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Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

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Topic	Stats	Last Post Info
Pinned Sequential restriction digestion of plasmid Started by shimshady, 21 Feb 2009 1 2 3 4	Hot 58 replies 35,341 views	mahrak 27 Aug 2013
Pinned Problems with Stratagene Site Directed Mutagenesis Started by yean_ny_nie, 07 Apr 2009 1 2 3	Hot 36 replies 26,190 views	fighter 25 May 2012
Pinned ds-oligo cloning/ligation issues Started by GilsonGirl, 06 Mar 2009 1 2	Hot 18 replies 9,081 views	Trof 03 Apr 2012
Pinned What is the biggest insert for a viral vector? Started by Kami23, 06 Feb 2009 1 2	Hot 15 replies 12,842 views	wincel 24 Sep 2010
Pinned Smear in my restriction digest Started by panda, 07 May 2009	12 replies 10,338 views	wincel 24 Sep 2010
Pinned Tips on ethanol wash after nucleic acid precipitation Started by mario2004, 13 Mar 2005 1 2	Hot 22 replies 20,909 views	Maddie 30 Jul 2010
Pinned Creating empty vector by self-ligation Started by chijing, 06 Apr 2009 1 2 3	Hot 31 replies 29,546 views	kellyhayes 23 Nov 2009
Pinned Time needed to determine G418 concentration for selection Started by Daniel5306, 13 Dec 2004	5 replies 6,515 views	Helios 10 Nov 2009
Pinned How to transfect two plasmids in a cell Started by muchmoa, 04 May 2009	10 replies 9,717 views	laurequillo 25 Sep 2009
Pinned Help with 3-way ligation Started by PhilS, 24 Feb 2009	11 replies 10,423 views	PhilS 15 Mar 2009
Pinned Old threads on Molecular Cloning are achived here Started by bioforum, 03 Feb 2009	0 replies 5,413 views	bioforum 03 Feb 2009
PCR product sequencing Started by yyc, 23 Nov 2013	3 replies 97 views	El Crazy Xabi Today, 10:11 PM
pcDNA 3.1 Directional TOPO clone issues Started by Jeremy55, 01 Dec 2013 ligation, transformation	10 replies 106 views	bob1 Today, 01:00 PM
QIAGEN Spin Columns and Minipreps - Alternatives?! Started by Luria Bertani, 14 Aug 2012	Hot 14 replies 741 views	Maxab Today, 09:39 AM
Sticky and blunt ligation issue Started by ramblingrat, 27 Nov 2013	6 replies 92 views	ramblingrat Yesterday, 10:43 AM
Different ways to ensure the successful cloning? Started by tmaz, Yesterday, 07:14 AM	2 replies 67 views	ostiaziocan Yesterday, 07:54 AM
Plasmid DNA digestion problem Started by aum, 03 Dec 2013	3 replies 57 views	phage434 03 Dec 2013
Handling DEPC under nitrogen/argon Started by seanspotatobusiness, 26 Nov 2013	3 replies 88 views	bob1 02 Dec 2013
Single codon insert Started by ostiaziocan, 01 Dec 2013 cloning, mutagenesis	3 replies 101 views	ostiaziocan 01 Dec 2013
cloning- ligation Started by Miriam, 28 Nov 2013	3 replies 85 views	Miriam 29 Nov 2013
i couldn't get the band of target gene after ligation Started by Huongnguyen.miss, 21 Nov 2013	9 replies 154 views	Huongnguyen.miss 28 Nov 2013
What is the optimal time for transfection efficiency measuring? Started by ticovka, 23 Nov 2013 transduction efficiency	1 reply 121 views	2xzwei 28 Nov 2013
Answered Incubation period after heat shock at transformation--reason? Started by luckyjade, 24 Nov 2013	3 replies 116 views	luckyjade 28 Nov 2013
Smallest mRNA length that can be translated Started by Ahrenhase, 04 Nov 2013	3 replies 153 views	bob1 25 Nov 2013
Does it make sense for snRNAs to have ribosomes bound to them? Started by Ahrenhase, 25 Nov 2013	0 replies 71 views	Ahrenhase 25 Nov 2013