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Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

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Topic	Stats	Last Post Info
Pinned Sequential restriction digestion of plasmid Started by shimshady, 21 Feb 2009 1 2 3 4	Hot 58 replies 49,889 views	mahrak 27 Aug 2013
Pinned Problems with Stratagene Site Directed Mutagenesis Started by yean_ny_nie, 07 Apr 2009 1 2 3	Hot 36 replies 37,938 views	fighter 25 May 2012
Pinned ds-oligo cloning/ligation issues Started by GilsonGirl, 06 Mar 2009 1 2	Hot 18 replies 18,398 views	Trof 03 Apr 2012
Pinned What is the biggest insert for a viral vector? Started by Kami23, 06 Feb 2009 1 2	Hot 15 replies 22,311 views	wincel 24 Sep 2010
Pinned Smear in my restriction digest Started by panda, 07 May 2009	12 replies 18,029 views	wincel 24 Sep 2010
Pinned Tips on ethanol wash after nucleic acid precipitation Started by mario2004, 13 Mar 2005 1 2	Hot 22 replies 31,018 views	Maddie 30 Jul 2010
Pinned Creating empty vector by self-ligation Started by chijing, 06 Apr 2009 1 2 3	Hot 31 replies 40,992 views	kellyhayes 23 Nov 2009
Pinned Time needed to determine G418 concentration for selection Started by Daniel5306, 13 Dec 2004	5 replies 13,632 views	Helios 10 Nov 2009
Pinned How to transfect two plasmids in a cell Started by muchmoa, 04 May 2009	9 replies 17,020 views	laurequillo 25 Sep 2009
Pinned Help with 3-way ligation Started by PhilS, 24 Feb 2009	11 replies 17,531 views	PhilS 15 Mar 2009
Pinned Old threads on Molecular Cloning are achived here Started by bioforum, 03 Feb 2009	0 replies 12,359 views	bioforum 03 Feb 2009
Plasmid dna size Started by Angeline, 01 Dec 2017	3 replies 523 views	OldCloner 13 Jun 2018
Appearance of a higher molecular weight band after plasmid isolation? (NOT chros Started by DaRQsiDe, 02 Jan 2014 Plasmid isolation and 2 more...	1 reply 902 views	OldCloner 13 Jun 2018
Plasmid analysis in limited conditions Started by Trof, 02 May 2018	4 replies 240 views	Trof 25 May 2018
Answered Neomycin/Kanamycin Resistant gene Started by Thomson, 10 May 2018 neomycin, kanamycin and 1 more...	2 replies 189 views	Thomson 13 May 2018
Why do I get two bands on the gel of a digested PCR product after purification w Started by Delimar Rodriguez, 27 Apr 2018 molecular, cloning, digest, pcr and 1 more...	0 replies 389 views	Delimar Rodriguez 27 Apr 2018
Transformation Started by D.Luffy, 24 Apr 2018	2 replies 204 views	D.Luffy 27 Apr 2018
Troubleshooting with restriction enzymes during cloning Started by liligompac, 25 Apr 2018 cloning, pgem t easy and 2 more...	4 replies 438 views	liligompac 26 Apr 2018
Plasmid distributors for Luciferase Assay? Started by Helder_90, 03 Apr 2018	2 replies 198 views	Helder_90 03 Apr 2018
Answered Ligate a very small insert into a vector Started by Thomson, 13 Mar 2018 insert, small dna fragment and 2 more...	3 replies 334 views	bob1 14 Mar 2018
Restriction enzyme Started by Huongnguyen.miss, 20 Dec 2017	1 reply 660 views	bob1 20 Dec 2017
Determining optimal digestion time for partial digestion Started by dentategyrus, 18 Oct 2011	1 reply 972 views	Zagami Francesco 29 Oct 2017
p1 transduction into recA- strain Started by Raygoza, 21 Sep 2017	0 replies 807 views	Raygoza 21 Sep 2017
An unusual "buffer" in a protocol for making chemically competent E.coli Started by Cistron, 06 Sep 2017 E.coli, competent cells	3 replies 663 views	labtastic 07 Sep 2017
Can someone please explain this to me? Started by Questions, 20 May 2016	2 replies 978 views	Zuzanny 28 Jul 2017