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PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

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Topic	Stats	Last Post Info
Buffers RNase decontamination Started by cecininí, 26 Mar 2013 RNases elimination	1 reply 107 views	bob1 26 Mar 2013
can canine COL1A1 primers be used to quantify human COL1A1 cDNA in qPCR Started by monts, 25 Mar 2013	1 reply 109 views	Trof 25 Mar 2013
Nested PCR Started by SarahLee8, 22 Mar 2013	2 replies 156 views	SarahLee8 25 Mar 2013
random primers or oligodT Started by SF_HK, 20 Mar 2013	4 replies 173 views	Trof 20 Mar 2013
Confused about normalization Started by SillySaru, 14 Mar 2013 qpcr, normalization, pfaffl	2 replies 261 views	memari 20 Mar 2013
To design or use published primers? Started by science noob, 13 Mar 2013	4 replies 239 views	Trof 19 Mar 2013
Reverse Transcriptase @ Room temperature for 48 hours Started by sowmi07, 26 Sep 2010	9 replies 776 views	Tabaluga 14 Mar 2013
Using RT-PCR to find the presence of a deletion in a gene Started by itaimo, 13 Mar 2013	2 replies 125 views	leelee 13 Mar 2013
Taq polymerase Started by sercanpazarlar, 12 Mar 2013	7 replies 206 views	sercanpazarlar 13 Mar 2013
How to compare gene expression in different cell lines Started by labgeek, 13 Mar 2013	0 replies 168 views	labgeek 13 Mar 2013
PCR primer usage (Clonning & cDNA) Started by Wilson0815, 12 Mar 2013	2 replies 129 views	DrRupesh Kumar Rajput 13 Mar 2013
Optimizing reactions for qRT-PCR using regular thermocycler Started by Neanderthal, 08 Mar 2013 qPCR optimization and 2 more...	4 replies 157 views	Trof 12 Mar 2013
How 18s gene can be amplified when I use oligo dt for cDNA synthesis Started by vanotell, 07 Mar 2013 real time PCR, house keeping gene and 2 more...	4 replies 203 views	memari 10 Mar 2013
The same CT with different Quantity, why? Started by Ensyeh, 06 Mar 2013	8 replies 183 views	doxorubicin 07 Mar 2013
reference gene for qPCR in E.coli Started by luxzo, 07 Mar 2013	1 reply 112 views	jerryshelly1 07 Mar 2013
Can you buy SYBR Green dye that is not in a master mix? Started by KatieBaker, 05 Mar 2013 SYBR Green	3 replies 152 views	KatieBaker 07 Mar 2013
Problem amplifying viral gene Started by @bhijit, 06 Mar 2013 PCR, electrophoresis, virus	5 replies 189 views	bob1 06 Mar 2013
Steps in performing qRT-PCR for microarray validation Started by Neanderthal, 06 Mar 2013 microarray validation and 4 more...	0 replies 128 views	Neanderthal 06 Mar 2013
Adding tag using overlapping PCR Started by im0404, 05 Mar 2013 PCR	2 replies 154 views	im0404 06 Mar 2013
Negative Control Looks Like the Rest Started by smndtupidisaftr, 26 Feb 2013	11 replies 213 views	bob1 05 Mar 2013
qPCR from HEK cells transfected with Plasmid Started by cellthetruth, 01 Mar 2013 RNA, Plasmid	4 replies 203 views	Trof 04 Mar 2013
Design primer from incomplete sequence... Started by Saiful Shakirin, 04 Mar 2013 Primer	2 replies 136 views	Saiful Shakirin 04 Mar 2013
Long term storage for RNA extracted from pancreas Started by wdyeo, 03 Mar 2013	3 replies 121 views	jeff kwak 03 Mar 2013
RNA isolation from Sesame seed Started by m_biotech, 23 Feb 2013 RNA seed	3 replies 161 views	m_biotech 02 Mar 2013
How to perform colony PCR Started by freakyboy, 02 Mar 2013 DNA extraction	1 reply 173 views	Curtis 02 Mar 2013