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Protocols and Techniques Forums


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General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,751 topics
  • 5,675 replies
Molecular Biologist in soil... - last post by pito

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 583 topics
  • 1,105 replies
Affymatrix Microarray data - last post by CD Genomics

Biochemistry

Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 465 topics
  • 976 replies
Pectinase Enzyme Assay - last post by mdfenko

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 118 topics
  • 316 replies
new trends in rice science - last post by pito

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,161 topics
  • 3,757 replies
MTT assay without positive... - last post by vinclam

Tissue and Cell Culture

  • 1,621 topics
  • 3,678 replies
Sealing hole around tubing... - last post by bob1

Stem Cell

  • 134 topics
  • 197 replies
how to transport stem cells... - last post by RLAD

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 385 topics
  • 807 replies
Biotin-streptavidin signal... - last post by JGBarin

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,005 topics
  • 12,628 replies
Soil Metagenome extraction - last post by Eslam Samir

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,820 topics
  • 6,064 replies
Gel for my ligation - last post by bob1

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,146 topics
  • 5,989 replies
Best Taq for colony PCR? - last post by Trof

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 619 topics
  • 1,319 replies
prediction of microRNA targ... - last post by pcrman

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,009 topics
  • 3,912 replies
Choosing MWCO For Dialysis... - last post by Artemis2007

Protein Expression and Purification

  • 719 topics
  • 1,789 replies
CO- IP for nuclear protein - last post by jaya2020

SDS-PAGE and Western Blotting

  • 1,084 topics
  • 3,690 replies
How many nitrocellulose blo... - last post by bob1

Immunology

Discussions on antibody, immunohistochemistry and more...

  • 1,606 topics
  • 2,625 replies
Preparation of antibody column - last post by mdfenko

Microbiology

Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 989 topics
  • 2,904 replies
Problems with M13 phage - last post by bob1

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 190 topics
  • 317 replies
Mutation screening - last post by bob1

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 597 topics
  • 1,596 replies
Finding methylation in the... - last post by saravana devi

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 315 topics
  • 946 replies
ChIP sonication problem - last post by ap83

Macroarray and Microarray

Topics related to different array techniques

  • 120 topics
  • 219 replies
Array Data for tumours (lun... - last post by Ahyi

Neuroscience

Questions related to neuroscience and other neuro-related fields

  • 182 topics
  • 257 replies
Mice LTP is not working - last post by Inmost sun

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 175 topics
  • 406 replies
orthotopic mouse model of U... - last post by Fungus_Dreams

Botany and Plant Biology

Topics specific to plants.

  • 102 topics
  • 242 replies
Why would you use DNase as... - last post by sara_molecular

Pharmacology and Pharmaceutics

  • 75 topics
  • 143 replies
What is the best statisical... - last post by DRT

Histology and Pathology

  • 123 topics
  • 174 replies
B-gal staining troubleshoot... - last post by Irina_Vavi

Hematology

  • 31 topics
  • 76 replies
RNA isolation from whole blood - last post by Trof

Chemistry

  • 57 topics
  • 149 replies
Acid-Base Buffer Question - last post by bob1



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