PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

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Topic	Stats	Last Post Info
Low RNA after chondrogenesis Started by gromlik, 12 May 2011	0 replies 361 views	gromlik 12 May 2011
Cleaning up RT-PCR product before sequencing Started by cells, 12 May 2011	1 reply 441 views	pDNA 12 May 2011
Improving qRT-PCR detection limits Started by Hriss, 12 May 2011	2 replies 461 views	Hriss 12 May 2011
What to use for Standard Curve Started by krisw, 11 May 2011	1 reply 466 views	Trof 12 May 2011
gPCR Started by chaperones, 11 May 2011	1 reply 516 views	tea-test 11 May 2011
RNA isolation from facs sorted mouse spleenocytes subsets Started by tongck1, 10 May 2011	0 replies 308 views	tongck1 10 May 2011
Real-time PCR about Incorrect setup of the data collection stage Started by yueh76, 09 May 2011	1 reply 411 views	Trof 09 May 2011
5' race Started by deespike, 28 Apr 2011	2 replies 443 views	burcin 09 May 2011
18s as endogenous control for Oligo dT RT Started by Panini, 06 May 2011	2 replies 593 views	Trof 08 May 2011
Primers... Started by chaperones, 07 May 2011	1 reply 487 views	bob1 07 May 2011
RT-PCR Normalization Started by Salem, 07 May 2011	0 replies 457 views	Salem 07 May 2011
excel and propagation of error, qpcr Started by thumbclaw, 06 May 2011	1 reply 520 views	tea-test 06 May 2011
water-droplets in my cycler !!! Started by nightingale, 26 Apr 2011	9 replies 799 views	neuropath 06 May 2011
primer with higher melting temperature Started by poorni_first, 05 May 2011	2 replies 475 views	Adrian K 05 May 2011
Omitting Points in Standard Curve Started by krisw, 04 May 2011	2 replies 505 views	krisw 05 May 2011
Reverse transcription (cDNA synthesis) curiosity and confusion Started by kedar, 05 May 2011	4 replies 574 views	Trof 05 May 2011
Smallest reaction volume with Roche Lightcycler 480 96-well plate format Started by neuropath, 30 Apr 2011	2 replies 511 views	neuropath 04 May 2011
When are primer dimers a problem? Started by BC Hop, 04 May 2011	0 replies 416 views	BC Hop 04 May 2011
Triage of miRNA targets by qPCR Started by iMatt, 30 Apr 2011	1 reply 547 views	Earnest 01 May 2011
qPCR primer design Started by Dr_D, 30 Apr 2011	1 reply 614 views	neuropath 30 Apr 2011
intron spanning primers for non model organisms Started by adtito, 13 Apr 2011	2 replies 446 views	adtito 30 Apr 2011
Primer dimer formation and real-time PCR Started by Nephrit, 29 Apr 2011	7 replies 625 views	Trof 30 Apr 2011
long range PCR Started by alk89, 28 Apr 2011	0 replies 463 views	alk89 28 Apr 2011
Maximum size of overhangs in PCR Started by seanspotatobusiness, 26 Apr 2011	6 replies 580 views	Rsm 28 Apr 2011
Illumina Eco real time PCR Started by Aly, 27 Apr 2011	0 replies 392 views	Aly 27 Apr 2011

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PCR, RT-PCR and Real-Time PCR

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