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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,914 topics
  • 6,056 replies
Dilution help - last post by molbio1234

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 647 topics
  • 1,188 replies
Can someone explain in laym... - last post by bob1


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 508 topics
  • 1,061 replies
non- linear Scatchard plot... - last post by mdfenko

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 138 topics
  • 355 replies
Start a Lab in My Garage -... - last post by El Crazy Xabi

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,232 topics
  • 3,862 replies
Luciferase assay - experime... - last post by bob1

Tissue and Cell Culture

  • 1,786 topics
  • 3,944 replies
Alkaline phosphatase activi... - last post by Zagami Francesco

Stem Cell

  • 138 topics
  • 211 replies
Stem Cell Donation - last post by Chloe Mica

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 419 topics
  • 858 replies
How to clean up cell fragme... - last post by Andrea Fortina

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,219 topics
  • 13,084 replies
GFP transfection - last post by SF_HK

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,959 topics
  • 6,456 replies
Can someone please explain... - last post by Questions

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,315 topics
  • 6,355 replies
reliable homemade mastermix... - last post by lorenzo

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 657 topics
  • 1,369 replies
Mature miRNA in expression... - last post by Andrea Fortina

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,104 topics
  • 4,123 replies
Strange Mass spectrometry r... - last post by biochem06

Protein Expression and Purification

  • 797 topics
  • 1,975 replies
GST fusion protein - two do... - last post by Missle

SDS-PAGE and Western Blotting

  • 1,186 topics
  • 4,006 replies
gelatin zymogram - last post by bob1


Discussions on antibody, immunohistochemistry and more...

  • 1,660 topics
  • 2,729 replies
Good ovalbumin antibody - last post by Therapeutic Antibodies


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,083 topics
  • 3,173 replies
Can I use Duran bottle inst... - last post by atea

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 216 topics
  • 341 replies
How to match the alleles be... - last post by Tszki

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 618 topics
  • 1,661 replies
Baseline Noise on Bisulfite... - last post by MaryC

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 341 topics
  • 984 replies
Sonication woes for Chip-se... - last post by AtomWang

Macroarray and Microarray

Topics related to different array techniques

  • 128 topics
  • 232 replies
Microarray resources - last post by mdfenko


Questions related to neuroscience and other neuro-related fields

  • 192 topics
  • 265 replies
ELISA dopamine - last post by martin-sanchez

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 182 topics
  • 416 replies
Routine medical tests on mi... - last post by pito

Botany and Plant Biology

Topics specific to plants.

  • 115 topics
  • 270 replies
Grow Arabidopsis on rockwool - last post by Nay Vanit

Pharmacology and Pharmaceutics

  • 81 topics
  • 151 replies
Degarelix Benefits to Reduc... - last post by Chloe Mica

Histology and Pathology

  • 153 topics
  • 201 replies
DNA Extraction from Tissue... - last post by merlav


  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof


  • 59 topics
  • 158 replies
EDTA precipitates out of ED... - last post by mdfenko

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