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Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

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Topic	Stats	Last Post Info
Pinned Sequential restriction digestion of plasmid Started by shimshady, 21 Feb 2009 1 2 3 4	Hot 58 replies 35,972 views	mahrak 27 Aug 2013
Pinned Problems with Stratagene Site Directed Mutagenesis Started by yean_ny_nie, 07 Apr 2009 1 2 3	Hot 36 replies 26,658 views	fighter 25 May 2012
Pinned ds-oligo cloning/ligation issues Started by GilsonGirl, 06 Mar 2009 1 2	Hot 18 replies 9,375 views	Trof 03 Apr 2012
Pinned What is the biggest insert for a viral vector? Started by Kami23, 06 Feb 2009 1 2	Hot 15 replies 13,192 views	wincel 24 Sep 2010
Pinned Smear in my restriction digest Started by panda, 07 May 2009	12 replies 10,553 views	wincel 24 Sep 2010
Pinned Tips on ethanol wash after nucleic acid precipitation Started by mario2004, 13 Mar 2005 1 2	Hot 22 replies 21,254 views	Maddie 30 Jul 2010
Pinned Creating empty vector by self-ligation Started by chijing, 06 Apr 2009 1 2 3	Hot 31 replies 30,032 views	kellyhayes 23 Nov 2009
Pinned Time needed to determine G418 concentration for selection Started by Daniel5306, 13 Dec 2004	5 replies 6,715 views	Helios 10 Nov 2009
Pinned How to transfect two plasmids in a cell Started by muchmoa, 04 May 2009	10 replies 9,925 views	laurequillo 25 Sep 2009
Pinned Help with 3-way ligation Started by PhilS, 24 Feb 2009	11 replies 10,632 views	PhilS 15 Mar 2009
Pinned Old threads on Molecular Cloning are achived here Started by bioforum, 03 Feb 2009	0 replies 5,613 views	bioforum 03 Feb 2009
E.coli culture in -4c for 2 hours means it will affect or not? what happened in Started by thil, Today, 12:34 AM cloning, molecular cloning	1 reply 34 views	bob1 Today, 11:44 AM
Problem in plasmid digestion after transformation Started by aasim, 10 Mar 2014	4 replies 63 views	aasim Yesterday, 02:46 AM
Lac Promoter activity in P. aeruginosa Started by tihong10, 10 Mar 2014	0 replies 27 views	tihong10 10 Mar 2014
Resctriction analysis Started by Student30, 09 Mar 2014	1 reply 40 views	bob1 09 Mar 2014
Please comment on my Cloning Plan Started by tihong10, 07 Mar 2014	1 reply 82 views	jerryshelly1 07 Mar 2014
Gibson/SLIC question Started by Rsm, 05 Mar 2014	2 replies 65 views	Rsm 07 Mar 2014
Accidentally forgot a base making primers for InFusion (Gibson); Will it work Started by Ahrenhase, 06 Mar 2014	0 replies 49 views	Ahrenhase 06 Mar 2014
How to determine where, in the genome, my transgene inserted Started by Ahrenhase, 28 Feb 2014	4 replies 161 views	phage434 02 Mar 2014
How can addgene sell plasmids with patented technology Started by Ahrenhase, 26 Feb 2014	2 replies 120 views	Ahrenhase 28 Feb 2014
no colonies, ever! Started by oopsyda1sy, 27 Feb 2014 transformation, ligation, cloning and 1 more...	3 replies 148 views	bob1 27 Feb 2014
fluorescent tag on membrane protein Started by mengkong2, 26 Feb 2014 fluorescent, cloning, plasmid	1 reply 92 views	bob1 27 Feb 2014
no insert issue Started by plasmamembrane, 21 Feb 2014	9 replies 161 views	plasmamembrane 24 Feb 2014
Considerations on the design of a transgene Started by Ahrenhase, 20 Feb 2014	1 reply 106 views	perneseblue 22 Feb 2014
SacB inefficient in Pseudomonas, stuck at merodiploid Started by thefallengrace, 22 Feb 2014 SacB, Merodiploi, Pseudomonas and 2 more...	0 replies 99 views	thefallengrace 22 Feb 2014