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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,893 topics
  • 6,028 replies
Conversion of milligram wei... - last post by mdfenko

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 641 topics
  • 1,182 replies
Mutation - last post by pito


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 504 topics
  • 1,057 replies
industial scale protein pro... - last post by Chomolungma

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 137 topics
  • 350 replies
Enhancer Identification for... - last post by SarahLee8

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,225 topics
  • 3,856 replies
Synchronization of HEK293T... - last post by bob1

Tissue and Cell Culture

  • 1,776 topics
  • 3,939 replies
Proliferation Inhibitors? - last post by moljul

Stem Cell

  • 138 topics
  • 211 replies
Stem Cell Donation - last post by Chloe Mica

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 418 topics
  • 855 replies
What are the uses for posit... - last post by ink12

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,208 topics
  • 13,072 replies
HTP DNA and RNA extraction... - last post by Ashlay Donlad

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,949 topics
  • 6,432 replies
Problem with possible leaky... - last post by Meg P. Anula

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,301 topics
  • 6,329 replies
PCR contamination - last post by merlav

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 654 topics
  • 1,358 replies
miRNA Identification - last post by Jon Moulton

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,088 topics
  • 4,078 replies
iTRAQ or ELISA? - last post by BioPe

Protein Expression and Purification

  • 785 topics
  • 1,941 replies
Determining Cofactor for In... - last post by bob1

SDS-PAGE and Western Blotting

  • 1,171 topics
  • 3,970 replies
More than 10 min exposure t... - last post by Meg P. Anula


Discussions on antibody, immunohistochemistry and more...

  • 1,654 topics
  • 2,718 replies
Confusing ELISA results - n... - last post by tonix37


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,070 topics
  • 3,147 replies
How homologous do (endogeno... - last post by Chris22

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 214 topics
  • 341 replies
Enhancer Identification for... - last post by SarahLee8

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 617 topics
  • 1,659 replies
inconsistent and low reprod... - last post by MaryC

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 341 topics
  • 980 replies
fragmentation single cells - last post by molbio1234

Macroarray and Microarray

Topics related to different array techniques

  • 128 topics
  • 232 replies
Microarray resources - last post by mdfenko


Questions related to neuroscience and other neuro-related fields

  • 192 topics
  • 265 replies
ELISA dopamine - last post by martin-sanchez

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 182 topics
  • 416 replies
Routine medical tests on mi... - last post by pito

Botany and Plant Biology

Topics specific to plants.

  • 114 topics
  • 270 replies
chlorophyll density in plants - last post by MrZwing

Pharmacology and Pharmaceutics

  • 80 topics
  • 151 replies
Calculating Drug concentrat... - last post by murusa

Histology and Pathology

  • 146 topics
  • 200 replies
Fast Red B salt - last post by smica602


  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof


  • 59 topics
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EDTA precipitates out of ED... - last post by mdfenko

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