SDS-PAGE and Western Blotting

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Topic	Stats	Last Post Info
Pinned Cutting membrane after transfer Started by Pipette Dude, 01 Apr 2009 1 2	Hot 24 replies 11,776 views	science noob 05 Dec 2011
Pinned Tips on re-use of primary antibody in Western blot Started by jiro_killua, 08 Mar 2009	Hot 14 replies 10,177 views	ajxutao7 10 May 2011
Pinned Excess acrylamide in wells Started by Rob Steuart, 20 Apr 2009 1 2 3	Hot 31 replies 13,731 views	bioweapon 23 Nov 2010
Pinned Western Blotting- A beginner Guide Started by Minnie Mouse, 01 Feb 2009	3 replies 7,243 views	GNANA 17 Sep 2010
Pinned PVDF vs. Nitrocellulose for Western blot Started by Mr Jefferson, 31 Jan 2009 1 2	Hot 20 replies 22,761 views	mdfenko 04 Jun 2010
Pinned Multiple antibody blotting - western blot Started by gianluca, 09 Feb 2009 1 2	Hot 16 replies 11,070 views	basudec19 24 Jan 2010
Pinned Old threads on SDS-PAGE and Western Blotting are achived here Started by bioforum, 03 Feb 2009	0 replies 4,317 views	bioforum 03 Feb 2009
small protein blotting Started by proteinrunner, 22 Sep 2009	7 replies 2,324 views	cam 08 Feb 2012
Tris-Hcl becomes cloudy. Started by VJbiofication, 07 Feb 2012 SDS PAGE, electrophoresis	3 replies 50 views	miss-pcr 08 Feb 2012
Same non specific band for different target proteins Started by oak945, 18 Jan 2012 non specific band	1 reply 109 views	bithorax 07 Feb 2012
Birdlike bands/lanes of SDS-Page and WB Started by stefanie.grunwald@charite, 07 Feb 2012	2 replies 49 views	bob1 07 Feb 2012
Dot blot halo Started by sigfi, 18 Jan 2012 Dot blot, halo	4 replies 163 views	sigfi 06 Feb 2012
Can FLAG IP, but cannot detect with Western Started by CinogenCar, 02 Feb 2012 FLAG, IP, Western, western blot	4 replies 79 views	CinogenCar 03 Feb 2012
Native PAGE, interaction of proteins, validity Started by PeterJallerup, 26 Jan 2012	4 replies 122 views	mdfenko 01 Feb 2012
my protein bands and protein marker becomes abnormally clearer sometimes Started by joseant78, 31 Jan 2012 protein marker, clarify	3 replies 84 views	mdfenko 01 Feb 2012
Redoing only the Secondary Antibody staining on a Western Started by mightymouse, 28 Jan 2012 secondary, western, sds page gel	1 reply 119 views	bob1 29 Jan 2012
Trouble detecting Histaq Started by @bhijit, 16 Jan 2012	4 replies 163 views	mdfenko 25 Jan 2012
Smeared transfer on nitrocellulose membrane Started by biolcrazy, 24 Jan 2012	2 replies 114 views	biolcrazy 25 Jan 2012
Pull down assay and Co- IP experiment Started by luigialfano, 17 Jan 2012 Pull down assay, Co-IP	6 replies 206 views	luigialfano 23 Jan 2012
help: i accidentally use transfer buffer as running buffer, what will happen??? Started by zhuangyanyan, 18 Jan 2012	4 replies 185 views	mdfenko 20 Jan 2012
Types of 96-well plates used for BCA protein quantification Started by science noob, 18 Jan 2012	1 reply 112 views	bob1 19 Jan 2012
blocking and primary antibody in different buffers? Started by luciana, 19 Jan 2012	1 reply 128 views	bob1 19 Jan 2012
Western gels running super slow! Started by catgirl, 14 Jan 2012 western, gels	3 replies 215 views	mdfenko 19 Jan 2012
Western blot and isoelectric point Started by @bhijit, 17 Jan 2012	1 reply 129 views	mdfenko 19 Jan 2012
a band that suppose to be 90KD shows up at 70KD!! Started by HELP !!!, 13 Jan 2012	1 reply 156 views	mdfenko 19 Jan 2012