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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,819 topics
  • 5,836 replies
Agarose gel for determining... - last post by bleep5

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 606 topics
  • 1,140 replies
Power Analyses - last post by Alternative


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 480 topics
  • 1,001 replies
RNA extraction and DNase tr... - last post by Kamil

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 130 topics
  • 333 replies
where do you get cells need... - last post by animodel123

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,191 topics
  • 3,815 replies
I stored my PVDF membrane a... - last post by GNANA

Tissue and Cell Culture

  • 1,694 topics
  • 3,826 replies
Dislodge cell in 96well plate - last post by ahmadfathi

Stem Cell

  • 136 topics
  • 204 replies
Problem in mouse embryonic... - last post by dhanur

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 403 topics
  • 846 replies
Very high background, or no... - last post by mkimx255

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,101 topics
  • 12,876 replies
Gel doubling banding and re... - last post by bob1

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,887 topics
  • 6,253 replies
Trouble with PCR using liga... - last post by katinka.apagyi

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,210 topics
  • 6,136 replies
Reference Gene/HKG- every p... - last post by bob1

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 637 topics
  • 1,345 replies
Can I use housekeeper like... - last post by -hiu-

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,046 topics
  • 3,989 replies
Trouble eluting all protein... - last post by neuron

Protein Expression and Purification

  • 747 topics
  • 1,831 replies
Anion Exchange Buffers - last post by labtastic

SDS-PAGE and Western Blotting

  • 1,126 topics
  • 3,826 replies
Troubleshooting for BN PAGE - last post by mdfenko


Discussions on antibody, immunohistochemistry and more...

  • 1,634 topics
  • 2,681 replies
concentration of samples wi... - last post by sgt4boston


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,024 topics
  • 2,981 replies
unpredictable results in gr... - last post by bob1

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 199 topics
  • 325 replies
Mutation rate - last post by p.blaze53

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 603 topics
  • 1,612 replies
Online tools for methylatio... - last post by rakun

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 329 topics
  • 969 replies
Fixing cells in 70% Ethanol... - last post by Teri

Macroarray and Microarray

Topics related to different array techniques

  • 125 topics
  • 226 replies
online microarray data - last post by Srilatha Dharmavaram


Questions related to neuroscience and other neuro-related fields

  • 188 topics
  • 265 replies
Immunocytochemistry neurons... - last post by bob1

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 181 topics
  • 413 replies
Electrically charged insect... - last post by hobglobin

Botany and Plant Biology

Topics specific to plants.

  • 109 topics
  • 261 replies
Help to sterilize unknown seed - last post by harrypotter

Pharmacology and Pharmaceutics

  • 78 topics
  • 145 replies
introducing alkyne or azide... - last post by Hehehe

Histology and Pathology

  • 137 topics
  • 185 replies
Help ID fluoescently-labell... - last post by MC_london


  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof


  • 57 topics
  • 152 replies
5 M NaCl stock - last post by Ahyi

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