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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,688 topics
  • 5,502 replies
Ethidium bromide spill conc... - last post by nymets2013ws

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 568 topics
  • 1,067 replies
Recommend a good textbook f... - last post by mtrnbh


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 450 topics
  • 927 replies
hydroxy-fatty acids and gas... - last post by mdfenko

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 114 topics
  • 300 replies
HPLC detection of 5-methylc... - last post by phage434

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,140 topics
  • 3,730 replies
HRP antibody choice - last post by ulujm

Tissue and Cell Culture

  • 1,563 topics
  • 3,551 replies
easiest cell lines to use - last post by Tabaluga

Stem Cell

  • 130 topics
  • 193 replies
mouse embryonic stem cell c... - last post by Elisa90

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 378 topics
  • 788 replies
Mean of fluorescence in FlowJo - last post by Judith_NM

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 5,926 topics
  • 12,374 replies
Is it possible quantify dsD... - last post by phage434

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,757 topics
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Antibody against H1N1 canno... - last post by bob1

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,081 topics
  • 5,824 replies
Is that normal the negative... - last post by Tabaluga

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 608 topics
  • 1,309 replies
non-radioactive detection o... - last post by Trys

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 1,985 topics
  • 3,860 replies
identifying s-glutathionyla... - last post by PhDinAcronyms

Protein Expression and Purification

  • 697 topics
  • 1,737 replies
regarding protein purificat... - last post by atulp

SDS-PAGE and Western Blotting

  • 1,060 topics
  • 3,585 replies
IgG heavy chain from IP - last post by bob1


Discussions on antibody, immunohistochemistry and more...

  • 1,587 topics
  • 2,592 replies
How to check standard curve... - last post by Leelab


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 938 topics
  • 2,678 replies
NGS of bi-partite viral genome - last post by Curtis

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 183 topics
  • 306 replies
FISH - Cot-1 DNA - last post by biofingers

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 584 topics
  • 1,542 replies
Nested BSP Primer Design - last post by phage434

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 303 topics
  • 934 replies
How to analyze ChIP data fo... - last post by suncokret

Macroarray and Microarray

Topics related to different array techniques

  • 114 topics
  • 214 replies
Ionizing radiation dose for... - last post by sen111


Questions related to neuroscience and other neuro-related fields

  • 178 topics
  • 250 replies
Membrane sensitive dyes: Th... - last post by Wurmbox

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 173 topics
  • 401 replies
Mammary fat pad - last post by smilealie

Botany and Plant Biology

Topics specific to plants.

  • 99 topics
  • 230 replies
What biochemicals are invol... - last post by mogokll

Pharmacology and Pharmaceutics

  • 75 topics
  • 143 replies
What is the best statisical... - last post by DRT

Histology and Pathology

  • 114 topics
  • 157 replies
Questions about Paraffin Se... - last post by gsb218


  • 29 topics
  • 63 replies
Screwed up blood extraction... - last post by Relaxosome


  • 55 topics
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Does autoclaving salts of m... - last post by p.phadtare

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