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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,840 topics
  • 5,908 replies
Percipitate while pH settin... - last post by El Crazy Xabi

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 611 topics
  • 1,144 replies
annotation tool - last post by bensaoud chaima


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 485 topics
  • 1,020 replies
Pull-Down Assay, EMSA Assay... - last post by mdfenko

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 133 topics
  • 339 replies
comprehensive search for a... - last post by phdgirl

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,199 topics
  • 3,823 replies
Distribution of cell recept... - last post by Jon Moulton

Tissue and Cell Culture

  • 1,721 topics
  • 3,863 replies
Combining close passage num... - last post by JDSBlueDevl

Stem Cell

  • 136 topics
  • 207 replies
Problem in mouse embryonic... - last post by KirstenOrion

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 409 topics
  • 846 replies
Which markers to distinguis... - last post by EvelynMDC

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,125 topics
  • 12,916 replies
Are there any safe nucleic... - last post by 5280

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,909 topics
  • 6,326 replies
cloning problems using in-f... - last post by miST32

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,232 topics
  • 6,180 replies
pcr primer design - last post by Mad researcher

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 639 topics
  • 1,347 replies
cell viability effect and i... - last post by bongiwoman

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,061 topics
  • 4,021 replies
What post-translational mod... - last post by miST32

Protein Expression and Purification

  • 756 topics
  • 1,865 replies
Pierce NE-PER final insolub... - last post by 5280

SDS-PAGE and Western Blotting

  • 1,137 topics
  • 3,860 replies
Troubleshooting for BN PAGE - last post by mdfenko


Discussions on antibody, immunohistochemistry and more...

  • 1,644 topics
  • 2,691 replies
Unusual binding of Hybridom... - last post by ibmbio


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,036 topics
  • 3,011 replies
need to make a new glycerol... - last post by ibmbio

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 207 topics
  • 331 replies
SNP arrays (23andme and oth... - last post by Trof

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 606 topics
  • 1,616 replies
MSP_Primer dimers? - last post by elenagardini

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 331 topics
  • 971 replies
Flash freezing ChIP samples... - last post by Dr_Rob

Macroarray and Microarray

Topics related to different array techniques

  • 125 topics
  • 226 replies
online microarray data - last post by Srilatha Dharmavaram


Questions related to neuroscience and other neuro-related fields

  • 188 topics
  • 265 replies
Immunocytochemistry neurons... - last post by bob1

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 182 topics
  • 416 replies
Routine medical tests on mi... - last post by pito

Botany and Plant Biology

Topics specific to plants.

  • 110 topics
  • 263 replies
What plant species are you... - last post by Labmate0910

Pharmacology and Pharmaceutics

  • 81 topics
  • 150 replies
Preferred assay for measuri... - last post by DRT

Histology and Pathology

  • 139 topics
  • 188 replies
LUNA Staining Protocol to d... - last post by Empath


  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof


  • 57 topics
  • 152 replies
5 M NaCl stock - last post by Ahyi

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