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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,826 topics
  • 5,868 replies
Acceptable RNA A260/280 ratio - last post by 5280

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 609 topics
  • 1,140 replies
Estimating the allele age - last post by kenta


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 483 topics
  • 1,003 replies
Measuring ammonia in cell l... - last post by TrivediScience

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 132 topics
  • 338 replies
the difference between Site... - last post by kelly1

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,194 topics
  • 3,820 replies
how to confirm lentiviral i... - last post by 5280

Tissue and Cell Culture

  • 1,705 topics
  • 3,847 replies
Invasion assay: analysis qu... - last post by SusieQ

Stem Cell

  • 136 topics
  • 206 replies
Stem Cell Culture Theory - last post by CandyTon

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 408 topics
  • 846 replies
DNA labeling Plasmodium ber... - last post by Jessica Filtjens

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,114 topics
  • 12,898 replies
Samples accidentally thawed - last post by 5280

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,894 topics
  • 6,275 replies
maximal yeast vector size - last post by Bounmi

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,216 topics
  • 6,155 replies
pcr primer design - last post by aldoskys

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 637 topics
  • 1,345 replies
Can I use housekeeper like... - last post by -hiu-

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,052 topics
  • 3,992 replies
Unbroken cell - last post by merlav

Protein Expression and Purification

  • 750 topics
  • 1,838 replies
how to remove affinity tag... - last post by CandyTon

SDS-PAGE and Western Blotting

  • 1,129 topics
  • 3,836 replies
Antibody not binding to low... - last post by mdfenko


Discussions on antibody, immunohistochemistry and more...

  • 1,638 topics
  • 2,685 replies
Can't see my molecular... - last post by mdfenko


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,027 topics
  • 2,991 replies
Anaerobes in CO2 incubators - last post by phage434

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 204 topics
  • 329 replies
Estimating the allele age - last post by kenta

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 604 topics
  • 1,613 replies
Tab-seq or oxBS-seq for loc... - last post by Galleg

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 330 topics
  • 969 replies
Role of RNA Sequencing Prim... - last post by cellculture_newbie

Macroarray and Microarray

Topics related to different array techniques

  • 125 topics
  • 226 replies
online microarray data - last post by Srilatha Dharmavaram


Questions related to neuroscience and other neuro-related fields

  • 188 topics
  • 265 replies
Immunocytochemistry neurons... - last post by bob1

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 181 topics
  • 413 replies
Electrically charged insect... - last post by hobglobin

Botany and Plant Biology

Topics specific to plants.

  • 109 topics
  • 262 replies
Help to sterilize unknown seed - last post by tamarine

Pharmacology and Pharmaceutics

  • 79 topics
  • 146 replies
Upstream Process Developmen... - last post by biomart

Histology and Pathology

  • 139 topics
  • 188 replies
LUNA Staining Protocol to d... - last post by Empath


  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof


  • 57 topics
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5 M NaCl stock - last post by Ahyi

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