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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,766 topics
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Separation of glycerol from... - last post by saher

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 587 topics
  • 1,116 replies
How to analyze metabolomics... - last post by sialic acid


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 470 topics
  • 982 replies
The project title is ôStudi... - last post by bob1

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 118 topics
  • 316 replies
new trends in rice science - last post by pito

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,166 topics
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can anyone tell me what kin... - last post by bob1

Tissue and Cell Culture

  • 1,639 topics
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Plastic fibres (from the pi... - last post by Tabaluga

Stem Cell

  • 134 topics
  • 200 replies
Totipotent Stem Cell From M... - last post by caters

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 387 topics
  • 815 replies
Viability dye 7-AAD - last post by Tabaluga

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,027 topics
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Changing the vector - last post by RNA woman

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,832 topics
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Lac Promoter activity in P.... - last post by clindsaym

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,161 topics
  • 6,019 replies
Overlapping PCR, need help - last post by Paniel

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 620 topics
  • 1,320 replies
lincRNA-p21 sequence - last post by Martin Faber

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,018 topics
  • 3,928 replies
Trouble with membrane assoc... - last post by ghghghghgh

Protein Expression and Purification

  • 724 topics
  • 1,796 replies
EGFP expression - last post by Suvash

SDS-PAGE and Western Blotting

  • 1,090 topics
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Problem with thawed samples - last post by steinpdh


Discussions on antibody, immunohistochemistry and more...

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Using CPG-ODN 1826 in guine... - last post by Mariam


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 999 topics
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Can anyone help me to check... - last post by Celz

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 195 topics
  • 324 replies
Examples of short RNAs that... - last post by moonfleet

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 600 topics
  • 1,607 replies
MNase assay - how to quanti... - last post by phage434

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 318 topics
  • 952 replies
ChIP: Inconsistent reverse... - last post by shp952

Macroarray and Microarray

Topics related to different array techniques

  • 120 topics
  • 219 replies
Array Data for tumours (lun... - last post by Ahyi


Questions related to neuroscience and other neuro-related fields

  • 183 topics
  • 257 replies
Questions on mind uploading - last post by bob1

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 178 topics
  • 406 replies
CpG injection for IFNalpha... - last post by BartL

Botany and Plant Biology

Topics specific to plants.

  • 104 topics
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problems on the media for o... - last post by shinome

Pharmacology and Pharmaceutics

  • 75 topics
  • 143 replies
What is the best statisical... - last post by DRT

Histology and Pathology

  • 126 topics
  • 175 replies
Spotted DAB staining! - last post by Ambinlab


  • 32 topics
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how to make Percoll solutio... - last post by mdfenko


  • 57 topics
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Acid-Base Buffer Question - last post by bob1

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