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Tissue and Cell Culture

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Topic	Stats	Last Post Info
storage of foetal calf serum Started by nube, 15 Aug 2012	3 replies 217 views	nube 16 Aug 2012
Calculation of Transfection Efficiency using SEAP reporter gene assay Started by Jaaar, 10 Jun 2012 SEAP, TransfectionCHO and 1 more...	0 replies 216 views	Jaaar 10 Jun 2012
culturing of freshly extracted PBMCs Started by dude3005, 03 Mar 2013 cell culture, pbmc	1 reply 216 views	science noob 04 Mar 2013
Glioma spheroids not invading Started by aikia, 07 Sep 2012	0 replies 215 views	aikia 07 Sep 2012
embrional mice liver Started by dusko123, 06 Dec 2011	0 replies 214 views	dusko123 06 Dec 2011
Addition of antibiotic to the culture medium Started by Mohamed samir, 05 Apr 2013	1 reply 214 views	Tabaluga 05 Apr 2013
MCF10A (ATCC) mammosphere 3D Started by rockbl, 18 May 2012	0 replies 212 views	rockbl 18 May 2012
Insect cells transfection reagent Started by macrox, 08 Feb 2013 transfection, Sf9, bacmid and 1 more...	1 reply 212 views	Curtis 10 Feb 2013
Mycoplasma on normal hands? Started by science noob, 11 Mar 2013	1 reply 212 views	bob1 11 Mar 2013
insulin concentration for tissue culture Started by SF_HK, 03 Sep 2012 calculation problems	3 replies 210 views	leelee 05 Sep 2012
Poll Cell numbers versus confluency Started by knuf, 23 Oct 2012	2 replies 210 views	leelee 23 Oct 2012
Dox induction and cell density Started by chabraha, 03 Jan 2013 NT2 cells, Doxycycline	0 replies 209 views	chabraha 03 Jan 2013
Community feedback needed to clarify terms for a Cell Culture Ontology Started by mhb120, 27 Mar 2013 Ontology, cell line and 1 more...	1 reply 209 views	bob1 02 Apr 2013
SF9 transfection with siRNA - what transfection reagent has worked for you? Started by Mai Doan, 02 Oct 2012 cell culture	3 replies 208 views	bob1 03 Oct 2012
Transfection of HeLas who seem stressed Started by madina(jnr), 02 May 2012 HeLa transfection, cell culture	0 replies 207 views	madina(jnr) 02 May 2012
Hybridoma development Started by amarnathbt, 30 Jun 2012	0 replies 206 views	amarnathbt 30 Jun 2012
airway primary cells stop growing at passage 3 Started by minhduc_bio, 12 Nov 2012	1 reply 206 views	bob1 12 Nov 2012
Primary culture of fibroblast cells from rat/mouse Started by wdyeo, 11 Apr 2013	4 replies 206 views	wdyeo 16 Apr 2013
How to isolate microglia from mouse brain Started by ismailelshimy, 09 May 2012 microglia isolation	0 replies 205 views	ismailelshimy 09 May 2012
help on cell type identification from mouse EBs Started by DavNu, 27 Mar 2012 huge cells, beating cells	0 replies 204 views	DavNu 27 Mar 2012
Human pancreatic and bone cancer cell lines for RGD targeting Started by asli, 07 May 2012	0 replies 204 views	asli 07 May 2012
cobalt chloride not inducing hypoxia Started by mansi@26, 28 Jul 2012	2 replies 204 views	bob1 29 Jul 2012
HEK293T cells are too fragile?? Started by science noob, 29 Aug 2012	2 replies 204 views	bob1 30 Aug 2012
HeLa growing cloudy Started by chandra3316, 10 Mar 2013 HeLa	4 replies 204 views	Tabaluga 12 Mar 2013
Need a protocol for Human Blood Lymphocyte Culture Started by Bill Manley, 11 Sep 2012	0 replies 203 views	Bill Manley 11 Sep 2012