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Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

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Topic	Stats	Last Post Info
No desired insert found in colonies Started by lily5658, 24 Apr 2012 Topo, colonies insert	0 replies 332 views	lily5658 24 Apr 2012
Ligation into a BAC? Started by Gnatnog, 31 Jul 2012	2 replies 331 views	phage434 07 Aug 2012
Three way ligation problem Started by bispecific antibody, 12 Sep 2012	6 replies 330 views	Curtis 12 Sep 2012
for T4 poly filling reaction Started by bluetutu2010, 11 Dec 2010	0 replies 329 views	bluetutu2010 11 Dec 2010
transformation of vector into DH10Bac competent cells Started by lufei, 13 Jul 2012	0 replies 329 views	lufei 13 Jul 2012
molecular cloning Started by Rampa, 11 Dec 2012 puc19	7 replies 329 views	phage434 23 Dec 2012
cloning to pET28a Started by osnattirosh, 13 Aug 2012	1 reply 328 views	ascacioc 13 Aug 2012
digested plasmid Started by biotech10, 02 Sep 2012	2 replies 327 views	phage434 03 Sep 2012
Problem with cloning - what is wrong? Started by Guf, 04 Jan 2013 Cloning, dephopshorylation and 2 more...	6 replies 321 views	phage434 09 Jan 2013
cloning a gene in to a plasmid Started by jameela, 08 Oct 2012	5 replies 319 views	jameela 25 Oct 2012
Difference between types of bacterial culture broths? Started by science noob, 19 Sep 2012	4 replies 311 views	bob1 23 Sep 2012
TOPO TA CLONING - MOLAR RATIO Started by Epigeneticist, 15 Dec 2012 TOPO TA CLONING and 2 more...	2 replies 310 views	Epigeneticist 15 Dec 2012
About gateway cDNA library Started by dysbindin, 26 Jul 2012	0 replies 309 views	dysbindin 26 Jul 2012
vector dephosphorylation Started by gulash, 11 Oct 2012 cloning, dephosphorylation	5 replies 308 views	phage434 11 Oct 2012
Smear in plasmid sample Started by science noob, 14 Oct 2012	9 replies 307 views	gyma 16 Oct 2012
mamallian expression vector that has FLAG tag and GFP Started by rock&science, 01 Aug 2012	3 replies 306 views	Curtis 01 Aug 2012
colony cracking for screening of positive clones Started by helena kyle, 16 Oct 2012 colony cracking, cloning and 1 more...	2 replies 305 views	helena kyle 30 Oct 2012
identification of target sequence Started by helpwithdna, 16 Dec 2012	3 replies 303 views	bob1 17 Dec 2012
Cloning Problem! I need help Started by RaquelCB86, 12 Oct 2012 pGem Vector, PCR colony and 1 more...	3 replies 302 views	phage434 14 Oct 2012
No insert in subcloning Started by gulash, 10 Nov 2012 cloning, insert	3 replies 302 views	gulash 14 Nov 2012
Gateway technology. Started by munis, 29 Sep 2012	4 replies 294 views	Trof 30 Sep 2012
TOPO TA Cloning Started by Epigeneticist, 10 Nov 2012 CLONING, TOPO TA and 2 more...	1 reply 293 views	phage434 10 Nov 2012
DNA ligation and trasformation Started by ahmadfathi, 06 Feb 2013 Cloning	6 replies 293 views	ahmadfathi 11 Feb 2013
Use RT-PCR to verify after transient transfection a plasmid? Started by kblee, 13 Sep 2012 transent transfection	5 replies 291 views	pcrman 13 Sep 2012
protein is not expessing in HEK 293 cells Started by Ashish Saroha, 08 Oct 2012 molecular cloning	2 replies 291 views	gyma 16 Oct 2012