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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 2,016 topics
  • 6,282 replies
RNA concentration weight an... - last post by itmaybe

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 676 topics
  • 1,228 replies
TCGA and METABRIC- breast c... - last post by BioPe


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 531 topics
  • 1,135 replies
Peroxidase assay - last post by Zagami Francesco

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 148 topics
  • 366 replies
Design Format - last post by Stephbaker

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,261 topics
  • 3,895 replies
Heart specific biomarker - last post by aqua00

Tissue and Cell Culture

  • 1,845 topics
  • 4,042 replies
what is mean by Mock cell? - last post by pito

Stem Cell

  • 139 topics
  • 212 replies
Differentiation of human co... - last post by Stephbaker

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 436 topics
  • 867 replies
Isotype control vs. stained... - last post by Thomson

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,307 topics
  • 13,209 replies
Oligo Molarity - last post by bob1

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,993 topics
  • 6,570 replies
Choosing a common exon in G... - last post by Natalia KM

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,379 topics
  • 6,479 replies
gain optimization closest m... - last post by nightingale

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 668 topics
  • 1,392 replies
urinary exosomal miRNA - last post by SF_HK

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,134 topics
  • 4,179 replies
blood contamination - last post by Zagami Francesco

Protein Expression and Purification

  • 832 topics
  • 2,059 replies
How to eliminate inherent p... - last post by mdfenko

SDS-PAGE and Western Blotting

  • 1,217 topics
  • 4,091 replies
How Best to Clean the Sonic... - last post by Cascadehealthcare


Discussions on antibody, immunohistochemistry and more...

  • 1,692 topics
  • 2,781 replies
Antibody conjugation to COO... - last post by mdfenko


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,133 topics
  • 3,285 replies
How to make an empty vector... - last post by OldCloner

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 230 topics
  • 350 replies
molecular biology - last post by mdfenko

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 624 topics
  • 1,667 replies
"Binomial" and its... - last post by MindlessJPEG

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 351 topics
  • 991 replies
NGS sequencing and capture... - last post by genetics3

Macroarray and Microarray

Topics related to different array techniques

  • 132 topics
  • 237 replies
understanding array - last post by mdfenko


Questions related to neuroscience and other neuro-related fields

  • 196 topics
  • 270 replies
What stimuli trigger hippoc... - last post by electros

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 186 topics
  • 419 replies
Locating the Mouse AV Node - last post by Gray.MA

Botany and Plant Biology

Topics specific to plants.

  • 121 topics
  • 283 replies
Chlorophyll and other pigme... - last post by AdOl

Pharmacology and Pharmaceutics

  • 86 topics
  • 165 replies
Calcium Imaging Calibration - last post by CristianaCris

Histology and Pathology

  • 172 topics
  • 217 replies
Tissue type identification - last post by fionabingly


  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof


  • 67 topics
  • 172 replies
Managing Gibbs free energy - last post by bob1

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