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PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

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Topic	Stats	Last Post Info
Small product amplification problems Started by Hal, 16 Sep 2009	4 replies 1,242 views	gleb.kudr 18 Sep 2009
Tagged Primers Started by Ivan, 14 Sep 2009	5 replies 1,796 views	Ivan 18 Sep 2009
Two annealing temps? Started by microgirl, 17 Sep 2009	4 replies 1,665 views	LabDiagMol 18 Sep 2009
Homogenizing animal tissue to RT-PCR in real-time Started by Lina Salazar, 16 Sep 2009	2 replies 1,498 views	AussieUSA 18 Sep 2009
RT-PCR Started by kdavies9, 17 Sep 2009	2 replies 1,082 views	gleb.kudr 18 Sep 2009
Multiplex PCR Started by mihika, 16 Sep 2009	1 reply 1,054 views	gleb.kudr 17 Sep 2009
Freezing primers+SYBR green Started by scienceyay, 14 Sep 2009	2 replies 1,353 views	gleb.kudr 17 Sep 2009
Amplfication of fungal DNA Started by chat25, 08 Sep 2009	8 replies 2,755 views	Adrian K 15 Sep 2009
TaqMan qRTPCR amplification oddity Started by glenk, 15 Sep 2009	0 replies 693 views	glenk 15 Sep 2009
Real-time PCR assay design for prokaryotes Started by Monkeyguy, 14 Sep 2009	0 replies 864 views	Monkeyguy 14 Sep 2009
Mysterious PCR Contamination Started by IYM, 02 Sep 2009	13 replies 3,774 views	swanny 13 Sep 2009
less steep curve and high Ct value! Started by hzauhwj, 09 Sep 2009	2 replies 1,816 views	hzauhwj 11 Sep 2009
PCR problem Started by xiaoposhi, 08 Sep 2009	4 replies 2,442 views	xiaoposhi 10 Sep 2009
Use of iScript RT from Biorad Started by sameerjoshi, 10 Sep 2009	0 replies 852 views	sameerjoshi 10 Sep 2009
Gain! Started by Curtis, 30 Apr 2009	4 replies 1,983 views	RatRace2000 10 Sep 2009
change in ct value due to tube placement Started by thekid, 10 Sep 2009	0 replies 783 views	thekid 10 Sep 2009
optimal template dna concentrations Started by GradMicro, 06 Sep 2009	1 reply 1,450 views	Adrian K 09 Sep 2009
Multiplex PCR Started by lysjoshua, 08 Sep 2009	1 reply 903 views	Adrian K 09 Sep 2009
low raw fluorescence Started by sponge_nuts, 09 Sep 2009	0 replies 1,110 views	sponge_nuts 09 Sep 2009
Low qPCR efficiency with plasmid template, why? Started by aphroditeH, 19 Aug 2009	5 replies 2,139 views	aphroditeH 09 Sep 2009
mRNA level in transgene cell line or knocking down cell line Started by ucsd, 08 Sep 2009	3 replies 1,460 views	Dr Teeth 09 Sep 2009
Question about site directed mutagenesis Started by cupidstunt, 09 Sep 2009	1 reply 891 views	Dr Teeth 09 Sep 2009
No amplification in PCR using gDNA extracted from formailin fixed paraffin embed Started by FFPE1, 07 Sep 2009	3 replies 2,277 views	gebirgsziege 08 Sep 2009
differently behavioring replicates in real-time PCR Started by egerecske, 08 Sep 2009	1 reply 1,058 views	almost a doctor 08 Sep 2009
PCR-how to set 'Extension: ramping from 55 to 72 for 5min' Started by fortunate, 31 Aug 2009	4 replies 1,832 views	swanny 07 Sep 2009