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Protocols and Techniques Forums


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General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,772 topics
  • 5,722 replies
Final % DMSO Concentration - last post by Fu Shun Wong

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 589 topics
  • 1,119 replies
weblogo problem - last post by Soumble

Biochemistry

Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 471 topics
  • 983 replies
How to determine low Km val... - last post by DRT

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 119 topics
  • 316 replies
Paper-roll Chromatography/E... - last post by 27thcentury

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,170 topics
  • 3,769 replies
Does Opti-mem contain serum - last post by Tabaluga

Tissue and Cell Culture

  • 1,648 topics
  • 3,727 replies
Expired medium - last post by bob1

Stem Cell

  • 134 topics
  • 200 replies
Totipotent Stem Cell From M... - last post by caters

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 389 topics
  • 817 replies
Cell Cycle Analysis - cruci... - last post by bob1

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,035 topics
  • 12,742 replies
Visualizing GFP in transgen... - last post by astroboy89

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,835 topics
  • 6,119 replies
ratios insert/vector - last post by lucilius

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,163 topics
  • 6,030 replies
3' RACE  troubles - last post by sezerf

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 622 topics
  • 1,321 replies
BLOCK-iT Pol II miR RNAi sy... - last post by bob1

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,029 topics
  • 3,942 replies
MAPK/MEK Inhibitor stored i... - last post by matthew.summers

Protein Expression and Purification

  • 729 topics
  • 1,804 replies
EGFP expression - last post by labtastic

SDS-PAGE and Western Blotting

  • 1,094 topics
  • 3,727 replies
how to choose a proper tag... - last post by Biogareth

Immunology

Discussions on antibody, immunohistochemistry and more...

  • 1,616 topics
  • 2,636 replies
Having issues with tetramer... - last post by Ahrenhase

Microbiology

Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,003 topics
  • 2,929 replies
Protocols to identify or pu... - last post by bob1

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 196 topics
  • 324 replies
Can we identify genetic mar... - last post by Raywalker09

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 600 topics
  • 1,607 replies
MNase assay - how to quanti... - last post by phage434

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 320 topics
  • 952 replies
No signal in qPCR from ChIP... - last post by ndswxy

Macroarray and Microarray

Topics related to different array techniques

  • 120 topics
  • 219 replies
Array Data for tumours (lun... - last post by Ahyi

Neuroscience

Questions related to neuroscience and other neuro-related fields

  • 183 topics
  • 257 replies
Questions on mind uploading - last post by bob1

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 178 topics
  • 406 replies
CpG injection for IFNalpha... - last post by BartL

Botany and Plant Biology

Topics specific to plants.

  • 104 topics
  • 245 replies
problems on the media for o... - last post by tamarine

Pharmacology and Pharmaceutics

  • 75 topics
  • 143 replies
What is the best statisical... - last post by DRT

Histology and Pathology

  • 126 topics
  • 177 replies
Frozen tissue, could it be... - last post by immunovir

Hematology

  • 32 topics
  • 82 replies
how to make Percoll solutio... - last post by mdfenko

Chemistry

  • 57 topics
  • 151 replies
Acid-Base Buffer Question - last post by bob1



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