Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

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Topic	Stats	Last Post Info
No bands on agarose gel after restriction digestion. Started by libra84, 05 Apr 2011	10 replies 1,128 views	Shiny 19 Apr 2011
knockdown: yes; GFP: no ?! what is this? Started by Ytje, 07 Apr 2011	2 replies 548 views	Ytje 19 Apr 2011
pSIM in BL21(DE3) Started by vinu23pari, 17 Apr 2011	2 replies 657 views	pDNA 18 Apr 2011
Help! Problems with SDM with QUikChange II XL site-directed mutagenesis kit Started by eyeboy, 14 Apr 2011	2 replies 741 views	jaya2020 18 Apr 2011
RE digestion Started by grvsomani, 14 Apr 2011	4 replies 889 views	Shiny 17 Apr 2011
pLys plasmid containing bacterial strains Started by Luria Bertani, 16 Apr 2011	1 reply 644 views	pDNA 16 Apr 2011
Weird problem in invitromutagenesis Started by jaya2020, 16 Apr 2011	3 replies 623 views	pDNA 16 Apr 2011
problem with cloning p16ink4a into pEGFP-C2 Started by kijitengu, 12 Apr 2011	1 reply 576 views	bob1 14 Apr 2011
HELP!!! Started by billi, 13 Apr 2011	3 replies 623 views	phage434 14 Apr 2011
preparation of LB/ampicillin/IPTG/X-gal plate Started by jamestoon, 08 Apr 2011	3 replies 717 views	perneseblue 12 Apr 2011
Please, I need GFP vector Started by mrgawad, 12 Apr 2011	0 replies 430 views	mrgawad 12 Apr 2011
Cloning of ITS region Started by lizrene, 10 Apr 2011	6 replies 595 views	lizrene 11 Apr 2011
Low yield by mini prep on lentivector system Started by Stephen ahn, 07 Apr 2011	0 replies 413 views	Stephen ahn 07 Apr 2011
SmaI blunt end ligation problem... Started by gauravmdgl, 05 Apr 2011	3 replies 564 views	gauravmdgl 06 Apr 2011
inverse pcr Started by deespike, 03 Apr 2011	2 replies 763 views	deespike 06 Apr 2011
Viral cDNA Library Started by Optimistic, 02 Apr 2011	3 replies 619 views	bob1 04 Apr 2011
How can i amplify four subclasses igg from PBMC? Started by JM109, 04 Apr 2011	0 replies 427 views	JM109 04 Apr 2011
Chemical competent E.coli always from single colony ? Started by pET, 22 Mar 2011	2 replies 653 views	perneseblue 03 Apr 2011
invitro mutagenesis using stragene kit Started by jaya2020, 28 Mar 2011	4 replies 756 views	jaya2020 30 Mar 2011
metylated cloning vector Started by oms, 29 Mar 2011	2 replies 571 views	oms 30 Mar 2011
Seeing two SDS-PAGE bands upon protein expression from pET-30b(+) Started by Zaineb, 29 Mar 2011	1 reply 625 views	protolder 29 Mar 2011
Overexpression of mouse constructs in human cell lines Started by Rubicon, 23 Mar 2011	6 replies 603 views	Rsm 28 Mar 2011
Secretion cloning vectors Started by pDNA, 28 Mar 2011	1 reply 563 views	protolder 28 Mar 2011
complementing a double knockout in E. coli Started by nathankho, 25 Mar 2011	1 reply 612 views	pDNA 25 Mar 2011
dephosphorilation of vector ends Started by eyes, 24 Mar 2011	3 replies 685 views	eyes 25 Mar 2011

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Molecular Cloning

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