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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,739 topics
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Sonication of small PCR amp... - last post by ForEpi

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 582 topics
  • 1,103 replies
How to run genscan on a lar... - last post by Garima


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 461 topics
  • 964 replies
Enzyme Mechanism Problems - last post by sysbio10

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 116 topics
  • 314 replies
Would someone help me discu... - last post by bob1

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,158 topics
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methods for selective in vi... - last post by Retinoblastoma

Tissue and Cell Culture

  • 1,608 topics
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Splitting Cells that are sh... - last post by procrasemit

Stem Cell

  • 134 topics
  • 197 replies
how to transport stem cells... - last post by RLAD

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 385 topics
  • 802 replies
Viability dye 7-AAD - last post by Shoooda

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 5,991 topics
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page electrophoresis - last post by vageesh pandith

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,799 topics
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primer design with restrict... - last post by phage434

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,129 topics
  • 5,937 replies
Interpreting primer BLAST s... - last post by bjk1985

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 619 topics
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prediction of microRNA targ... - last post by pcrman

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,005 topics
  • 3,896 replies
problems with 1D SDS PAGE - last post by biochem06

Protein Expression and Purification

  • 715 topics
  • 1,780 replies
Missing expression of prote... - last post by shuan

SDS-PAGE and Western Blotting

  • 1,076 topics
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SDS PAGE sample preparation... - last post by kyri_osity


Discussions on antibody, immunohistochemistry and more...

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Problems in direct ELISA to... - last post by Bioscientist


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 979 topics
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Genetic mapping in Escheric... - last post by pDNA

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 189 topics
  • 316 replies
need some advise about PCR... - last post by Ameya P

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 592 topics
  • 1,582 replies
puzzled with PCR outcome af... - last post by Illinu

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 314 topics
  • 944 replies
Dual cross-linking thoughts - last post by guru

Macroarray and Microarray

Topics related to different array techniques

  • 120 topics
  • 219 replies
Array Data for tumours (lun... - last post by Ahyi


Questions related to neuroscience and other neuro-related fields

  • 181 topics
  • 255 replies
post-mortem BBB permeabilit... - last post by CovanceABP

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 175 topics
  • 406 replies
orthotopic mouse model of U... - last post by Fungus_Dreams

Botany and Plant Biology

Topics specific to plants.

  • 102 topics
  • 242 replies
Why would you use DNase as... - last post by sara_molecular

Pharmacology and Pharmaceutics

  • 75 topics
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What is the best statisical... - last post by DRT

Histology and Pathology

  • 122 topics
  • 174 replies
Virtual Slide Software - last post by EAG


  • 31 topics
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Obtaining Serum from frozen... - last post by mdfenko


  • 55 topics
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Does autoclaving salts of m... - last post by p.phadtare

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