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Protocols and Techniques Forums


Subforums

General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,505 topics
  • 4,922 replies
ultracentrifugation trouble - last post by mdfenko

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 497 topics
  • 920 replies
How to calculate D' and... - last post by jamestoon1

Biochemistry

Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 406 topics
  • 866 replies
Testing nucleotide-protein... - last post by mdfenko

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 102 topics
  • 263 replies
GC-MS method - last post by abbey

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,048 topics
  • 3,578 replies
Bioassay of G-CSF using MNF... - last post by Winner

Tissue and Cell Culture

  • 1,370 topics
  • 3,148 replies
Autoclaving water to go int... - last post by madelingirly

Stem Cell

  • 116 topics
  • 182 replies
Selection of transduced LSK... - last post by sssss

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 333 topics
  • 724 replies
CD14+CD16++ monocyte Flow C... - last post by chaghaleh

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 5,686 topics
  • 11,669 replies
In vitro transcription: for... - last post by Lilange

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,561 topics
  • 5,190 replies
Enzymes and buffers from di... - last post by jerryshelly1

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 1,876 topics
  • 5,252 replies
Problem using evagreen (eva... - last post by memari

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 569 topics
  • 1,237 replies
Trouble with shRNA transfec... - last post by Chtinico

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 1,900 topics
  • 3,655 replies
Vesicular Monoamine Transpo... - last post by Heli

Protein Expression and Purification

  • 632 topics
  • 1,566 replies
Membrane protein - last post by manavid

SDS-PAGE and Western Blotting

  • 991 topics
  • 3,339 replies
Suggestions - Nice Western... - last post by Iceage

Immunology

Discussions on antibody, immunohistochemistry and more...

  • 1,510 topics
  • 2,441 replies
Bone marrow chimera mice di... - last post by bob1

Microbiology

Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 853 topics
  • 2,313 replies
Difference between wild-typ... - last post by Mindologist

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 150 topics
  • 255 replies
Genomic DNA and plasmid DNA... - last post by phage434

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 551 topics
  • 1,415 replies
what is the effectors of MSP? - last post by pcrman

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 263 topics
  • 865 replies
Low DNA concentration for s... - last post by pito

Macroarray and Microarray

Topics related to different array techniques

  • 104 topics
  • 184 replies
How do you compare expressi... - last post by mdfenko

Neuroscience

Questions related to neuroscience and other neuro-related fields

  • 170 topics
  • 240 replies
brain slices - last post by notjustalabtech

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 150 topics
  • 359 replies
how to mark and identify mi... - last post by notjustalabtech

Botany and Plant Biology

Topics specific to plants.

  • 86 topics
  • 190 replies
Poor agrobacterium growth f... - last post by hall_b

Pharmacology and Pharmaceutics

  • 68 topics
  • 124 replies
How to calculate drug amoun... - last post by rhombus

Histology and Pathology

  • 94 topics
  • 142 replies
Fixed frozen mouse brain se... - last post by notjustalabtech

Hematology

  • 24 topics
  • 57 replies
about colony assay - last post by atashia

Chemistry

  • 43 topics
  • 112 replies
calculation of salts in pot... - last post by mdfenko



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