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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,790 topics
  • 5,778 replies
Passaging cells - last post by Biochem_newbie

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 598 topics
  • 1,130 replies
Different transcriptional a... - last post by Tabaluga


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 476 topics
  • 994 replies
Inorganic phosphorous - last post by Zagami Francesco

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 121 topics
  • 317 replies
Are you a scientist/invento... - last post by jobscallumco

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,174 topics
  • 3,786 replies
Detection of Td-Tomato prot... - last post by Rsm

Tissue and Cell Culture

  • 1,672 topics
  • 3,775 replies
Transducing HEK293 cells wi... - last post by ccsusx

Stem Cell

  • 136 topics
  • 204 replies
Problem in mouse embryonic... - last post by dhanur

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 393 topics
  • 825 replies
Basic questions - last post by Tabaluga

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,065 topics
  • 12,785 replies
ABC(D) Model of Plant Flowers - last post by Tom1991

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,859 topics
  • 6,183 replies
Need help in codon optimiza... - last post by astroboy89

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,184 topics
  • 6,058 replies
I need help with PCR problems - last post by bob1

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 628 topics
  • 1,329 replies
about microrna overexpressing - last post by itsron

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,039 topics
  • 3,977 replies
Large protein removal (frac... - last post by TurtleBob

Protein Expression and Purification

  • 736 topics
  • 1,813 replies
Elute fractions that have t... - last post by trungnghiatn

SDS-PAGE and Western Blotting

  • 1,109 topics
  • 3,752 replies
storing membrane - last post by mdfenko


Discussions on antibody, immunohistochemistry and more...

  • 1,623 topics
  • 2,661 replies
Is there always a presence... - last post by bob1


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,015 topics
  • 2,955 replies
"luxury" microbiolo... - last post by phage434

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 197 topics
  • 324 replies
Genome annotation - last post by Dav

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 602 topics
  • 1,607 replies
housekeeping/reference gene... - last post by Lj Lee

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 324 topics
  • 956 replies
Amplicons detected for nega... - last post by trungbiotech

Macroarray and Microarray

Topics related to different array techniques

  • 123 topics
  • 224 replies
DETERMINATION OF BASELINE F... - last post by Nyameba


Questions related to neuroscience and other neuro-related fields

  • 185 topics
  • 260 replies
Neurophysiology- NERVE onli... - last post by TheForumLord

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 179 topics
  • 411 replies
female BALB/c mice eating a... - last post by VarianWrynn

Botany and Plant Biology

Topics specific to plants.

  • 106 topics
  • 250 replies
Is it possible to stored en... - last post by hobglobin

Pharmacology and Pharmaceutics

  • 77 topics
  • 143 replies
Ebola Virus - last post by Einfolge

Histology and Pathology

  • 129 topics
  • 177 replies
Protocole Immunohistochemis... - last post by orianedl


  • 32 topics
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how to make Percoll solutio... - last post by mdfenko


  • 57 topics
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5 M NaCl stock - last post by Ahyi

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