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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 2,037 topics
  • 6,311 replies
Storing plasmid at 4 degree... - last post by alexsunny123

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 685 topics
  • 1,235 replies
How to statistically/bioinf... - last post by bob1


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 532 topics
  • 1,140 replies
Rotating magnetic fields ag... - last post by sciencetoolbar

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 148 topics
  • 366 replies
Design Format - last post by Stephbaker

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,266 topics
  • 3,901 replies
Evaluation of cell migratio... - last post by Biochemistica

Tissue and Cell Culture

  • 1,847 topics
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Fibroblast Cell Culture Iss... - last post by Afrah

Stem Cell

  • 139 topics
  • 212 replies
Differentiation of human co... - last post by Stephbaker

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 436 topics
  • 867 replies
Isotype control vs. stained... - last post by Thomson

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,328 topics
  • 13,240 replies
Cloning a zebrafish gene? - last post by bob1

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,997 topics
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TA cloning for sars cov 2 n... - last post by akremon

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,387 topics
  • 6,489 replies
Standard curve - copy numbe... - last post by Thyago

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 671 topics
  • 1,392 replies
Help in gene editing - last post by tesla2020

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,139 topics
  • 4,185 replies
eliminating albumin from sa... - last post by mdfenko

Protein Expression and Purification

  • 833 topics
  • 2,061 replies
Virus vs protein purification - last post by jamesholder

SDS-PAGE and Western Blotting

  • 1,219 topics
  • 4,098 replies
PVDF Membrane Floating in R... - last post by mdfenko


Discussions on antibody, immunohistochemistry and more...

  • 1,695 topics
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ELISA a biosensor? - last post by bob1


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,139 topics
  • 3,312 replies
Inhibition of SARS-CoV-2 in... - last post by Phil Geis

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 231 topics
  • 350 replies
Eukariotic Linear Motifs - last post by Ziv

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 624 topics
  • 1,667 replies
"Binomial" and its... - last post by MindlessJPEG

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 352 topics
  • 991 replies
Sample storage during ChIP... - last post by Artemis2007

Macroarray and Microarray

Topics related to different array techniques

  • 133 topics
  • 237 replies
autoantibody array - last post by SF_HK


Questions related to neuroscience and other neuro-related fields

  • 196 topics
  • 272 replies
Immunofluorescence quantifi... - last post by Inmost sun

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 186 topics
  • 419 replies
Locating the Mouse AV Node - last post by Gray.MA

Botany and Plant Biology

Topics specific to plants.

  • 122 topics
  • 283 replies
Germination test - last post by bioxesus

Pharmacology and Pharmaceutics

  • 86 topics
  • 165 replies
Calcium Imaging Calibration - last post by CristianaCris

Histology and Pathology

  • 173 topics
  • 217 replies
Antigen Retrieval - last post by SAT142


  • 33 topics
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Freezing PBMCs that had bee... - last post by Trof


  • 67 topics
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Managing Gibbs free energy - last post by bob1

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