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PCR, RT-PCR and Real-Time PCR
Questions related to any PCR, primers etc.
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High Amplification Efficiency in Std. CurveStarted by ColoSarah, 05 May 2010 |
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High Ct value - what to do next?Started by wntiong, 22 Apr 2010 |
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Biorad Mini OpticonStarted by chrisbelle, 22 Apr 2010 |
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PCR/RT-PCR beadsStarted by wtterb, 05 May 2010 |
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[Help]universal tagStarted by bear23456, 28 Apr 2010 |
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E. coli 16S or uidA?Started by lauren.peterson, 05 May 2010 |
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Relative RT-PCR, multiplate and calibrationStarted by rajah, 03 Mar 2010 |
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Problems with validating primers and low expression genesStarted by Fran24, 03 May 2010 |
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PCR product dimer issueStarted by rudzielec, 08 Apr 2010 |
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General real time questions.Started by ColoSarah, 30 Apr 2010 |
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Which method is best for DNA quantification?Started by mwalt2, 29 Apr 2010 |
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Mutagenesis woesStarted by georgiadave, 27 Apr 2010 |
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KpnI Restriction enzymeStarted by maxmix, 27 Apr 2010 |
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LC3 RT-PCR primersStarted by holyblood, 27 Apr 2010 |
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PCR product for sequencingStarted by tashaD, 25 Apr 2010 |
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can I use routine PCR to assess whether a gene is expressed or not?Started by fzhang, 26 Apr 2010 |
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Relative gene expressionStarted by ColoSarah, 16 Apr 2010 |
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Absolute quantification using Real Time PCRStarted by chn09, 25 Apr 2010 |
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intron/exon spanning primersStarted by SF_HK, 26 Apr 2010 |
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GAPDH CT lower than target gene CTStarted by SF_HK, 19 Apr 2010 |
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variable endogenous controlStarted by probe_10, 24 Apr 2010 |
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PCR of AT rich gene -Started by sfl40, 23 Apr 2010 |
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qRT-PCR calculation questionStarted by schmidthead, 22 Apr 2010 |
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Pre-amplification of template for qPCRStarted by echica, 13 Apr 2010 |
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more than one bandStarted by ARR, 16 Apr 2010 |
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