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Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

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Topic	Stats	Last Post Info
glycerol stocks Started by arnabde2000, 24 Oct 2009	10 replies 3,068 views	stock-man 11 Dec 2009
Extremely small bacterial colonies after transformation Started by Agnes85, 28 Apr 2010	10 replies 1,954 views	Agnes85 23 May 2010
please help!!! with transformation Started by cusycon, 04 Nov 2009	10 replies 2,975 views	christy 11 Dec 2009
How to inoculate bacteria? Started by dakims, 11 Nov 2009	10 replies 3,290 views	jiajia1987 12 Nov 2009
3 way ligation help needed Started by Stuck with Ligation, 13 Nov 2009	10 replies 2,432 views	Stuck with Ligation 18 Nov 2009
dose incubation in 16 C increase the ligation efficiebcy? Started by mahsa, 11 Sep 2010	10 replies 1,125 views	mahsa 15 Sep 2010
Problems with qiaquick gel purification kit Started by losybelle, 01 Dec 2010	10 replies 1,606 views	losybelle 28 Jun 2011
ligation--pls help! Started by janani, 05 Jan 2011	10 replies 1,862 views	TheBalrog 10 Mar 2011
Loss of protein expression following double transformation Started by WormsPhD, 12 Feb 2011	10 replies 1,371 views	WormsPhD 03 Mar 2011
No bands on agarose gel after restriction digestion. Started by libra84, 05 Apr 2011	10 replies 1,741 views	Shiny 19 Apr 2011
screening for GPF Started by Tracy0110, 22 May 2012 GFP, pGreen, vector	10 replies 634 views	phage434 04 Jun 2012
Weird Library Transformation Results Started by Christos Karamitros Oyama, 10 Aug 2012	10 replies 424 views	Christos Karamitros Oyama 11 Aug 2012
E. coli growing on plates and bot not in LB medium Started by nescardoso, 06 Sep 2012 bacteria, E. coli, kanamycin, LB and 1 more...	10 replies 548 views	phage434 07 Sep 2012
Colony PCR and digestion with KpnI Started by prabhubct, 11 Sep 2012 pcr, Restriction digestion	10 replies 453 views	prabhubct 12 Sep 2012
Co-transfection with 2 plasmids Started by science noob, 19 Sep 2012	10 replies 528 views	Trof 21 Sep 2012
Amplicon as Template in PCR for TOPO TA Cloning Started by Epigeneticist, 01 Dec 2012 PCR, TOPO TA CLONING, CLONING	10 replies 278 views	Trof 01 Dec 2012
High frequency of vector religation Started by HBImolbiol, 11 Feb 2013	10 replies 191 views	HBImolbiol 12 Feb 2013
Long PCR product Started by Kami23, 27 Jan 2009	9 replies 3,843 views	Kami23 30 Jan 2009
Problem cloning oligo into double digested vector Started by nIUro, 17 Feb 2009	9 replies 4,458 views	phage434 20 Feb 2009
Transformation question Started by Ecoli0157, 19 Feb 2009	9 replies 4,228 views	HomeBrew 20 Feb 2009
ligation question Started by yoda1311, 21 Mar 2009	9 replies 2,946 views	phage434 22 Mar 2009
an annoying cloning Started by Biogareth, 02 Apr 2009	9 replies 3,332 views	Biogareth 09 Apr 2009
Ligation problem Started by sktewarybt1993, 13 Apr 2009	9 replies 3,470 views	eldon 24 May 2009
No colony for oligo cloning Started by Zhongbo, 13 Jun 2009	9 replies 2,929 views	Zhongbo 18 Jun 2009
Transformant plating advise Started by LordPhantom, 18 Jul 2009	9 replies 2,920 views	LordPhantom 22 Jul 2009