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Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

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Topic	Stats	Last Post Info
Answered Ligate a very small insert into a vector Started by Thomson, 13 Mar 2018 insert, small dna fragment and 2 more...	3 replies 240 views	bob1 14 Mar 2018
Answered The right lentivirus plasmid for overexpression in human cells Started by TheFOrsh, 28 Feb 2015	7 replies 1,176 views	5280 18 Mar 2016
Answered Need to add 12 bp at the beginning of the sequence Started by lexi1096, 22 Feb 2016	4 replies 746 views	lexi1096 08 Mar 2016
Answered Mutation in one chain of homodimer Started by madmaddy77, 07 Apr 2015 mutation, cloning	4 replies 1,056 views	labtastic 29 May 2015
Answered RE digestion and ligation troubleshooting. Started by Wek, 22 Apr 2015	1 reply 610 views	phage434 23 Apr 2015
Answered TA Cloning Started by Meg P. Anula, 14 Mar 2015 TA vector, vector, invitrogen and 1 more...	11 replies 1,310 views	Trof 23 Mar 2015
Answered Suitable ratio of vector and insert in cloning Started by Meg P. Anula, 01 Mar 2015 cloning, vector, insert, ligation	5 replies 1,077 views	bob1 04 Mar 2015
Answered Bacteria grow on antibiotic selective plate but plasmid extracted showed no dna Started by Meg P. Anula, 09 Feb 2015 plasmid, dna insert, cloning and 2 more...	6 replies 1,252 views	Meg P. Anula 11 Feb 2015
Answered question about Clonetech Retro-X Tet-on system Started by jackster101, 12 Jan 2015 inducible system	3 replies 888 views	jackster101 14 Jan 2015
Answered No colonies after self Circularization ligation Started by KhaleesiDany, 22 Oct 2014 ligation, cloning, vector and 2 more...	2 replies 1,171 views	AskDNA 07 Nov 2014
Answered Ligation: is it possible to ligate two vector backbones together Started by KhaleesiDany, 25 Aug 2014 cloning, vector, insert, ligation and 1 more...	11 replies 1,318 views	phage434 27 Aug 2014
Answered How efficient is the 2A sequence Started by Ahrenhase, 10 Feb 2014	2 replies 579 views	Ahrenhase 16 Feb 2014
Answered Incubation period after heat shock at transformation--reason? Started by luckyjade, 24 Nov 2013	3 replies 729 views	luckyjade 28 Nov 2013
Answered New ordered Restriction Enzymes not working, need help! Started by Celeste, 23 Oct 2013	2 replies 962 views	Celeste 23 Oct 2013
Answered Help! Plasmid resistant to restriction digest after acquiring Flag tag Started by KristinaZ, 13 Sep 2013 Restriction digest, cloning and 2 more...	3 replies 897 views	KristinaZ 25 Sep 2013
Answered Type of polymerases compatible for TA cloning Started by pmggms2, 11 Sep 2013 cloning, TOPO plasmid	7 replies 870 views	phage434 17 Sep 2013