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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,984 topics
  • 6,197 replies
Dilution of antibody - last post by bob1

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 660 topics
  • 1,208 replies
News: SIG Workshop to discu... - last post by Arie Helderman


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 526 topics
  • 1,125 replies
Salt in medium - last post by Zagami Francesco

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 143 topics
  • 362 replies
Yre project - last post by Radu Coman

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,252 topics
  • 3,879 replies
Normalise cell count for pr... - last post by bob1

Tissue and Cell Culture

  • 1,829 topics
  • 4,019 replies
293T cells not able to atta... - last post by bob1

Stem Cell

  • 139 topics
  • 211 replies
Cryopreserving human ES dir... - last post by neuropath

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 433 topics
  • 864 replies
Multi-staining 7-aad, Annex... - last post by single_cell_nerd_88

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,290 topics
  • 13,197 replies
ScyTek - ESTABLISHING HUMAN... - last post by scyteklab

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,984 topics
  • 6,526 replies
Determining optimal digesti... - last post by OldCloner

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,364 topics
  • 6,456 replies
what may have gone wrong? - last post by merlav

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 666 topics
  • 1,387 replies
Watch miRNA transcription s... - last post by Jon Moulton

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,134 topics
  • 4,178 replies
Problem with protein induction - last post by sasa_biotech

Protein Expression and Purification

  • 827 topics
  • 2,048 replies
Protein expression issue - last post by bob1

SDS-PAGE and Western Blotting

  • 1,208 topics
  • 4,057 replies
Western-No bands for sample... - last post by Artemis2007


Discussions on antibody, immunohistochemistry and more...

  • 1,684 topics
  • 2,772 replies
Autoimmune condition as ?co... - last post by mikanaimark


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,116 topics
  • 3,243 replies
Is the a way to detect whic... - last post by DisplayName

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 228 topics
  • 349 replies
List of gene based on expre... - last post by bob1

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 623 topics
  • 1,667 replies
Methylight PCR issues - last post by RKA

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 349 topics
  • 989 replies
Variable ChiP-qPCR even in... - last post by Sandeep Gupta

Macroarray and Microarray

Topics related to different array techniques

  • 129 topics
  • 234 replies
online microarray data - last post by LizRepositive


Questions related to neuroscience and other neuro-related fields

  • 194 topics
  • 269 replies
How do photoreceptors disti... - last post by Trof

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 185 topics
  • 419 replies
Is it possible to cause mor... - last post by molbiolnew

Botany and Plant Biology

Topics specific to plants.

  • 117 topics
  • 281 replies
In vitro culture saffron - last post by mdfenko

Pharmacology and Pharmaceutics

  • 84 topics
  • 164 replies
Crosslinking with glutarald... - last post by labtastic

Histology and Pathology

  • 167 topics
  • 210 replies
Disrupting biofilm formatio... - last post by Cameron


  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof


  • 65 topics
  • 168 replies
Ethyl alcohol dehydration - last post by Aida shaer

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