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PCR, RT-PCR and Real-Time PCR
Questions related to any PCR, primers etc.
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Reference gene(s) selection and validaton for qPCRStarted by Trof, 22 Apr 2014  housekeeping, normalization and 1 more...
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Inexplicable qPCR failure in single wellsStarted by Tabaluga, 18 Aug 2013 qPCR, real-time PCR, failure
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Real-Time RT-PCR one-step and two step issueStarted by Curtis, 26 Feb 2009 |
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What went wrong in my qPCR?Started by molecular_medicine, 19 Feb 2009 |
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How & where to prepare qPCRStarted by goyabean, 10 Aug 2009 |
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Primer dimer issue in real time PCRStarted by veer2, 21 Jun 2009 |
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PCR product purificationStarted by thegradstudent, 01 Apr 2004 |
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Smear in long distance PCRStarted by xabiarias, 02 Mar 2011 |
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PCR Contamination issuesStarted by Martorse, 01 Oct 2004 pcr, contamination
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Important literature for real time PCR usersStarted by tea-test, 29 Jan 2009 |
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How to get rid of PCR primer dimerStarted by bouttela, 17 Mar 2003 pcr, dimmer
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Understanding qPCR and gene expression changesStarted by Sciencefreak, 31 Mar 2009 |
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Normalization for RNA or cDNA during two step RT-PCR?Started by Curtis, 20 Apr 2009 |
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Same primers and different melting peaks for two samples (~1°C appart)Started by sergio_pv, 22 Sep 2016 melting curve, melting peak
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Gene expression in colonic tissues using qRT-PCRStarted by Shima Bibi, 20 Sep 2016 RNA, Housekeeping gene and 1 more...
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qPCR run w. strong positive NTC signal but very low fluorescenceStarted by Paja, 13 Sep 2016 |
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Designing primers with his-tag for pET28a+ vectorStarted by Mashudu, 03 Sep 2016 pcr, cloning, molecular biology and 2 more...
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making your own qPCR kit!Started by nightingale, 24 Aug 2016 any experiences ?
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Problem with TaqMan qPCR efficiencyStarted by Emilie, 14 Aug 2016 qPCR, TaqMan, real-time PCR and 2 more...
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Strange Taqman probe negative fluorescenceStarted by Rodriguez Ruslan, 01 Apr 2016 real-time PCR, taqman and 2 more...
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qPCR data analysisStarted by Tarique, 10 Jan 2016 qPCR, Real-time PCR, PCR and 1 more...
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PCR contaminationStarted by Gizele Luz, 26 Dec 2015 |
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-RT contamination problem in PCRStarted by ashjlss, 19 Apr 2016 PCR, contamination
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testing primers efficiency for rt-PCRStarted by madelingirly, 27 Apr 2016 primer, rt-pcr
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High molecular weight band from my PCRStarted by dexturelab, 25 May 2016 |
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