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PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

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Topic	Stats	Last Post Info
Dye for qPCR Started by Mad researcher, 28 Mar 2013	3 replies 246 views	bigudukaz 28 Mar 2013
Normalize to cDNA for rtPCR? Started by dolphin12, 29 Jun 2012 rtPCR	0 replies 245 views	dolphin12 29 Jun 2012
Strange looking "elevated" amplification plots from ChIP-qPCR samples Started by CellBiologist, 27 Jan 2013	2 replies 245 views	jerryshelly1 06 Feb 2013
Giardia/Crypto real time PCR late amplification Started by dp4616, 12 Apr 2012	1 reply 244 views	Trof 13 Apr 2012
ssOligo control standard curve is not predicting ds genomic DNA concentration ac Started by katurne2, 11 Jun 2012 qPCR, genomic DNA, oligo and 2 more...	0 replies 243 views	katurne2 11 Jun 2012
PCR contamination Started by AGM1, 02 May 2012	0 replies 242 views	AGM1 02 May 2012
qPCR contamination problem Started by joy123, 29 Aug 2012	5 replies 242 views	Trof 31 Aug 2012
Restriction enzyme - PCR sheep blood gDNA Started by agorganic, 26 Nov 2012	5 replies 242 views	Trof 03 Dec 2012
RT-PCR product- no band Started by yagmur, 11 Apr 2013	4 replies 241 views	yagmur 15 Apr 2013
Problem related to Bcl-2 amplification and sequencing Started by litlbee, 01 May 2012 Bcl-2, sequencing	0 replies 238 views	litlbee 01 May 2012
Saliva and GCF DNA purification Started by Star 14, 02 Jun 2012 Bacterial DNA	1 reply 238 views	AquaPlasmid 06 Jun 2012
Universal ProbeLibrary for Human by ROCHE Started by gulash, 07 Dec 2012 roche, probe library	1 reply 238 views	Trof 07 Dec 2012
To design or use published primers? Started by science noob, 13 Mar 2013	4 replies 238 views	Trof 19 Mar 2013
Pair of primers (SYBR) with more than one amplicon product(?) Started by thinker, 27 Mar 2013	4 replies 236 views	phage434 28 Mar 2013
thermocycler - real-time versus conventional? Started by manta, 19 Jan 2013 pcr	2 replies 234 views	manta 19 Jan 2013
qPCR : why substracting CT values instead of dividing ? Started by Philosopher, 01 Aug 2012	3 replies 230 views	Philosopher 03 Aug 2012
Trouble correlating GU/ml qPCR results to cfu/ml plating results Started by schookp, 11 Jan 2013 qPCR, environmental qPCR, PCR and 1 more...	2 replies 230 views	phage434 22 Jan 2013
Suggestions, improvments for maps of 96-well plates on qPCR. Started by AlexandreB, 16 Jan 2013 qPCR, repeatibility and 3 more...	0 replies 229 views	AlexandreB 16 Jan 2013
LightCycler 480 Melting Curve Assay Started by fgentry, 30 Apr 2012	1 reply 228 views	Trof 03 May 2012
RT-qPCR plate set up NoRT controls Started by lylah, 15 Aug 2012	1 reply 228 views	Trof 16 Aug 2012
Electrophoresis problem , please help pals ! Started by M_shabani_medbiotech, 03 Feb 2013	8 replies 228 views	phage434 04 Feb 2013
Overlap PCR problem Started by HOYAJM, 16 Feb 2013 mutagenesis	5 replies 227 views	Curtis 20 Feb 2013
16s double bands obtained-why?? Started by sillysal, 31 May 2012	2 replies 226 views	sillysal 01 Jun 2012
Nonspecific Pfu product cleanup? Should I nest? Started by BioGeron32, 15 Jun 2012 Phusion, Products, Non-Specific and 2 more...	0 replies 226 views	BioGeron32 15 Jun 2012
Select the optimal primer-concentration Started by whitelines, 15 Feb 2013 real-time pcr, primers and 2 more...	0 replies 226 views	whitelines 15 Feb 2013