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Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

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Topic	Stats	Last Post Info
Pinned Sequential restriction digestion of plasmid Started by shimshady, 21 Feb 2009 1 2 3 4	Hot 58 replies 51,442 views	mahrak 27 Aug 2013
Pinned Problems with Stratagene Site Directed Mutagenesis Started by yean_ny_nie, 07 Apr 2009 1 2 3	Hot 36 replies 39,239 views	fighter 25 May 2012
Pinned ds-oligo cloning/ligation issues Started by GilsonGirl, 06 Mar 2009 1 2	Hot 18 replies 19,501 views	Trof 03 Apr 2012
Pinned What is the biggest insert for a viral vector? Started by Kami23, 06 Feb 2009 1 2	Hot 15 replies 23,438 views	wincel 24 Sep 2010
Pinned Smear in my restriction digest Started by panda, 07 May 2009	12 replies 19,009 views	wincel 24 Sep 2010
Pinned Tips on ethanol wash after nucleic acid precipitation Started by mario2004, 13 Mar 2005 1 2	Hot 22 replies 32,262 views	Maddie 30 Jul 2010
Pinned Creating empty vector by self-ligation Started by chijing, 06 Apr 2009 1 2 3	Hot 31 replies 42,282 views	kellyhayes 23 Nov 2009
Pinned Time needed to determine G418 concentration for selection Started by Daniel5306, 13 Dec 2004	5 replies 14,557 views	Helios 10 Nov 2009
Pinned How to transfect two plasmids in a cell Started by muchmoa, 04 May 2009	9 replies 17,983 views	laurequillo 25 Sep 2009
Pinned Help with 3-way ligation Started by PhilS, 24 Feb 2009	11 replies 18,536 views	PhilS 15 Mar 2009
Pinned Old threads on Molecular Cloning are achived here Started by bioforum, 03 Feb 2009	0 replies 13,259 views	bioforum 03 Feb 2009
High copy vs low copy plasmids: which one causes less toxicity? Started by Curtis, 12 Jan 2019	2 replies 70 views	Trof Today, 01:22 AM
Plasmid analysis in limited conditions Started by Trof, 02 May 2018	6 replies 692 views	OldCloner 02 Jan 2019
low copy number plasmid purification kit Started by nightingale, 12 Sep 2018 any suggestions ??	4 replies 493 views	Curtis 16 Oct 2018
Blunt-end ligation: what if the vector and insert are both phosphorylated? Started by Curtis, 16 Sep 2018	4 replies 266 views	OldCloner 18 Sep 2018
Transformation Started by D.Luffy, 24 Apr 2018	3 replies 568 views	OldCloner 12 Sep 2018
Weird results with bacterial transformation Started by rmbio, 16 May 2015 Contamination	11 replies 2,162 views	OldCloner 12 Sep 2018
Determining optimal digestion time for partial digestion Started by dentategyrus, 18 Oct 2011	2 replies 1,261 views	OldCloner 20 Jul 2018
Why do I get two bands on the gel of a digested PCR product after purification w Started by Delimar Rodriguez, 27 Apr 2018 molecular, cloning, digest, pcr and 1 more...	1 reply 877 views	OldCloner 20 Jul 2018
Appearance of a higher molecular weight band after plasmid isolation? (NOT chros Started by DaRQsiDe, 02 Jan 2014 Plasmid isolation and 2 more...	2 replies 1,207 views	Trof 09 Jul 2018
Plasmid dna size Started by Angeline, 01 Dec 2017	3 replies 870 views	OldCloner 13 Jun 2018
Answered Neomycin/Kanamycin Resistant gene Started by Thomson, 10 May 2018 neomycin, kanamycin and 1 more...	2 replies 486 views	Thomson 13 May 2018
Troubleshooting with restriction enzymes during cloning Started by liligompac, 25 Apr 2018 cloning, pgem t easy and 2 more...	4 replies 1,475 views	liligompac 26 Apr 2018
Plasmid distributors for Luciferase Assay? Started by Helder_90, 03 Apr 2018	2 replies 482 views	Helder_90 03 Apr 2018
Answered Ligate a very small insert into a vector Started by Thomson, 13 Mar 2018 insert, small dna fragment and 2 more...	3 replies 694 views	bob1 14 Mar 2018