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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,921 topics
  • 6,088 replies
Protein detection and quant... - last post by mdfenko

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 647 topics
  • 1,188 replies
Can someone explain in laym... - last post by bob1


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 513 topics
  • 1,065 replies
Sanofi Pasteur is hiring a... - last post by jannyfirman

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 139 topics
  • 357 replies
A contagious man infecting... - last post by bob1

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,236 topics
  • 3,866 replies
MTT assay for suspension ce... - last post by Prersha

Tissue and Cell Culture

  • 1,794 topics
  • 3,960 replies
gene expression in cultured... - last post by bob1

Stem Cell

  • 138 topics
  • 211 replies
Stem Cell Donation - last post by Chloe Mica

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 419 topics
  • 858 replies
How to clean up cell fragme... - last post by Andrea Fortina

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,230 topics
  • 13,093 replies
a half less of plasmid copy... - last post by 827753

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,962 topics
  • 6,480 replies
Plasmid purification for Gi... - last post by 827753

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,318 topics
  • 6,366 replies
making your own qPCR kit! - last post by bob1

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 658 topics
  • 1,371 replies
negative control miRNA - last post by bob1

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,105 topics
  • 4,123 replies
Mass Spectrometry help needed - last post by biochem06

Protein Expression and Purification

  • 801 topics
  • 1,992 replies
Tagged recombinant protein... - last post by mdfenko

SDS-PAGE and Western Blotting

  • 1,191 topics
  • 4,027 replies
Biorad Miniprotean review - last post by bob1


Discussions on antibody, immunohistochemistry and more...

  • 1,668 topics
  • 2,748 replies
Serum Interference in Micro... - last post by Taras


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,090 topics
  • 3,182 replies
Disinfectant Efficacy Testing - last post by Phil Geis

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 217 topics
  • 342 replies
Map Viewer for several genes - last post by Trof

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 619 topics
  • 1,661 replies
MTHFR 1298 legit? does Dr.... - last post by vaarmen

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 346 topics
  • 989 replies
How to identify the genomic... - last post by Sandeep Gupta

Macroarray and Microarray

Topics related to different array techniques

  • 129 topics
  • 233 replies
pesticides - last post by 827753


Questions related to neuroscience and other neuro-related fields

  • 192 topics
  • 267 replies
What is EPSP slope ? - last post by Inmost sun

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 183 topics
  • 416 replies
E13.5 mouse embryo staining... - last post by Ambinlab

Botany and Plant Biology

Topics specific to plants.

  • 116 topics
  • 280 replies
What does "R.H. 1619... - last post by nbic

Pharmacology and Pharmaceutics

  • 82 topics
  • 156 replies
Preparation of antibiotic disc - last post by mdfenko

Histology and Pathology

  • 155 topics
  • 201 replies
Stability of Fluoro Jade B... - last post by -hiu-


  • 33 topics
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Freezing PBMCs that had bee... - last post by Trof


  • 59 topics
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EDTA precipitates out of ED... - last post by mdfenko

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