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Molecular Cloning
Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...
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Sequential restriction digestion of plasmidStarted by shimshady, 21 Feb 2009 |
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Problems with Stratagene Site Directed MutagenesisStarted by yean_ny_nie, 07 Apr 2009 |
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ds-oligo cloning/ligation issuesStarted by GilsonGirl, 06 Mar 2009 |
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What is the biggest insert for a viral vector?Started by Kami23, 06 Feb 2009 |
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Smear in my restriction digestStarted by panda, 07 May 2009 |
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Tips on ethanol wash after nucleic acid precipitationStarted by mario2004, 13 Mar 2005 |
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Creating empty vector by self-ligationStarted by chijing, 06 Apr 2009 |
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Time needed to determine G418 concentration for selectionStarted by Daniel5306, 13 Dec 2004 |
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How to transfect two plasmids in a cellStarted by muchmoa, 04 May 2009 |
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Help with 3-way ligationStarted by PhilS, 24 Feb 2009 |
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Old threads on Molecular Cloning are achived hereStarted by bioforum, 03 Feb 2009 |
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Vector-insert ratioStarted by Raygoza, 26 Jul 2014 |
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Cloning contaminationStarted by Clare, 25 Jul 2014 |
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Blunt end cloningStarted by Clare, 19 Jun 2014  help please!
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My battle with BamHIStarted by georgiadave, 23 Mar 2010 |
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plasmid constructionStarted by ms student, 24 Jul 2014 |
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primer design with restriction enzymeStarted by SF_HK, 22 Jul 2014 bases flanking restricti
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Restriction to remove backgroundStarted by Raygoza, 18 Jul 2014 |
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vector propagation in E coliStarted by plasmamembrane, 19 Jul 2014 |
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How to subclone FoxO fusion protein expression plasmid into pDsRed1N1 vectorStarted by nrl81182, 18 Jul 2014 |
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tn5 transposonStarted by Raygoza, 18 Jul 2014 |
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Restriction and ligation time intervalStarted by Raygoza, 11 Jul 2014 ligation, restriction
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Lentivirus copy numberStarted by Zduan, 08 Jul 2014 lentivirus, experimental design and 3 more...
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Problem shrna cloning, bad sequencing resultsStarted by Finouu0106, 09 Jul 2014 shrna, cloning, sequencing
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5' RLM RACEStarted by Epigeneticist, 09 Jul 2014 TOTAL RNA, PCR, RNA PURIFICATION and 1 more...
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