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PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

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Topic	Stats	Last Post Info
UNG in PCR Started by xxcici, 05 May 2013	1 reply 94 views	bob1 05 May 2013
Template DNA for PCR- Concrentration or Volume?? Started by Hockey4496, 01 May 2013	1 reply 123 views	bob1 01 May 2013
A question for Reverse transcription experts Started by Mohamed samir, 25 Apr 2013	5 replies 174 views	Mohamed samir 26 Apr 2013
urgent: need help with qPCR statistics Started by joy123, 26 Apr 2013	2 replies 138 views	joy123 26 Apr 2013
the storage time for primers Started by xxcici, 26 Mar 2013	9 replies 299 views	sikander 24 Apr 2013
FAM and VIC S-curves Started by squallweathered, 23 Apr 2013 qPCR, FAM, VIC, DNA, RNA	0 replies 164 views	squallweathered 23 Apr 2013
qPCR for telomere length measurement - efficiency issues Started by dtae, 17 Aug 2010 1 2 3	Hot 40 replies 5,854 views	Langas12 23 Apr 2013
will mRNA from different cell line lead to loss of expected product in RT PCR? Started by Indhumathi, 17 Apr 2013	2 replies 215 views	Indhumathi 17 Apr 2013
using cell line to generate standard curve Started by detriar, 14 Apr 2013 cell line, standard curve and 1 more...	5 replies 256 views	bigudukaz 16 Apr 2013
PCR amplification with new restriction sites troubleshooting Started by Suzanna, 15 Apr 2013	2 replies 164 views	Suzanna 16 Apr 2013
RT-PCR product- no band Started by yagmur, 11 Apr 2013	4 replies 243 views	yagmur 15 Apr 2013
Standard curve using plasmid Started by thekid, 23 Apr 2012 Plasmid standard	1 reply 338 views	detriar 14 Apr 2013
PCR DNA Concentration Started by bmlmjone, 11 Apr 2013	0 replies 196 views	bmlmjone 11 Apr 2013
3' RACE creates too big band doesn't leave well Started by ShanaK, 11 Apr 2013	1 reply 110 views	bob1 11 Apr 2013
Normalization factor Started by Mad researcher, 06 Apr 2013	3 replies 254 views	Mad researcher 09 Apr 2013
SYBR Select Master Mix for CFX from Invitrogen - Storage Started by toprak, 29 Mar 2013	4 replies 355 views	Trof 08 Apr 2013
Understanding RACE PCR Started by Ubiquitous, 31 Mar 2013	1 reply 269 views	Trof 08 Apr 2013
Which High-Fidelity polymerase is better? Started by Guf, 08 Mar 2013 PCR, High-Fidelity, Polymerase	2 replies 277 views	bigudukaz 05 Apr 2013
Annealing temperature for PCR Started by Meng Li, 26 Mar 2013	8 replies 429 views	bigudukaz 04 Apr 2013
PCR with Plasmid recovered from filter paper Started by Indhumathi, 26 Mar 2013	6 replies 363 views	Indhumathi 01 Apr 2013
Dye for qPCR Started by Mad researcher, 28 Mar 2013	3 replies 248 views	bigudukaz 28 Mar 2013
Wierd Bands after PCR....Confused Started by Cathy1, 27 Mar 2013 PCR	9 replies 272 views	Cathy1 28 Mar 2013
Pair of primers (SYBR) with more than one amplicon product(?) Started by thinker, 27 Mar 2013	4 replies 236 views	phage434 28 Mar 2013
PCR amplified product size Started by Saiful Shakirin, 26 Mar 2013 PCR	5 replies 306 views	jerryshelly1 27 Mar 2013
Opinion: What fold change is actually considered meaningful Started by wcsoccerfan, 26 Mar 2013	2 replies 129 views	pcrman 26 Mar 2013