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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,752 topics
  • 5,675 replies
Measuring iNOS protein in l... - last post by bbasok

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 583 topics
  • 1,105 replies
Affymatrix Microarray data - last post by CD Genomics


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 468 topics
  • 981 replies
Glycolysis oxidation states - last post by bob1

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 118 topics
  • 316 replies
new trends in rice science - last post by pito

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,162 topics
  • 3,757 replies
Karyotyping, Giemsa Staining - last post by Ant_Ony

Tissue and Cell Culture

  • 1,621 topics
  • 3,680 replies
C2C12 myotube contraction - last post by SusieQ

Stem Cell

  • 134 topics
  • 200 replies
Totipotent Stem Cell From M... - last post by caters

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 385 topics
  • 807 replies
Biotin-streptavidin signal... - last post by JGBarin

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,008 topics
  • 12,639 replies
Restriction digestion and CIP - last post by phage434

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,822 topics
  • 6,079 replies
RBS - last post by yusii

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,150 topics
  • 5,999 replies
3' RACE  troubles - last post by Ant_Ony

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 619 topics
  • 1,319 replies
prediction of microRNA targ... - last post by pcrman

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,009 topics
  • 3,912 replies
Choosing MWCO For Dialysis... - last post by Artemis2007

Protein Expression and Purification

  • 720 topics
  • 1,792 replies
i am trying to get rid of u... - last post by labtastic

SDS-PAGE and Western Blotting

  • 1,084 topics
  • 3,690 replies
How many nitrocellulose blo... - last post by bob1


Discussions on antibody, immunohistochemistry and more...

  • 1,607 topics
  • 2,625 replies
Good clones no bindres - last post by Ali 33


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 991 topics
  • 2,908 replies
Will S. griseus grow on Col... - last post by Adriana Reis

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 191 topics
  • 321 replies
Math World Genetics - last post by caters

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 597 topics
  • 1,596 replies
Finding methylation in the... - last post by saravana devi

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 316 topics
  • 947 replies
Mock IgG versus No Antibody... - last post by yy99875

Macroarray and Microarray

Topics related to different array techniques

  • 120 topics
  • 219 replies
Array Data for tumours (lun... - last post by Ahyi


Questions related to neuroscience and other neuro-related fields

  • 182 topics
  • 257 replies
Mice LTP is not working - last post by Inmost sun

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 175 topics
  • 406 replies
orthotopic mouse model of U... - last post by Fungus_Dreams

Botany and Plant Biology

Topics specific to plants.

  • 102 topics
  • 242 replies
Why would you use DNase as... - last post by sara_molecular

Pharmacology and Pharmaceutics

  • 75 topics
  • 143 replies
What is the best statisical... - last post by DRT

Histology and Pathology

  • 123 topics
  • 174 replies
B-gal staining troubleshoot... - last post by Irina_Vavi


  • 32 topics
  • 78 replies
Unusual Buffy Coat - (I Think) - last post by djvan


  • 57 topics
  • 149 replies
Acid-Base Buffer Question - last post by bob1

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