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Molecular Cloning
Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...
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Sequential restriction digestion of plasmidStarted by shimshady, 21 Feb 2009 |
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Problems with Stratagene Site Directed MutagenesisStarted by yean_ny_nie, 07 Apr 2009 |
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ds-oligo cloning/ligation issuesStarted by GilsonGirl, 06 Mar 2009 |
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What is the biggest insert for a viral vector?Started by Kami23, 06 Feb 2009 |
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Smear in my restriction digestStarted by panda, 07 May 2009 |
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Tips on ethanol wash after nucleic acid precipitationStarted by mario2004, 13 Mar 2005 |
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Creating empty vector by self-ligationStarted by chijing, 06 Apr 2009 |
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Time needed to determine G418 concentration for selectionStarted by Daniel5306, 13 Dec 2004 |
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How to transfect two plasmids in a cellStarted by muchmoa, 04 May 2009 |
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Help with 3-way ligationStarted by PhilS, 24 Feb 2009 |
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Old threads on Molecular Cloning are achived hereStarted by bioforum, 03 Feb 2009 |
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Plasmid purification for Gibson AssemblyStarted by Yu Zhang, 09 Aug 2016  plasmid, purification and 1 more...
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TA cloning..Urgent Help!!Started by Mehjabeen, 07 Jun 2016 |
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Splicing DNA fragmentsStarted by rmbio, 09 Mar 2016 a stretch missing!
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Unexpected band in BL21 (DE3) plasmid isolationStarted by james21, 11 Jul 2016 plasmid, electophoresis, BL21
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Migration of circular DNA vs circular/crosslinked peptide in gelsStarted by labtastic, 11 Aug 2015 |
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Weird results with bacterial transformationStarted by rmbio, 16 May 2015 Contamination
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Problem with subcloning genescript synthesized genesStarted by FoTem21, 23 Jul 2015 |
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Using SDS for DNA electrophoresisStarted by Raygoza, 07 Sep 2015 |
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Cleaning loading dye for PCR sequencingStarted by Raygoza, 18 Sep 2015 PCR
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Lower Plasmid YieldsStarted by Missle, 29 Sep 2015 plasmid purification and 1 more...
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Problems with ligationStarted by Lfs, 09 Dec 2015 |
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Luciferase assay cloningStarted by Azk Blub Clima, 27 Dec 2015 |
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How stable is double digested plasmidStarted by Meg P. Anula, 20 Dec 2015 double digested, plasmid and 2 more...
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Selecting bacteria with PuromycinStarted by kwesi, 23 Dec 2015 cloning, puromycin, bacteria
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