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Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

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Topic	Stats	Last Post Info
Cloned but not expressing! Started by mansi368, 11 Feb 2013	8 replies 261 views	GNANA 26 Feb 2013
10,000 bp size band showing up in gel of plasmid extraction Started by tihong10, 25 Feb 2013 gDNA contamination and 2 more...	1 reply 136 views	Curtis 25 Feb 2013
Transformant RFP expression Started by tihong10, 25 Feb 2013 RFP, DH5a, Transformation	0 replies 145 views	tihong10 25 Feb 2013
cDNA amplification problem Started by thenotoriousROC, 21 Feb 2013	4 replies 197 views	thenotoriousROC 22 Feb 2013
Confirmation of ligation Started by neuron, 18 Feb 2013 PCR RD	7 replies 213 views	jerryshelly1 21 Feb 2013
Unexpected base after deletion mutagenesis Started by crispcrimp, 20 Feb 2013 PCR, ligase, mutagenesis	3 replies 155 views	GNANA 21 Feb 2013
Ligation of large ammount of insert (no transformation) Started by Eddie*, 18 Feb 2013 ligation	7 replies 219 views	Eddie* 21 Feb 2013
Ligation: two basic questions Started by Spright, 21 Feb 2013	1 reply 132 views	GNANA 21 Feb 2013
5'end of pcr product was degraded after ligation and transformation Started by Awnman, 18 Feb 2013 cloning	5 replies 190 views	neuron 20 Feb 2013
Allelic Exchange in Pseudomonas aeruginosa Started by gandhi, 20 Feb 2013 Pseudomonas aeruginosa and 4 more...	0 replies 124 views	gandhi 20 Feb 2013
PCR and restriction enzyme digestion Started by ahmadfathi, 16 Feb 2013	3 replies 202 views	bob1 17 Feb 2013
Problems with designing an experiment in identifying mutations of particular gen Started by helpwithdna, 14 Feb 2013	5 replies 187 views	bob1 16 Feb 2013
Probelm with double digestion Started by shaajahan7, 15 Feb 2013 Probelm with double dige	1 reply 163 views	cellthetruth 15 Feb 2013
Cloning not working, all steps checked, clueless Started by LenaNS, 15 Feb 2013 Cloning, Impact, SapI, Ligation and 1 more...	1 reply 205 views	phage434 15 Feb 2013
recombinant protein form Started by Gunalan, 14 Feb 2013 inclusion body or secret	2 replies 490 views	neuron 14 Feb 2013
Cloning problem, sticky - blunt ends ligation fail . Started by Danbas28, 12 Feb 2013 blunt ends ligation and 2 more...	4 replies 196 views	Danbas28 13 Feb 2013
restricted PCR plasmid runs slower Started by Wickerl3732, 12 Feb 2013 cloning, restriction, agarose gel	2 replies 166 views	Wickerl3732 13 Feb 2013
High frequency of vector religation Started by HBImolbiol, 11 Feb 2013	10 replies 188 views	HBImolbiol 12 Feb 2013
DNA ligation and trasformation Started by ahmadfathi, 06 Feb 2013 Cloning	6 replies 291 views	ahmadfathi 11 Feb 2013
Is it possible to induce signalling pathways activity in Hela cells using multip Started by Ivanaaa, 10 Feb 2013 cancer, cloning, luciferase assay and 2 more...	1 reply 214 views	bob1 10 Feb 2013
What is a fos-pomoter and what does SV40 late pA do? Started by Joanna_Andersen, 10 Feb 2013 cloning, promoter, vector	0 replies 180 views	Joanna_Andersen 10 Feb 2013
Stuck from last august! need help urgently - cloning and pcr ! Started by mansi368, 05 Feb 2013	7 replies 218 views	bob1 06 Feb 2013
QIAGEN Spin Columns and Minipreps - Alternatives?! Started by Luria Bertani, 14 Aug 2012	13 replies 640 views	Lisa Hsu 06 Feb 2013
How to set up simultaneous digestion? Started by Guf, 01 Feb 2013 plasmid, vector, digestion	3 replies 247 views	phage434 04 Feb 2013
I have a 96 tandem array repeat clone but need half of them in new clone Started by nvramakanth, 22 Jan 2013	4 replies 165 views	phage434 04 Feb 2013