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PCR, RT-PCR and Real-Time PCR
Questions related to any PCR, primers etc.
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Reference gene(s) selection and validaton for qPCRStarted by Trof, 22 Apr 2014  housekeeping, normalization and 1 more...
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Inexplicable qPCR failure in single wellsStarted by Tabaluga, 18 Aug 2013 qPCR, real-time PCR, failure
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Real-Time RT-PCR one-step and two step issueStarted by Curtis, 26 Feb 2009 |
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What went wrong in my qPCR?Started by molecular_medicine, 19 Feb 2009 |
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How & where to prepare qPCRStarted by goyabean, 10 Aug 2009 |
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Primer dimer issue in real time PCRStarted by veer2, 21 Jun 2009 |
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PCR product purificationStarted by thegradstudent, 01 Apr 2004 |
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Smear in long distance PCRStarted by xabiarias, 02 Mar 2011 |
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PCR Contamination issuesStarted by Martorse, 01 Oct 2004 pcr, contamination
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Important literature for real time PCR usersStarted by tea-test, 29 Jan 2009 |
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How to get rid of PCR primer dimerStarted by bouttela, 17 Mar 2003 pcr, dimmer
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Understanding qPCR and gene expression changesStarted by Sciencefreak, 31 Mar 2009 |
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Normalization for RNA or cDNA during two step RT-PCR?Started by Curtis, 20 Apr 2009 |
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problem with PCR amplification of cDNAStarted by roshni mohan, 05 Aug 2017 PCR, Cloning, cDNA
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How to check if my primers were degraded?Started by Nephrite, 11 Aug 2017 |
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calculating ramp rate on thermocycler - urgentStarted by molbio1234, 12 Aug 2017 |
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Funny shaped PCR curveStarted by Vyse, 31 Jul 2017 |
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Different efficiency in GOI and HKStarted by Paja, 04 Jun 2017 No calibrator available and 1 more...
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How clean do I need my DNA to be?Started by seanspotatobusiness, 18 May 2017 |
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Problematic DNA ExtractionStarted by hmwmx, 14 Apr 2017 extraction, pcr, dna
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Length of amplicon for RT real time PCRStarted by Nephrite, 27 Mar 2017 |
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The curve of sample one is ideal or notStarted by Almoutazbellah Gamal, 09 Mar 2017 |
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Optimal dNTP storage pHStarted by Vyse, 24 Feb 2017 |
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REAL TIME PCR WITH TAQMAN PROBEStarted by ANUJ1706, 27 Feb 2017 |
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Tray stuck in ABI7300Started by Prissadee Thanaphongdecha, 23 Feb 2017 |
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