issue in blot with H9c2 cell protein, pleeeeeasee help Started by niveda, 24 Feb 2014	2 replies 67 views	NingLiu 25 Feb 2014
Incomplete Blotting and staining of NC membrane Started by TZ89, 25 Jan 2014 Western Blot, ELISA, Blotting and 2 more...	7 replies 219 views	TZ89 25 Feb 2014
Low signal, weird background stains on blot Started by alphawhiskey, 04 Feb 2014	5 replies 202 views	alphawhiskey 10 Feb 2014
high background with acetyl-lysine antibodies for IP immunoblot Started by crom80, 07 Feb 2014	0 replies 117 views	crom80 07 Feb 2014
Chemiluminescence vs. Fluorescence visualisation of western blot Started by science noob, 26 Jan 2014	1 reply 136 views	bob1 26 Jan 2014
identification of major protein; public 2D/mass-spec databases? Started by comp3v, 19 Jan 2014	2 replies 171 views	comp3v 19 Jan 2014
having problems with p-erk antibody and other phosphoproteins antibodies Started by rb213, 17 Jan 2014	1 reply 741 views	mdfenko 18 Jan 2014
Help, protein ladder didn`t separate Started by Seamus04, 14 Jan 2014	3 replies 166 views	mdfenko 15 Jan 2014
Determining phosphorylation status of a protein by transfection Started by Shamoon Saljuqi, 07 Jan 2014	5 replies 191 views	bob1 08 Jan 2014
Will 10 min boiling disrupt BS3 cross linking? Started by Sander, 22 Dec 2013 Immunoprecipitation	7 replies 279 views	neuron 26 Dec 2013
Problem with Denative lysis Started by thanhtam, 15 Dec 2013	1 reply 231 views	mdfenko 16 Dec 2013
Effect of MeOH and acetic acid on primary-secondary complex Started by Josip Madunic, 13 Dec 2013	3 replies 221 views	Josip Madunic 16 Dec 2013
Need help translating my western blot protocol into every language Started by doxorubicin, 03 Oct 2013 sds-page, western blot, language and 2 more...	3 replies 487 views	yangpengbo 14 Dec 2013