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Biochemistry

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Topic	Stats	Last Post Info
Protein fragment is alwasy degraded post transfection 48 hrs Started by lilin_hust, 09 Mar 2014 protein degraded	1 reply 47 views	jerryshelly1 10 Mar 2014
40mM EDTA in (non-metallo)protease assay buffer Started by DRT, 05 Mar 2014	2 replies 79 views	DRT 06 Mar 2014
What might cause 17 Dalton loss in MW of my peptide? Started by ionchannelbk, 03 Mar 2014 Peptide, Protein, Stability and 1 more...	0 replies 73 views	ionchannelbk 03 Mar 2014
Salt in medium Started by johnuknow, 26 Feb 2014 salt in medium	3 replies 105 views	mdfenko 26 Feb 2014
metalloproteinase 2 handling Started by claire84, 24 Feb 2014	1 reply 85 views	bob1 24 Feb 2014
Adenosine Deaminase assay Started by srimadh, 22 Feb 2014 adenosine, ada and 3 more...	0 replies 110 views	srimadh 22 Feb 2014
Troubleshooting Cell Surface Biotinylation Started by electromagnetic32, 19 Feb 2014 biotinylation	0 replies 99 views	electromagnetic32 19 Feb 2014
Measuring Fe(IV) concentration in solution? Started by stellaparallax, 22 Jan 2014	1 reply 103 views	El Crazy Xabi 22 Jan 2014
Buffer Substitution: Calcium for Magnesium Started by hotstuffdb22, 04 Jan 2014 buffer, magnesium, calcium	4 replies 262 views	hotstuffdb22 18 Jan 2014
Histone extraction from nuclear fraction - Acid or salt Started by LacquerHead, 28 Dec 2013	0 replies 186 views	$Histone extraction from nuclear fraction - Acid or salt - last post by LacquerHead$ LacquerHead 28 Dec 2013
Help need to determine enzyme assay to detect plant growth Started by ckzimase, 26 Dec 2013 enzyme assay, plant growth	0 replies 158 views	ckzimase 26 Dec 2013
MgCl2 (and EDTA) in buffers Started by schaapje_86, 19 Apr 2012 MgCl2, EDTA, proteins	10 replies 1,161 views	mdfenko 14 Dec 2013
Binding affinity testing by fluorescence titration Started by Biogareth, 13 Dec 2013	1 reply 275 views	Biogareth 14 Dec 2013
flag tag Started by M.B.T, 11 Dec 2013 tag, flag N-terminus	2 replies 180 views	mdfenko 12 Dec 2013
Measurement of deferoxamine mesylate concentration Started by stellaparallax, 05 Dec 2013	0 replies 179 views	stellaparallax 05 Dec 2013
Conversion of units please help..for IC50 Started by Praitibha Batra, 06 Oct 2013 BIOCHEMISTRY	4 replies 361 views	Praitibha Batra 30 Oct 2013
cell culture sample preparation for HPLC Started by janap, 09 Oct 2013	0 replies 240 views	janap 09 Oct 2013
corticosterone extraction from rat faecal pellets Started by dharanimahesh, 08 Oct 2013 corticosterone	0 replies 258 views	dharanimahesh 08 Oct 2013
Answered 25 mm HEPES/NH4OH (pH 8.0) Started by FreddieP, 02 Oct 2013 biochemistry	2 replies 262 views	FreddieP 03 Oct 2013
Procyanidin b2 sensitivity Started by Rebirth, 02 Oct 2013	2 replies 156 views	Rebirth 02 Oct 2013
Recombinant expression of cytokines in mammalian cells without the propeptide re Started by guyava, 23 Jul 2013 propeptide, cytokines and 3 more...	1 reply 334 views	dimensionsbio 28 Sep 2013
What unit of enzyme amount is Vmax standardized to? Started by Ahrenhase, 22 Sep 2013	3 replies 208 views	bob1 26 Sep 2013
Growing cells in presence of substrate Started by Kmu, 25 Sep 2013 protein expression, phosphate	0 replies 265 views	Kmu 25 Sep 2013
Nuclei preparation for nuclear RNA extraction Started by NemaToStella, 23 Sep 2013	0 replies 212 views	NemaToStella 23 Sep 2013
Elution of biotonylated proteins Started by jameshall1384, 18 Sep 2013 DTT Biotin	0 replies 217 views	jameshall1384 18 Sep 2013