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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,950 topics
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Faint band and primer dimer... - last post by merlav

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 655 topics
  • 1,198 replies
Three experiments with thre... - last post by DRT


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 523 topics
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Doubts - ABTS Radical Catio... - last post by Zagami Francesco

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 141 topics
  • 360 replies
How do we know a study is e... - last post by hobglobin

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,246 topics
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3D Live Cell Imaging - call... - last post by Nanolive

Tissue and Cell Culture

  • 1,814 topics
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3D Live Cell Imaging - call... - last post by Nanolive

Stem Cell

  • 140 topics
  • 212 replies
Stem cell imaging without a... - last post by Nanolive

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 427 topics
  • 863 replies
FAC Sorting of live cells b... - last post by neuropath

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,269 topics
  • 13,157 replies
YFP vs GFP tag - last post by labtastic

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,975 topics
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Determining optimal digesti... - last post by Zagami Francesco

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,345 topics
  • 6,408 replies
RAPD PCR - last post by GradMicro

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 664 topics
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We need a way to see microR... - last post by Fredreload

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,116 topics
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how to predict transmembran... - last post by El Crazy Xabi

Protein Expression and Purification

  • 820 topics
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S. aureus lysis protocol fo... - last post by firefly2280

SDS-PAGE and Western Blotting

  • 1,200 topics
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Forgot to block after strip... - last post by mdfenko


Discussions on antibody, immunohistochemistry and more...

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3DLiveCellImaging -call for... - last post by Nanolive


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,102 topics
  • 3,199 replies
How much protein in S. aureus? - last post by firefly2280

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 225 topics
  • 347 replies
TCGA-the cancer genome atlas - last post by bob1

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 621 topics
  • 1,664 replies
Different efficiencies for... - last post by eyes

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 349 topics
  • 989 replies
Variable ChiP-qPCR even in... - last post by Sandeep Gupta

Macroarray and Microarray

Topics related to different array techniques

  • 129 topics
  • 234 replies
online microarray data - last post by LizRepositive


Questions related to neuroscience and other neuro-related fields

  • 193 topics
  • 267 replies
What is the best dopaminerg... - last post by Katlnz

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 185 topics
  • 419 replies
Is it possible to cause mor... - last post by molbiolnew

Botany and Plant Biology

Topics specific to plants.

  • 117 topics
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In vitro culture saffron - last post by mdfenko

Pharmacology and Pharmaceutics

  • 84 topics
  • 164 replies
Crosslinking with glutarald... - last post by labtastic

Histology and Pathology

  • 158 topics
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unusual FITC background sta... - last post by biochem06


  • 33 topics
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Freezing PBMCs that had bee... - last post by Trof


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solid phase extraction cort... - last post by mdfenko

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