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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,943 topics
  • 6,123 replies
Why do we use 0.5M acetic a... - last post by ShanaShah

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 655 topics
  • 1,198 replies
Three experiments with thre... - last post by DRT


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 522 topics
  • 1,108 replies
amount of proteins in urine - last post by Zagami Francesco

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 139 topics
  • 357 replies
A contagious man infecting... - last post by bob1

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,242 topics
  • 3,867 replies
What is the best dopaminerg... - last post by Katlnz

Tissue and Cell Culture

  • 1,810 topics
  • 3,985 replies
Lentivirus production issues - last post by merlav

Stem Cell

  • 139 topics
  • 211 replies
Cryopreserving human ES dir... - last post by neuropath

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 426 topics
  • 863 replies
What are the uses for posit... - last post by bob1

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,260 topics
  • 13,142 replies
What is your favorite metho... - last post by labtastic

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,972 topics
  • 6,496 replies
Help with making a Dimer - last post by kenny17

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,336 topics
  • 6,393 replies
Different efficiency in GOI... - last post by Paja

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 664 topics
  • 1,380 replies
We need a way to see microR... - last post by Fredreload

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,111 topics
  • 4,140 replies
large scale protein purific... - last post by paramyosin

Protein Expression and Purification

  • 817 topics
  • 2,019 replies
GST fusion protein - two do... - last post by labtastic

SDS-PAGE and Western Blotting

  • 1,195 topics
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Couldn't detect Vimenti... - last post by dvimal27


Discussions on antibody, immunohistochemistry and more...

  • 1,674 topics
  • 2,765 replies
how to separate Antibody an... - last post by Zagami Francesco


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,099 topics
  • 3,192 replies
animal model for shigella - last post by Zagami Francesco

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 224 topics
  • 346 replies
Pipeline to search bacteria... - last post by Chris22

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 621 topics
  • 1,664 replies
Different efficiencies for... - last post by eyes

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 348 topics
  • 989 replies
how to do % input calculati... - last post by Sandeep Gupta

Macroarray and Microarray

Topics related to different array techniques

  • 129 topics
  • 234 replies
online microarray data - last post by LizRepositive


Questions related to neuroscience and other neuro-related fields

  • 193 topics
  • 267 replies
What is the best dopaminerg... - last post by Katlnz

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 184 topics
  • 419 replies
Urine Electrophoresis - last post by Zagami Francesco

Botany and Plant Biology

Topics specific to plants.

  • 116 topics
  • 280 replies
What does "R.H. 1619... - last post by nbic

Pharmacology and Pharmaceutics

  • 83 topics
  • 163 replies
Calculating whole body toxi... - last post by bob1

Histology and Pathology

  • 158 topics
  • 204 replies
unusual FITC background sta... - last post by biochem06


  • 33 topics
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Freezing PBMCs that had bee... - last post by Trof


  • 62 topics
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solid phase extraction cort... - last post by mdfenko

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