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PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

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Topic	Stats	Last Post Info
Within-group trend of decreasing mRNA expression Started by sven, 02 May 2012	0 replies 207 views	sven 02 May 2012
Taqman assay for detecting expression of genes carrying specific SNPs Started by panos.ioannidis@gmail.com, 26 Jul 2012	2 replies 207 views	Trof 28 Jul 2012
Polymerase for E. coli screenings Started by pDNA, 27 Jul 2012 polymerase	5 replies 206 views	pDNA 28 Jul 2012
Can't get PCR with large overhang primers to work Started by EtOH, 10 May 2013 PCR, overhang, low gc	8 replies 206 views	Makosad 14 May 2013
Different forward/reverse Primer concentration Started by Denise2910, 18 Aug 2012 telomere, primer concentration	0 replies 205 views	Denise2910 18 Aug 2012
qPCR [template] and [primer] troubleshooting...... Started by byobortolazzo, 09 Jan 2013	1 reply 205 views	Trof 15 Jan 2013
Taq polymerase Started by sercanpazarlar, 12 Mar 2013	7 replies 205 views	sercanpazarlar 13 Mar 2013
What does 'prevention of nontemplate-directed nucleotide addition' mean? Started by Curtis, 22 Nov 2012	3 replies 203 views	Trof 23 Nov 2012
qPCR from HEK cells transfected with Plasmid Started by cellthetruth, 01 Mar 2013 RNA, Plasmid	4 replies 202 views	Trof 04 Mar 2013
Real time PCR sudenly not working Started by eldem, 11 Jul 2012	1 reply 200 views	ashu2007 11 Jul 2012
how to make a working solution of a primers for pcr reaction?? Started by gourav88, 06 Feb 2013	4 replies 200 views	Tabaluga 10 Feb 2013
How 18s gene can be amplified when I use oligo dt for cDNA synthesis Started by vanotell, 07 Mar 2013 real time PCR, house keeping gene and 2 more...	4 replies 200 views	memari 10 Mar 2013
Will primers designed for RT-PCR amplification work on hybridoma germline DNA? Started by chan8, 12 Apr 2012 PCR, hybridoma, light chain and 2 more...	0 replies 199 views	chan8 12 Apr 2012
overlapping extention PCR problem Started by magnolia, 12 Jun 2012 overlapping PCR	0 replies 198 views	magnolia 12 Jun 2012
5'RACE of RNA Pol3 transcripts? Started by jaywilla, 13 Sep 2012 5RACE Pol2 Pol3	3 replies 198 views	Curtis 16 Sep 2012
Good Protocol for Semi-Quantitative RT-PCR? Started by ACN, 08 Jan 2013 qRT-PCR, Semi-Quantitative RT-PCR	1 reply 198 views	casandra 08 Jan 2013
Tm variation Started by bioclavire, 09 Jul 2012 real time	1 reply 196 views	Trof 15 Jul 2012
Calculating Standard Errors and Normalising to Transgene Started by Final year phd at wits end, 03 Feb 2013 standard errorr, transgene and 1 more...	1 reply 196 views	doxorubicin 04 Feb 2013
same primers, different product in conventional PCR and qPCR Started by alqga, 14 Aug 2012	6 replies 195 views	Trof 16 Aug 2012
low A260/230 problem Started by joy123, 27 Aug 2012	2 replies 195 views	joy123 28 Aug 2012
cDNA sequencing Started by Ameya P, 26 Sep 2012	2 replies 195 views	bob1 27 Sep 2012
Long range PCR optimization with consensus primers and different product lengths Started by sri lankan, 02 Dec 2012 Long range PCR, consensus primers	0 replies 195 views	sri lankan 02 Dec 2012
Amount of RNA (ng) for good ct values. Started by Wek, 06 Feb 2013	3 replies 195 views	jerryshelly1 18 Feb 2013
Help me out with Primer calculation for point mutagenesis.... Started by kartiga natarajan, 21 Feb 2013 Primer concentration	8 replies 195 views	GNANA 25 Feb 2013
PCR DNA Concentration Started by bmlmjone, 11 Apr 2013	0 replies 195 views	bmlmjone 11 Apr 2013