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PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

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Topic	Stats	Last Post Info
Design primer from incomplete sequence... Started by Saiful Shakirin, 04 Mar 2013 Primer	2 replies 137 views	Saiful Shakirin 04 Mar 2013
Long term storage for RNA extracted from pancreas Started by wdyeo, 03 Mar 2013	3 replies 122 views	jeff kwak 03 Mar 2013
RNA isolation from Sesame seed Started by m_biotech, 23 Feb 2013 RNA seed	3 replies 162 views	m_biotech 02 Mar 2013
How to perform colony PCR Started by freakyboy, 02 Mar 2013 DNA extraction	1 reply 175 views	Curtis 02 Mar 2013
DNA size band increased after gel electrophoresis with Quaigen fit Started by myandas, 27 Feb 2013 microbiology, PCR, purification	4 replies 214 views	myandas 27 Feb 2013
Recipe for TKM1 and TKM2 buffer for DNA isolation from Whole blood Started by srimadh, 27 Feb 2013 TKM1, TKM2, DNA isolation	1 reply 98 views	bob1 27 Feb 2013
Why RNase H treatment after cDNA synthesis Started by cellthetruth, 26 Feb 2013 RT-PCR, cDNA, RNase	2 replies 157 views	cellthetruth 26 Feb 2013
Help me out with Primer calculation for point mutagenesis.... Started by kartiga natarajan, 21 Feb 2013 Primer concentration	8 replies 199 views	GNANA 25 Feb 2013
Homogenize pancreas for RNA extraction Started by wdyeo, 21 Feb 2013	3 replies 159 views	wdyeo 23 Feb 2013
Gene expression from whole pancreas or islets by using RT-PCR Started by wdyeo, 21 Feb 2013	2 replies 160 views	wdyeo 23 Feb 2013
qPCR with Genomic DNA Started by klamc, 22 Feb 2013	1 reply 168 views	Multiplexion 22 Feb 2013
Point mutagenesis..... Started by kartiga natarajan, 19 Feb 2013 Stratagene reagents	5 replies 179 views	kartiga natarajan 20 Feb 2013
Overlap PCR problem Started by HOYAJM, 16 Feb 2013 mutagenesis	5 replies 231 views	Curtis 20 Feb 2013
No bands in PCR after DNase treatment Started by Christi, 18 Feb 2013	4 replies 165 views	jerryshelly1 19 Feb 2013
Amount of RNA (ng) for good ct values. Started by Wek, 06 Feb 2013	3 replies 202 views	jerryshelly1 18 Feb 2013
Select the optimal primer-concentration Started by whitelines, 15 Feb 2013 real-time pcr, primers and 2 more...	0 replies 233 views	whitelines 15 Feb 2013
PCR product purification Started by sri lankan, 14 Feb 2013 Purification, PCR products	4 replies 188 views	neuron 15 Feb 2013
acceptable melt curve? Started by joy123, 12 Feb 2013	1 reply 183 views	joy123 14 Feb 2013
Overlapping sequence PCR primers Started by biologg, 13 Feb 2013	1 reply 187 views	phage434 13 Feb 2013
Calibrator sample Started by marian18, 11 Feb 2013	4 replies 191 views	marian18 12 Feb 2013
No 5S RNA on a QC gel Started by PhD28, 12 Feb 2013	0 replies 138 views	PhD28 12 Feb 2013
DNA extraction - PCR Problem Started by Hamidrad, 04 Jan 2013	3 replies 288 views	vilperte 11 Feb 2013
how to make a working solution of a primers for pcr reaction?? Started by gourav88, 06 Feb 2013	4 replies 208 views	Tabaluga 10 Feb 2013
Good standard curve (maybe) but Ct values high Started by bjk1985, 08 Feb 2013	0 replies 192 views	bjk1985 08 Feb 2013
Long PCR (>10kb) polymerase recommendations Started by swanny, 23 Jan 2013 Long PCR, polymerase	3 replies 265 views	vilperte 08 Feb 2013