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Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

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Topic	Stats	Last Post Info
Pinned Sequential restriction digestion of plasmid Started by shimshady, 21 Feb 2009 1 2 3 4	Hot 58 replies 39,731 views	mahrak 27 Aug 2013
Pinned Problems with Stratagene Site Directed Mutagenesis Started by yean_ny_nie, 07 Apr 2009 1 2 3	Hot 36 replies 29,045 views	fighter 25 May 2012
Pinned ds-oligo cloning/ligation issues Started by GilsonGirl, 06 Mar 2009 1 2	Hot 18 replies 10,945 views	Trof 03 Apr 2012
Pinned What is the biggest insert for a viral vector? Started by Kami23, 06 Feb 2009 1 2	Hot 15 replies 14,865 views	wincel 24 Sep 2010
Pinned Smear in my restriction digest Started by panda, 07 May 2009	12 replies 11,617 views	wincel 24 Sep 2010
Pinned Tips on ethanol wash after nucleic acid precipitation Started by mario2004, 13 Mar 2005 1 2	Hot 22 replies 23,081 views	Maddie 30 Jul 2010
Pinned Creating empty vector by self-ligation Started by chijing, 06 Apr 2009 1 2 3	Hot 31 replies 32,380 views	kellyhayes 23 Nov 2009
Pinned Time needed to determine G418 concentration for selection Started by Daniel5306, 13 Dec 2004	5 replies 7,594 views	Helios 10 Nov 2009
Pinned How to transfect two plasmids in a cell Started by muchmoa, 04 May 2009	10 replies 10,853 views	laurequillo 25 Sep 2009
Pinned Help with 3-way ligation Started by PhilS, 24 Feb 2009	11 replies 11,562 views	PhilS 15 Mar 2009
Pinned Old threads on Molecular Cloning are achived here Started by bioforum, 03 Feb 2009	0 replies 6,565 views	bioforum 03 Feb 2009
The right lentivirus plasmid for overexpression in human cells Started by TheFOrsh, Yesterday, 06:21 AM	1 reply 73 views	5280 Yesterday, 11:40 PM
When designing a Mammalian Expression Vector, Should you include the complete CD Started by SimoneM, 26 Feb 2015	1 reply 267 views	Smog187 26 Feb 2015
plasmid repositories Started by Chomolungma, 26 Feb 2015	0 replies 240 views	Chomolungma 26 Feb 2015
How to insert PreScission protease into a vector Started by BioExplorer, 25 Feb 2015	0 replies 271 views	BioExplorer 25 Feb 2015
Why the insert become shorter after inserting into plasmid? Started by le_duy211, 24 Feb 2015	1 reply 62 views	bob1 25 Feb 2015
Erythromycin selection of transformed e.coli Started by Meg P. Anula, 18 Feb 2015 erythromycin and 4 more...	0 replies 117 views	Meg P. Anula 18 Feb 2015
No colonies on plate after liagtion Started by KhaleesiDany, 23 Jan 2015 colonies, ligation	7 replies 599 views	KhaleesiDany 14 Feb 2015
Thermo scientific T4 DNA ligase product help Started by KhaleesiDany, 06 Feb 2015 ligation, T4 dna ligase	3 replies 138 views	KhaleesiDany 14 Feb 2015
Answered Bacteria grow on antibiotic selective plate but plasmid extracted showed no dna Started by Meg P. Anula, 09 Feb 2015 plasmid, dna insert, cloning and 2 more...	6 replies 258 views	Meg P. Anula 11 Feb 2015
Cloning a big insert in a small vector Started by PV_effect, 05 Feb 2015	2 replies 286 views	PV_effect 06 Feb 2015
Transformation E.Coli help Started by labgirl14, 03 Feb 2015	2 replies 142 views	labgirl14 03 Feb 2015
Exonucleases that won't harm circular dsDNA Started by Hehehe, 28 Jan 2015 exonuclease, ExoIII, lambda-Exo and 1 more...	1 reply 144 views	Trof 28 Jan 2015
Low Yield Miniprep Started by cytheon, 20 Jan 2015 DNA, miniprep, maxiprep	5 replies 343 views	labtastic 27 Jan 2015
Clean ligation controls but no insert Started by miST32, 22 Oct 2014	Hot 14 replies 595 views	miST32 26 Jan 2015