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PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

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Topic	Stats	Last Post Info
Lowered extension temps with Phusion Started by Warren, 19 Sep 2009	7 replies 3,569 views	alland 20 Mar 2010
Help with Real Time PCR Well To Well Variation Started by babylabrat, 15 Mar 2009	10 replies 3,463 views	Whiskeybent 18 Aug 2010
Why is it necessary to add Taq DNA polymerase last during PCR? Started by Chaste, 30 Mar 2009	8 replies 3,436 views	Prep! 26 Oct 2009
2 Tm peaks with SyBr experiment Started by poonamkd, 11 Jul 2009	6 replies 3,418 views	Ytje 23 Jul 2009
PCR product stays on agarose gel well !! Started by mmf, 30 Jun 2009	11 replies 3,406 views	mmf 09 Jul 2009
maximum amplicon size Started by moljul, 23 Feb 2009	5 replies 3,396 views	chrisbelle 06 Apr 2009
touchdown pcr Started by minty, 09 May 2009	6 replies 3,395 views	minty 12 May 2009
Etbr staining methods Started by mad, 05 Apr 2009	6 replies 3,394 views	swanny 10 May 2009
no pcr amplification product Started by lsb5579, 14 Sep 2009	9 replies 3,347 views	lsb5579 14 Oct 2009
SYBR and TaqMan Started by ellipis05, 13 Feb 2009	6 replies 3,299 views	dbiscoc 07 Apr 2010
Melting Curve Analysis Difficulties Started by BAZorko, 13 Feb 2009	7 replies 3,288 views	BAZorko 24 Feb 2009
Interpreting melting curve data in Sybr Green RT-PCR Started by pop09, 06 Nov 2009	12 replies 3,242 views	pop09 11 Nov 2009
RT-PCR primer design - Intron/exon boundaries Started by Hellie, 08 May 2009	6 replies 3,207 views	Hellie 12 May 2009
Genomic DNA copy number qPCR Started by miRNA man, 05 May 2009	8 replies 3,199 views	Kalpana Venkat Krishnan 20 Oct 2011
Genotyping Started by pmaj, 03 Aug 2009	6 replies 3,193 views	pmaj 06 Aug 2009
cDNA spectrophotometric quantification Started by AnnaSh, 25 Apr 2009	8 replies 3,171 views	littleaxt 28 Apr 2009
Can DEPC-water interfere QPCR reaction? Started by Thana, 17 Feb 2009	4 replies 3,162 views	ra_polymerase 27 Feb 2009
Shrimp nuclease to eliminate genomic DNA Started by willia, 07 Apr 2009	7 replies 3,160 views	Gerd 17 Apr 2009
Normalization of qpcr data Started by geetika, 01 Aug 2009	1 reply 3,151 views	chrisbelle 02 Aug 2009
pcr product quantification Started by deganuti, 15 Jul 2009	5 replies 3,112 views	ph1ll1ps 11 Aug 2009
New Technology in Real Time PCR Started by kate, 05 Feb 2009	2 replies 3,065 views	Unagi 06 Feb 2009
truble shooting qiagen 1 step rt pcr kit Started by rabos, 25 Jul 2009	2 replies 2,996 views	rabos 04 Aug 2009
how to design the primers for Real-Time PCR?? Started by bear, 06 Mar 2009	7 replies 2,969 views	molgen 15 Mar 2009
failed PCR on DNA extract from blood Started by logix, 24 Jun 2009	6 replies 2,968 views	KatieB 10 Jul 2009
long range PCR Started by trim5, 22 Sep 2009	9 replies 2,957 views	trim5 29 Sep 2009