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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,841 topics
  • 5,915 replies
PCR product not migrating f... - last post by mdfenko

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 612 topics
  • 1,145 replies
Different calculation of Br... - last post by DRT


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 487 topics
  • 1,025 replies
Please HELP with an Biochem... - last post by JDSBlueDevl

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 133 topics
  • 339 replies
comprehensive search for a... - last post by phdgirl

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,206 topics
  • 3,834 replies
control shRNA - last post by Lee6948

Tissue and Cell Culture

  • 1,727 topics
  • 3,877 replies
Cell clumps or contaminatio... - last post by bob1

Stem Cell

  • 137 topics
  • 210 replies
PCR DIG-labeling kit preblem? - last post by bob1

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 410 topics
  • 846 replies
How to clean up cell fragme... - last post by SoggyJaw

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,139 topics
  • 12,935 replies
DNA concentration no spectr... - last post by r.rosati

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,913 topics
  • 6,341 replies
Problem with double digest... - last post by astroboy89

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,247 topics
  • 6,224 replies
Experimental design for RT-PCR - last post by awan

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 641 topics
  • 1,349 replies
off target effects - last post by bongiwoman

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,066 topics
  • 4,028 replies
0.1-0.25% Sodium Deoxychola... - last post by asaren

Protein Expression and Purification

  • 760 topics
  • 1,875 replies
Thrombin cleaved GST-fusion... - last post by ibmbio

SDS-PAGE and Western Blotting

  • 1,143 topics
  • 3,873 replies
detection of small peptide... - last post by marzieh


Discussions on antibody, immunohistochemistry and more...

  • 1,646 topics
  • 2,695 replies
difference in b cell and t... - last post by Hiral Mistry


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,041 topics
  • 3,026 replies
Making bacteria stock using... - last post by r.rosati

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 208 topics
  • 332 replies
Help Finding Genes In Other... - last post by bob1

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 610 topics
  • 1,636 replies
why not working msp primers - last post by zyk

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 333 topics
  • 972 replies
MiSeq Reporter program - last post by moerae

Macroarray and Microarray

Topics related to different array techniques

  • 125 topics
  • 226 replies
online microarray data - last post by Srilatha Dharmavaram


Questions related to neuroscience and other neuro-related fields

  • 190 topics
  • 265 replies
need help with nerves and r... - last post by sirius

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 182 topics
  • 416 replies
Routine medical tests on mi... - last post by pito

Botany and Plant Biology

Topics specific to plants.

  • 111 topics
  • 269 replies
What plant species are you... - last post by tamarine

Pharmacology and Pharmaceutics

  • 80 topics
  • 150 replies
Preferred assay for measuri... - last post by DRT

Histology and Pathology

  • 139 topics
  • 188 replies
LUNA Staining Protocol to d... - last post by Empath


  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof


  • 57 topics
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5 M NaCl stock - last post by Ahyi

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