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Molecular Cloning
Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...
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Sequential restriction digestion of plasmidStarted by shimshady, 21 Feb 2009 |
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Problems with Stratagene Site Directed MutagenesisStarted by yean_ny_nie, 07 Apr 2009 |
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ds-oligo cloning/ligation issuesStarted by GilsonGirl, 06 Mar 2009 |
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What is the biggest insert for a viral vector?Started by Kami23, 06 Feb 2009 |
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Smear in my restriction digestStarted by panda, 07 May 2009 |
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Tips on ethanol wash after nucleic acid precipitationStarted by mario2004, 13 Mar 2005 |
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Creating empty vector by self-ligationStarted by chijing, 06 Apr 2009 |
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Time needed to determine G418 concentration for selectionStarted by Daniel5306, 13 Dec 2004 |
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How to transfect two plasmids in a cellStarted by muchmoa, 04 May 2009 |
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Help with 3-way ligationStarted by PhilS, 24 Feb 2009 |
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Old threads on Molecular Cloning are achived hereStarted by bioforum, 03 Feb 2009 |
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Plasmid distributors for Luciferase Assay?Started by Helder_90, 03 Apr 2018 |
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Ligate a very small insert into a vectorStarted by Thomson, 13 Mar 2018  insert, small dna fragment and 2 more...
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Restriction enzymeStarted by Huongnguyen.miss, 20 Dec 2017 |
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Plasmid dna sizeStarted by Angeline, 01 Dec 2017 |
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Determining optimal digestion time for partial digestionStarted by dentategyrus, 18 Oct 2011 |
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p1 transduction into recA- strainStarted by Raygoza, 21 Sep 2017 |
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An unusual "buffer" in a protocol for making chemically competent E.coliStarted by Cistron, 06 Sep 2017 E.coli, competent cells
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Can someone please explain this to me?Started by Questions, 20 May 2016 |
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Express two different membrane proteins on the same promoterStarted by anonyvous, 25 Jul 2017 |
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Help with making a DimerStarted by kenny17, 09 Jul 2017 cloning, ssDNA, pGEM3zf+, MSV
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[Overexpression] Without upstream transcription terminatorStarted by lactamase, 09 May 2017 |
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![[Overexpression] Without upstream transcription terminator - last post by lactamase](http://www.gravatar.com/avatar/ed95266ad5fa49215efc400961aeb7f9?s=100&d=http%3A%2F%2Fwww.protocol-online.org%2Fforums%2F%2Fpublic%2Fstyle_images%2Fmaster%2Fprofile%2Fdefault_large.png)
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Using modified CFP and YFP plasmids for FRET assayStarted by maarts, 11 May 2017 pAquaN1, Citrine-C1, gfp, cfp and 1 more...
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Molecular cloning into pGhost5Started by Lana, 07 Apr 2017 cloning
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How large need homology arms to be?Started by Luca, 07 Feb 2017 CRISPR/Cas9 and 2 more...
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