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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,868 topics
  • 5,985 replies
How to extract Biotinylated... - last post by Sanjay Premi

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 622 topics
  • 1,162 replies
Docking of a protein with g... - last post by Mariam


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 493 topics
  • 1,032 replies
using IC50 to determine dos... - last post by mdfenko

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 134 topics
  • 343 replies
Design Format - last post by hobglobin

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,216 topics
  • 3,844 replies
Cell death determination - last post by Curtis

Tissue and Cell Culture

  • 1,742 topics
  • 3,904 replies
Secondary goat antirabbit a... - last post by jaya2020

Stem Cell

  • 138 topics
  • 210 replies
Trouble with definitive end... - last post by Delta

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 414 topics
  • 850 replies
Why GFP/YFP+ neurons couldn... - last post by bob1

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,166 topics
  • 13,014 replies
Akt phosphorylation at Thr3... - last post by cancerous

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,927 topics
  • 6,383 replies
Question in gateway system,... - last post by fonie

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,281 topics
  • 6,288 replies
Ligation-mediated PCR: Vent... - last post by Sanjay Premi

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 646 topics
  • 1,354 replies
How to clone two mir-30 bas... - last post by 5280

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,070 topics
  • 4,043 replies
Transfer buffer for tris tr... - last post by bob1

Protein Expression and Purification

  • 768 topics
  • 1,890 replies
His-tag protein not binding... - last post by phage434

SDS-PAGE and Western Blotting

  • 1,155 topics
  • 3,941 replies
Heat Denaturation without a... - last post by mdfenko


Discussions on antibody, immunohistochemistry and more...

  • 1,650 topics
  • 2,710 replies
Avoid drying out of coversl... - last post by mdfenko


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,055 topics
  • 3,090 replies
Addition of 2% glycine in M... - last post by phage434

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 210 topics
  • 336 replies
genotyping choices by scale - last post by angel0071987

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 615 topics
  • 1,655 replies
Of the nature of DNA - last post by Phaeous

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 337 topics
  • 974 replies
Chip Assay-Cell type? - last post by Zohreh_fa

Macroarray and Microarray

Topics related to different array techniques

  • 126 topics
  • 228 replies
Using Gene Ontology - last post by gvbdxz


Questions related to neuroscience and other neuro-related fields

  • 191 topics
  • 265 replies
Why GFP/YFP in neurons is l... - last post by Marinavv

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 182 topics
  • 416 replies
Routine medical tests on mi... - last post by pito

Botany and Plant Biology

Topics specific to plants.

  • 111 topics
  • 270 replies
Microalgae count problem - last post by radioactivestar88

Pharmacology and Pharmaceutics

  • 80 topics
  • 150 replies
Preferred assay for measuri... - last post by DRT

Histology and Pathology

  • 141 topics
  • 192 replies
Distinguishing two populati... - last post by cardosopedro


  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof


  • 59 topics
  • 155 replies
Agilent ChemStation (chiral... - last post by mdfenko

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