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Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

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Topic	Stats	Last Post Info
Pinned Sequential restriction digestion of plasmid Started by shimshady, 21 Feb 2009 1 2 3 4	Hot 58 replies 33,740 views	mahrak 23 Jun 2012
Pinned Problems with Stratagene Site Directed Mutagenesis Started by yean_ny_nie, 07 Apr 2009 1 2 3	Hot 36 replies 23,370 views	fighter 25 May 2012
Pinned ds-oligo cloning/ligation issues Started by GilsonGirl, 06 Mar 2009 1 2	Hot 18 replies 8,493 views	Trof 03 Apr 2012
Pinned What is the biggest insert for a viral vector? Started by Kami23, 06 Feb 2009 1 2	Hot 15 replies 12,131 views	wincel 24 Sep 2010
Pinned Smear in my restriction digest Started by panda, 07 May 2009	12 replies 9,781 views	wincel 24 Sep 2010
Pinned Tips on ethanol wash after nucleic acid precipitation Started by mario2004, 13 Mar 2005 1 2	Hot 22 replies 20,143 views	Maddie 30 Jul 2010
Pinned Creating empty vector by self-ligation Started by chijing, 06 Apr 2009 1 2 3	Hot 31 replies 26,644 views	kellyhayes 23 Nov 2009
Pinned Time needed to determine G418 concentration for selection Started by Daniel5306, 13 Dec 2004	5 replies 6,122 views	Helios 10 Nov 2009
Pinned How to transfect two plasmids in a cell Started by muchmoa, 04 May 2009	10 replies 9,196 views	laurequillo 25 Sep 2009
Pinned Help with 3-way ligation Started by PhilS, 24 Feb 2009	11 replies 10,018 views	PhilS 15 Mar 2009
Pinned Old threads on Molecular Cloning are achived here Started by bioforum, 03 Feb 2009	0 replies 5,039 views	bioforum 03 Feb 2009
classic cloning with restriction enzymes into empty pcDNA3.1D Started by Euterpa12, 17 May 2013 cloning, pcDNA3.1	1 reply 62 views	bob1 17 May 2013
Cloning large fragments Started by bknm, 15 May 2013 Cloning, PCR, large inserts	1 reply 81 views	Curtis 16 May 2013
Need urgent advise.. Started by M.T2013, 29 Apr 2013 cloning	3 replies 167 views	JYaron 13 May 2013
Blunt cloning - No insert, despite lots of colonies Started by rxzlmn, 06 May 2013 failed cloning	3 replies 117 views	Curtis 09 May 2013
Transformation colonies does not contain insert Started by Sandy Fowler, 08 May 2013 transformation, Ligation, Cloning	3 replies 121 views	bob1 09 May 2013
Transient Transfection Controls (Gateway Technology) Started by greggers, 15 Apr 2013 Gateway, transfection, pCR3 and 1 more...	4 replies 235 views	greggers 09 May 2013
Cloning Mitochondrial Targeting Sequence Started by speci, 27 Apr 2013	4 replies 160 views	speci 29 Apr 2013
quick change mutagenesis...... Started by kartiga natarajan, 20 Apr 2013 Help me out plz......... 1 2	Hot 15 replies 250 views	GNANA 29 Apr 2013
problem with sequencing and mapping Started by ahmadfathi, 27 Apr 2013 sequencing	0 replies 106 views	ahmadfathi 27 Apr 2013
problem in cloning Started by gdbiomed, 25 Apr 2013	2 replies 140 views	phage434 25 Apr 2013
Residues on the N terminal GST Tag Started by Pangea, 03 Jan 2013 GST TAG	1 reply 186 views	GNANA 25 Apr 2013
TA cloning: ligation problem or toxic construct? Started by DrLeo, 23 Apr 2013 TA cloning	4 replies 147 views	DrLeo 24 Apr 2013
Do I need to sequence after digestion? Started by smndtupidisaftr, 22 Apr 2013	9 replies 180 views	pito 24 Apr 2013
Reappearing band shifts/smears after double digest and gel purification Started by muLab, 23 Apr 2013 restriction, smear, NotI, SbfI and 1 more...	2 replies 156 views	muLab 23 Apr 2013