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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,885 topics
  • 6,016 replies
Any suggestion for SDS-PAGE... - last post by biomart

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 634 topics
  • 1,179 replies
Different Accession numbers - last post by Mad Researcher


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 500 topics
  • 1,050 replies
why a protein domain fold i... - last post by bob1

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 135 topics
  • 348 replies
What gene to insert in my a... - last post by Curtis

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,222 topics
  • 3,850 replies
The adipose cell culturing - last post by bob1

Tissue and Cell Culture

  • 1,768 topics
  • 3,928 replies
Problem with MDA-MB-468 cul... - last post by isolda

Stem Cell

  • 138 topics
  • 211 replies
Stem Cell Donation - last post by Chloe Mica

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 416 topics
  • 851 replies
Hoechst33342/7-AAD staining - last post by TinaTeaspoon

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,198 topics
  • 13,054 replies
SYBR Green Real time PCR - last post by phage434

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,942 topics
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Lower Plasmid Yields - last post by BMF

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,293 topics
  • 6,318 replies
qPCR error bars - confused! - last post by miRman

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 653 topics
  • 1,357 replies
Mature miRNA in expression... - last post by mohsen222

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,082 topics
  • 4,068 replies
Liquid nitrogen protein ext... - last post by phage434

Protein Expression and Purification

  • 780 topics
  • 1,930 replies
Cant seem to detect HA-tagg... - last post by KhaleesiDany

SDS-PAGE and Western Blotting

  • 1,167 topics
  • 3,966 replies
ECM1 on a western blot, why... - last post by bob1


Discussions on antibody, immunohistochemistry and more...

  • 1,652 topics
  • 2,713 replies
Question about ELISA assay - last post by Venus Timberlake


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,068 topics
  • 3,142 replies
Where can I read up if cert... - last post by tretol

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 214 topics
  • 341 replies
Amplification problem in DN... - last post by sherrycd

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 615 topics
  • 1,656 replies
bisulfte conversion with Am... - last post by hiri

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 340 topics
  • 980 replies
How many NGS readings for a... - last post by Trof

Macroarray and Microarray

Topics related to different array techniques

  • 128 topics
  • 232 replies
Microarray resources - last post by mdfenko


Questions related to neuroscience and other neuro-related fields

  • 191 topics
  • 265 replies
Why GFP/YFP in neurons is l... - last post by Marinavv

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 182 topics
  • 416 replies
Routine medical tests on mi... - last post by pito

Botany and Plant Biology

Topics specific to plants.

  • 113 topics
  • 270 replies
Plant hosting of Pseudomona... - last post by Phil Pense

Pharmacology and Pharmaceutics

  • 80 topics
  • 151 replies
Calculating Drug concentrat... - last post by murusa

Histology and Pathology

  • 143 topics
  • 197 replies
Primary antibody too concen... - last post by mdfenko


  • 33 topics
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Freezing PBMCs that had bee... - last post by Trof


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5 M NaCl stock - last post by Arslan Iftikhar

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