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Protocols and Techniques Forums


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General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,916 topics
  • 6,074 replies
Total recovery of lyophiliz... - last post by bob1

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 647 topics
  • 1,188 replies
Can someone explain in laym... - last post by bob1

Biochemistry

Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 508 topics
  • 1,063 replies
Expression of amino acid co... - last post by Andrea Fortina

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 139 topics
  • 357 replies
A contagious man infecting... - last post by bob1

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,232 topics
  • 3,862 replies
Luciferase assay - experime... - last post by bob1

Tissue and Cell Culture

  • 1,787 topics
  • 3,944 replies
cell viability assay for ca... - last post by prollic

Stem Cell

  • 138 topics
  • 211 replies
Stem Cell Donation - last post by Chloe Mica

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 419 topics
  • 858 replies
How to clean up cell fragme... - last post by Andrea Fortina

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,224 topics
  • 13,086 replies
site directed mutagenesis - last post by SF_HK

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,960 topics
  • 6,475 replies
Migration of circular DNA v... - last post by labtastic

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,316 topics
  • 6,364 replies
Strange Taqman probe negati... - last post by Trof

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 657 topics
  • 1,369 replies
Mature miRNA in expression... - last post by Andrea Fortina

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,105 topics
  • 4,123 replies
Mass Spectrometry help needed - last post by biochem06

Protein Expression and Purification

  • 798 topics
  • 1,983 replies
Use of urea in solubilizing... - last post by mdfenko

SDS-PAGE and Western Blotting

  • 1,188 topics
  • 4,019 replies
patchy/holey bands after tr... - last post by mdfenko

Immunology

Discussions on antibody, immunohistochemistry and more...

  • 1,661 topics
  • 2,739 replies
Where do you purchase T cel... - last post by mdfenko

Microbiology

Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,085 topics
  • 3,173 replies
Can someone please help me... - last post by Celz

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 217 topics
  • 342 replies
Map Viewer for several genes - last post by Trof

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 618 topics
  • 1,661 replies
Baseline Noise on Bisulfite... - last post by MaryC

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 345 topics
  • 988 replies
Bioanalyzer for ChIPed DNA-... - last post by Zohreh_fa

Macroarray and Microarray

Topics related to different array techniques

  • 128 topics
  • 232 replies
Microarray resources - last post by mdfenko

Neuroscience

Questions related to neuroscience and other neuro-related fields

  • 192 topics
  • 265 replies
ELISA dopamine - last post by martin-sanchez

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 182 topics
  • 416 replies
Routine medical tests on mi... - last post by pito

Botany and Plant Biology

Topics specific to plants.

  • 116 topics
  • 280 replies
What does "R.H. 1619... - last post by nbic

Pharmacology and Pharmaceutics

  • 81 topics
  • 151 replies
Degarelix Benefits to Reduc... - last post by Chloe Mica

Histology and Pathology

  • 154 topics
  • 201 replies
LR white resin and H&E... - last post by snd

Hematology

  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof

Chemistry

  • 59 topics
  • 158 replies
EDTA precipitates out of ED... - last post by mdfenko



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