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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,741 topics
  • 5,657 replies
question on calculation DNA... - last post by mlomonaco

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 582 topics
  • 1,103 replies
How to run genscan on a lar... - last post by Garima


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 462 topics
  • 969 replies
Fatty Acid Synthesis - last post by Tabaluga

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 116 topics
  • 314 replies
Would someone help me discu... - last post by bob1

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,159 topics
  • 3,748 replies
target genes of transcripti... - last post by bio1681992

Tissue and Cell Culture

  • 1,610 topics
  • 3,663 replies
Which trypsin EDTA to use? - last post by SusieQ

Stem Cell

  • 134 topics
  • 197 replies
how to transport stem cells... - last post by RLAD

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 385 topics
  • 802 replies
Viability dye 7-AAD - last post by Shoooda

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 5,995 topics
  • 12,561 replies
page electrophoresis - last post by mdfenko

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,809 topics
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Transforming dna from agaro... - last post by Trof

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,132 topics
  • 5,949 replies
Plasmid calculation questio... - last post by Trof

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 619 topics
  • 1,319 replies
prediction of microRNA targ... - last post by pcrman

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,006 topics
  • 3,896 replies
Immunoprecipitation trouble... - last post by Artemis2007

Protein Expression and Purification

  • 716 topics
  • 1,782 replies
Problems with a nonspecific... - last post by jessiray189

SDS-PAGE and Western Blotting

  • 1,077 topics
  • 3,661 replies
Western blot with smeared b... - last post by mdfenko


Discussions on antibody, immunohistochemistry and more...

  • 1,603 topics
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Streptavidin binding direct... - last post by tkf


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 980 topics
  • 2,838 replies
Spanish flu - last post by Adriana Reis

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 189 topics
  • 316 replies
need some advise about PCR... - last post by Ameya P

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 593 topics
  • 1,586 replies
Best technique to analyse m... - last post by phage434

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 314 topics
  • 945 replies
Purify ChIP DNA using Qiage... - last post by Trevaly

Macroarray and Microarray

Topics related to different array techniques

  • 120 topics
  • 219 replies
Array Data for tumours (lun... - last post by Ahyi


Questions related to neuroscience and other neuro-related fields

  • 182 topics
  • 256 replies
measure of cell size - last post by bob1

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 175 topics
  • 406 replies
orthotopic mouse model of U... - last post by Fungus_Dreams

Botany and Plant Biology

Topics specific to plants.

  • 102 topics
  • 242 replies
Why would you use DNase as... - last post by sara_molecular

Pharmacology and Pharmaceutics

  • 75 topics
  • 143 replies
What is the best statisical... - last post by DRT

Histology and Pathology

  • 122 topics
  • 174 replies
Virtual Slide Software - last post by EAG


  • 31 topics
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Obtaining Serum from frozen... - last post by mdfenko


  • 55 topics
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Does autoclaving salts of m... - last post by p.phadtare

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