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Protocols and Techniques Forums


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General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,813 topics
  • 5,830 replies
SDS page staning issue - last post by mdfenko

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 606 topics
  • 1,140 replies
Power Analyses - last post by Alternative

Biochemistry

Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 480 topics
  • 1,001 replies
RNA extraction and DNase tr... - last post by Kamil

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 128 topics
  • 332 replies
On How to Build a "Knoc... - last post by Curtis

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,188 topics
  • 3,813 replies
Cell strainer for 24 Well P... - last post by profmk

Tissue and Cell Culture

  • 1,693 topics
  • 3,826 replies
cell counting - last post by Keity

Stem Cell

  • 136 topics
  • 204 replies
Problem in mouse embryonic... - last post by dhanur

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 402 topics
  • 846 replies
Biotin-streptavidin signal... - last post by sheldon

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,098 topics
  • 12,865 replies
Canīt get rid of DNA contam... - last post by Katuss

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,884 topics
  • 6,241 replies
When designing a Mammalian... - last post by Smog187

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,209 topics
  • 6,126 replies
Random chuck of nonsense in... - last post by TheFOrsh

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 636 topics
  • 1,341 replies
Can I use housekeeper like... - last post by -hiu-

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,046 topics
  • 3,989 replies
Trouble eluting all protein... - last post by neuron

Protein Expression and Purification

  • 747 topics
  • 1,829 replies
Anion Exchange Buffers - last post by Smog187

SDS-PAGE and Western Blotting

  • 1,125 topics
  • 3,819 replies
Question on loss of signal... - last post by Gradi

Immunology

Discussions on antibody, immunohistochemistry and more...

  • 1,634 topics
  • 2,681 replies
concentration of samples wi... - last post by sgt4boston

Microbiology

Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,024 topics
  • 2,981 replies
unpredictable results in gr... - last post by bob1

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 199 topics
  • 325 replies
Mutation rate - last post by p.blaze53

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 603 topics
  • 1,612 replies
Online tools for methylatio... - last post by rakun

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 328 topics
  • 969 replies
ChIP-seq controls question - last post by ionna

Macroarray and Microarray

Topics related to different array techniques

  • 125 topics
  • 226 replies
online microarray data - last post by Srilatha Dharmavaram

Neuroscience

Questions related to neuroscience and other neuro-related fields

  • 188 topics
  • 265 replies
Immunocytochemistry neurons... - last post by bob1

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 181 topics
  • 413 replies
Electrically charged insect... - last post by hobglobin

Botany and Plant Biology

Topics specific to plants.

  • 109 topics
  • 261 replies
Help to sterilize unknown seed - last post by harrypotter

Pharmacology and Pharmaceutics

  • 78 topics
  • 145 replies
introducing alkyne or azide... - last post by Hehehe

Histology and Pathology

  • 137 topics
  • 185 replies
Help ID fluoescently-labell... - last post by MC_london

Hematology

  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof

Chemistry

  • 57 topics
  • 152 replies
5 M NaCl stock - last post by Ahyi



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