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Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

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Topic	Stats	Last Post Info
NZY+Broth can be replaced by LB Broth in mutagenesis exp Started by shoaibarif, 03 May 2012	2 replies 396 views	hln 05 May 2012
Tagging a chromosomal gene Started by Nathaniel Saxe, 25 Apr 2012 gfp, fusion, protein	0 replies 395 views	Nathaniel Saxe 25 Apr 2012
Beta-mercaptoethanol in transformation process? Started by science noob, 07 Dec 2011	0 replies 392 views	science noob 07 Dec 2011
No bands at all after double digestion Started by hez2, 24 Jul 2012	1 reply 392 views	gyma 24 Jul 2012
Clontech genome walker expression Started by NTTH, 12 Jan 2012	0 replies 391 views	NTTH 12 Jan 2012
plasmid tetR and tet promoter Started by pDNA, 26 Jul 2012	2 replies 391 views	phage434 12 Sep 2012
should I do blunt end my pcr product before ligation Started by gulsatar, 07 Jun 2012 ligation pcr	2 replies 390 views	Kamran 08 Jun 2012
Cannot see insert with colony pcr Started by Smog187, 02 Dec 2012 Colony PCR, Cloning	6 replies 390 views	bob1 04 Dec 2012
pCMV-CD19 vector Started by Manish Neupane, 29 Nov 2010	0 replies 389 views	Manish Neupane 29 Nov 2010
SURE E. coli competent cells Started by annulik, 07 Sep 2012 SURE, E.COLI, COMPETENT CELLS and 1 more...	1 reply 386 views	prabhubct 07 Sep 2012
pSEX81 Started by finch.princess, 16 Feb 2012 phagemid, hyperphage	0 replies 384 views	finch.princess 16 Feb 2012
Screening a mutant amplified library Started by Makosad, 29 Feb 2012	0 replies 383 views	Makosad 29 Feb 2012
Creating a stable cell line by transfecting a plasmid without any fluorescence t Started by science noob, 20 Aug 2012	3 replies 382 views	bob1 20 Sep 2012
SAP activity after expiring date Started by lele987, 24 Nov 2011	0 replies 378 views	lele987 24 Nov 2011
Dam-/+ e. coli strains Started by Khudson0805, 16 Aug 2012	6 replies 378 views	Khudson0805 22 Aug 2012
Plzzz help .....primers ! Started by yusii, 02 May 2012 primer design, RBS	0 replies 377 views	yusii 02 May 2012
Vector Digestion Problem Started by kariskalon, 18 Sep 2012	4 replies 377 views	bob1 23 Sep 2012
pET32a size limitations Started by biology_06er, 16 Jun 2012	0 replies 376 views	biology_06er 16 Jun 2012
Cloning & Expression Problem in HEK-293Ts Started by IXA, 24 Sep 2012 Cloning, Expression, Myc, HEK-293 and 1 more...	5 replies 376 views	bob1 25 Sep 2012
Cloning genetic sequences of proteins. Started by alexifrank, 20 Jun 2012	1 reply 375 views	Inmost sun 21 Jun 2012
How to insert genes into chromosome of E. coli Started by donny, 25 Apr 2012	1 reply 373 views	donny 26 Apr 2012
Maximum insert size for EBNA-1 based episomal vector Started by neuropath, 19 Jan 2012	0 replies 370 views	neuropath 19 Jan 2012
Directions of genes expression illustrations Started by Akila Wijerathna Yapa, 12 Nov 2012 cloning, vectors, transposons and 1 more...	5 replies 370 views	Akila Wijerathna Yapa 14 Nov 2012
Plasmid preps of pETDuet-1 constructs Started by biolcrazy, 01 May 2012	0 replies 369 views	biolcrazy 01 May 2012
Is the genomic DNA similar among different cell lines? Started by gyma, 12 Jul 2012	4 replies 369 views	gyma 16 Jul 2012