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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 2,008 topics
  • 6,267 replies
Dilution buffer - last post by bob1

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 672 topics
  • 1,224 replies
question about the sample size - last post by bob1


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 531 topics
  • 1,134 replies
Find documents about probab... - last post by Thi Le

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 148 topics
  • 365 replies
Which healthcare companies... - last post by electros

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,259 topics
  • 3,893 replies
Can glucose-6-phosphate ent... - last post by electros

Tissue and Cell Culture

  • 1,843 topics
  • 4,039 replies
What is going on with these... - last post by bob1

Stem Cell

  • 139 topics
  • 211 replies
Cryopreserving human ES dir... - last post by neuropath

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 436 topics
  • 867 replies
Isotype control vs. stained... - last post by Thomson

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,303 topics
  • 13,204 replies
quantity IHC images - last post by bob1

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,989 topics
  • 6,545 replies
Insert in frame - last post by bob1

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,378 topics
  • 6,474 replies
How do determine the refere... - last post by bob1

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 666 topics
  • 1,388 replies
miRNA isolation from serum - last post by seomelon06

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,134 topics
  • 4,179 replies
blood contamination - last post by Zagami Francesco

Protein Expression and Purification

  • 831 topics
  • 2,058 replies
A single point mutation enh... - last post by rkay447

SDS-PAGE and Western Blotting

  • 1,215 topics
  • 4,082 replies
SDS PAGE bands - last post by LinaAg


Discussions on antibody, immunohistochemistry and more...

  • 1,691 topics
  • 2,778 replies
Immunologic molecules and c... - last post by electros


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,131 topics
  • 3,283 replies
enumeration of anaerobes - last post by sarcidia

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 230 topics
  • 350 replies
molecular biology - last post by mdfenko

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 624 topics
  • 1,667 replies
"Binomial" and its... - last post by MindlessJPEG

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 350 topics
  • 991 replies
Need some troubleshooting a... - last post by Florian_LB

Macroarray and Microarray

Topics related to different array techniques

  • 131 topics
  • 235 replies
Agilent Microarray Data Ana... - last post by kwiggins


Questions related to neuroscience and other neuro-related fields

  • 196 topics
  • 270 replies
What stimuli trigger hippoc... - last post by electros

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 186 topics
  • 419 replies
Locating the Mouse AV Node - last post by Gray.MA

Botany and Plant Biology

Topics specific to plants.

  • 121 topics
  • 283 replies
Chlorophyll and other pigme... - last post by AdOl

Pharmacology and Pharmaceutics

  • 86 topics
  • 165 replies
Calcium Imaging Calibration - last post by CristianaCris

Histology and Pathology

  • 172 topics
  • 216 replies
any suggestions for a good... - last post by hillaryblack


  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof


  • 67 topics
  • 172 replies
Managing Gibbs free energy - last post by bob1

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