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Biochemistry

Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

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Topic	Stats	Last Post Info
Preparing Palmitate:BSA solution for treatment of cells? Started by pobrien, 28 Mar 2012 palmitate, palmitic acid, PA:BSA	7 replies 966 views	nickwaal 02 Jul 2012
freeze dried powder Started by Sid, 14 May 2012 powdered, mushroom, freeze-dried	7 replies 346 views	mdfenko 16 May 2012
Molar calculation from liquid Started by chandch, 30 Nov 2012 molarity	7 replies 421 views	chandch 01 Dec 2012
Identifying new Ca2+-binding proteins Started by cardosopedro, 10 May 2013	7 replies 128 views	cardosopedro 14 May 2013
Urea and protein storage Started by bachai, 15 Mar 2009	6 replies 3,604 views	mdfenko 17 Mar 2009
how to prepare rnase? Started by sagar, 01 Apr 2009	6 replies 3,548 views	sagar 04 Apr 2009
Extinction coefficient Started by rajaram, 08 May 2009	6 replies 6,553 views	rajaram 12 May 2009
lowry method Started by ujla80, 25 Jun 2009	6 replies 3,331 views	ujla80 26 Jun 2009
DTNB (Ellman's reagent) Started by kamilaf, 01 Oct 2009	6 replies 4,501 views	kamilaf 13 Oct 2009
Blocking buffer for antibodies generated in goat. Started by bioxesus, 08 Oct 2009	6 replies 4,886 views	klinmed 14 Oct 2009
low dead volume Started by kentz19, 13 Jan 2010	6 replies 1,742 views	Gerard 22 Jun 2010
calculating Ki from IC50 Started by kamilaf, 12 Feb 2010	6 replies 3,114 views	CAT 13 Jul 2010
Quantification of peptide Total GIP Started by Anisha Kharkia, 23 Feb 2011	6 replies 669 views	mdfenko 28 Feb 2011
Atpase activity Started by rimal, 01 May 2011	6 replies 1,049 views	mdfenko 06 May 2011
Western Blot no signal Started by gorkin, 16 Aug 2011 Western Blot	6 replies 1,385 views	gorkin 17 Aug 2011
Help with Ethidium Bromide Contamination Started by RJ1989, 15 Nov 2011 Ethidium Bromide, etbr and 2 more...	6 replies 2,449 views	mdfenko 22 Nov 2011
How to prepare Tris-HCl buffer for protein crystallization? What does 33mM mean? Started by spatr, 20 Mar 2012 tris, buffer	6 replies 733 views	mdfenko 26 Mar 2012
Trouble Shooting Zymogram Started by Laura Daniel, 02 Aug 2012 zymogram, MMP-2, MMP-9	6 replies 290 views	Laura Daniel 06 Aug 2012
Conversion of Absorbance into enzyme units Started by San1986, 05 Aug 2012	6 replies 259 views	San1986 09 Aug 2012
NADPH monitoring Started by cardosopedro, 22 Apr 2013	6 replies 155 views	cardosopedro 23 Apr 2013
Maleimide chemsitry with thiols Started by wildcat, 13 Feb 2009	5 replies 6,627 views	celerychem 05 Jan 2010
Detecting glucose in reactions with OD Started by longer, 16 Mar 2009	5 replies 3,114 views	longer 23 Mar 2009
FPLC vs. HPLC Started by parkc23, 30 Jun 2009	5 replies 8,349 views	T C 09 Jul 2009
Making ampicillin solution Started by miraclestrain, 23 Jul 2009	5 replies 9,379 views	phage434 28 Jul 2009
biochemistry of H1N1 virus Started by ujla80, 06 Aug 2009	5 replies 9,410 views	bob1 23 Aug 2009