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Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

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Topic	Stats	Last Post Info
What is a fos-pomoter and what does SV40 late pA do? Started by Joanna_Andersen, 10 Feb 2013 cloning, promoter, vector	0 replies 181 views	Joanna_Andersen 10 Feb 2013
Stuck from last august! need help urgently - cloning and pcr ! Started by mansi368, 05 Feb 2013	7 replies 232 views	bob1 06 Feb 2013
QIAGEN Spin Columns and Minipreps - Alternatives?! Started by Luria Bertani, 14 Aug 2012	13 replies 646 views	Lisa Hsu 06 Feb 2013
How to set up simultaneous digestion? Started by Guf, 01 Feb 2013 plasmid, vector, digestion	3 replies 251 views	phage434 04 Feb 2013
I have a 96 tandem array repeat clone but need half of them in new clone Started by nvramakanth, 22 Jan 2013	4 replies 172 views	phage434 04 Feb 2013
Storing NovaBlue Transformation Reactions in SOC Started by Ihla, 01 Feb 2013 Transformation	1 reply 186 views	bob1 01 Feb 2013
adding Nde1 and Xho 1 restriction site in PCR primer Started by chn09, 31 Jan 2013 primer design	2 replies 175 views	bob1 31 Jan 2013
Freeze transformation mixture? Started by tihong10, 28 Jan 2013 transformation, freezing	2 replies 231 views	tihong10 31 Jan 2013
The Issue of Freezing and Using colonies of Tranformed DH5a Started by tihong10, 27 Jan 2013 DH5a, transformation, freezing	7 replies 255 views	tihong10 31 Jan 2013
Why tranform using DH5 alpha -> SM10 λpir?? Started by tihong10, 31 Oct 2012 DH5, alpha, SM10 λpir	3 replies 270 views	tihong10 31 Jan 2013
Really strange problem with sequencing and plasmid purification! Started by pokemon, 11 Jan 2011	8 replies 1,240 views	sd_labber 30 Jan 2013
Cutting my plasmid made it larger Started by PJB, 28 Jan 2013	7 replies 252 views	PJB 29 Jan 2013
Amplification of CAG promoter problems Started by leyshmanioz, 26 Jan 2013 no product	3 replies 200 views	phage434 27 Jan 2013
PIR 1 Competent Cells Started by indigo2, 09 Feb 2010	1 reply 1,041 views	Candace Johnson 24 Jan 2013
restriction enzyme Started by harry348, 24 Jan 2013	2 replies 142 views	harry348 24 Jan 2013
Colony-PCR workaround Started by Spright, 22 Jan 2013	3 replies 169 views	Trof 24 Jan 2013
Can I pause a bacterial culture at 4°C Started by sabi67, 22 Jan 2013	2 replies 183 views	sabi67 23 Jan 2013
Recreate original plasmid by cutting out insert Started by schaapje_86, 23 Jan 2013	2 replies 136 views	phage434 23 Jan 2013
problem with plasmid digestion Started by Ensyeh, 16 Jan 2013 plasmid	5 replies 231 views	neuron 21 Jan 2013
TOPO TA CLONING - LIGATION STEP Started by Epigeneticist, 20 Jan 2013 TOPO TA CLONING, LIGATION	6 replies 219 views	Epigeneticist 20 Jan 2013
posting sequences of plasmid? Started by lyok, 19 Jan 2013	3 replies 186 views	phage434 19 Jan 2013
Restriction Enzyme Digest not working Started by Tom Jerry, 18 Jan 2013 Restriction Digestion	2 replies 208 views	phage434 19 Jan 2013
cloning: band at different site than desired after RE digestion Started by shivu11, 04 Jan 2013 Clone, re digestion, ligation	11 replies 292 views	shivu11 18 Jan 2013
Incomplete digestion of plasmid with single enzyme Started by neuron, 17 Jan 2013 how many bands?	1 reply 135 views	bob1 17 Jan 2013
After ligation,I found that my colony on amplicilin plate have not insert gene Started by sagebook, 17 Jan 2013 ligation digestion	1 reply 152 views	bob1 17 Jan 2013