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PCR, RT-PCR and Real-Time PCR
Questions related to any PCR, primers etc.
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Reference gene(s) selection and validaton for qPCRStarted by Trof, 22 Apr 2014  housekeeping, normalization and 1 more...
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Inexplicable qPCR failure in single wellsStarted by Tabaluga, 18 Aug 2013 qPCR, real-time PCR, failure
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Real-Time RT-PCR one-step and two step issueStarted by Curtis, 26 Feb 2009 |
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What went wrong in my qPCR?Started by molecular_medicine, 19 Feb 2009 |
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How & where to prepare qPCRStarted by goyabean, 10 Aug 2009 |
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Primer dimer issue in real time PCRStarted by veer2, 21 Jun 2009 |
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PCR product purificationStarted by thegradstudent, 01 Apr 2004 |
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Smear in long distance PCRStarted by xabiarias, 02 Mar 2011 |
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PCR Contamination issuesStarted by Martorse, 01 Oct 2004 pcr, contamination
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Important literature for real time PCR usersStarted by tea-test, 29 Jan 2009 |
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How to get rid of PCR primer dimerStarted by bouttela, 17 Mar 2003 pcr, dimmer
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Understanding qPCR and gene expression changesStarted by Sciencefreak, 31 Mar 2009 |
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Normalization for RNA or cDNA during two step RT-PCR?Started by Curtis, 20 Apr 2009 |
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what may have gone wrong?Started by yobou, 05 Jun 2018 |
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Problem with cDNA synthesisStarted by shwetaT, 31 Jul 2018 |
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Trouble with PCR after cloningStarted by Saadlee, 01 Aug 2018 PCR, cloning
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Mastermix expiry dates?Started by AKB, 30 Apr 2018 qPCR, expiry date, mastermix and 2 more...
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bands are not discrete in PCR, why?Started by yobou, 30 May 2018 |
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real-time PCR machine errorStarted by dnasequencer, 03 Jul 2018 |
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normalisation of control samples to "1"Started by erb, 15 Jul 2018 |
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Reverse Transcriptase @ Room temperature for 48 hoursStarted by sowmi07, 26 Sep 2010 |
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Verifying positive during PCR amplificationStarted by Junguk Ko, 20 May 2018 qPCR, DYE, PCR, Gel green, SYBR
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StepOne Plus problems!Started by eastelzer, 18 May 2018 StepOne Plus
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how to predict amplicon size?Started by yobou, 09 May 2018 |
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Optimization of real time-PCR - where should I start from?Started by Nephrite, 17 Apr 2018 |
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