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PCR, RT-PCR and Real-Time PCR

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Topic	Stats	Last Post Info
Run PCR amplifications in agarose gels Started by criscastells, 03 May 2010	3 replies 2,214 views	criscastells 07 May 2010
Analysis HELP!!!! Started by ciliate, 29 Apr 2010	1 reply 1,256 views	paramitay 06 May 2010
High Amplification Efficiency in Std. Curve Started by ColoSarah, 05 May 2010	5 replies 2,342 views	paramitay 06 May 2010
High Ct value - what to do next? Started by wntiong, 22 Apr 2010	4 replies 2,323 views	paramitay 06 May 2010
Biorad Mini Opticon Started by chrisbelle, 22 Apr 2010	1 reply 1,576 views	klasfahl 06 May 2010
PCR/RT-PCR beads Started by wtterb, 05 May 2010	2 replies 1,650 views	wtterb 06 May 2010
[Help]universal tag Started by bear23456, 28 Apr 2010	4 replies 1,834 views	bear23456 06 May 2010
E. coli 16S or uidA? Started by lauren.peterson, 05 May 2010	0 replies 1,166 views	lauren.peterson 05 May 2010
Relative RT-PCR, multiplate and calibration Started by rajah, 03 Mar 2010	4 replies 2,166 views	rajah 05 May 2010
Problems with validating primers and low expression genes Started by Fran24, 03 May 2010	3 replies 2,138 views	Trof 04 May 2010
PCR product dimer issue Started by rudzielec, 08 Apr 2010 1 2	Hot 24 replies 4,975 views	rudzielec 03 May 2010
General real time questions. Started by ColoSarah, 30 Apr 2010	1 reply 1,269 views	tea-test 02 May 2010
Which method is best for DNA quantification? Started by mwalt2, 29 Apr 2010	0 replies 1,279 views	mwalt2 29 Apr 2010
Mutagenesis woes Started by georgiadave, 27 Apr 2010	3 replies 1,521 views	ProteinWork 27 Apr 2010
KpnI Restriction enzyme Started by maxmix, 27 Apr 2010	1 reply 1,490 views	Curtis 27 Apr 2010
LC3 RT-PCR primers Started by holyblood, 27 Apr 2010	0 replies 1,152 views	holyblood 27 Apr 2010
PCR product for sequencing Started by tashaD, 25 Apr 2010	2 replies 1,407 views	Micro 26 Apr 2010
can I use routine PCR to assess whether a gene is expressed or not? Started by fzhang, 26 Apr 2010	3 replies 1,534 views	Rsm 26 Apr 2010
Relative gene expression Started by ColoSarah, 16 Apr 2010	3 replies 1,864 views	Trof 26 Apr 2010
Absolute quantification using Real Time PCR Started by chn09, 25 Apr 2010	2 replies 1,415 views	Trof 26 Apr 2010
intron/exon spanning primers Started by SF_HK, 26 Apr 2010	1 reply 1,530 views	Trof 26 Apr 2010
GAPDH CT lower than target gene CT Started by SF_HK, 19 Apr 2010	2 replies 1,898 views	SF_HK 26 Apr 2010
variable endogenous control Started by probe_10, 24 Apr 2010	0 replies 1,095 views	probe_10 24 Apr 2010
PCR of AT rich gene - Started by sfl40, 23 Apr 2010	4 replies 1,792 views	sfl40 24 Apr 2010
qRT-PCR calculation question Started by schmidthead, 22 Apr 2010	0 replies 1,068 views	schmidthead 22 Apr 2010