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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,904 topics
  • 6,048 replies
UV-Imaging Chambers: Oversa... - last post by phage434

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 645 topics
  • 1,187 replies
Laser failure problems with... - last post by jrrosenberg


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 506 topics
  • 1,059 replies
Why would enzyme rate of re... - last post by mdfenko

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 138 topics
  • 355 replies
Start a Lab in My Garage -... - last post by El Crazy Xabi

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,229 topics
  • 3,859 replies
exosome isolation - last post by Michal Levy

Tissue and Cell Culture

  • 1,784 topics
  • 3,943 replies
Help with HeLa cell culture! - last post by matheusmlot

Stem Cell

  • 138 topics
  • 211 replies
Stem Cell Donation - last post by Chloe Mica

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 419 topics
  • 856 replies
Counting beads binding to RBCs - last post by biology_06er

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,215 topics
  • 13,084 replies
RNA mutagenesis - last post by Raygoza

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,956 topics
  • 6,456 replies
Wrong or No fragment in Dir... - last post by aodonnell311

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,311 topics
  • 6,349 replies
testing primers efficiency... - last post by madelingirly

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 656 topics
  • 1,365 replies
miRNA isolation from serum - last post by Moemedawar

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,102 topics
  • 4,119 replies
Effect on protein of low pH? - last post by mdfenko

Protein Expression and Purification

  • 796 topics
  • 1,971 replies
Nonspecific proteins elutin... - last post by Ahrenhase

SDS-PAGE and Western Blotting

  • 1,184 topics
  • 4,004 replies
No or Super Strong Signal - last post by mdfenko


Discussions on antibody, immunohistochemistry and more...

  • 1,659 topics
  • 2,725 replies
Why my bacteria cells look... - last post by mdfenko


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,082 topics
  • 3,167 replies
Formula to calculate bacter... - last post by Meg P. Anula

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 216 topics
  • 341 replies
How to match the alleles be... - last post by Tszki

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 618 topics
  • 1,661 replies
Baseline Noise on Bisulfite... - last post by MaryC

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 341 topics
  • 984 replies
Sonication woes for Chip-se... - last post by AtomWang

Macroarray and Microarray

Topics related to different array techniques

  • 128 topics
  • 232 replies
Microarray resources - last post by mdfenko


Questions related to neuroscience and other neuro-related fields

  • 192 topics
  • 265 replies
ELISA dopamine - last post by martin-sanchez

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 182 topics
  • 416 replies
Routine medical tests on mi... - last post by pito

Botany and Plant Biology

Topics specific to plants.

  • 115 topics
  • 270 replies
Grow Arabidopsis on rockwool - last post by Nay Vanit

Pharmacology and Pharmaceutics

  • 80 topics
  • 151 replies
Calculating Drug concentrat... - last post by murusa

Histology and Pathology

  • 153 topics
  • 201 replies
DNA Extraction from Tissue... - last post by merlav


  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof


  • 59 topics
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EDTA precipitates out of ED... - last post by mdfenko

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