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SDS-PAGE and Western Blotting

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Topic	Stats	Last Post Info
Pinned SDS-PAGE: casting Started by Doan Lan, 02 Jun 2002 polymerization, TEMED, APS 1 2	Hot 26 replies 16,493 views	shivangipurohit 27 Jun 2012
Pinned Cutting membrane after transfer Started by Pipette Dude, 01 Apr 2009 1 2	Hot 27 replies 15,874 views	mdfenko 07 May 2012
Pinned Western Blotting background troubleshooting Started by raje, 22 Jun 2004 1 2	Hot 22 replies 15,372 views	js86 29 Jun 2011
Pinned Tips on re-use of primary antibody in Western blot Started by jiro_killua, 08 Mar 2009	Hot 14 replies 12,983 views	ajxutao7 10 May 2011
Pinned x-ray films vs direct imaging Started by almost a doctor, 18 Jun 2009 1 2	Hot 17 replies 11,335 views	Inmost sun 19 Feb 2011
Pinned Western Blotting- A beginner Guide Started by Minnie Mouse, 01 Feb 2009	3 replies 9,696 views	GNANA 17 Sep 2010
Pinned semi-dry western conditions Started by flashboy, 25 Mar 2004	Hot 14 replies 11,319 views	kottila 04 Aug 2010
Pinned PVDF vs. Nitrocellulose for Western blot Started by Mr Jefferson, 31 Jan 2009 1 2	Hot 20 replies 34,449 views	mdfenko 04 Jun 2010
Pinned Old threads on SDS-PAGE and Western Blotting are achived here Started by bioforum, 03 Feb 2009	0 replies 5,546 views	bioforum 03 Feb 2009
Western blot with smeared bands Started by lolifox, 24 Jul 2014	3 replies 248 views	mdfenko 25 Jul 2014
SDS PAGE sample preparation question, and discussion? Started by kyri_osity, 22 Jul 2014 electrophoresis, SDS PAGE and 1 more...	3 replies 278 views	mdfenko 24 Jul 2014
What is the cause of this Vertical smear in my western blot? Started by jcsslug, 22 Jul 2014	0 replies 155 views	jcsslug 22 Jul 2014
too much heating LDS-sample buffer Started by murusa, 17 Jul 2014 western blot, lds-sample buffer	3 replies 179 views	mdfenko 18 Jul 2014
Alkylation vastly changes the western blot signal of my protein Started by litrallis, 30 Jun 2014	0 replies 132 views	litrallis 30 Jun 2014
Viral Proteins Are Not Migrating Down the Gel Started by ACN, 19 Jun 2014 Viral proteins, Sendai and 3 more...	10 replies 296 views	mdfenko 26 Jun 2014
Protein is present, but not detected by antibody Started by fomalhaut, 03 Mar 2014 western blot, antibody and 1 more...	4 replies 361 views	CovanceABP 23 Jun 2014
problem of cell lysate Started by Xu Liu, 20 Jun 2014	3 replies 186 views	bob1 21 Jun 2014
western blotting running problem? Started by anna1103, 06 Jun 2014 western, problem	2 replies 284 views	mdfenko 10 Jun 2014
Blocking agent in western blotting of histone proteins Started by biochemscientist, 09 Jun 2014	1 reply 126 views	bob1 09 Jun 2014
Best western loading control for comparing different cell lines Started by Epigeneticist, 08 Jun 2014 Loading Control, Western Blotting	2 replies 162 views	Epigeneticist 08 Jun 2014
IgG heavy chain from IP Started by DrZeus, 05 May 2013 IP, rabbit, IgG, antibody, heavy	8 replies 1,126 views	mdfenko 06 Jun 2014
Keeping apoptotic/detached cells for WB? Started by mybioforumaccount, 05 Jun 2014	3 replies 163 views	bob1 05 Jun 2014
Advice with WB please Started by safe36, 02 Jun 2014	3 replies 249 views	safe36 03 Jun 2014
Western Blot Wet Transfer Conditions Started by steinpdh, 21 May 2014	10 replies 282 views	mdfenko 27 May 2014
I can detect my Flag-HA tagged protein with anti-HA but not anti-Flag Started by Epigeneticist, 02 May 2014 Western Blotting, Epitope Tag, HA and 2 more...	4 replies 309 views	Curtis 06 May 2014