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SDS-PAGE and Western Blotting

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Topic	Stats	Last Post Info
Pinned SDS-PAGE: casting Started by Doan Lan, 02 Jun 2002 polymerization, TEMED, APS 1 2	Hot 26 replies 14,931 views	shivangipurohit 27 Jun 2012
Pinned Cutting membrane after transfer Started by Pipette Dude, 01 Apr 2009 1 2	Hot 28 replies 14,674 views	mdfenko 07 May 2012
Pinned Western Blotting background troubleshooting Started by raje, 22 Jun 2004 1 2	Hot 22 replies 14,088 views	js86 29 Jun 2011
Pinned Tips on re-use of primary antibody in Western blot Started by jiro_killua, 08 Mar 2009	Hot 14 replies 12,072 views	ajxutao7 10 May 2011
Pinned x-ray films vs direct imaging Started by almost a doctor, 18 Jun 2009 1 2	Hot 17 replies 10,254 views	Inmost sun 19 Feb 2011
Pinned Western Blotting- A beginner Guide Started by Minnie Mouse, 01 Feb 2009	3 replies 8,858 views	GNANA 17 Sep 2010
Pinned semi-dry western conditions Started by flashboy, 25 Mar 2004	Hot 14 replies 10,730 views	kottila 04 Aug 2010
Pinned PVDF vs. Nitrocellulose for Western blot Started by Mr Jefferson, 31 Jan 2009 1 2	Hot 20 replies 31,188 views	mdfenko 04 Jun 2010
Pinned Old threads on SDS-PAGE and Western Blotting are achived here Started by bioforum, 03 Feb 2009	0 replies 5,073 views	bioforum 03 Feb 2009
Trouble with purified recombinant protein bands Started by Bioscientist, Yesterday, 07:44 PM	3 replies 32 views	mdfenko Today, 10:25 AM
Stain in electrophoresis is going from + to - Started by Gwynbleidd, 22 May 2013	3 replies 57 views	phage434 22 May 2013
Would IP make the protein of interest run slower on SDS-PAGE? Started by DrZeus, 21 May 2013 IP, SDS-PAGE, western, band, high	2 replies 71 views	mdfenko 22 May 2013
Extraction of Transmembrane Proteins Started by criscastells, 21 May 2013	1 reply 49 views	GNANA 21 May 2013
Western Blot - Quantifying Tumor samples from two different blots Started by youngscientist123456, 18 May 2013	2 replies 104 views	Iceage 20 May 2013
What is different between Native protein and Denatured protein samples. Started by Tai, 08 May 2013 western blot	4 replies 155 views	mdfenko 15 May 2013
Protocol for detecting soluble, secreted proteins on a Western? Started by JYaron, 13 May 2013 il-1b, western blot, caspase-1	1 reply 87 views	mdfenko 14 May 2013
Protein amount to load for publication figure? Started by HOYAJM, 10 May 2013	4 replies 125 views	HOYAJM 11 May 2013
3 extra bands show up on my western after an IP Started by DrZeus, 07 May 2013 ip, igg, heavy, western, mouse	2 replies 86 views	bob1 08 May 2013
Native gel electrophoresis: Can it tell if a protein is forming dimer? Started by Bioscientist, 08 May 2013	1 reply 83 views	bob1 08 May 2013
IgG heavy chain from IP Started by DrZeus, 05 May 2013 IP, rabbit, IgG, antibody, heavy	1 reply 171 views	bob1 05 May 2013
Uneven beta actin Started by asta6801, 15 Apr 2013 western blot, methods	2 replies 233 views	asta6801 30 Apr 2013
Western Blotting loading control for large proteins Started by alchemic, 30 Apr 2013	2 replies 144 views	alchemic 30 Apr 2013
Blue Native Gel - denaturing proteins after transfer? Started by Bobby007, 07 Aug 2009	9 replies 3,018 views	mdfenko 22 Apr 2013
Western blot antibody Started by weilun, 18 Apr 2013	0 replies 179 views	weilun 18 Apr 2013
NCOR1 western Started by abrahas, 09 Apr 2013	8 replies 281 views	mdfenko 15 Apr 2013