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Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

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Topic	Stats	Last Post Info
Pinned Sequential restriction digestion of plasmid Started by shimshady, 21 Feb 2009 1 2 3 4	Hot 58 replies 33,746 views	mahrak 23 Jun 2012
Pinned Problems with Stratagene Site Directed Mutagenesis Started by yean_ny_nie, 07 Apr 2009 1 2 3	Hot 36 replies 23,372 views	fighter 25 May 2012
Pinned ds-oligo cloning/ligation issues Started by GilsonGirl, 06 Mar 2009 1 2	Hot 18 replies 8,495 views	Trof 03 Apr 2012
Pinned What is the biggest insert for a viral vector? Started by Kami23, 06 Feb 2009 1 2	Hot 15 replies 12,134 views	wincel 24 Sep 2010
Pinned Smear in my restriction digest Started by panda, 07 May 2009	12 replies 9,784 views	wincel 24 Sep 2010
Pinned Tips on ethanol wash after nucleic acid precipitation Started by mario2004, 13 Mar 2005 1 2	Hot 22 replies 20,146 views	Maddie 30 Jul 2010
Pinned Creating empty vector by self-ligation Started by chijing, 06 Apr 2009 1 2 3	Hot 31 replies 26,649 views	kellyhayes 23 Nov 2009
Pinned Time needed to determine G418 concentration for selection Started by Daniel5306, 13 Dec 2004	5 replies 6,123 views	Helios 10 Nov 2009
Pinned How to transfect two plasmids in a cell Started by muchmoa, 04 May 2009	10 replies 9,199 views	laurequillo 25 Sep 2009
Pinned Help with 3-way ligation Started by PhilS, 24 Feb 2009	11 replies 10,019 views	PhilS 15 Mar 2009
Pinned Old threads on Molecular Cloning are achived here Started by bioforum, 03 Feb 2009	0 replies 5,041 views	bioforum 03 Feb 2009
Cloning advice Started by weilun, Today, 12:24 AM stop codon	1 reply 40 views	aimikins Today, 01:41 PM
"Easiest" sticky end combination Started by doxorubicin, Today, 03:45 AM ligation, cloning and 1 more...	2 replies 30 views	GNANA Today, 06:29 AM
classic cloning with restriction enzymes into empty pcDNA3.1D Started by Euterpa12, 17 May 2013 cloning, pcDNA3.1	1 reply 71 views	bob1 17 May 2013
Cloning large fragments Started by bknm, 15 May 2013 Cloning, PCR, large inserts	1 reply 86 views	Curtis 16 May 2013
Need urgent advise.. Started by M.T2013, 29 Apr 2013 cloning	3 replies 172 views	JYaron 13 May 2013
Blunt cloning - No insert, despite lots of colonies Started by rxzlmn, 06 May 2013 failed cloning	3 replies 119 views	Curtis 09 May 2013
Transformation colonies does not contain insert Started by Sandy Fowler, 08 May 2013 transformation, Ligation, Cloning	3 replies 124 views	bob1 09 May 2013
Transient Transfection Controls (Gateway Technology) Started by greggers, 15 Apr 2013 Gateway, transfection, pCR3 and 1 more...	4 replies 238 views	greggers 09 May 2013
Cloning Mitochondrial Targeting Sequence Started by speci, 27 Apr 2013	4 replies 163 views	speci 29 Apr 2013
quick change mutagenesis...... Started by kartiga natarajan, 20 Apr 2013 Help me out plz......... 1 2	Hot 15 replies 252 views	GNANA 29 Apr 2013
problem with sequencing and mapping Started by ahmadfathi, 27 Apr 2013 sequencing	0 replies 107 views	ahmadfathi 27 Apr 2013
problem in cloning Started by gdbiomed, 25 Apr 2013	2 replies 142 views	phage434 25 Apr 2013
Residues on the N terminal GST Tag Started by Pangea, 03 Jan 2013 GST TAG	1 reply 187 views	GNANA 25 Apr 2013
TA cloning: ligation problem or toxic construct? Started by DrLeo, 23 Apr 2013 TA cloning	4 replies 148 views	DrLeo 24 Apr 2013