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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,929 topics
  • 6,105 replies
Difference between HEPES an... - last post by rick112

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 651 topics
  • 1,194 replies
Looking for dataset of prot... - last post by LizRepositive


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 515 topics
  • 1,082 replies
Reversing Formaldehyde cros... - last post by Jen

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 139 topics
  • 357 replies
A contagious man infecting... - last post by bob1

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,239 topics
  • 3,867 replies
Lysis of osteoblastic cell... - last post by ocram

Tissue and Cell Culture

  • 1,800 topics
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Question of Toxin and cell... - last post by Montys

Stem Cell

  • 139 topics
  • 211 replies
Cryopreserving human ES dir... - last post by neuropath

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 422 topics
  • 859 replies
Sample is not running into... - last post by Marco MHC

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,241 topics
  • 13,107 replies
Purification of linearized... - last post by Deba_Bio

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,963 topics
  • 6,482 replies
In-fusion HD cloning - last post by bob1

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,324 topics
  • 6,373 replies
Pre-designed qRT-PCR primers - last post by Trof

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 658 topics
  • 1,371 replies
negative control miRNA - last post by bob1

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,108 topics
  • 4,135 replies
Mass Spectrometry help needed - last post by rick112

Protein Expression and Purification

  • 806 topics
  • 2,000 replies
AKTA FPLC - UV instability - last post by mdfenko

SDS-PAGE and Western Blotting

  • 1,192 topics
  • 4,027 replies
Automated Western blot systems - last post by lisam


Discussions on antibody, immunohistochemistry and more...

  • 1,670 topics
  • 2,756 replies
Anti-CD3 stimulated IL-2 cy... - last post by Mireille


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,096 topics
  • 3,188 replies
Troubleshooting pelleting i... - last post by El Crazy Xabi

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 218 topics
  • 344 replies
Finding promoter regions - last post by Ken S

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 620 topics
  • 1,662 replies
Online tools for methylatio... - last post by LizRepositive

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 347 topics
  • 989 replies
Radiactive PCR for ChIP qua... - last post by autophagator

Macroarray and Microarray

Topics related to different array techniques

  • 129 topics
  • 234 replies
online microarray data - last post by LizRepositive


Questions related to neuroscience and other neuro-related fields

  • 192 topics
  • 267 replies
What is EPSP slope ? - last post by Inmost sun

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 184 topics
  • 418 replies
Free software to analyze IV... - last post by neuropath

Botany and Plant Biology

Topics specific to plants.

  • 116 topics
  • 280 replies
What does "R.H. 1619... - last post by nbic

Pharmacology and Pharmaceutics

  • 83 topics
  • 163 replies
Calculating whole body toxi... - last post by bob1

Histology and Pathology

  • 157 topics
  • 202 replies
IHC Protein Competition, Pr... - last post by bob1


  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof


  • 59 topics
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EDTA precipitates out of ED... - last post by mdfenko

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