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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,945 topics
  • 6,127 replies
How does the RBC lysis buff... - last post by bob1

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 655 topics
  • 1,198 replies
Three experiments with thre... - last post by DRT


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 522 topics
  • 1,108 replies
amount of proteins in urine - last post by Zagami Francesco

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 141 topics
  • 360 replies
How do we know a study is e... - last post by hobglobin

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,243 topics
  • 3,870 replies
crystal violet staining - last post by Zagami Francesco

Tissue and Cell Culture

  • 1,813 topics
  • 3,990 replies
Yeast contamination with fu... - last post by Abhi42

Stem Cell

  • 139 topics
  • 211 replies
Cryopreserving human ES dir... - last post by neuropath

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 427 topics
  • 863 replies
FAC Sorting of live cells b... - last post by neuropath

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,263 topics
  • 13,151 replies
Free software for DNA finge... - last post by hobglobin

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,975 topics
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p1 transduction into recA-... - last post by Raygoza

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,341 topics
  • 6,401 replies
circular RNA - last post by noez_

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 664 topics
  • 1,387 replies
We need a way to see microR... - last post by Fredreload

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,114 topics
  • 4,143 replies
n=3 for pSILAC - last post by bob1

Protein Expression and Purification

  • 819 topics
  • 2,030 replies
How to make antibody based... - last post by mdfenko

SDS-PAGE and Western Blotting

  • 1,197 topics
  • 4,034 replies
Need help with my SDS-PAGE... - last post by Doraid


Discussions on antibody, immunohistochemistry and more...

  • 1,675 topics
  • 2,765 replies
How to polarize TH17 cells... - last post by Chris22


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,101 topics
  • 3,198 replies
problem in preparing minima... - last post by bob1

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 224 topics
  • 346 replies
Pipeline to search bacteria... - last post by Chris22

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 621 topics
  • 1,664 replies
Different efficiencies for... - last post by eyes

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 349 topics
  • 989 replies
Variable ChiP-qPCR even in... - last post by Sandeep Gupta

Macroarray and Microarray

Topics related to different array techniques

  • 129 topics
  • 234 replies
online microarray data - last post by LizRepositive


Questions related to neuroscience and other neuro-related fields

  • 193 topics
  • 267 replies
What is the best dopaminerg... - last post by Katlnz

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 185 topics
  • 419 replies
Is it possible to cause mor... - last post by molbiolnew

Botany and Plant Biology

Topics specific to plants.

  • 117 topics
  • 281 replies
In vitro culture saffron - last post by mdfenko

Pharmacology and Pharmaceutics

  • 84 topics
  • 164 replies
Crosslinking with glutarald... - last post by labtastic

Histology and Pathology

  • 158 topics
  • 204 replies
unusual FITC background sta... - last post by biochem06


  • 33 topics
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Freezing PBMCs that had bee... - last post by Trof


  • 62 topics
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solid phase extraction cort... - last post by mdfenko

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