Molecular Cloning

Pinned  Sequential restriction digestion of plasmid

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Pinned  Problems with Stratagene Site Directed Mutagenesis

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Pinned  Creating empty vector by self-ligation

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Pinned  Tips on ethanol wash after nucleic acid precipitation

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Pinned  ds-oligo cloning/ligation issues

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Pinned  What is the biggest insert for a viral vector?

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Pinned  Smear in my restriction digest

Pinned  Help with 3-way ligation

Pinned  How to transfect two plasmids in a cell

Pinned  Time needed to determine G418 concentration for selection

Pinned  Old threads on Molecular Cloning are achived here

Did my EcoRI restriction digestion work? picture included!

Started by Fluffy, 27 Jun 2012   ecori, restriction, digestion

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My battle with BamHI

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Gel purification of vector DNA - low yield

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No product after restriction digestion

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vivo expression technology

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What does this look like to you?

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Odd point mutations

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primer Tm is too high, how tu get pcr product

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Help with cloning!.

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Plasmid-Stability? Fragment-Deletion? I'm in dire need to identify and solve

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pBR322 restriction digest

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Ligation

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Restriction enzyme digest

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Troublshooting failed transformation

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