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Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

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Topic	Stats	Last Post Info
Annealing Oligos - Annealing Buffer vs TE vs Water Started by Francisco, 20 Jan 2011	0 replies 759 views	Francisco 20 Jan 2011
Microporation Started by andyg2886, 14 Jan 2011	0 replies 887 views	andyg2886 14 Jan 2011
Recs for a lentiviral shRNA expression vector with RFP? Started by yoursisterdebra, 18 Jan 2011	0 replies 600 views	yoursisterdebra 18 Jan 2011
Improving transformation efficiency in Streptococcus pneumoniae Started by SJB, 20 Jan 2011	0 replies 584 views	SJB 20 Jan 2011
GFP tag into target gene containing vector or target gene into GFP containing ve Started by killadi, 28 Jan 2011	0 replies 671 views	killadi 28 Jan 2011
Preparation of competent Listeria monocytogenes cells for heat shock tranformati Started by lab_microbe, 31 Jan 2011	0 replies 844 views	lab_microbe 31 Jan 2011
vector for promoter studies Started by eyes, 15 Feb 2011	0 replies 594 views	eyes 15 Feb 2011
L. lactis transformation Started by Dr. Desai, 17 Feb 2011	0 replies 791 views	Dr. Desai 17 Feb 2011
yeast transformation mistake (?) Started by 3hugger, 19 Feb 2011	0 replies 794 views	3hugger 19 Feb 2011
the shelf life of PAXgene RNA blood tubes? Started by jianbo, 23 Feb 2011	0 replies 675 views	jianbo 23 Feb 2011
Is there anybody have the sequence of pRARE plasmid? Started by Qundo12, 06 Mar 2011	0 replies 1,326 views	Qundo12 06 Mar 2011
Transformation of a genomic library - colony screening Started by cyril, 07 Mar 2011	0 replies 703 views	cyril 07 Mar 2011
weird restriction pattern after cloning Started by Opal, 16 Mar 2011	0 replies 692 views	Opal 16 Mar 2011
expression in TopoTA but not in mscv Started by clarer, 17 Mar 2011	0 replies 718 views	clarer 17 Mar 2011
Nested DNA deletions - any experience? Started by angelawu, 22 Mar 2011	0 replies 717 views	angelawu 22 Mar 2011
Sequecing TA clones often results in different qualities Started by phucvn, 23 Mar 2011	0 replies 623 views	phucvn 23 Mar 2011
How can i amplify four subclasses igg from PBMC? Started by JM109, 04 Apr 2011	0 replies 650 views	JM109 04 Apr 2011
Low yield by mini prep on lentivector system Started by Stephen ahn, 07 Apr 2011	0 replies 661 views	Stephen ahn 07 Apr 2011
Please, I need GFP vector Started by mrgawad, 12 Apr 2011	0 replies 658 views	mrgawad 12 Apr 2011
Cloning 3.7kb blunt PCR fragment in 12kb blunt vector Started by hematopoietry, 27 Apr 2011	0 replies 805 views	hematopoietry 27 Apr 2011
pCGN plasmid Started by sandy24, 29 Apr 2011	0 replies 922 views	sandy24 29 Apr 2011
HA tag construct Started by george005, 09 May 2011	0 replies 548 views	george005 09 May 2011
pacyc184 vector Started by snehaSK, 12 May 2011	0 replies 732 views	snehaSK 12 May 2011
Ligation Trouble Shooting Started by RMClone, 13 May 2011	0 replies 620 views	RMClone 13 May 2011
quantifying and sequencing BAC Started by krystle, 24 May 2011	0 replies 719 views	krystle 24 May 2011