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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,834 topics
  • 5,894 replies
Lack of reproducibility usi... - last post by Michael Starr

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 610 topics
  • 1,143 replies
The depth and coverage of R... - last post by Mohamed 1984


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 485 topics
  • 1,020 replies
Pull-Down Assay, EMSA Assay... - last post by mdfenko

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 133 topics
  • 339 replies
comprehensive search for a... - last post by phdgirl

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,199 topics
  • 3,822 replies
Distribution of cell recept... - last post by camille95r

Tissue and Cell Culture

  • 1,718 topics
  • 3,862 replies
Can foam created when resus... - last post by bob1

Stem Cell

  • 136 topics
  • 207 replies
Problem in mouse embryonic... - last post by KirstenOrion

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 409 topics
  • 846 replies
Which markers to distinguis... - last post by EvelynMDC

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,123 topics
  • 12,911 replies
Isolation of genomic DNA fo... - last post by r.rosati

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,907 topics
  • 6,308 replies
Double digestion problem wi... - last post by phage434

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,228 topics
  • 6,168 replies
Shape curve realtime PCR - last post by cc-diagn

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 639 topics
  • 1,347 replies
cell viability effect and i... - last post by bongiwoman

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,061 topics
  • 4,019 replies
What post-translational mod... - last post by miST32

Protein Expression and Purification

  • 754 topics
  • 1,862 replies
Predicting amount maximum o... - last post by mdfenko

SDS-PAGE and Western Blotting

  • 1,137 topics
  • 3,855 replies
Cell lysis - last post by TheFOrsh


Discussions on antibody, immunohistochemistry and more...

  • 1,643 topics
  • 2,690 replies
Mouse Germline Hybridoma Se... - last post by SD2433


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,035 topics
  • 3,008 replies
need to make a new glycerol... - last post by pito

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 206 topics
  • 331 replies
SNP arrays (23andme and oth... - last post by Trof

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 606 topics
  • 1,615 replies
DBTSS use - last post by rakun

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 330 topics
  • 969 replies
Role of RNA Sequencing Prim... - last post by cellculture_newbie

Macroarray and Microarray

Topics related to different array techniques

  • 125 topics
  • 226 replies
online microarray data - last post by Srilatha Dharmavaram


Questions related to neuroscience and other neuro-related fields

  • 188 topics
  • 265 replies
Immunocytochemistry neurons... - last post by bob1

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 182 topics
  • 416 replies
Routine medical tests on mi... - last post by pito

Botany and Plant Biology

Topics specific to plants.

  • 110 topics
  • 262 replies
tomato transformation - bas... - last post by cahyaprihatna

Pharmacology and Pharmaceutics

  • 80 topics
  • 147 replies
Cell lines behaving differe... - last post by DRT

Histology and Pathology

  • 139 topics
  • 188 replies
LUNA Staining Protocol to d... - last post by Empath


  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof


  • 57 topics
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5 M NaCl stock - last post by Ahyi

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