Submit your paper to J Biol Methods today!
Molecular Cloning
Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...
- Please log in to post a topic
-
Mark this forum as read
 |
Pinned
Sequential restriction digestion of plasmidStarted by shimshady, 21 Feb 2009 |
|
|
|
|
|
Pinned
Problems with Stratagene Site Directed MutagenesisStarted by yean_ny_nie, 07 Apr 2009 |
|
|
|
|
|
Pinned
ds-oligo cloning/ligation issuesStarted by GilsonGirl, 06 Mar 2009 |
|
|
|
|
|
Pinned
What is the biggest insert for a viral vector?Started by Kami23, 06 Feb 2009 |
|
|
|
|
|
Pinned
Smear in my restriction digestStarted by panda, 07 May 2009 |
|
|
|
|
|
Pinned
Tips on ethanol wash after nucleic acid precipitationStarted by mario2004, 13 Mar 2005 |
|
|
|
|
|
Pinned
Creating empty vector by self-ligationStarted by chijing, 06 Apr 2009 |
|
|
|
|
|
Pinned
Time needed to determine G418 concentration for selectionStarted by Daniel5306, 13 Dec 2004 |
|
|
|
|
|
Pinned
How to transfect two plasmids in a cellStarted by muchmoa, 04 May 2009 |
|
|
|
|
|
Pinned
Help with 3-way ligationStarted by PhilS, 24 Feb 2009 |
|
|
|
|
|
Pinned
Old threads on Molecular Cloning are achived hereStarted by bioforum, 03 Feb 2009 |
|
|
|
 |
primer design with restriction enzymeStarted by SF_HK, Today, 12:45 AM  bases flanking restricti
|
|
|
|
|
|
Restriction to remove backgroundStarted by Raygoza, 18 Jul 2014 |
|
|
|
|
|
vector propagation in E coliStarted by plasmamembrane, 19 Jul 2014 |
|
|
|
|
|
How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vectorStarted by nrl81182, 18 Jul 2014 |
|
|
|
|
|
tn5 transposonStarted by Raygoza, 18 Jul 2014 |
|
|
|
|
|
My battle with BamHIStarted by georgiadave, 23 Mar 2010 |
|
|
|
|
|
Restriction and ligation time intervalStarted by Raygoza, 11 Jul 2014 ligation, restriction
|
|
|
|
|
|
Lentivirus copy numberStarted by Zduan, 08 Jul 2014 lentivirus, experimental design and 3 more...
|
|
|
|
|
|
Problem shrna cloning, bad sequencing resultsStarted by Finouu0106, 09 Jul 2014 shrna, cloning, sequencing
|
|
|
|
|
|
5' RLM RACEStarted by Epigeneticist, 09 Jul 2014 TOTAL RNA, PCR, RNA PURIFICATION and 1 more...
|
|
|
|
|
|
TET off plasmid recommendationsStarted by ramblingrat, 07 Jul 2014 TET off, doxycyline
|
|
|
|
|
|
Restriction-free cloning to exchange gene in vector backboneStarted by EmilieKB, 02 Jul 2014 cloning gfp RE-free
|
|
|
|
|
|
pcDNA 3.1 Directional TOPO clone issuesStarted by Muzamil, 01 Jul 2014 |
|
|
|
|
|
Genomic ß-Lactamase? Blue White screening not workingStarted by 2xzwei, 03 Jul 2014 |
|
|
This forum led by
