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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,927 topics
  • 6,104 replies
Transfer buffer with methan... - last post by mdfenko

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 649 topics
  • 1,193 replies
Database of only characteri... - last post by rmbio


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 514 topics
  • 1,080 replies
Albumin isoforms - last post by biochem06

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 139 topics
  • 357 replies
A contagious man infecting... - last post by bob1

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,238 topics
  • 3,867 replies
Fusion protein instability... - last post by miST32

Tissue and Cell Culture

  • 1,799 topics
  • 3,971 replies
Make samples from sera on A... - last post by SelcenC

Stem Cell

  • 138 topics
  • 211 replies
Stem Cell Donation - last post by Chloe Mica

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 420 topics
  • 859 replies
Help with the Red Blood Cel... - last post by bob1

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,236 topics
  • 13,096 replies
transfection - last post by bob1

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,963 topics
  • 6,480 replies
m13 blue white screening - last post by Paula N

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,322 topics
  • 6,372 replies
Same primers and different... - last post by sergio_pv

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 658 topics
  • 1,371 replies
negative control miRNA - last post by bob1

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,108 topics
  • 4,134 replies
Flow Cytometry vs Western B... - last post by bob1

Protein Expression and Purification

  • 802 topics
  • 1,994 replies
Pros and Cons of refolding... - last post by Nancy.Lee

SDS-PAGE and Western Blotting

  • 1,191 topics
  • 4,027 replies
Biorad Miniprotean review - last post by bob1


Discussions on antibody, immunohistochemistry and more...

  • 1,669 topics
  • 2,756 replies
Can microglia stain positiv... - last post by neuropath


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,094 topics
  • 3,184 replies
Help - preserving bacteria... - last post by OxfordTechnician

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 218 topics
  • 344 replies
Finding promoter regions - last post by Ken S

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 620 topics
  • 1,661 replies
ChIP-qPCR, calculating % input - last post by Valie15

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 347 topics
  • 989 replies
Radiactive PCR for ChIP qua... - last post by autophagator

Macroarray and Microarray

Topics related to different array techniques

  • 129 topics
  • 233 replies
pesticides - last post by 827753


Questions related to neuroscience and other neuro-related fields

  • 192 topics
  • 267 replies
What is EPSP slope ? - last post by Inmost sun

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 184 topics
  • 418 replies
Free software to analyze IV... - last post by neuropath

Botany and Plant Biology

Topics specific to plants.

  • 116 topics
  • 280 replies
What does "R.H. 1619... - last post by nbic

Pharmacology and Pharmaceutics

  • 82 topics
  • 156 replies
Preparation of antibiotic disc - last post by mdfenko

Histology and Pathology

  • 157 topics
  • 202 replies
IHC Protein Competition, Pr... - last post by bob1


  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof


  • 59 topics
  • 158 replies
EDTA precipitates out of ED... - last post by mdfenko

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