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PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

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Topic	Stats	Last Post Info
qRT-PCR with DMSO Started by moerae, 03 May 2009	2 replies 1,624 views	alan6017518 28 May 2009
RNA contamination in PCR Started by alisonwks, 26 May 2009	1 reply 1,451 views	AquaPlasmid 28 May 2009
PCR without DNA extraction! Started by sarigsk, 25 May 2009	2 replies 1,418 views	warsel 28 May 2009
qPCR efficiency Started by tlp, 14 May 2009	4 replies 2,473 views	wuxx0153 26 May 2009
TaqMan real time PCR using abi 7700 system-troubleshooting Started by Jka83, 20 May 2009	5 replies 2,409 views	Jka83 25 May 2009
Primer dimers Started by littleaxt, 18 May 2009	5 replies 2,124 views	littleaxt 25 May 2009
RT-PCR Started by youyou, 20 May 2009	3 replies 1,612 views	littleaxt 25 May 2009
designing mini array experiment Started by zienpiggie, 24 May 2009	0 replies 825 views	zienpiggie 24 May 2009
PCR problems on a ligation product Started by ironman, 18 May 2009	5 replies 2,053 views	ironman 21 May 2009
acceptable range for ΔCt ? Started by Curtis, 12 May 2009	1 reply 1,279 views	Smonskey 20 May 2009
Genomic Contig Started by Sam80, 20 May 2009	0 replies 1,470 views	Sam80 20 May 2009
Ct fluctuations in qPCR Started by ela0405, 15 May 2009	3 replies 1,516 views	asdfyrr 18 May 2009
Contamination in negative control of AFLPs (PCRs) Started by klchisho, 17 May 2009	1 reply 1,095 views	klchisho 17 May 2009
touchdown pcr Started by minty, 09 May 2009	6 replies 3,396 views	minty 12 May 2009
RT-PCR vs. conventional PCR Started by medstudent1, 11 May 2009	1 reply 1,332 views	bob1 12 May 2009
Re-PCR problem Started by EcoliO157, 11 May 2009	3 replies 1,560 views	microgirl 12 May 2009
are my RT-primers ruined? accidentally stored at 4 C Started by medstudent1, 12 May 2009	1 reply 1,503 views	Vini 12 May 2009
RT-PCR primer design - Intron/exon boundaries Started by Hellie, 08 May 2009	6 replies 3,212 views	Hellie 12 May 2009
Etbr staining methods Started by mad, 05 Apr 2009	6 replies 3,399 views	swanny 10 May 2009
non specific pcr products Started by minty, 09 May 2009	4 replies 2,456 views	minty 10 May 2009
confirming concentration for primers in rt-pcr Started by Loris, 06 May 2009	2 replies 2,158 views	Curtis 09 May 2009
different Tm give different PCR product Started by fitriyusof, 09 May 2009	0 replies 1,310 views	fitriyusof 09 May 2009
template concentration qPCR Started by carry, 08 May 2009	1 reply 1,153 views	asdfyrr 08 May 2009
Gene-specific RT-PCR Started by Spl33n, 03 May 2009	2 replies 1,637 views	Spl33n 08 May 2009
difference between hotstart and taq polymerase Started by novagen, 26 Jan 2009	7 replies 2,870 views	Lazinase 07 May 2009