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Protocols and Techniques Forums


Subforums

General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,493 topics
  • 4,890 replies
cell seeded 3D collagen 1 gel - last post by GMARATHA

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 492 topics
  • 914 replies
Protein structure modeling... - last post by GNANA

Biochemistry

Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 400 topics
  • 851 replies
protein fractionation - last post by labtastic

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 101 topics
  • 263 replies
Launching a new Influenza F... - last post by Mohamed samir

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,039 topics
  • 3,570 replies
anyone has experience in ad... - last post by gyma

Tissue and Cell Culture

  • 1,358 topics
  • 3,114 replies
SH-SY5Y and Poly-L-Lysine - last post by BRD

Stem Cell

  • 114 topics
  • 182 replies
problems in culturing mice... - last post by mesenchymal stem cell

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 329 topics
  • 721 replies
PI stain for cell viability... - last post by rpjkmust916

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 5,664 topics
  • 11,585 replies
Point mutations in synthesi... - last post by Trof

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,537 topics
  • 5,099 replies
"Easiest" sticky en... - last post by doxorubicin

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 1,847 topics
  • 5,181 replies
Normalization of Ct of inte... - last post by Tzia

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 563 topics
  • 1,228 replies
which to use: miR or miRexp... - last post by mahrak

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 1,889 topics
  • 3,639 replies
2D Image Primary Cell line - last post by mdfenko

Protein Expression and Purification

  • 627 topics
  • 1,548 replies
Problems in expression of t... - last post by pDNA

SDS-PAGE and Western Blotting

  • 969 topics
  • 3,260 replies
Western Blot - Quantifying... - last post by Iceage

Immunology

Discussions on antibody, immunohistochemistry and more...

  • 1,505 topics
  • 2,434 replies
Antibody reactivity - last post by thanhlt

Microbiology

Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 847 topics
  • 2,298 replies
Deactivation of bacteria fo... - last post by El Crazy Xabi

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 148 topics
  • 244 replies
GHR gene location of water... - last post by iamleos

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 549 topics
  • 1,411 replies
experiments related to epig... - last post by DeniseB

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 253 topics
  • 858 replies
ChIP, Sonication and SDS - last post by j47027

Macroarray and Microarray

Topics related to different array techniques

  • 101 topics
  • 180 replies
QC saga continues.... from... - last post by mdfenko

Neuroscience

Questions related to neuroscience and other neuro-related fields

  • 168 topics
  • 236 replies
Lysis buffer for white matter - last post by Curtis

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 149 topics
  • 347 replies
how to mark and identify mi... - last post by adibah

Botany and Plant Biology

Topics specific to plants.

  • 86 topics
  • 190 replies
Poor agrobacterium growth f... - last post by hall_b

Pharmacology and Pharmaceutics

  • 66 topics
  • 122 replies
Stimulate receptor constitu... - last post by Annasae

Histology and Pathology

  • 94 topics
  • 141 replies
restain secondary antibody - last post by wdyeo

Hematology

  • 24 topics
  • 57 replies
about colony assay - last post by atashia

Chemistry

  • 42 topics
  • 107 replies
Possible substitutions of H... - last post by Annasae



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