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Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

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Topic	Stats	Last Post Info
Pinned Sequential restriction digestion of plasmid Started by shimshady, 21 Feb 2009 1 2 3 4	Hot 58 replies 39,447 views	mahrak 27 Aug 2013
Pinned Problems with Stratagene Site Directed Mutagenesis Started by yean_ny_nie, 07 Apr 2009 1 2 3	Hot 36 replies 28,865 views	fighter 25 May 2012
Pinned ds-oligo cloning/ligation issues Started by GilsonGirl, 06 Mar 2009 1 2	Hot 18 replies 10,822 views	Trof 03 Apr 2012
Pinned What is the biggest insert for a viral vector? Started by Kami23, 06 Feb 2009 1 2	Hot 15 replies 14,745 views	wincel 24 Sep 2010
Pinned Smear in my restriction digest Started by panda, 07 May 2009	12 replies 11,532 views	wincel 24 Sep 2010
Pinned Tips on ethanol wash after nucleic acid precipitation Started by mario2004, 13 Mar 2005 1 2	Hot 22 replies 22,957 views	Maddie 30 Jul 2010
Pinned Creating empty vector by self-ligation Started by chijing, 06 Apr 2009 1 2 3	Hot 31 replies 32,230 views	kellyhayes 23 Nov 2009
Pinned Time needed to determine G418 concentration for selection Started by Daniel5306, 13 Dec 2004	5 replies 7,533 views	Helios 10 Nov 2009
Pinned How to transfect two plasmids in a cell Started by muchmoa, 04 May 2009	10 replies 10,786 views	laurequillo 25 Sep 2009
Pinned Help with 3-way ligation Started by PhilS, 24 Feb 2009	11 replies 11,491 views	PhilS 15 Mar 2009
Pinned Old threads on Molecular Cloning are achived here Started by bioforum, 03 Feb 2009	0 replies 6,507 views	bioforum 03 Feb 2009
No colonies on plate after liagtion Started by KhaleesiDany, 23 Jan 2015 colonies, ligation	1 reply 457 views	CPRES 28 Jan 2015
Exonucleases that won't harm circular dsDNA Started by Hehehe, 28 Jan 2015 exonuclease, ExoIII, lambda-Exo and 1 more...	1 reply 74 views	Trof 28 Jan 2015
Low Yield Miniprep Started by cytheon, 20 Jan 2015 DNA, miniprep, maxiprep	5 replies 238 views	labtastic 27 Jan 2015
Clean ligation controls but no insert Started by miST32, 22 Oct 2014	Hot 14 replies 554 views	miST32 26 Jan 2015
How to produce transgenic mice? Started by Curtis, 24 Jan 2015	10 replies 145 views	Curtis 26 Jan 2015
low DNA yield after DNA digestion and gel extraction Started by KhaleesiDany, 23 Jan 2015 Digestion, Dna yield and 1 more...	0 replies 379 views	KhaleesiDany 23 Jan 2015
white-green selection problems Started by mark.ibt, 22 Jan 2015	0 replies 394 views	mark.ibt 22 Jan 2015
troubleshooting for a simple SDM Started by Biogareth, 31 Dec 2014 mutagenesis	2 replies 210 views	labtastic 22 Jan 2015
Question: Can I cut out rb glob PA Terminator Started by Fabian Yu, 21 Jan 2015 cloning, dna, plasmid and 1 more...	1 reply 341 views	CPRES 21 Jan 2015
using CRISPR to introduce a single nucleotide change Started by Tszki, 20 Jan 2015 CRISPR	0 replies 452 views	Tszki 20 Jan 2015
Cloning problem, sticky - blunt ends ligation fail . Started by Danbas28, 12 Feb 2013 blunt ends ligation and 2 more...	6 replies 511 views	Juanita Mathews 17 Jan 2015
Answered question about Clonetech Retro-X Tet-on system Started by jackster101, 12 Jan 2015 inducible system	3 replies 419 views	jackster101 14 Jan 2015
Clonetech Retro-X Tet-on system Started by jackster101, 12 Jan 2015 inducible system	0 replies 979 views	jackster101 12 Jan 2015
quiaquick gel extraction troubleshooting Started by labgirl14, 12 Jan 2015	1 reply 139 views	CPRES 12 Jan 2015