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Molecular Cloning
Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...
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Sequential restriction digestion of plasmidStarted by shimshady, 21 Feb 2009 |
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Problems with Stratagene Site Directed MutagenesisStarted by yean_ny_nie, 07 Apr 2009 |
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ds-oligo cloning/ligation issuesStarted by GilsonGirl, 06 Mar 2009 |
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What is the biggest insert for a viral vector?Started by Kami23, 06 Feb 2009 |
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Smear in my restriction digestStarted by panda, 07 May 2009 |
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Tips on ethanol wash after nucleic acid precipitationStarted by mario2004, 13 Mar 2005 |
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Creating empty vector by self-ligationStarted by chijing, 06 Apr 2009 |
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Time needed to determine G418 concentration for selectionStarted by Daniel5306, 13 Dec 2004 |
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How to transfect two plasmids in a cellStarted by muchmoa, 04 May 2009 |
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Help with 3-way ligationStarted by PhilS, 24 Feb 2009 |
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Old threads on Molecular Cloning are achived hereStarted by bioforum, 03 Feb 2009 |
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Accidentally forgot a base making primers for InFusion (Gibson); Will it workStarted by Ahrenhase, Today, 12:34 PM |
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Gibson/SLIC questionStarted by Rsm, Yesterday, 05:52 AM |
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How to determine where, in the genome, my transgene insertedStarted by Ahrenhase, 28 Feb 2014 |
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How can addgene sell plasmids with patented technologyStarted by Ahrenhase, 26 Feb 2014 |
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no colonies, ever!Started by oopsyda1sy, 27 Feb 2014  transformation, ligation, cloning and 1 more...
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fluorescent tag on membrane proteinStarted by mengkong2, 26 Feb 2014 fluorescent, cloning, plasmid
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no insert issueStarted by plasmamembrane, 21 Feb 2014 |
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Considerations on the design of a transgeneStarted by Ahrenhase, 20 Feb 2014 |
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SacB inefficient in Pseudomonas, stuck at merodiploidStarted by thefallengrace, 22 Feb 2014 SacB, Merodiploi, Pseudomonas and 2 more...
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How to obtain Vectors used in articlesStarted by tihong10, 18 Feb 2014 procuring, vectors
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Gateway vectorsStarted by jackster101, 01 Feb 2014 pENTR 221
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Pcr primersStarted by jackster101, 06 Feb 2014 Cloning question
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How efficient is the 2A sequenceStarted by Ahrenhase, 10 Feb 2014 |
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Slightly more efficient promoter?Started by PJB, 14 Feb 2014 |
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