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Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

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Topic	Stats	Last Post Info
TA cloning: ligation problem or toxic construct? Started by DrLeo, 23 Apr 2013 TA cloning	4 replies 156 views	DrLeo 24 Apr 2013
Do I need to sequence after digestion? Started by smndtupidisaftr, 22 Apr 2013	9 replies 187 views	pito 24 Apr 2013
Reappearing band shifts/smears after double digest and gel purification Started by muLab, 23 Apr 2013 restriction, smear, NotI, SbfI and 1 more...	2 replies 160 views	muLab 23 Apr 2013
Problem with double digestion: one enzyme is not working Started by Sandy143, 15 Apr 2013 BamHI, HindII, Double digestion	8 replies 277 views	Sandy143 21 Apr 2013
difficulty to clone and/or express proteases. Toxicity? Started by monicalvi, 19 Apr 2013 protease, dh5 alpha, induction and 1 more...	0 replies 200 views	monicalvi 19 Apr 2013
Low copy vector for library generation Started by pDNA, 10 Apr 2013	2 replies 163 views	pDNA 19 Apr 2013
can you recommend a gateway mammalian expression vector? Please? :) Started by leelee, 12 Jun 2012	1 reply 432 views	leelee 18 Apr 2013
Problems with crossover PCR Started by shawn6022, 15 Apr 2013 crossover PCR	2 replies 131 views	shawn6022 15 Apr 2013
problems with plasmid miniprep Started by shawn6022, 08 Apr 2013 plasmid prep	8 replies 266 views	shawn6022 15 Apr 2013
gateway destination vector grows on kanamycin plates !!!!! Started by Opal, 13 Apr 2013	1 reply 136 views	isamila 15 Apr 2013
Two genes, same MCS Started by Labmouse9, 13 Apr 2013 cloning	1 reply 168 views	bob1 13 Apr 2013
Tet-Express Inducible Expression System Started by protein2, 13 Apr 2013	0 replies 146 views	protein2 13 Apr 2013
Plasmid Extraction from P. Aeruginosa smears in Gel! Started by tihong10, 11 Apr 2013 plasmid extraction, pa14, smear and 1 more...	1 reply 145 views	pito 12 Apr 2013
pkp 59 vector Started by blaszcz, 07 Mar 2013 any info	2 replies 129 views	blaszcz 12 Apr 2013
Including ATG in cloning? Started by Photoreceptor, 11 Apr 2013 Cloning, inframe, pCAG-EGFP	1 reply 137 views	bob1 11 Apr 2013
Can't find a recombinant after ligating Started by Ahrenhase, 09 Apr 2013	4 replies 149 views	Ahrenhase 09 Apr 2013
gene cloning Started by Rampa, 07 Apr 2013	1 reply 178 views	bigudukaz 07 Apr 2013
problem with ligation of linker/adaptor Started by Always_student, 04 Apr 2013 linker/adaptor ligation	2 replies 180 views	Always_student 07 Apr 2013
Is ligation really necessary? Started by Ahrenhase, 05 Apr 2013	6 replies 208 views	Ahrenhase 06 Apr 2013
Understand the basics of molecular cloning Started by wayfind, 21 Mar 2013	6 replies 248 views	cellthetruth 04 Apr 2013
Mutation in cloning Started by DrLeo, 25 Mar 2013	6 replies 215 views	bob1 02 Apr 2013
Ligation troubleshooting! Please help! Started by Binderya, 31 Mar 2013	5 replies 236 views	Binderya 02 Apr 2013
GFP -weak signal -why? Started by Louise Von Gersdorff Jørg, 01 Apr 2013 GFP, weak signal, cloning	1 reply 205 views	bob1 02 Apr 2013
Query regarding primers for quick change mutagenesis Started by kartiga natarajan, 26 Mar 2013 Problem in primers	3 replies 256 views	HOYAJM 01 Apr 2013
Truncated gene Cloning Started by Mariam, 29 Mar 2013	1 reply 261 views	phage434 30 Mar 2013