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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,994 topics
  • 6,220 replies
Preventing contamination in... - last post by merlav

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 664 topics
  • 1,214 replies
Problem managing big table... - last post by bob1


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 527 topics
  • 1,126 replies
activity optimalisation of... - last post by OldCloner

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 144 topics
  • 362 replies
New generation "vaccine... - last post by henrilentonen

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,256 topics
  • 3,884 replies
Co-localisation Analysis -... - last post by bob1

Tissue and Cell Culture

  • 1,839 topics
  • 4,035 replies
Microisland formation - last post by Mad Researcher

Stem Cell

  • 139 topics
  • 211 replies
Cryopreserving human ES dir... - last post by neuropath

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 435 topics
  • 867 replies
Two distinct populations ob... - last post by OldCloner

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,293 topics
  • 13,198 replies
stable transfection - last post by SF_HK

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,986 topics
  • 6,537 replies
low copy number plasmid pur... - last post by Curtis

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,370 topics
  • 6,468 replies
How to publish real time PC... - last post by bob1

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 666 topics
  • 1,388 replies
miRNA isolation from serum - last post by seomelon06

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,134 topics
  • 4,179 replies
blood contamination - last post by Zagami Francesco

Protein Expression and Purification

  • 828 topics
  • 2,053 replies
How to obtain a non-cellula... - last post by Trof

SDS-PAGE and Western Blotting

  • 1,213 topics
  • 4,079 replies
Can anyone help me with Wes... - last post by Vui


Discussions on antibody, immunohistochemistry and more...

  • 1,686 topics
  • 2,774 replies
Virus neutralization calcul... - last post by bob1


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,121 topics
  • 3,253 replies
Sudden growth on streak plates - last post by bob1

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 230 topics
  • 350 replies
molecular biology - last post by mdfenko

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 624 topics
  • 1,667 replies
"Binomial" and its... - last post by MindlessJPEG

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 349 topics
  • 989 replies
Variable ChiP-qPCR even in... - last post by Sandeep Gupta

Macroarray and Microarray

Topics related to different array techniques

  • 130 topics
  • 235 replies
Searching for Agilent G2505... - last post by mdfenko


Questions related to neuroscience and other neuro-related fields

  • 194 topics
  • 269 replies
How do photoreceptors disti... - last post by Trof

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 185 topics
  • 419 replies
Is it possible to cause mor... - last post by molbiolnew

Botany and Plant Biology

Topics specific to plants.

  • 120 topics
  • 283 replies
3D seed shape (like a taper... - last post by paulindr

Pharmacology and Pharmaceutics

  • 84 topics
  • 164 replies
Crosslinking with glutarald... - last post by labtastic

Histology and Pathology

  • 169 topics
  • 215 replies
Literature Review Suggestions - last post by dawudhilli


  • 33 topics
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Freezing PBMCs that had bee... - last post by Trof


  • 65 topics
  • 170 replies
Water Treatment Process (la... - last post by mdfenko

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