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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,997 topics
  • 6,234 replies
Cell counting - last post by bob1

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 667 topics
  • 1,223 replies
Which regression model used... - last post by bob1


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 528 topics
  • 1,127 replies
PBS or TBS, where cannot us... - last post by mdfenko

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 144 topics
  • 362 replies
New generation "vaccine... - last post by henrilentonen

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,257 topics
  • 3,887 replies
Oxidative stress assay and... - last post by moses.jala

Tissue and Cell Culture

  • 1,840 topics
  • 4,036 replies
MNFS60 cells are dying. - last post by bob1

Stem Cell

  • 139 topics
  • 211 replies
Cryopreserving human ES dir... - last post by neuropath

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 435 topics
  • 867 replies
Two distinct populations ob... - last post by OldCloner

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,299 topics
  • 13,201 replies
ATP analogues - last post by itmaybe

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,988 topics
  • 6,544 replies
Troubleshooting with restri... - last post by OldCloner

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,376 topics
  • 6,472 replies
Dengue Virus Serotyping - last post by Faiz Ahmed Raza

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 666 topics
  • 1,388 replies
miRNA isolation from serum - last post by seomelon06

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,134 topics
  • 4,179 replies
blood contamination - last post by Zagami Francesco

Protein Expression and Purification

  • 831 topics
  • 2,057 replies
A single point mutation enh... - last post by mdfenko

SDS-PAGE and Western Blotting

  • 1,214 topics
  • 4,080 replies
why Tris-glycine buffer div... - last post by bob1


Discussions on antibody, immunohistochemistry and more...

  • 1,690 topics
  • 2,778 replies
Help, how to perfuse mouse,... - last post by yl2j


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,130 topics
  • 3,279 replies
Is the unit cfu/swab the sa... - last post by bob1

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 230 topics
  • 352 replies
HPRT mutation & 6-TG se... - last post by lilyasia

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 624 topics
  • 1,667 replies
"Binomial" and its... - last post by MindlessJPEG

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 350 topics
  • 991 replies
Need some troubleshooting a... - last post by Florian_LB

Macroarray and Microarray

Topics related to different array techniques

  • 131 topics
  • 235 replies
Agilent Microarray Data Ana... - last post by kwiggins


Questions related to neuroscience and other neuro-related fields

  • 195 topics
  • 270 replies
Immunofluorescence quantifi... - last post by bob1

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 185 topics
  • 419 replies
Is it possible to cause mor... - last post by molbiolnew

Botany and Plant Biology

Topics specific to plants.

  • 121 topics
  • 283 replies
Chlorophyll and other pigme... - last post by AdOl

Pharmacology and Pharmaceutics

  • 84 topics
  • 164 replies
Crosslinking with glutarald... - last post by labtastic

Histology and Pathology

  • 171 topics
  • 216 replies
Gomori trichrome stain - last post by bob1


  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof


  • 66 topics
  • 171 replies
what solution in upper laye... - last post by bob1

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