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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,829 topics
  • 5,884 replies
Dissolving solid reagent in... - last post by kusuma

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 608 topics
  • 1,141 replies
bioinformatic books - last post by mdfenko


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 485 topics
  • 1,020 replies
Pull-Down Assay, EMSA Assay... - last post by mdfenko

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 132 topics
  • 339 replies
What method is the best to... - last post by lilywong1991

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,197 topics
  • 3,821 replies
U937 Differentiation Adherence - last post by SICA

Tissue and Cell Culture

  • 1,717 topics
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Is there much use for anima... - last post by TheFOrsh

Stem Cell

  • 136 topics
  • 207 replies
Problem in mouse embryonic... - last post by KirstenOrion

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 409 topics
  • 846 replies
Which markers to distinguis... - last post by EvelynMDC

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,121 topics
  • 12,909 replies
Separation of biotinylated... - last post by dtmulder

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,904 topics
  • 6,300 replies
replicating a mammalian exp... - last post by phage434

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,226 topics
  • 6,167 replies
RT-PCR Amplification failures - last post by Kanai Emmanuel Thomas

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 639 topics
  • 1,347 replies
cell viability effect and i... - last post by bongiwoman

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,061 topics
  • 4,018 replies
What post-translational mod... - last post by pavoni.ernesto

Protein Expression and Purification

  • 753 topics
  • 1,855 replies
protein expression site - last post by Samantha965

SDS-PAGE and Western Blotting

  • 1,135 topics
  • 3,854 replies
White bands in western blot... - last post by mdfenko


Discussions on antibody, immunohistochemistry and more...

  • 1,643 topics
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What is best to measure pro... - last post by mdfenko


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,035 topics
  • 3,007 replies
Efficient lysis of E.coli c... - last post by phage434

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 205 topics
  • 331 replies
frequency of mitochondrial... - last post by OA17

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 605 topics
  • 1,614 replies
MSP_Primer dimers? - last post by 5280

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 330 topics
  • 969 replies
Role of RNA Sequencing Prim... - last post by cellculture_newbie

Macroarray and Microarray

Topics related to different array techniques

  • 125 topics
  • 226 replies
online microarray data - last post by Srilatha Dharmavaram


Questions related to neuroscience and other neuro-related fields

  • 188 topics
  • 265 replies
Immunocytochemistry neurons... - last post by bob1

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 182 topics
  • 416 replies
Routine medical tests on mi... - last post by pito

Botany and Plant Biology

Topics specific to plants.

  • 110 topics
  • 262 replies
tomato transformation - bas... - last post by cahyaprihatna

Pharmacology and Pharmaceutics

  • 80 topics
  • 147 replies
Cell lines behaving differe... - last post by DRT

Histology and Pathology

  • 139 topics
  • 188 replies
LUNA Staining Protocol to d... - last post by Empath


  • 33 topics
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Freezing PBMCs that had bee... - last post by Trof


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5 M NaCl stock - last post by Ahyi

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