Molecular Cloning

Pinned  Problems with Stratagene Site Directed Mutagenesis

• 1
• 2
• 3

Pinned  ds-oligo cloning/ligation issues

• 1
• 2

Pinned  Sequential restriction digestion of plasmid

• 1
• 2
• 3
• 4

Pinned  What is the biggest insert for a viral vector?

• 1
• 2

Pinned  Smear in my restriction digest

Pinned  Creating empty vector by self-ligation

• 1
• 2
• 3

Pinned  Time needed to determine G418 concentration for selection

Pinned  How to transfect two plasmids in a cell

Pinned  Help with 3-way ligation

Pinned  Old threads on Molecular Cloning are achived here

Creating a pGEM-T easy + insert sequence map

Started by levuccio, 21 May 2012   pGEM-T easy, seqbuilder, cloning

screening for GPF

Started by Tracy0110, Yesterday, 05:20 PM   GFP, pGreen, vector

Colony PCR works but DNA yeild very low after miniprep

Started by elk2019, 19 May 2012   colony PCR, low DNA yeild

where to get vector overexpressing caveolin-1?

gateway cloning: frameshift

pINDUCER series

Started by yosta, 18 May 2012   lentiviral vector, cloning

12.4kb vector 6.7kb insert

Started by val80, 12 May 2012   ligation, large vector

pGEM-T Easy vector contamination

Started by manicd, 07 May 2012   pGEM-T Easy, contamination

Cloning into lentiviral vector

LZRS vector details

Started by ssatl, 15 May 2012   LZRS, promoter, expression

Stable transfection

an effective way to do a yeast colony pcr

Using normal competent cells in electroporation method?

blunt end restriction out of recognition site

Started by snusnu, 08 May 2012   restriction, blunt end, cloning

I need bynary vector pART27

Started by manueliux_06, 02 May 2012   vector, pART27