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Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

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Topic	Stats	Last Post Info
Cloning purified PCR products eluted in Qiagen's EB Started by kmkocot, 31 Jan 2009	11 replies 5,295 views	little mouse 23 Feb 2009
Reusing LB/amp/xgal plates (also, how long can e. coli be in the incubator for?) Started by Soda, 11 Jul 2009	4 replies 4,965 views	leelee 13 Jul 2009
cloning trouble Started by crackanaut, 17 Mar 2009 1 2	Hot 15 replies 4,944 views	jiajia1987 24 Mar 2009
What does the DH5alpha mean in E.coli DH5alpha bacteria Started by minemin, 26 Apr 2009	10 replies 4,918 views	Ratna 15 Dec 2009
cloning help Started by yesandno, 09 Aug 2009	9 replies 4,893 views	yesandno 21 Aug 2009
Ligation trouble continues Started by hester, 05 Aug 2009	9 replies 4,856 views	phage434 11 Aug 2009
Low sized bands in plasmid preperations Started by ram, 13 May 2009	13 replies 4,793 views	Vini 21 Jul 2009
cloning 1052bp fragment Started by SF_HK, 10 Feb 2009	11 replies 4,671 views	scolix 21 Feb 2009
Determine annealing temperature of primers Started by minty, 26 Apr 2009	Hot 14 replies 4,611 views	minty 11 May 2009
Stored bacteria lost plasmids? Started by KatieB, 06 Feb 2009	12 replies 4,471 views	Qundo12 25 Feb 2009
Help for T-A cloning Started by shashababy, 19 Jun 2009 1 2	Hot 15 replies 4,461 views	novagen 23 Jul 2009
Problem cloning oligo into double digested vector Started by nIUro, 17 Feb 2009	9 replies 4,458 views	phage434 20 Feb 2009
Gel electrophoresis and purifying dna Started by lyok, 22 Aug 2009	6 replies 4,383 views	Warren 07 Sep 2009
[vote] The best Gel extraction kit Started by lactamase, 07 Aug 2009	11 replies 4,325 views	Ivanov_br 16 Aug 2009
Ligation very large vector and small insert Started by Pancho, 29 Jan 2009	7 replies 4,257 views	scolix 28 Feb 2009
making competent E coli DH5a cells Started by minemin, 30 Jan 2009	6 replies 4,257 views	perneseblue 31 Jan 2009
Cloning strat...Awful or ok? Started by uncutlateralus, 28 Jul 2009	10 replies 4,232 views	perneseblue 30 Jul 2009
Transformation question Started by Ecoli0157, 19 Feb 2009	9 replies 4,229 views	HomeBrew 20 Feb 2009
Is my insert present??? Started by rocketfan86, 28 Oct 2009 1 2	Hot 17 replies 4,214 views	HomeBrew 30 Oct 2009
My battle with BamHI Started by georgiadave, 23 Mar 2010 1 2	Hot 27 replies 4,185 views	georgiadave 02 Apr 2010
Purifying and cloning 69nt product Started by umam, 28 Jul 2009	5 replies 4,162 views	Penguin 31 Jul 2009
Storage of invitrogen top10 cells Started by epigenetics, 23 Jun 2009	11 replies 4,016 views	ram 08 Jul 2009
ligation problem Started by sagar, 09 Mar 2009	11 replies 4,013 views	sagar 12 Mar 2009
Restriction Enzyme Digest of Genomic DNA Started by Substance, 22 Jul 2009	9 replies 3,973 views	HomeBrew 26 Jul 2009
No band in the gel after ligation Started by priyak, 17 Mar 2009	6 replies 3,931 views	Nrelo 26 Mar 2009