Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

Moderators wanted

BioForum needs more volunteer moderators to provide expert help to other members by participating in forum discussions and answering questions. If you are interested, please follow this link to apply.

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

Please log in to post a topic
Mark this forum as read

Topic	Stats	Last Post Info
Two genes, same MCS Started by Labmouse9, 13 Apr 2013 cloning	1 reply 165 views	bob1 13 Apr 2013
Colony-PCR workaround Started by Spright, 22 Jan 2013	3 replies 163 views	Trof 24 Jan 2013
Probelm with double digestion Started by shaajahan7, 15 Feb 2013 Probelm with double dige	1 reply 163 views	cellthetruth 15 Feb 2013
Need urgent advise.. Started by M.T2013, 29 Apr 2013 cloning	3 replies 162 views	JYaron 13 May 2013
Low copy vector for library generation Started by pDNA, 10 Apr 2013	2 replies 161 views	pDNA 19 Apr 2013
cloning antisense promoter in pGL3Basic Started by chonela, 22 Oct 2012	2 replies 157 views	chonela 22 Oct 2012
Problem with 3 way ligation Started by Ahrenhase, 27 Mar 2013	2 replies 156 views	Ahrenhase 28 Mar 2013
Maintainence of transformed cells Started by criscastells, 14 Mar 2013	5 replies 155 views	bob1 14 Mar 2013
Unexpected base after deletion mutagenesis Started by crispcrimp, 20 Feb 2013 PCR, ligase, mutagenesis	3 replies 154 views	GNANA 21 Feb 2013
Reappearing band shifts/smears after double digest and gel purification Started by muLab, 23 Apr 2013 restriction, smear, NotI, SbfI and 1 more...	2 replies 154 views	muLab 23 Apr 2013
Cloning Mitochondrial Targeting Sequence Started by speci, 27 Apr 2013	4 replies 154 views	speci 29 Apr 2013
polymerase to use for cloning Started by srpres, 06 Mar 2013 cloning polymerase	4 replies 150 views	bob1 06 Mar 2013
After ligation,I found that my colony on amplicilin plate have not insert gene Started by sagebook, 17 Jan 2013 ligation digestion	1 reply 149 views	bob1 17 Jan 2013
Problems regarding point mutations.... Started by kartiga natarajan, 08 Mar 2013 Trouble in PCR	4 replies 149 views	kartiga natarajan 10 Mar 2013
Can't find a recombinant after ligating Started by Ahrenhase, 09 Apr 2013	4 replies 148 views	Ahrenhase 09 Apr 2013
Problems with growing out transformants in liquid LB Started by Toastayy, 09 Jan 2013	3 replies 147 views	Toastayy 09 Jan 2013
Alternative to pET Vectorsystem Started by Pangea, 13 Jan 2013 Vectors	2 replies 146 views	Pangea 14 Jan 2013
Issue with Xba1 and Dam methylation Started by Ahrenhase, 26 Mar 2013	7 replies 146 views	phage434 28 Mar 2013
Transformant RFP expression Started by tihong10, 25 Feb 2013 RFP, DH5a, Transformation	0 replies 145 views	tihong10 25 Feb 2013
sequencing problems Started by lenkabo, 22 Mar 2013	2 replies 144 views	lenkabo 22 Mar 2013
Tet-Express Inducible Expression System Started by protein2, 13 Apr 2013	0 replies 144 views	protein2 13 Apr 2013
Plasmid Extraction from P. Aeruginosa smears in Gel! Started by tihong10, 11 Apr 2013 plasmid extraction, pa14, smear and 1 more...	1 reply 142 views	pito 12 Apr 2013
TA cloning: ligation problem or toxic construct? Started by DrLeo, 23 Apr 2013 TA cloning	4 replies 142 views	DrLeo 24 Apr 2013
Smear after digestion of a plasmid from a Dam- strain Started by Schevchen, 05 Nov 2012	1 reply 141 views	phage434 05 Nov 2012
Problems with second recombination in mutagenesis with suicide vector Started by Fuza, 17 Oct 2012 mutgenesis, suicide vector and 3 more...	0 replies 140 views	Fuza 17 Oct 2012