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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,939 topics
  • 6,117 replies
Calculation of mg/ml protei... - last post by bob1

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 655 topics
  • 1,199 replies
A basic biostatistic discus... - last post by Qutaibha


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 520 topics
  • 1,097 replies
Restriction Digest with pGE... - last post by bob1

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 139 topics
  • 357 replies
A contagious man infecting... - last post by bob1

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,240 topics
  • 3,867 replies
Freezing cell lysate for la... - last post by matheusmlot

Tissue and Cell Culture

  • 1,806 topics
  • 3,978 replies
żmust treatment be aplied 2... - last post by bob1

Stem Cell

  • 139 topics
  • 211 replies
Cryopreserving human ES dir... - last post by neuropath

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 425 topics
  • 860 replies
Question about FACS of maiz... - last post by jerrywu1987

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,252 topics
  • 13,128 replies
Very low yields for >10... - last post by bob1

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,968 topics
  • 6,491 replies
How large need homology arm... - last post by bob1

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,333 topics
  • 6,391 replies
Length of amplicon for RT r... - last post by Nephrite

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 660 topics
  • 1,372 replies
lizard microRNA and human l... - last post by Fredreload

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,110 topics
  • 4,136 replies
Video contest on stories wi... - last post by CreativeScience

Protein Expression and Purification

  • 809 topics
  • 2,005 replies
Anti-Penta His antibody det... - last post by Mafer

SDS-PAGE and Western Blotting

  • 1,192 topics
  • 4,027 replies
Automated Western blot systems - last post by lisam


Discussions on antibody, immunohistochemistry and more...

  • 1,673 topics
  • 2,762 replies
IF Two Antigen Retrival Steps - last post by mdfenko


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,099 topics
  • 3,191 replies
Making  Active culture and... - last post by Ahmad Ud Din

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 222 topics
  • 346 replies
tRNA folding tool - last post by merlav

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 621 topics
  • 1,664 replies
Different efficiencies for... - last post by eyes

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 347 topics
  • 989 replies
Radiactive PCR for ChIP qua... - last post by autophagator

Macroarray and Microarray

Topics related to different array techniques

  • 129 topics
  • 234 replies
online microarray data - last post by LizRepositive


Questions related to neuroscience and other neuro-related fields

  • 192 topics
  • 267 replies
What is EPSP slope ? - last post by Inmost sun

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 184 topics
  • 418 replies
Free software to analyze IV... - last post by neuropath

Botany and Plant Biology

Topics specific to plants.

  • 116 topics
  • 280 replies
What does "R.H. 1619... - last post by nbic

Pharmacology and Pharmaceutics

  • 83 topics
  • 163 replies
Calculating whole body toxi... - last post by bob1

Histology and Pathology

  • 158 topics
  • 204 replies
unusual FITC background sta... - last post by biochem06


  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof


  • 62 topics
  • 160 replies
solid phase extraction cort... - last post by mdfenko

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