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Protocols and Techniques Forums


Subforums

General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,507 topics
  • 4,923 replies
How do you reduce crystal v... - last post by ajohnson926

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 497 topics
  • 920 replies
How to calculate D' and... - last post by jamestoon1

Biochemistry

Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 407 topics
  • 868 replies
Pestatin A and peptide adso... - last post by labtastic

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 102 topics
  • 263 replies
GC-MS method - last post by abbey

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,048 topics
  • 3,578 replies
Bioassay of G-CSF using MNF... - last post by Winner

Tissue and Cell Culture

  • 1,372 topics
  • 3,150 replies
Autoclaving water to go int... - last post by Enoch Oblitey

Stem Cell

  • 116 topics
  • 182 replies
Selection of transduced LSK... - last post by sssss

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 333 topics
  • 724 replies
CD14+CD16++ monocyte Flow C... - last post by chaghaleh

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 5,687 topics
  • 11,670 replies
Quant-iT assay development... - last post by Ian L

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,561 topics
  • 5,192 replies
Promoter distance from ATG - last post by Glynn

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 1,877 topics
  • 5,254 replies
site-directed mutagenesis p... - last post by phage434

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 569 topics
  • 1,237 replies
Trouble with shRNA transfec... - last post by Chtinico

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 1,901 topics
  • 3,657 replies
concentation calculations - last post by mdfenko

Protein Expression and Purification

  • 633 topics
  • 1,568 replies
adding Tween80 to bacterial... - last post by labtastic

SDS-PAGE and Western Blotting

  • 992 topics
  • 3,342 replies
fused bands of beta-actin i... - last post by birdyeagle

Immunology

Discussions on antibody, immunohistochemistry and more...

  • 1,511 topics
  • 2,441 replies
Efficacy of expired kit sin... - last post by Fujie Derek Koh

Microbiology

Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 853 topics
  • 2,313 replies
Difference between wild-typ... - last post by Mindologist

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 150 topics
  • 255 replies
Genomic DNA and plasmid DNA... - last post by phage434

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 552 topics
  • 1,417 replies
Bisulphite conversion - last post by phage434

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 263 topics
  • 865 replies
Low DNA concentration for s... - last post by pito

Macroarray and Microarray

Topics related to different array techniques

  • 104 topics
  • 184 replies
How do you compare expressi... - last post by mdfenko

Neuroscience

Questions related to neuroscience and other neuro-related fields

  • 170 topics
  • 240 replies
brain slices - last post by notjustalabtech

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 150 topics
  • 359 replies
how to mark and identify mi... - last post by notjustalabtech

Botany and Plant Biology

Topics specific to plants.

  • 86 topics
  • 190 replies
Poor agrobacterium growth f... - last post by hall_b

Pharmacology and Pharmaceutics

  • 68 topics
  • 124 replies
How to calculate drug amoun... - last post by rhombus

Histology and Pathology

  • 94 topics
  • 142 replies
Fixed frozen mouse brain se... - last post by notjustalabtech

Hematology

  • 24 topics
  • 57 replies
about colony assay - last post by atashia

Chemistry

  • 43 topics
  • 112 replies
calculation of salts in pot... - last post by mdfenko



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