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Molecular Cloning
Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...
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Sequential restriction digestion of plasmidStarted by shimshady, 21 Feb 2009 |
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Problems with Stratagene Site Directed MutagenesisStarted by yean_ny_nie, 07 Apr 2009 |
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ds-oligo cloning/ligation issuesStarted by GilsonGirl, 06 Mar 2009 |
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What is the biggest insert for a viral vector?Started by Kami23, 06 Feb 2009 |
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Smear in my restriction digestStarted by panda, 07 May 2009 |
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Tips on ethanol wash after nucleic acid precipitationStarted by mario2004, 13 Mar 2005 |
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Creating empty vector by self-ligationStarted by chijing, 06 Apr 2009 |
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Time needed to determine G418 concentration for selectionStarted by Daniel5306, 13 Dec 2004 |
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How to transfect two plasmids in a cellStarted by muchmoa, 04 May 2009 |
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Help with 3-way ligationStarted by PhilS, 24 Feb 2009 |
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Old threads on Molecular Cloning are achived hereStarted by bioforum, 03 Feb 2009 |
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Help with making a DimerStarted by kenny17, 09 Jul 2017  cloning, ssDNA, pGEM3zf+, MSV
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[Overexpression] Without upstream transcription terminatorStarted by lactamase, 09 May 2017 |
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![[Overexpression] Without upstream transcription terminator - last post by lactamase](http://www.gravatar.com/avatar/ed95266ad5fa49215efc400961aeb7f9?s=100&d=http%3A%2F%2Fwww.protocol-online.org%2Fforums%2F%2Fpublic%2Fstyle_images%2Fmaster%2Fprofile%2Fdefault_large.png)
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Using modified CFP and YFP plasmids for FRET assayStarted by maarts, 11 May 2017 pAquaN1, Citrine-C1, gfp, cfp and 1 more...
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Molecular cloning into pGhost5Started by Lana, 07 Apr 2017 cloning
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How large need homology arms to be?Started by Luca, 07 Feb 2017 CRISPR/Cas9 and 2 more...
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Ampicillin LB agar reheating and reusingStarted by Mirco G, 26 Jan 2017 antibiotics, LB, medium, agar and 1 more...
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Using pB2GW7,0Started by Chomolungma, 16 Jan 2017 |
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vector selectionStarted by Chomolungma, 14 Jan 2017 |
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propagating commercial chemically competent cellsStarted by merlav, 14 Dec 2016 |
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After ligation,I found that my colony on amplicilin plate have not insert geneStarted by sagebook, 17 Jan 2013 ligation digestion
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In-fusion HD cloningStarted by DrLeo, 30 May 2013 |
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m13 blue white screeningStarted by Paula N, 03 Oct 2016 |
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Plasmid purification for Gibson AssemblyStarted by Yu Zhang, 09 Aug 2016 plasmid, purification and 1 more...
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TA cloning..Urgent Help!!Started by Mehjabeen, 07 Jun 2016 |
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