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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,785 topics
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cytokine reconstitution wit... - last post by H. Aly

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 595 topics
  • 1,125 replies
Case-control study using SP... - last post by sayk


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 473 topics
  • 987 replies
Non-radioactive DNA-PK acti... - last post by mdfenko

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 120 topics
  • 317 replies
Problem with gel contractio... - last post by SiaoMin

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,171 topics
  • 3,771 replies
Does Opti-mem contain serum - last post by VarianWrynn

Tissue and Cell Culture

  • 1,662 topics
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Best cryovials for liquid n... - last post by Artemis2007

Stem Cell

  • 135 topics
  • 201 replies
Stem Cell Culture Theory - last post by bob1

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 391 topics
  • 819 replies
TO-PRO3 HELP!! - last post by Giusta

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,049 topics
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Digestion necessary after PCR? - last post by phage434

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,845 topics
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Subtle change in insert siz... - last post by Hamaguchi

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,171 topics
  • 6,039 replies
how to calculate amount of... - last post by molbio1234

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 625 topics
  • 1,324 replies
about microrna overexpressing - last post by adamos1945

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,034 topics
  • 3,959 replies
Band did not migrate on gel... - last post by mdfenko

Protein Expression and Purification

  • 730 topics
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Transient transfection usin... - last post by Olivia Zabel

SDS-PAGE and Western Blotting

  • 1,104 topics
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Glycine vs. tricine - last post by mdfenko


Discussions on antibody, immunohistochemistry and more...

  • 1,618 topics
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How to calculate LOD of elisa - last post by profenpaul


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,009 topics
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Cell counters - Beckman Cou... - last post by Fstevens

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 197 topics
  • 324 replies
Genome annotation - last post by Dav

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 601 topics
  • 1,607 replies
5 aza- 2 deoxy and TSA - last post by seeking_2012

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 322 topics
  • 952 replies
Kapa library quant kit - last post by molbio1234

Macroarray and Microarray

Topics related to different array techniques

  • 121 topics
  • 219 replies
Possibility of comparing di... - last post by tnocs


Questions related to neuroscience and other neuro-related fields

  • 184 topics
  • 258 replies
mouse stellate/cervicothora... - last post by k1064192

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 179 topics
  • 411 replies
female BALB/c mice eating a... - last post by VarianWrynn

Botany and Plant Biology

Topics specific to plants.

  • 106 topics
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Is it possible to stored en... - last post by hobglobin

Pharmacology and Pharmaceutics

  • 77 topics
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Ebola Virus - last post by Einfolge

Histology and Pathology

  • 129 topics
  • 177 replies
Protocole Immunohistochemis... - last post by orianedl


  • 32 topics
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how to make Percoll solutio... - last post by mdfenko


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5 M NaCl stock - last post by Ahyi

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