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PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

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Topic	Stats	Last Post Info
transient expression Started by aztecan princess, 01 Jun 2009	1 reply 1,154 views	pcrman 01 Jun 2009
Chosing the best housekeeping gene! Started by Curtis, 31 May 2009	5 replies 2,153 views	Star* 16 Jun 2009
real-time PCR and HIV Started by Opal, 30 May 2009	2 replies 1,450 views	Lazinase 04 Jun 2009
Unspecific PCR Started by Givi, 30 May 2009	7 replies 2,360 views	gebirgsziege 26 Jun 2009
Real-Time PCR as a Microplate Reader Started by alan6017518, 29 May 2009	1 reply 1,199 views	dtae 10 Mar 2011
RT-qPCR data analysis Started by kate, 28 May 2009	0 replies 1,400 views	kate 28 May 2009
RT-PCR Internal Standard Started by RRRColorado, 28 May 2009	2 replies 1,533 views	Rsm 29 May 2009
Differences in Thermal Cyclers? Started by Josefina, 28 May 2009	1 reply 1,205 views	dtimm 04 Jun 2009
ssDNA Quantification Started by alan6017518, 28 May 2009	4 replies 2,013 views	mastermi 07 Jun 2009
Standards for The Relative Standard Curve Method Started by Eon's mommy, 27 May 2009	3 replies 2,122 views	Eon's mommy 17 Jun 2009
RNA contamination in PCR Started by alisonwks, 26 May 2009	1 reply 1,451 views	AquaPlasmid 28 May 2009
DNase I pre-treatment Started by moljul, 26 May 2009	2 replies 1,426 views	lsek 31 May 2009
early ct value in ntc Started by harish, 25 May 2009	2 replies 1,370 views	harish 29 May 2009
PCR without DNA extraction! Started by sarigsk, 25 May 2009	2 replies 1,418 views	warsel 28 May 2009
designing mini array experiment Started by zienpiggie, 24 May 2009	0 replies 825 views	zienpiggie 24 May 2009
Primer tm Started by crackanaut, 22 May 2009	1 reply 1,409 views	mastermi 28 May 2009
Genomic Contig Started by Sam80, 20 May 2009	0 replies 1,470 views	Sam80 20 May 2009
TaqMan real time PCR using abi 7700 system-troubleshooting Started by Jka83, 20 May 2009	5 replies 2,409 views	Jka83 25 May 2009
RT-PCR Started by youyou, 20 May 2009	3 replies 1,612 views	littleaxt 25 May 2009
Long product amplification (1598bp) Started by doctorVolos, 19 May 2009	3 replies 1,459 views	mastermi 28 May 2009
PCR problems on a ligation product Started by ironman, 18 May 2009	5 replies 2,053 views	ironman 21 May 2009
Primer dimers Started by littleaxt, 18 May 2009	5 replies 2,124 views	littleaxt 25 May 2009
Contamination in negative control of AFLPs (PCRs) Started by klchisho, 17 May 2009	1 reply 1,096 views	klchisho 17 May 2009
Ct fluctuations in qPCR Started by ela0405, 15 May 2009	3 replies 1,516 views	asdfyrr 18 May 2009
qPCR efficiency Started by tlp, 14 May 2009	4 replies 2,474 views	wuxx0153 26 May 2009