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Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

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Topic	Stats	Last Post Info
Pinned Smear in my restriction digest Started by panda, 07 May 2009	12 replies 9,777 views	wincel 24 Sep 2010
Pinned How to transfect two plasmids in a cell Started by muchmoa, 04 May 2009	10 replies 9,194 views	laurequillo 25 Sep 2009
Pinned Problems with Stratagene Site Directed Mutagenesis Started by yean_ny_nie, 07 Apr 2009 1 2 3	Hot 36 replies 23,305 views	fighter 25 May 2012
Pinned Creating empty vector by self-ligation Started by chijing, 06 Apr 2009 1 2 3	Hot 31 replies 26,578 views	kellyhayes 23 Nov 2009
Pinned ds-oligo cloning/ligation issues Started by GilsonGirl, 06 Mar 2009 1 2	Hot 18 replies 8,490 views	Trof 03 Apr 2012
Pinned Help with 3-way ligation Started by PhilS, 24 Feb 2009	11 replies 10,013 views	PhilS 15 Mar 2009
Pinned Sequential restriction digestion of plasmid Started by shimshady, 21 Feb 2009 1 2 3 4	Hot 58 replies 33,721 views	mahrak 23 Jun 2012
Pinned What is the biggest insert for a viral vector? Started by Kami23, 06 Feb 2009 1 2	Hot 15 replies 12,124 views	wincel 24 Sep 2010
Pinned Old threads on Molecular Cloning are achived here Started by bioforum, 03 Feb 2009	0 replies 5,038 views	bioforum 03 Feb 2009
Pinned Tips on ethanol wash after nucleic acid precipitation Started by mario2004, 13 Mar 2005 1 2	Hot 22 replies 20,140 views	Maddie 30 Jul 2010
Pinned Time needed to determine G418 concentration for selection Started by Daniel5306, 13 Dec 2004	5 replies 6,120 views	Helios 10 Nov 2009
classic cloning with restriction enzymes into empty pcDNA3.1D Started by Euterpa12, 17 May 2013 cloning, pcDNA3.1	1 reply 44 views	bob1 17 May 2013
Cloning large fragments Started by bknm, 15 May 2013 Cloning, PCR, large inserts	1 reply 66 views	Curtis 16 May 2013
Transformation colonies does not contain insert Started by Sandy Fowler, 08 May 2013 transformation, Ligation, Cloning	3 replies 112 views	bob1 09 May 2013
Blunt cloning - No insert, despite lots of colonies Started by rxzlmn, 06 May 2013 failed cloning	3 replies 114 views	Curtis 09 May 2013
Need urgent advise.. Started by M.T2013, 29 Apr 2013 cloning	3 replies 163 views	JYaron 13 May 2013
problem with sequencing and mapping Started by ahmadfathi, 27 Apr 2013 sequencing	0 replies 104 views	ahmadfathi 27 Apr 2013
Cloning Mitochondrial Targeting Sequence Started by speci, 27 Apr 2013	4 replies 156 views	speci 29 Apr 2013
problem in cloning Started by gdbiomed, 25 Apr 2013	2 replies 135 views	phage434 25 Apr 2013
TA cloning: ligation problem or toxic construct? Started by DrLeo, 23 Apr 2013 TA cloning	4 replies 145 views	DrLeo 24 Apr 2013
Reappearing band shifts/smears after double digest and gel purification Started by muLab, 23 Apr 2013 restriction, smear, NotI, SbfI and 1 more...	2 replies 154 views	muLab 23 Apr 2013
Do I need to sequence after digestion? Started by smndtupidisaftr, 22 Apr 2013	9 replies 178 views	pito 24 Apr 2013
quick change mutagenesis...... Started by kartiga natarajan, 20 Apr 2013 Help me out plz......... 1 2	Hot 15 replies 247 views	GNANA 29 Apr 2013
difficulty to clone and/or express proteases. Toxicity? Started by monicalvi, 19 Apr 2013 protease, dh5 alpha, induction and 1 more...	0 replies 192 views	monicalvi 19 Apr 2013
Problem with double digestion: one enzyme is not working Started by Sandy143, 15 Apr 2013 BamHI, HindII, Double digestion	8 replies 273 views	Sandy143 21 Apr 2013