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PCR, RT-PCR and Real-Time PCR
Questions related to any PCR, primers etc.
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Reference gene(s) selection and validaton for qPCRStarted by Trof, 22 Apr 2014  housekeeping, normalization and 1 more...
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Inexplicable qPCR failure in single wellsStarted by Tabaluga, 18 Aug 2013 qPCR, real-time PCR, failure
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Real-Time RT-PCR one-step and two step issueStarted by Curtis, 26 Feb 2009 |
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What went wrong in my qPCR?Started by molecular_medicine, 19 Feb 2009 |
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How & where to prepare qPCRStarted by goyabean, 10 Aug 2009 |
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Primer dimer issue in real time PCRStarted by veer2, 21 Jun 2009 |
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PCR product purificationStarted by thegradstudent, 01 Apr 2004 |
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Smear in long distance PCRStarted by xabiarias, 02 Mar 2011 |
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PCR Contamination issuesStarted by Martorse, 01 Oct 2004 pcr, contamination
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Important literature for real time PCR usersStarted by tea-test, 29 Jan 2009 |
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How to get rid of PCR primer dimerStarted by bouttela, 17 Mar 2003 pcr, dimmer
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Understanding qPCR and gene expression changesStarted by Sciencefreak, 31 Mar 2009 |
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Normalization for RNA or cDNA during two step RT-PCR?Started by Curtis, 20 Apr 2009 |
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ZEN probesStarted by Phoenix1978, 02 Nov 2021 probe, ZEN, qPCR
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different Ct values for RNaseP gene in human genomic DNAStarted by alex0727, 27 Jul 2021 |
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Erratic Cq values from PlasmidsStarted by Montys, 21 Apr 2021 plasmids, qPCR, Cq, stability
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Standard curve - copy number/ul and dilutionStarted by Thyago, 12 Jan 2021 viral quantification, rt-qpcr and 1 more...
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ABI 7900HT big alarmStarted by katoichi, 04 Nov 2020 qpcr, abi 7900ht
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Light genotyping PCR bandsStarted by Hummingbird2, 20 Aug 2020 |
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Re-PCR on a gel purified PCR product resulted in smear. Why?Started by Curtis, 27 Jul 2020 |
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What is the relation between amplicon size and melting temp?Started by Curtis, 23 May 2020 |
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Viral isolation kit QIA buffer substituteStarted by Trof, 07 Apr 2020 |
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viral RNA extraction kitStarted by SF_HK, 29 Dec 2019 PCR, DNA qPCR
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nested PCRStarted by SF_HK, 08 Dec 2019 amplicon length
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gain optimization closest match selectedStarted by nightingale, 17 Feb 2019 rotorgene Q
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