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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,894 topics
  • 6,029 replies
What are the different meth... - last post by Mohamad

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 643 topics
  • 1,182 replies
Densitometry: how to determ... - last post by seanspotatobusiness


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 504 topics
  • 1,057 replies
industial scale protein pro... - last post by Chomolungma

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 137 topics
  • 351 replies
Enhancer Identification for... - last post by Michael Starr

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,227 topics
  • 3,857 replies
Lentivirus packaging (no fl... - last post by miST32

Tissue and Cell Culture

  • 1,778 topics
  • 3,939 replies
Prolonged EGF induction for... - last post by Scienceguy 1

Stem Cell

  • 138 topics
  • 211 replies
Stem Cell Donation - last post by Chloe Mica

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 418 topics
  • 855 replies
What are the uses for posit... - last post by ink12

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,209 topics
  • 13,074 replies
Are relaxed plasmids origin... - last post by phage434

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,950 topics
  • 6,432 replies
Cloning issue with bacteria... - last post by Kyle Strickland

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,301 topics
  • 6,329 replies
PCR contamination - last post by merlav

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 654 topics
  • 1,360 replies
miRNA Identification - last post by Jon Moulton

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,090 topics
  • 4,090 replies
Affinity purification glyci... - last post by mdfenko

Protein Expression and Purification

  • 785 topics
  • 1,941 replies
Determining Cofactor for In... - last post by bob1

SDS-PAGE and Western Blotting

  • 1,172 topics
  • 3,973 replies
SDS-PAGE vertical lane stre... - last post by GNANA


Discussions on antibody, immunohistochemistry and more...

  • 1,655 topics
  • 2,719 replies
I can't believe peptide... - last post by bob1


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,073 topics
  • 3,149 replies
Unit meaning?: vg/mL - last post by bob1

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 214 topics
  • 341 replies
Enhancer Identification for... - last post by SarahLee8

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 617 topics
  • 1,659 replies
inconsistent and low reprod... - last post by MaryC

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 341 topics
  • 980 replies
fragmentation single cells - last post by molbio1234

Macroarray and Microarray

Topics related to different array techniques

  • 128 topics
  • 232 replies
Microarray resources - last post by mdfenko


Questions related to neuroscience and other neuro-related fields

  • 192 topics
  • 265 replies
ELISA dopamine - last post by martin-sanchez

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 182 topics
  • 416 replies
Routine medical tests on mi... - last post by pito

Botany and Plant Biology

Topics specific to plants.

  • 114 topics
  • 270 replies
chlorophyll density in plants - last post by MrZwing

Pharmacology and Pharmaceutics

  • 80 topics
  • 151 replies
Calculating Drug concentrat... - last post by murusa

Histology and Pathology

  • 146 topics
  • 200 replies
Fast Red B salt - last post by smica602


  • 33 topics
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Freezing PBMCs that had bee... - last post by Trof


  • 59 topics
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EDTA precipitates out of ED... - last post by mdfenko

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