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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,842 topics
  • 5,916 replies
0.4M phosphate buffer ph 6.8 - last post by mdfenko

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 614 topics
  • 1,145 replies
Filtering/cutting fastq by... - last post by mboss


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 488 topics
  • 1,025 replies
Creatinine level is high ev... - last post by sayk

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 133 topics
  • 339 replies
comprehensive search for a... - last post by phdgirl

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,206 topics
  • 3,834 replies
control shRNA - last post by Lee6948

Tissue and Cell Culture

  • 1,728 topics
  • 3,880 replies
Help with HUVEC cell transf... - last post by Bonjo7

Stem Cell

  • 137 topics
  • 210 replies
PCR DIG-labeling kit preblem? - last post by bob1

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 410 topics
  • 846 replies
How to clean up cell fragme... - last post by SoggyJaw

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,143 topics
  • 12,941 replies
IgG control for IP - last post by bob1

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,916 topics
  • 6,357 replies
Weird results with bacteria... - last post by rmbio

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,250 topics
  • 6,247 replies
Standardizing cDNA concentr... - last post by Sakumi

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 642 topics
  • 1,349 replies
scanning miRNA/piRNA/siRNA... - last post by zhxq09

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,067 topics
  • 4,030 replies
RECOMBINANT PROTEIN - last post by Chomolungma

Protein Expression and Purification

  • 761 topics
  • 1,876 replies
Help with Superdex200 incre... - last post by labtastic

SDS-PAGE and Western Blotting

  • 1,144 topics
  • 3,895 replies
37kb and 7kb protein in wes... - last post by kegaff


Discussions on antibody, immunohistochemistry and more...

  • 1,648 topics
  • 2,697 replies
Avoid drying out of coversl... - last post by mdfenko


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,044 topics
  • 3,033 replies
Morbidostat - last post by neuron

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 208 topics
  • 332 replies
How to stimulate hepatocyte... - last post by CandyTon

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 610 topics
  • 1,644 replies
MSP_Primer dimers? - last post by methylnick

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 333 topics
  • 972 replies
MiSeq Reporter program - last post by moerae

Macroarray and Microarray

Topics related to different array techniques

  • 125 topics
  • 226 replies
online microarray data - last post by Srilatha Dharmavaram


Questions related to neuroscience and other neuro-related fields

  • 190 topics
  • 265 replies
need help with nerves and r... - last post by sirius

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 182 topics
  • 416 replies
Routine medical tests on mi... - last post by pito

Botany and Plant Biology

Topics specific to plants.

  • 111 topics
  • 270 replies
Microalgae count problem - last post by radioactivestar88

Pharmacology and Pharmaceutics

  • 80 topics
  • 150 replies
Preferred assay for measuri... - last post by DRT

Histology and Pathology

  • 139 topics
  • 188 replies
LUNA Staining Protocol to d... - last post by Empath


  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof


  • 57 topics
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5 M NaCl stock - last post by Ahyi

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