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PCR, RT-PCR and Real-Time PCR
Questions related to any PCR, primers etc.
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Reference gene(s) selection and validaton for qPCRStarted by Trof, 22 Apr 2014  housekeeping, normalization and 1 more...
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Inexplicable qPCR failure in single wellsStarted by Tabaluga, 18 Aug 2013 qPCR, real-time PCR, failure
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Real-Time RT-PCR one-step and two step issueStarted by Curtis, 26 Feb 2009 |
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What went wrong in my qPCR?Started by molecular_medicine, 19 Feb 2009 |
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How & where to prepare qPCRStarted by goyabean, 10 Aug 2009 |
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Primer dimer issue in real time PCRStarted by veer2, 21 Jun 2009 |
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PCR product purificationStarted by thegradstudent, 01 Apr 2004 |
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Smear in long distance PCRStarted by xabiarias, 02 Mar 2011 |
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PCR Contamination issuesStarted by Martorse, 01 Oct 2004 pcr, contamination
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Important literature for real time PCR usersStarted by tea-test, 29 Jan 2009 |
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How to get rid of PCR primer dimerStarted by bouttela, 17 Mar 2003 pcr, dimmer
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Understanding qPCR and gene expression changesStarted by Sciencefreak, 31 Mar 2009 |
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Normalization for RNA or cDNA during two step RT-PCR?Started by Curtis, 20 Apr 2009 |
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Optimization of real time-PCR - where should I start from?Started by Nephrite, 17 Apr 2018 |
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Weird PCR resultsStarted by Nephrite, 03 Apr 2018 |
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Primer design (Real time PCR)Started by Helder_90, 24 Mar 2018 |
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PCR is inconsistentStarted by waterwind, 04 Feb 2018 PCR
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Exact amplification in negative control???Started by astroboy89, 21 Mar 2018 |
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How to analyse Taqman results for Genotyping?Started by UCMann, 16 Mar 2018 |
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Specific primer problemStarted by Pipto, 28 Jan 2018 primer, PGP 9.5, RT-PCR
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How to minimize delta ct value when everything else worked well in qRT PCRStarted by Sciencerush, 03 Dec 2017 |
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How to minimize delta ct value when everything else worked well in qRT PCRStarted by Sciencerush, 04 Dec 2017 |
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Large Difference between Ct of 18s rRNA and Target GeneStarted by JonBio, 17 Feb 2010 |
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RAPD PCRStarted by Palitha, 16 Nov 2017 |
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 Answered
Instrument blocklower errorStarted by Kristaliukas, 05 Oct 2017 RT-PCR
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