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PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

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Topic	Stats	Last Post Info
Normalizing qPCR data Started by Epigeneticist, 05 Feb 2013 Statistics, Normalizing, SD, qPCR	1 reply 223 views	bob1 06 Feb 2013
Calculating Standard Errors and Normalising to Transgene Started by Final year phd at wits end, 03 Feb 2013 standard errorr, transgene and 1 more...	1 reply 197 views	doxorubicin 04 Feb 2013
Electrophoresis problem , please help pals ! Started by M_shabani_medbiotech, 03 Feb 2013	8 replies 229 views	phage434 04 Feb 2013
add template first or Syber mix first to reduce contamination in qPCR? Started by joy123, 27 Jan 2013	3 replies 221 views	jerryshelly1 02 Feb 2013
PCR-screening of T-DNA inserted in Arabidopsis gene Started by Gwynbleidd, 25 Jan 2013	1 reply 175 views	bob1 26 Jan 2013
iCycler Calibration Question Started by HOYAJM, 24 Jan 2013 SYBR green, iCycler	1 reply 180 views	HOYAJM 24 Jan 2013
PCR band slightly BELOW expected length ?! Started by Tabaluga, 14 Jan 2013 PCR, band	Hot 14 replies 342 views	Tabaluga 24 Jan 2013
Trouble correlating GU/ml qPCR results to cfu/ml plating results Started by schookp, 11 Jan 2013 qPCR, environmental qPCR, PCR and 1 more...	2 replies 230 views	phage434 22 Jan 2013
Change in Superscript lot influences transcript level of one gene but not others Started by dpo, 18 Jan 2013	2 replies 194 views	dpo 21 Jan 2013
I need help for designing a pair of primers for this sequence. Started by chaghaleh, 18 Jan 2013 Primer designing	1 reply 249 views	Trof 21 Jan 2013
thermocycler - real-time versus conventional? Started by manta, 19 Jan 2013 pcr	2 replies 234 views	manta 19 Jan 2013
1/delta Ct vs delta delat CT Started by SF_HK, 17 Jan 2013	1 reply 183 views	Trof 17 Jan 2013
Suggestions, improvments for maps of 96-well plates on qPCR. Started by AlexandreB, 16 Jan 2013 qPCR, repeatibility and 3 more...	0 replies 229 views	AlexandreB 16 Jan 2013
qPCR [template] and [primer] troubleshooting...... Started by byobortolazzo, 09 Jan 2013	1 reply 208 views	Trof 15 Jan 2013
long primers PCR Started by volver, 10 Jan 2013	4 replies 181 views	Trof 15 Jan 2013
Does anyone have any problem with Quanta Sybr Green Started by scardoso, 11 Jan 2013 Sybr GreenReal-time PCR	2 replies 211 views	scardoso 15 Jan 2013
Tagged primers and a second PCR targetting the tags Started by sri lankan, 18 Dec 2012 Primers	5 replies 305 views	sri lankan 14 Jan 2013
unexpected band in PCR with plasmid Started by Indhu, 10 Jan 2013	4 replies 164 views	doxorubicin 11 Jan 2013
Multiple Bands in qPCR Started by AlexanderA, 21 Dec 2012 Primer, multiple Bands, qPCR	4 replies 420 views	AlexanderA 10 Jan 2013
Real Time Trouble, low cTs Started by KenGoh, 04 Jan 2013	3 replies 269 views	doxorubicin 09 Jan 2013
Good Protocol for Semi-Quantitative RT-PCR? Started by ACN, 08 Jan 2013 qRT-PCR, Semi-Quantitative RT-PCR	1 reply 199 views	casandra 08 Jan 2013
interpretation of results Started by ozeir, 30 Dec 2012	8 replies 371 views	ozeir 07 Jan 2013
Question about isolating DNA from the blood sample Started by mightymus, 18 Dec 2012 qpcr, dna extraction from blo	2 replies 344 views	mightymus 28 Dec 2012
Is it possible to compare abundance of isoforms by qPCR Started by gyma, 06 Dec 2012 qPCR, isoform, efficiency, gene	6 replies 416 views	gyma 27 Dec 2012
isolating DNA from mouse tails and subsequent problems with pcr Started by sligeach, 29 Nov 2012	7 replies 422 views	Myworkismyplay 26 Dec 2012