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Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

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Topic	Stats	Last Post Info
Pinned Sequential restriction digestion of plasmid Started by shimshady, 21 Feb 2009 1 2 3 4	Hot 58 replies 47,826 views	mahrak 27 Aug 2013
Pinned Problems with Stratagene Site Directed Mutagenesis Started by yean_ny_nie, 07 Apr 2009 1 2 3	Hot 36 replies 36,230 views	fighter 25 May 2012
Pinned ds-oligo cloning/ligation issues Started by GilsonGirl, 06 Mar 2009 1 2	Hot 18 replies 16,933 views	Trof 03 Apr 2012
Pinned What is the biggest insert for a viral vector? Started by Kami23, 06 Feb 2009 1 2	Hot 15 replies 20,859 views	wincel 24 Sep 2010
Pinned Smear in my restriction digest Started by panda, 07 May 2009	12 replies 16,724 views	wincel 24 Sep 2010
Pinned Tips on ethanol wash after nucleic acid precipitation Started by mario2004, 13 Mar 2005 1 2	Hot 22 replies 29,481 views	Maddie 30 Jul 2010
Pinned Creating empty vector by self-ligation Started by chijing, 06 Apr 2009 1 2 3	Hot 31 replies 39,296 views	kellyhayes 23 Nov 2009
Pinned Time needed to determine G418 concentration for selection Started by Daniel5306, 13 Dec 2004	5 replies 12,390 views	Helios 10 Nov 2009
Pinned How to transfect two plasmids in a cell Started by muchmoa, 04 May 2009	10 replies 15,773 views	laurequillo 25 Sep 2009
Pinned Help with 3-way ligation Started by PhilS, 24 Feb 2009	11 replies 16,262 views	PhilS 15 Mar 2009
Pinned Old threads on Molecular Cloning are achived here Started by bioforum, 03 Feb 2009	0 replies 11,182 views	bioforum 03 Feb 2009
p1 transduction into recA- strain Started by Raygoza, 21 Sep 2017	0 replies 121 views	Raygoza 21 Sep 2017
An unusual "buffer" in a protocol for making chemically competent E.coli Started by Cistron, 06 Sep 2017 E.coli, competent cells	3 replies 307 views	labtastic 07 Sep 2017
Can someone please explain this to me? Started by Questions, 20 May 2016	2 replies 682 views	Zuzanny 28 Jul 2017
Express two different membrane proteins on the same promoter Started by anonyvous, 25 Jul 2017	1 reply 383 views	bob1 28 Jul 2017
Help with making a Dimer Started by kenny17, 09 Jul 2017 cloning, ssDNA, pGEM3zf+, MSV	0 replies 666 views	kenny17 09 Jul 2017
[Overexpression] Without upstream transcription terminator Started by lactamase, 09 May 2017	4 replies 528 views	lactamase 29 May 2017
Using modified CFP and YFP plasmids for FRET assay Started by maarts, 11 May 2017 pAquaN1, Citrine-C1, gfp, cfp and 1 more...	1 reply 738 views	labtastic 11 May 2017
Molecular cloning into pGhost5 Started by Lana, 07 Apr 2017 cloning	0 replies 962 views	Lana 07 Apr 2017
How large need homology arms to be? Started by Luca, 07 Feb 2017 CRISPR/Cas9 and 2 more...	1 reply 565 views	bob1 08 Feb 2017
Ampicillin LB agar reheating and reusing Started by Mirco G, 26 Jan 2017 antibiotics, LB, medium, agar and 1 more...	2 replies 861 views	bob1 31 Jan 2017
Using pB2GW7,0 Started by Chomolungma, 16 Jan 2017	2 replies 501 views	Chomolungma 17 Jan 2017
vector selection Started by Chomolungma, 14 Jan 2017	0 replies 402 views	Chomolungma 14 Jan 2017
propagating commercial chemically competent cells Started by merlav, 14 Dec 2016	2 replies 588 views	merlav 14 Dec 2016
After ligation,I found that my colony on amplicilin plate have not insert gene Started by sagebook, 17 Jan 2013 ligation digestion	3 replies 964 views	bob1 13 Dec 2016