PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

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Topic	Stats	Last Post Info
Primer design Started by seed, 12 Apr 2011	2 replies 781 views	seed 13 Apr 2011
Troubleshoot PCR, Product band missing, internal control is present Started by samanouske, 11 Apr 2011	1 reply 614 views	gt_ameya 11 Apr 2011
reaction efficiency Started by genetics3, 10 Apr 2011	4 replies 648 views	Trof 11 Apr 2011
How to determine the LOD for qPCR Started by hianghao, 11 Apr 2011	0 replies 493 views	hianghao 11 Apr 2011
Best way to mix qPCR master mix and high ct deviation questions Started by Stuck with Ligation, 05 Apr 2011	3 replies 645 views	vladooo 10 Apr 2011
qPCR volume Started by Polvon, 07 Apr 2011	1 reply 547 views	Trof 10 Apr 2011
software for quantifing PCR product band Started by deespike, 09 Apr 2011	1 reply 518 views	bob1 10 Apr 2011
Sybr Green Real Time PCR - Melt curve Started by siuchi98, 08 Apr 2011	3 replies 714 views	Maddie 09 Apr 2011
Thermal cycler Started by baboon, 08 Apr 2011	2 replies 701 views	Trof 08 Apr 2011
cDNA synthesis Started by Abiotic_Kabir, 03 Apr 2011	6 replies 961 views	Trof 08 Apr 2011
RT-PCR Started by seed, 06 Apr 2011	9 replies 864 views	BioMiha 08 Apr 2011
Do I need to pool the base line samples together Started by gingerman, 30 Mar 2011	2 replies 562 views	gingerman 07 Apr 2011
help with negative strand specific primer design needed Started by Andras, 05 Apr 2011	4 replies 631 views	Andras 06 Apr 2011
Will I need interplate calibration for my RT-PCR experiment? Started by cells, 02 Apr 2011	3 replies 764 views	Trof 05 Apr 2011
Few basic questions about RNA Started by Nephrite, 29 Mar 2011	3 replies 830 views	Trof 01 Apr 2011
Primer seq Started by noyara, 31 Mar 2011	2 replies 707 views	noyara 01 Apr 2011
Selection of housekeeping gene in Mycobacterium Started by jeff1234, 31 Mar 2011	1 reply 469 views	tea-test 31 Mar 2011
PCR triplicates versus one reaction Started by molbio1234, 21 Mar 2011 1 2	Hot 17 replies 1,075 views	Maddie 31 Mar 2011
PCR product size Started by seed, 30 Mar 2011	6 replies 632 views	almost a doctor 31 Mar 2011
Ct's of goi higher and lower than ct's hkg Started by vis, 28 Mar 2011	1 reply 599 views	Trof 30 Mar 2011
amplification plot raises to early Started by theo22, 30 Mar 2011	1 reply 472 views	Trof 30 Mar 2011
oligo dt and random primer Started by noyara, 28 Mar 2011	3 replies 718 views	noyara 30 Mar 2011
Effect of collagenase on qpcr results Started by pcrnewbie, 25 Mar 2011	1 reply 820 views	ivanbio 25 Mar 2011
RNA Isolation Started by vidz, 24 Mar 2011	3 replies 1,055 views	vidz 25 Mar 2011
conversion of total RNA to cDNA Started by shankares, 09 Feb 2011	7 replies 1,127 views	vidz 24 Mar 2011

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PCR, RT-PCR and Real-Time PCR

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