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Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

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Topic	Stats	Last Post Info
Reusing LB/amp/xgal plates (also, how long can e. coli be in the incubator for?) Started by Soda, 11 Jul 2009	4 replies 4,964 views	leelee 13 Jul 2009
Need help with dhfr gene Started by Nag, 13 Jul 2009	0 replies 1,039 views	Nag 13 Jul 2009
choosing restriction sites Started by beulah, 09 Jul 2009	5 replies 2,480 views	fishdoc 11 Jul 2009
Tag insertion Started by Sally-, 08 Jul 2009	5 replies 2,722 views	cellcounter 10 Jul 2009
Transduction Started by jen_23, 08 Jul 2009	3 replies 1,636 views	phage434 09 Jul 2009
Choice of vector for recombinant antibody expression Started by JulieL, 08 Jul 2009	0 replies 999 views	JulieL 08 Jul 2009
plasmid rescue (plasmids with incompatible ORI) Started by geetika, 07 Jul 2009	2 replies 1,500 views	molgen 08 Jul 2009
help wih the ligation Started by roshanbernard, 15 Jun 2009	7 replies 2,517 views	angad 08 Jul 2009
Storage of invitrogen top10 cells Started by epigenetics, 23 Jun 2009	11 replies 4,016 views	ram 08 Jul 2009
Vector with 2 promoters to drive expression of 2 genes Started by moorele, 07 Jul 2009	3 replies 1,703 views	moorele 07 Jul 2009
wrong bands after T-vector cloning Started by Ah-Do, 26 Jun 2009	3 replies 2,096 views	Ah-Do 05 Jul 2009
PCR problem from transformed TOPO TA vector Started by cloneguru, 01 Jul 2009	3 replies 1,804 views	jiajia1987 05 Jul 2009
nondenaturing PAGE to separate dna Started by deespike, 29 Jun 2009	4 replies 2,220 views	Qundo12 02 Jul 2009
urgent help regarding wanner method trouble shooting Started by sunni, 26 Jun 2009	2 replies 1,368 views	leelee 30 Jun 2009
DE3 Smegmatis strains Started by gee4s, 30 Jun 2009	0 replies 846 views	gee4s 30 Jun 2009
two step digestion Started by roshanbernard, 24 Jun 2009	6 replies 2,371 views	analynsarte 28 Jun 2009
Xho1 doesn't digest pEGFP.N2 completely, why? Started by Curtis, 27 Jun 2009	6 replies 2,308 views	HomeBrew 28 Jun 2009
Ligation issue: Does vector size matter? Started by Qundo12, 19 Jun 2009	10 replies 3,723 views	Qundo12 25 Jun 2009
No product after restriction digestion Started by xyz, 08 Jun 2009 1 2	Hot 26 replies 7,572 views	hanming86 25 Jun 2009
Help with cloning Started by Penguin, 19 Jun 2009	6 replies 2,620 views	Rsm 23 Jun 2009
How to exchange antibiotic resistance markers on a plasmid for E. coli Started by amano, 16 Jun 2009	2 replies 1,868 views	warsel 22 Jun 2009
PCR screening of transformed bacterial colony Started by xyz, 15 Jun 2009	6 replies 2,573 views	Qundo12 22 Jun 2009
Self-Ligation of digested vector Started by Signal, 20 Jun 2009	2 replies 1,915 views	pcrman 20 Jun 2009
Some colonies, but not enough... Started by 303microbialist, 18 Jun 2009	7 replies 2,541 views	eldon 20 Jun 2009
Chloramphenicol resistance gene and Chemicomps E. coli (TOP10, Mach1) Started by ruiN, 18 Jun 2009	2 replies 1,649 views	phage434 19 Jun 2009