Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

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Topic	Stats	Last Post Info
Pinned Problems with Stratagene Site Directed Mutagenesis Started by yean_ny_nie, 07 Apr 2009 1 2 3	Hot 31 replies 15,745 views	elidamar 24 Jan 2012
Pinned ds-oligo cloning/ligation issues Started by GilsonGirl, 06 Mar 2009	10 replies 6,377 views	Toyitta 18 Nov 2011
Pinned Sequential restriction digestion of plasmid Started by shimshady, 21 Feb 2009 1 2 3 4	Hot 52 replies 28,246 views	ampelo 01 Jun 2011
Pinned What is the biggest insert for a viral vector? Started by Kami23, 06 Feb 2009 1 2	Hot 15 replies 9,876 views	wincel 24 Sep 2010
Pinned Smear in my restriction digest Started by panda, 07 May 2009	12 replies 7,824 views	wincel 24 Sep 2010
Pinned Creating empty vector by self-ligation Started by chijing, 06 Apr 2009 1 2 3	Hot 31 replies 18,989 views	kellyhayes 23 Nov 2009
Pinned Time needed to determine G418 concentration for selection Started by Daniel5306, 13 Dec 2004	5 replies 4,915 views	Helios 10 Nov 2009
Pinned How to transfect two plasmids in a cell Started by muchmoa, 04 May 2009	10 replies 7,301 views	laurequillo 25 Sep 2009
Pinned Help with 3-way ligation Started by PhilS, 24 Feb 2009	11 replies 8,571 views	PhilS 15 Mar 2009
Pinned Old threads on Molecular Cloning are achived here Started by bioforum, 03 Feb 2009	0 replies 4,073 views	bioforum 03 Feb 2009
overlapping PCR protocol Started by dan_, Yesterday, 06:08 AM	0 replies 31 views	dan_ Yesterday, 06:08 AM
why my solution II turns cloudy during plasmid extraction? Started by Curtis, 04 Feb 2012	6 replies 85 views	Curtis 08 Feb 2012
screening of positive clones Started by novagen, 12 Feb 2010	5 replies 1,221 views	Sandy143 08 Feb 2012
Cloning two genes in the same plasmid Started by saad, 06 Feb 2012	4 replies 56 views	saad 07 Feb 2012
Ligation gel picture interpretation Started by HOYAJM, 01 Feb 2012	4 replies 84 views	HOYAJM 06 Feb 2012
cloning an AT rich gene Started by nahlagadalla, 06 Feb 2012	3 replies 41 views	HOYAJM 06 Feb 2012
How to wipe out fragment of dead cells from the suspension cells? Started by hshzhq, 05 Feb 2012 suspension cell, cell fragment	1 reply 54 views	scolix 06 Feb 2012
Problem: Cloning DNA shuffled band Started by OA17, 03 Feb 2012 DNA shuffling, cloning	2 replies 46 views	OA17 04 Feb 2012
strange subcloning problem Started by miclee0710, 01 Feb 2012 ligation, cloning	8 replies 109 views	phage434 02 Feb 2012
Enzymetic digestion with ApaI and HincII Started by syed.ibrahim, 02 Feb 2012	2 replies 67 views	scolix 02 Feb 2012
shRNA to primary keratinocytes Started by EvilTwin, 02 Feb 2012 transfection, transduction, shRNA	1 reply 75 views	scolix 02 Feb 2012
Ligation of insert to itself problem Started by precip_man, 30 Jan 2012	3 replies 101 views	scolix 01 Feb 2012
Recombination due to overselective plate? Started by Trof, 19 Jan 2012	11 replies 224 views	Trof 01 Feb 2012
Cloned sequence is not predicted size by western blot Started by Rara, 31 Jan 2012 Cloning, Western blot, Kilodalton	1 reply 79 views	scolix 01 Feb 2012
Trouble cloning E.coli cells with pUC18 and insert Started by Biol74, 31 Jan 2012 electroporation, competent, MKH13	0 replies 67 views	Biol74 31 Jan 2012