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Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

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Topic	Stats	Last Post Info
Issue with Xba1 and Dam methylation Started by Ahrenhase, 26 Mar 2013	7 replies 157 views	phage434 28 Mar 2013
Problem with 3 way ligation Started by Ahrenhase, 27 Mar 2013	2 replies 164 views	Ahrenhase 28 Mar 2013
Co-transformation Started by ahmadfathi, 09 Mar 2013	2 replies 177 views	ahmadfathi 26 Mar 2013
Enzyme cutting: low efficiency Started by DrLeo, 26 Mar 2013	5 replies 134 views	DrLeo 26 Mar 2013
problem in cloning PCR primer design with restriction site Started by chn09, 12 Mar 2013	4 replies 178 views	phage434 26 Mar 2013
Restriction site rearrangement Started by relevant_username, 26 Mar 2013 cloning, restriction site and 2 more...	1 reply 128 views	phage434 26 Mar 2013
Must the terminator be in frame? Started by Spright, 23 Mar 2013	1 reply 126 views	Trof 23 Mar 2013
sequencing problems Started by lenkabo, 22 Mar 2013	2 replies 157 views	lenkabo 22 Mar 2013
pWPI vector - problems in transformation Started by smemorina, 21 Mar 2013	0 replies 108 views	smemorina 21 Mar 2013
Question regarding yeast strain with ura3-52 mutation Started by Daniel0306, 07 Mar 2013 Yeast, selectable marker and 2 more...	1 reply 165 views	HOYAJM 20 Mar 2013
Maintainence of transformed cells Started by criscastells, 14 Mar 2013	5 replies 174 views	bob1 14 Mar 2013
Restriction digest band shift? Started by miketibs, 13 Mar 2013	1 reply 131 views	jerryshelly1 13 Mar 2013
quick change mutagenesis...... Started by kartiga natarajan, 12 Mar 2013	1 reply 109 views	GNANA 12 Mar 2013
Help for pGEM-T easy vector ligation problem Started by biovinz, 10 Mar 2013	2 replies 159 views	biovinz 12 Mar 2013
cloneJet (Fermentas) ligation buffer "stability" Started by talianka, 11 Mar 2013	1 reply 130 views	bob1 11 Mar 2013
Problems regarding point mutations.... Started by kartiga natarajan, 08 Mar 2013 Trouble in PCR	4 replies 158 views	kartiga natarajan 10 Mar 2013
Vector insert ratio in cloning Started by harry348, 07 Mar 2013 vector, insert, ratio	3 replies 210 views	harry348 07 Mar 2013
CIAP dilution Started by harry348, 02 Nov 2012	5 replies 271 views	jerryshelly1 07 Mar 2013
Oligo insert cloning Started by 2xzwei, 06 Mar 2013	3 replies 147 views	Trof 07 Mar 2013
polymerase to use for cloning Started by srpres, 06 Mar 2013 cloning polymerase	4 replies 166 views	bob1 06 Mar 2013
How to select competent E. coli cells? Started by xxcici, 01 Mar 2013 plasmid transformation and 1 more...	7 replies 227 views	bob1 06 Mar 2013
Problem with ligation using pJET and pGEM for clone library Started by Procyon, 31 Jan 2013	9 replies 264 views	Procyon 06 Mar 2013
Poor expression in my cloned vector (pMSCV-PIG) Started by cancerous, 05 Mar 2013 pMSCV-PIG	1 reply 119 views	bob1 05 Mar 2013
Lambda PR promoter sequence Started by biovinz, 05 Mar 2013	0 replies 100 views	biovinz 05 Mar 2013
ligating blunt ends of a linearized plasmid, is kinase necessary? Started by kaveh, 04 Mar 2013	1 reply 127 views	jerryshelly1 04 Mar 2013