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Molecular Cloning
Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...
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Sequential restriction digestion of plasmidStarted by shimshady, 21 Feb 2009 |
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Problems with Stratagene Site Directed MutagenesisStarted by yean_ny_nie, 07 Apr 2009 |
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ds-oligo cloning/ligation issuesStarted by GilsonGirl, 06 Mar 2009 |
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What is the biggest insert for a viral vector?Started by Kami23, 06 Feb 2009 |
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Smear in my restriction digestStarted by panda, 07 May 2009 |
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Tips on ethanol wash after nucleic acid precipitationStarted by mario2004, 13 Mar 2005 |
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Creating empty vector by self-ligationStarted by chijing, 06 Apr 2009 |
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Time needed to determine G418 concentration for selectionStarted by Daniel5306, 13 Dec 2004 |
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How to transfect two plasmids in a cellStarted by muchmoa, 04 May 2009 |
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Help with 3-way ligationStarted by PhilS, 24 Feb 2009 |
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Old threads on Molecular Cloning are achived hereStarted by bioforum, 03 Feb 2009 |
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Suitable templates for in vitro transcriptionStarted by seanspotatobusiness, 06 Dec 2013 |
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Site-directed mutagenesis failedStarted by Shifting Reality, Today, 02:25 AM |
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Cloning ProblemsStarted by Apollodevi, 08 Dec 2013  cloning
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Insert & vector too dilute to perform ligationStarted by tmaz, Yesterday, 06:37 AM |
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Trouble isolating plasmid from Pseudomonas aeruginosaStarted by tihong10, 13 Nov 2013 plasmid extraction, miniprep and 1 more...
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PCR product sequencingStarted by yyc, 23 Nov 2013 |
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pcDNA 3.1 Directional TOPO clone issuesStarted by Jeremy55, 01 Dec 2013 ligation, transformation
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QIAGEN Spin Columns and Minipreps - Alternatives?!Started by Luria Bertani, 14 Aug 2012 |
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Sticky and blunt ligation issueStarted by ramblingrat, 27 Nov 2013 |
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Different ways to ensure the successful cloning?Started by tmaz, 04 Dec 2013 |
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Plasmid DNA digestion problemStarted by aum, 03 Dec 2013 |
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Handling DEPC under nitrogen/argonStarted by seanspotatobusiness, 26 Nov 2013 |
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Single codon insertStarted by ostiaziocan, 01 Dec 2013 cloning, mutagenesis
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cloning- ligationStarted by Miriam, 28 Nov 2013 |
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