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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 2,032 topics
  • 6,305 replies
Protein concentration for e... - last post by mdfenko

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 685 topics
  • 1,235 replies
How to statistically/bioinf... - last post by bob1


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 532 topics
  • 1,140 replies
Rotating magnetic fields ag... - last post by sciencetoolbar

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 148 topics
  • 366 replies
Design Format - last post by Stephbaker

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,265 topics
  • 3,901 replies
Senescence.... positive res... - last post by bob1

Tissue and Cell Culture

  • 1,845 topics
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what is mean by Mock cell? - last post by pito

Stem Cell

  • 139 topics
  • 212 replies
Differentiation of human co... - last post by Stephbaker

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 436 topics
  • 867 replies
Isotype control vs. stained... - last post by Thomson

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,322 topics
  • 13,230 replies
end point PCR using real ti... - last post by bob1

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,994 topics
  • 6,576 replies
Trouble transforming pET28a... - last post by OldCloner

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,384 topics
  • 6,484 replies
Re-PCR on a gel purified PC... - last post by Curtis

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 671 topics
  • 1,392 replies
Help in gene editing - last post by tesla2020

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,137 topics
  • 4,182 replies
Standard HCL protein hydrol... - last post by mdfenko

Protein Expression and Purification

  • 833 topics
  • 2,060 replies
Virus vs protein purification - last post by mdfenko

SDS-PAGE and Western Blotting

  • 1,218 topics
  • 4,093 replies
Can't remove Ponceau st... - last post by bob1


Discussions on antibody, immunohistochemistry and more...

  • 1,695 topics
  • 2,782 replies
ELISA a biosensor? - last post by bob1


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,137 topics
  • 3,300 replies
Growth Curves for Yeasts an... - last post by pito

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 231 topics
  • 350 replies
Eukariotic Linear Motifs - last post by Ziv

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 624 topics
  • 1,667 replies
"Binomial" and its... - last post by MindlessJPEG

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 352 topics
  • 991 replies
Sample storage during ChIP... - last post by Artemis2007

Macroarray and Microarray

Topics related to different array techniques

  • 133 topics
  • 237 replies
autoantibody array - last post by SF_HK


Questions related to neuroscience and other neuro-related fields

  • 196 topics
  • 272 replies
Immunofluorescence quantifi... - last post by Inmost sun

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 186 topics
  • 419 replies
Locating the Mouse AV Node - last post by Gray.MA

Botany and Plant Biology

Topics specific to plants.

  • 122 topics
  • 283 replies
Germination test - last post by bioxesus

Pharmacology and Pharmaceutics

  • 86 topics
  • 165 replies
Calcium Imaging Calibration - last post by CristianaCris

Histology and Pathology

  • 173 topics
  • 217 replies
Antigen Retrieval - last post by SAT142


  • 33 topics
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Freezing PBMCs that had bee... - last post by Trof


  • 67 topics
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Managing Gibbs free energy - last post by bob1

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