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PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

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Topic	Stats	Last Post Info
Homogenizing animal tissue to RT-PCR in real-time Started by Lina Salazar, 16 Sep 2009	2 replies 1,456 views	AussieUSA 18 Sep 2009
RT-PCR Started by kdavies9, 17 Sep 2009	2 replies 1,059 views	gleb.kudr 18 Sep 2009
Multiplex PCR Started by mihika, 16 Sep 2009	1 reply 1,010 views	gleb.kudr 17 Sep 2009
Freezing primers+SYBR green Started by scienceyay, 14 Sep 2009	2 replies 1,325 views	gleb.kudr 17 Sep 2009
Amplfication of fungal DNA Started by chat25, 08 Sep 2009	8 replies 2,709 views	Adrian K 15 Sep 2009
TaqMan qRTPCR amplification oddity Started by glenk, 15 Sep 2009	0 replies 674 views	glenk 15 Sep 2009
Real-time PCR assay design for prokaryotes Started by Monkeyguy, 14 Sep 2009	0 replies 857 views	Monkeyguy 14 Sep 2009
Mysterious PCR Contamination Started by IYM, 02 Sep 2009	13 replies 3,730 views	swanny 13 Sep 2009
less steep curve and high Ct value! Started by hzauhwj, 09 Sep 2009	2 replies 1,801 views	hzauhwj 11 Sep 2009
PCR problem Started by xiaoposhi, 08 Sep 2009	4 replies 2,324 views	xiaoposhi 10 Sep 2009
Use of iScript RT from Biorad Started by sameerjoshi, 10 Sep 2009	0 replies 841 views	sameerjoshi 10 Sep 2009
Gain! Started by Curtis, 30 Apr 2009	4 replies 1,931 views	RatRace2000 10 Sep 2009
change in ct value due to tube placement Started by thekid, 10 Sep 2009	0 replies 764 views	thekid 10 Sep 2009
optimal template dna concentrations Started by sri lankan, 06 Sep 2009	1 reply 1,433 views	Adrian K 09 Sep 2009
Multiplex PCR Started by lysjoshua, 08 Sep 2009	1 reply 873 views	Adrian K 09 Sep 2009
low raw fluorescence Started by sponge_nuts, 09 Sep 2009	0 replies 1,083 views	sponge_nuts 09 Sep 2009
Low qPCR efficiency with plasmid template, why? Started by aphroditeH, 19 Aug 2009	5 replies 2,097 views	aphroditeH 09 Sep 2009
mRNA level in transgene cell line or knocking down cell line Started by ucsd, 08 Sep 2009	3 replies 1,408 views	Dr Teeth 09 Sep 2009
Question about site directed mutagenesis Started by cupidstunt, 09 Sep 2009	1 reply 853 views	Dr Teeth 09 Sep 2009
No amplification in PCR using gDNA extracted from formailin fixed paraffin embed Started by FFPE1, 07 Sep 2009	3 replies 2,247 views	gebirgsziege 08 Sep 2009
differently behavioring replicates in real-time PCR Started by egerecske, 08 Sep 2009	1 reply 1,014 views	almost a doctor 08 Sep 2009
PCR-how to set 'Extension: ramping from 55 to 72 for 5min' Started by fortunate, 31 Aug 2009	4 replies 1,779 views	swanny 07 Sep 2009
Questions about SYBR Green Started by romero2009, 07 Sep 2009	0 replies 862 views	romero2009 07 Sep 2009
reconstitution of primers Started by chat25, 03 Sep 2009	2 replies 1,584 views	chat25 03 Sep 2009
Different melting curve profiles Started by Crusader, 02 Sep 2009	0 replies 970 views	Crusader 02 Sep 2009