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Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

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Topic	Stats	Last Post Info
Pinned Sequential restriction digestion of plasmid Started by shimshady, 21 Feb 2009 1 2 3 4	Hot 58 replies 55,211 views	mahrak 27 Aug 2013
Pinned Problems with Stratagene Site Directed Mutagenesis Started by yean_ny_nie, 07 Apr 2009 1 2 3	Hot 36 replies 42,218 views	fighter 25 May 2012
Pinned ds-oligo cloning/ligation issues Started by GilsonGirl, 06 Mar 2009 1 2	Hot 18 replies 22,165 views	Trof 03 Apr 2012
Pinned What is the biggest insert for a viral vector? Started by Kami23, 06 Feb 2009 1 2	Hot 15 replies 26,042 views	wincel 24 Sep 2010
Pinned Smear in my restriction digest Started by panda, 07 May 2009	12 replies 21,324 views	wincel 24 Sep 2010
Pinned Tips on ethanol wash after nucleic acid precipitation Started by mario2004, 13 Mar 2005 1 2	Hot 22 replies 34,914 views	Maddie 30 Jul 2010
Pinned Creating empty vector by self-ligation Started by chijing, 06 Apr 2009 1 2 3	Hot 31 replies 45,290 views	kellyhayes 23 Nov 2009
Pinned Time needed to determine G418 concentration for selection Started by Daniel5306, 13 Dec 2004	5 replies 16,805 views	Helios 10 Nov 2009
Pinned How to transfect two plasmids in a cell Started by muchmoa, 04 May 2009	9 replies 20,259 views	laurequillo 25 Sep 2009
Pinned Help with 3-way ligation Started by PhilS, 24 Feb 2009	11 replies 20,848 views	PhilS 15 Mar 2009
Pinned Old threads on Molecular Cloning are achived here Started by bioforum, 03 Feb 2009	0 replies 15,500 views	bioforum 03 Feb 2009
Generating a cell line using sleeping beauty transposase and a gene that contain Started by derkuli, 13 Sep 2020 Cloning, SfiI, Sleeping beauty	0 replies 201 views	derkuli 13 Sep 2020
HELP: Validation of a polyclonal knock out (KO) cell line when you dont have a g Started by Natalia KM, 13 Aug 2020 knock outKO, validation, Crispr and 1 more...	6 replies 517 views	Natalia KM 20 Aug 2020
Trouble transforming pET28a in E. coli BL21(DE3) Started by SarveshSurve, 04 Feb 2020 transformation, vector and 2 more...	5 replies 681 views	OldCloner 16 Feb 2020
Choosing a common exon in GOI to design your guides Started by Natalia KM, 11 Oct 2019 Crisprexon, guide RNA	1 reply 1,650 views	Jon Moulton 18 Dec 2019
Cloning protein of interest into lentiviral vector - help - I have no experience Started by Natalia KM, 12 Jul 2019 molecular cloning, plasmids 1 2	Hot 20 replies 1,333 views	Natalia KM 17 Sep 2019
Designing Crispr guides and cloning into appropriate vectors Started by Natalia KM, 13 Sep 2019 molecular cloning, Crispr/Cas9 and 2 more...	1 reply 2,113 views	OldCloner 13 Sep 2019
plasmid purification Started by nightingale, 22 Jul 2019 nanodrop vs. agarose gel	4 replies 1,061 views	nightingale 25 Jul 2019
Insert in frame Started by Sciencecraze, 01 Mar 2019	1 reply 952 views	bob1 02 Mar 2019
Troubleshooting with restriction enzymes during cloning Started by liligompac, 25 Apr 2018 cloning, pgem t easy and 2 more...	5 replies 4,239 views	OldCloner 19 Feb 2019
PCRed-vector approach to tag switching Started by Trof, 11 Feb 2019	1 reply 542 views	Trof 11 Feb 2019
Cloning of an unknown gene Started by bio_kid, 13 Apr 2015 cloning, genome library and 1 more...	4 replies 1,764 views	OldCloner 22 Jan 2019
High copy vs low copy plasmids: which one causes less toxicity? Started by Curtis, 12 Jan 2019	2 replies 600 views	Trof 20 Jan 2019
Plasmid analysis in limited conditions Started by Trof, 02 May 2018	6 replies 1,381 views	OldCloner 02 Jan 2019
low copy number plasmid purification kit Started by nightingale, 12 Sep 2018 any suggestions ??	4 replies 1,090 views	Curtis 16 Oct 2018