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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,962 topics
  • 6,157 replies
How to deal with delta abso... - last post by djw23

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 656 topics
  • 1,199 replies
MIT cancer course - last post by Bella Smith


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 524 topics
  • 1,114 replies
Help with isolating protein... - last post by molbiolnew

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 141 topics
  • 360 replies
How do we know a study is e... - last post by hobglobin

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,245 topics
  • 3,873 replies
When overexpressing several... - last post by Lizzyf23

Tissue and Cell Culture

  • 1,820 topics
  • 4,008 replies
Monitoring Oxygen - last post by squallweathered

Stem Cell

  • 139 topics
  • 211 replies
Cryopreserving human ES dir... - last post by neuropath

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 428 topics
  • 863 replies
Correct filter - last post by Arsh

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,274 topics
  • 13,162 replies
Transfection Vs Transduction - last post by Mad Researcher

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,977 topics
  • 6,504 replies
Restriction enzyme - last post by bob1

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,349 topics
  • 6,415 replies
PCR is inconsistent - last post by Mad Researcher

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 664 topics
  • 1,387 replies
We need a way to see microR... - last post by Fredreload

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,124 topics
  • 4,171 replies
coIP troubleshooting - last post by Trof

Protein Expression and Purification

  • 820 topics
  • 2,034 replies
S. aureus lysis protocol fo... - last post by firefly2280

SDS-PAGE and Western Blotting

  • 1,203 topics
  • 4,048 replies
Sample buffer - last post by bob1


Discussions on antibody, immunohistochemistry and more...

  • 1,676 topics
  • 2,771 replies
ELISA positive result from... - last post by bob1


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,103 topics
  • 3,203 replies
bacterial culture not pelle... - last post by bob1

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 225 topics
  • 347 replies
TCGA-the cancer genome atlas - last post by bob1

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 622 topics
  • 1,667 replies
Bisulfite conversion, PCR e... - last post by mavpouck2

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 349 topics
  • 989 replies
Variable ChiP-qPCR even in... - last post by Sandeep Gupta

Macroarray and Microarray

Topics related to different array techniques

  • 129 topics
  • 234 replies
online microarray data - last post by LizRepositive


Questions related to neuroscience and other neuro-related fields

  • 194 topics
  • 269 replies
How do photoreceptors disti... - last post by Trof

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 185 topics
  • 419 replies
Is it possible to cause mor... - last post by molbiolnew

Botany and Plant Biology

Topics specific to plants.

  • 117 topics
  • 281 replies
In vitro culture saffron - last post by mdfenko

Pharmacology and Pharmaceutics

  • 84 topics
  • 164 replies
Crosslinking with glutarald... - last post by labtastic

Histology and Pathology

  • 160 topics
  • 206 replies
Intestine tissue falling of... - last post by Rnotk


  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof


  • 62 topics
  • 160 replies
solid phase extraction cort... - last post by mdfenko

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