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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,857 topics
  • 5,948 replies
pH adjustment of 3.5% NaCl... - last post by phage434

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 619 topics
  • 1,155 replies
Question on blasting sequen... - last post by Jing2


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 490 topics
  • 1,030 replies
Chemical to revert actin ag... - last post by mdfenko

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 133 topics
  • 339 replies
comprehensive search for a... - last post by phdgirl

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,213 topics
  • 3,841 replies
SAOS.2 Cell detachment with... - last post by bob1

Tissue and Cell Culture

  • 1,730 topics
  • 3,891 replies
Fibroblast Cell Culture Iss... - last post by bob1

Stem Cell

  • 138 topics
  • 210 replies
Trouble with definitive end... - last post by Delta

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 414 topics
  • 850 replies
Why GFP/YFP+ neurons couldn... - last post by bob1

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,154 topics
  • 12,986 replies
Any DNA/RNA elements I can... - last post by Ahrenhase

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,922 topics
  • 6,369 replies
pUC and pSC101 vectors in t... - last post by labtastic

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,269 topics
  • 6,272 replies
typical mRNA test with huge... - last post by Dr PCR

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 645 topics
  • 1,352 replies
Reverse transcription for m... - last post by marilia

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,070 topics
  • 4,043 replies
Transfer buffer for tris tr... - last post by bob1

Protein Expression and Purification

  • 765 topics
  • 1,886 replies
Removal of cleaved peptide... - last post by mdfenko

SDS-PAGE and Western Blotting

  • 1,150 topics
  • 3,922 replies
Deformed bands in gel - last post by SusieQ


Discussions on antibody, immunohistochemistry and more...

  • 1,650 topics
  • 2,708 replies
Bone marrow chimera mice di... - last post by Ahrenhase


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,049 topics
  • 3,069 replies
First ever project suggesti... - last post by pito

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 210 topics
  • 336 replies
genotyping choices by scale - last post by angel0071987

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 613 topics
  • 1,648 replies
Personalized methylated hum... - last post by elenagardini

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 333 topics
  • 973 replies
% INPUT - ChIP - last post by leuyen

Macroarray and Microarray

Topics related to different array techniques

  • 126 topics
  • 227 replies
Using Gene Ontology - last post by mdfenko


Questions related to neuroscience and other neuro-related fields

  • 191 topics
  • 265 replies
Why GFP/YFP in neurons is l... - last post by Marinavv

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 182 topics
  • 416 replies
Routine medical tests on mi... - last post by pito

Botany and Plant Biology

Topics specific to plants.

  • 111 topics
  • 270 replies
Microalgae count problem - last post by radioactivestar88

Pharmacology and Pharmaceutics

  • 80 topics
  • 150 replies
Preferred assay for measuri... - last post by DRT

Histology and Pathology

  • 140 topics
  • 188 replies
stomach-oesephagus histology - last post by Valdrin Hajdari


  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof


  • 57 topics
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5 M NaCl stock - last post by Ahyi

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