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PCR, RT-PCR and Real-Time PCR
Questions related to any PCR, primers etc.
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Reference gene(s) selection and validaton for qPCRStarted by Trof, 22 Apr 2014  housekeeping, normalization and 1 more...
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Inexplicable qPCR failure in single wellsStarted by Tabaluga, 18 Aug 2013 qPCR, real-time PCR, failure
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Real-Time RT-PCR one-step and two step issueStarted by Curtis, 26 Feb 2009 |
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What went wrong in my qPCR?Started by molecular_medicine, 19 Feb 2009 |
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How & where to prepare qPCRStarted by goyabean, 10 Aug 2009 |
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Primer dimer issue in real time PCRStarted by veer2, 21 Jun 2009 |
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PCR product purificationStarted by thegradstudent, 01 Apr 2004 |
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Smear in long distance PCRStarted by xabiarias, 02 Mar 2011 |
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PCR Contamination issuesStarted by Martorse, 01 Oct 2004 pcr, contamination
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Important literature for real time PCR usersStarted by tea-test, 29 Jan 2009 |
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How to get rid of PCR primer dimerStarted by bouttela, 17 Mar 2003 pcr, dimmer
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Understanding qPCR and gene expression changesStarted by Sciencefreak, 31 Mar 2009 |
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Normalization for RNA or cDNA during two step RT-PCR?Started by Curtis, 20 Apr 2009 |
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Re-PCR on a gel purified PCR product resulted in smear. Why?Started by Curtis, 27 Jul 2020 |
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What is the relation between amplicon size and melting temp?Started by Curtis, 23 May 2020 |
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Viral isolation kit QIA buffer substituteStarted by Trof, 07 Apr 2020 |
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viral RNA extraction kitStarted by SF_HK, 29 Dec 2019 PCR, DNA qPCR
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nested PCRStarted by SF_HK, 08 Dec 2019 amplicon length
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gain optimization closest match selectedStarted by nightingale, 17 Feb 2019 rotorgene Q
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Primer dilutionStarted by Mad Researcher, 20 May 2015 Primers, dilution, qPCR
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An odd situation: rfu values & scoring of AFLP fragments in clonally propagaStarted by alexander v., 06 Jan 2019 aflp, rfu, applied biosystems and 2 more...
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Efficiency curve of primersStarted by DionisioDD, 10 Jun 2019 qPCR, Pimers, Curve
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How do determine the reference gene for SMN 2Started by Mouna, 29 Mar 2019 q-RT-PCR, real time PCR and 1 more...
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qPCR inhibitionStarted by Lord Apoptosis, 19 Mar 2019 Real time PCR, PCR inhibition
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Dengue Virus SerotypingStarted by Faiz Ahmed Raza, 20 Feb 2019 human RNA contamination and 3 more...
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