Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

Moderators wanted

BioForum needs more volunteer moderators to provide expert help to other members by participating in forum discussions and answering questions. If you are interested, please follow this link to apply.

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

Page 6 of 62
4
5
6
7
8

Please log in to post a topic
Mark this forum as read

Topic	Stats	Last Post Info
pET 32 (+) Started by Pangea, 13 Jan 2013 pET plus	1 reply 121 views	phage434 13 Jan 2013
Do you have to purify target vector after double digest from MCS piece Started by assembler01, 11 Jan 2013 MCS, double digest, purify and 1 more...	1 reply 175 views	HOYAJM 11 Jan 2013
Problem in cloning multiple shRNAs in pcDNA 3.1 Started by Indhu, 10 Jan 2013	0 replies 119 views	Indhu 10 Jan 2013
Problems with growing out transformants in liquid LB Started by Toastayy, 09 Jan 2013	3 replies 148 views	Toastayy 09 Jan 2013
Problem with cloning - what is wrong? Started by Guf, 04 Jan 2013 Cloning, dephopshorylation and 2 more...	6 replies 320 views	phage434 09 Jan 2013
Ethidiumbromide Started by Pangea, 05 Jan 2013 DNA EthBr	3 replies 201 views	pito 08 Jan 2013
GST TAG Started by Pangea, 03 Jan 2013 Boundary	3 replies 176 views	bob1 05 Jan 2013
TRYPSIN Started by Pangea, 04 Jan 2013 Trypsin cell culture pro	4 replies 166 views	Pangea 05 Jan 2013
Where to place the polyA signal? Started by wesida, 28 Dec 2012	1 reply 250 views	Curtis 28 Dec 2012
molecular cloning Started by Rampa, 11 Dec 2012 puc19	7 replies 329 views	phage434 23 Dec 2012
Genomic DNA extraction with SDS/LiOAc Started by Zanetti, 18 Dec 2012 gDNA, pcr, SDS/LiOAc	0 replies 335 views	Zanetti 18 Dec 2012
identification of target sequence Started by helpwithdna, 16 Dec 2012	3 replies 303 views	bob1 17 Dec 2012
Large quantity heat shock trouble? Started by jungskwon98, 14 Dec 2012 transformation, E. coli and 1 more...	3 replies 342 views	jungskwon98 17 Dec 2012
cloning pcr insert into plasmid Started by helena kyle, 16 Dec 2012 sticky end, pcr cloning	2 replies 331 views	helena kyle 17 Dec 2012
TOPO TA CLONING - MOLAR RATIO Started by Epigeneticist, 15 Dec 2012 TOPO TA CLONING and 2 more...	2 replies 310 views	Epigeneticist 15 Dec 2012
Cannot see insert with colony pcr Started by Smog187, 02 Dec 2012 Colony PCR, Cloning	6 replies 387 views	bob1 04 Dec 2012
help with a vector Started by shawn6022, 01 Dec 2012 pJDC15, mycobacterium	3 replies 244 views	phage434 02 Dec 2012
Ethidium precipitation in CsCl Maxiprep Started by BChic, 02 Dec 2012 CsCl, maxiprep, trouble, plasmid	1 reply 223 views	bob1 02 Dec 2012
Amplicon as Template in PCR for TOPO TA Cloning Started by Epigeneticist, 01 Dec 2012 PCR, TOPO TA CLONING, CLONING	10 replies 276 views	Trof 01 Dec 2012
Another cloning hell....no transformants Started by Schevchen, 27 Nov 2012	1 reply 286 views	phage434 27 Nov 2012
SV40 promoter Started by mbgene, 26 Nov 2012 sv40 promoter	0 replies 193 views	mbgene 26 Nov 2012
Agrobacterium LBA4404 transformation Started by sukphin, 22 Nov 2012	1 reply 252 views	phage434 23 Nov 2012
TOPO TA Cloning Troubleshooting Started by Epigeneticist, 19 Nov 2012 TOPO TA CLONING, CLONING and 3 more...	1 reply 349 views	Trof 20 Nov 2012
How do you screen promoter regions if you can only PCR a few thousand bp Started by Ahrenhase, 19 Nov 2012	1 reply 196 views	pcrman 19 Nov 2012
Problems with Ecoli glycerol stock Started by krisw, 05 Nov 2012	6 replies 259 views	pito 19 Nov 2012