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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,980 topics
  • 6,186 replies
Pan-Laminin - last post by BioS

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 657 topics
  • 1,206 replies
graphing/statistical software - last post by jennifer_ph


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 526 topics
  • 1,125 replies
Salt in medium - last post by Zagami Francesco

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 143 topics
  • 362 replies
Yre project - last post by Radu Coman

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,247 topics
  • 3,874 replies
Should Lineage marker cockt... - last post by Creative Biolabs

Tissue and Cell Culture

  • 1,829 topics
  • 4,017 replies
Decrease of total cells in... - last post by Angrysh

Stem Cell

  • 139 topics
  • 211 replies
Cryopreserving human ES dir... - last post by neuropath

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 432 topics
  • 863 replies
negative control readings - last post by Angeline

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,284 topics
  • 13,188 replies
Weak band in negative contr... - last post by OldCloner

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,984 topics
  • 6,523 replies
Plasmid dna size - last post by OldCloner

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,360 topics
  • 6,429 replies
what may have gone wrong? - last post by yobou

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 665 topics
  • 1,387 replies
All the same family miRNAs... - last post by Ely Bio

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,131 topics
  • 4,176 replies
Percentage of Outer Membran... - last post by hops proteon

Protein Expression and Purification

  • 826 topics
  • 2,045 replies
GLUT1 and GLUT4 expression... - last post by harry348

SDS-PAGE and Western Blotting

  • 1,206 topics
  • 4,051 replies
Severe or entire Blur forma... - last post by aimikins


Discussions on antibody, immunohistochemistry and more...

  • 1,681 topics
  • 2,772 replies
Berthold Technologies GmbH... - last post by Jurigo


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,112 topics
  • 3,232 replies
Can M. tuberculosis grow on... - last post by OldCloner

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 227 topics
  • 348 replies
Population Genetics (interp... - last post by notsoscientist

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 623 topics
  • 1,667 replies
Methylight PCR issues - last post by RKA

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 349 topics
  • 989 replies
Variable ChiP-qPCR even in... - last post by Sandeep Gupta

Macroarray and Microarray

Topics related to different array techniques

  • 129 topics
  • 234 replies
online microarray data - last post by LizRepositive


Questions related to neuroscience and other neuro-related fields

  • 194 topics
  • 269 replies
How do photoreceptors disti... - last post by Trof

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 185 topics
  • 419 replies
Is it possible to cause mor... - last post by molbiolnew

Botany and Plant Biology

Topics specific to plants.

  • 117 topics
  • 281 replies
In vitro culture saffron - last post by mdfenko

Pharmacology and Pharmaceutics

  • 84 topics
  • 164 replies
Crosslinking with glutarald... - last post by labtastic

Histology and Pathology

  • 166 topics
  • 210 replies
Is it possible to use OCT c... - last post by harry348


  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof


  • 65 topics
  • 168 replies
Ethyl alcohol dehydration - last post by Aida shaer

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