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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,871 topics
  • 5,995 replies
How to extract Biotinylated... - last post by Rosewater

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 624 topics
  • 1,169 replies
Splitting a multi-model PDB... - last post by bob1


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 493 topics
  • 1,033 replies
using IC50 to determine dos... - last post by sialic acid

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 134 topics
  • 343 replies
Design Format - last post by hobglobin

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,219 topics
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E-Cadherin Expression in OA... - last post by J092

Tissue and Cell Culture

  • 1,744 topics
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SMC turn to Fibroblasts - last post by Delta

Stem Cell

  • 138 topics
  • 211 replies
Stem Cell Donation - last post by Chloe Mica

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 415 topics
  • 850 replies
Best fluorescence spectral... - last post by seanspotatobusiness

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,173 topics
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Current Technology - last post by Phil Pense

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,929 topics
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Phage curing - last post by Raygoza

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,285 topics
  • 6,300 replies
What cycle is best for anal... - last post by Vyse

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 648 topics
  • 1,356 replies
Anyone ever mixed two siRNA... - last post by Sakumi

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,072 topics
  • 4,047 replies
General questions for enzym... - last post by james21

Protein Expression and Purification

  • 771 topics
  • 1,909 replies
Cloned gene in pET system n... - last post by labtastic

SDS-PAGE and Western Blotting

  • 1,157 topics
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Western Sensitivity: ECL ve... - last post by Artemis2007


Discussions on antibody, immunohistochemistry and more...

  • 1,650 topics
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Inactivating effects on imm... - last post by jamieoverton727


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,058 topics
  • 3,126 replies
Advice on test results (mic... - last post by Phil Geis

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 211 topics
  • 336 replies
Tamoxifen inducible Cre to... - last post by Wickerl3732

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 615 topics
  • 1,656 replies
bisulfte conversion with Am... - last post by hiri

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 337 topics
  • 974 replies
Chip Assay-Cell type? - last post by Zohreh_fa

Macroarray and Microarray

Topics related to different array techniques

  • 127 topics
  • 229 replies
RNA extraction/trizol - last post by mdfenko


Questions related to neuroscience and other neuro-related fields

  • 191 topics
  • 265 replies
Why GFP/YFP in neurons is l... - last post by Marinavv

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 182 topics
  • 416 replies
Routine medical tests on mi... - last post by pito

Botany and Plant Biology

Topics specific to plants.

  • 112 topics
  • 270 replies
Agrobacterium transformatio... - last post by Erik Solhaug

Pharmacology and Pharmaceutics

  • 80 topics
  • 150 replies
Preferred assay for measuri... - last post by DRT

Histology and Pathology

  • 141 topics
  • 192 replies
Distinguishing two populati... - last post by cardosopedro


  • 33 topics
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Freezing PBMCs that had bee... - last post by Trof


  • 59 topics
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Agilent ChemStation (chiral... - last post by mdfenko

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