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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,876 topics
  • 6,000 replies
Agarose Gel consistency issues - last post by Barcoder

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 626 topics
  • 1,172 replies
Statistical analysis for drug - last post by Harrison


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 495 topics
  • 1,038 replies
RBC lysis bufer please help - last post by mdfenko

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 134 topics
  • 346 replies
Design Format - last post by wswr

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,221 topics
  • 3,848 replies
Migration Inhibitors - last post by moljul

Tissue and Cell Culture

  • 1,756 topics
  • 3,918 replies
immunofluorescence  stainin... - last post by bob1

Stem Cell

  • 138 topics
  • 211 replies
Stem Cell Donation - last post by Chloe Mica

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 416 topics
  • 851 replies
Hoechst33342/7-AAD staining - last post by TinaTeaspoon

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,186 topics
  • 13,034 replies
DNA extraction from FFPE ti... - last post by p.blaze53

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,935 topics
  • 6,395 replies
Lower Plasmid Yields - last post by bob1

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,289 topics
  • 6,309 replies
Using qPCR machine for simp... - last post by bob1

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 652 topics
  • 1,356 replies
Question regarding small nu... - last post by Mohamed 1984

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,077 topics
  • 4,061 replies
Protein denaturation - last post by itmaybe

Protein Expression and Purification

  • 774 topics
  • 1,920 replies
Need to include protease in... - last post by gvbdxz

SDS-PAGE and Western Blotting

  • 1,160 topics
  • 3,952 replies
Bubbles in western blot gel... - last post by mdfenko


Discussions on antibody, immunohistochemistry and more...

  • 1,651 topics
  • 2,711 replies
Combining alpha and beta TC... - last post by Ahrenhase


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,062 topics
  • 3,134 replies
media salinity and ph - last post by El Crazy Xabi

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 213 topics
  • 339 replies
Sex determining from scats ? - last post by phage434

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 615 topics
  • 1,656 replies
bisulfte conversion with Am... - last post by hiri

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 338 topics
  • 974 replies
CHIP-seq problem with yeast... - last post by lorenzo

Macroarray and Microarray

Topics related to different array techniques

  • 128 topics
  • 232 replies
Microarray resources - last post by mdfenko


Questions related to neuroscience and other neuro-related fields

  • 191 topics
  • 265 replies
Why GFP/YFP in neurons is l... - last post by Marinavv

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 182 topics
  • 416 replies
Routine medical tests on mi... - last post by pito

Botany and Plant Biology

Topics specific to plants.

  • 112 topics
  • 270 replies
Agrobacterium transformatio... - last post by Erik Solhaug

Pharmacology and Pharmaceutics

  • 80 topics
  • 151 replies
Calculating Drug concentrat... - last post by murusa

Histology and Pathology

  • 142 topics
  • 192 replies
Many cracks on FFPE block a... - last post by Rnotk


  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof


  • 59 topics
  • 155 replies
Agilent ChemStation (chiral... - last post by mdfenko

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