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PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

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Topic	Stats	Last Post Info
Pinned Inexplicable qPCR failure in single wells Started by Tabaluga, 18 Aug 2013 qPCR, real-time PCR, failure	10 replies 5,513 views	Tabaluga 09 Oct 2014
Pinned Reference gene(s) selection and validaton for qPCR Started by Trof, 22 Apr 2014 housekeeping, normalization and 1 more...	2 replies 3,232 views	Trof 26 Jul 2014
Pinned Real-Time RT-PCR one-step and two step issue Started by Curtis, 26 Feb 2009	13 replies 15,517 views	Bassam27 10 Jun 2012
Pinned What went wrong in my qPCR? Started by molecular_medicine, 19 Feb 2009 1 2	Hot 27 replies 24,382 views	Curlis 29 May 2012
Pinned How & where to prepare qPCR Started by goyabean, 10 Aug 2009	9 replies 12,613 views	biznatch 29 May 2012
Pinned Primer dimer issue in real time PCR Started by veer2, 21 Jun 2009 1 2	Hot 21 replies 20,577 views	Curlis 21 May 2012
Pinned PCR product purification Started by thegradstudent, 01 Apr 2004 1 2 3 4	Hot 49 replies 42,239 views	Sunflower_gl 10 May 2012
Pinned Smear in long distance PCR Started by xabiarias, 02 Mar 2011 1 2 3	Hot 39 replies 27,215 views	chan8 13 Mar 2012
Pinned PCR Contamination issues Started by Martorse, 01 Oct 2004 pcr, contamination 1 2	Hot 27 replies 29,795 views	muntasir 21 Feb 2012
Pinned Important literature for real time PCR users Started by tea-test, 29 Jan 2009 1 2 3	Hot 41 replies 54,022 views	Ann_M 11 Jan 2012
Pinned How to get rid of PCR primer dimer Started by bouttela, 17 Mar 2003 pcr, dimmer 1 2 3	Hot 33 replies 45,062 views	cancerlab 13 Nov 2011
Pinned Understanding qPCR and gene expression changes Started by Sciencefreak, 31 Mar 2009	10 replies 15,249 views	genie 01 Jun 2011
Pinned Normalization for RNA or cDNA during two step RT-PCR? Started by Curtis, 20 Apr 2009 1 2	Hot 17 replies 23,513 views	Nephrite 28 Mar 2011
Can you use same samples for real time pcr 2nd time? Started by J092, Yesterday, 12:04 AM Real time pcr	0 replies 72 views	J092 Yesterday, 12:04 AM
Absolute quant with TaqMan - Different RT & different plates? Started by Amanda86, 22 Jan 2015	0 replies 90 views	Amanda86 22 Jan 2015
Staphylococcus aureus colony PCR Started by Raygoza, 21 Jan 2015	1 reply 165 views	CPRES 21 Jan 2015
The slightly bigger band dillema. Started by Tick, 16 Jan 2015 PCR, electrophoresis, gel and 2 more...	2 replies 283 views	Trof 17 Jan 2015
Could any of you help with an explanation to these vague real time PCR curves Started by Mohamed 1984, 26 Nov 2014	2 replies 447 views	Tick 16 Jan 2015
Non especific band in PCR... Started by lootches, 26 Dec 2014 PCR, ANEALING, TROUBLESHOOTING	2 replies 384 views	Tick 16 Jan 2015
PCR & Western Blot sample preperation Started by Homeostasis, 14 Jan 2015 PCR, qPCR, preparing and 2 more...	2 replies 213 views	Homeostasis 15 Jan 2015
Representing Ct values above set Cycle baseline? Started by KennMell, 10 Jan 2015 qRTPCR	1 reply 196 views	Trof 12 Jan 2015
I need help with PCR problems Started by Claritywind, 21 Dec 2014	5 replies 331 views	Claritywind 04 Jan 2015
Y-linker transgene integration site PCR primer verification Started by crysis405, 02 Jan 2015 PCR, primers, transgene	1 reply 214 views	phage434 02 Jan 2015
Capillary electrophoresis separation Started by Raygoza, 01 Jan 2015	0 views	Raygoza 01 Jan 2015
Question about baselines in Real-Time PCR Started by labbey2.0, 16 Dec 2014	3 replies 346 views	Trof 28 Dec 2014