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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

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Unusual Issues with Confoca... - last post by AAV

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 680 topics
  • 1,230 replies
How to retrieve miRNA seque... - last post by bob1


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 531 topics
  • 1,135 replies
Peroxidase assay - last post by Zagami Francesco

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 148 topics
  • 366 replies
Design Format - last post by Stephbaker

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,263 topics
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How to calculate cell viabi... - last post by mdfenko

Tissue and Cell Culture

  • 1,845 topics
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what is mean by Mock cell? - last post by pito

Stem Cell

  • 139 topics
  • 212 replies
Differentiation of human co... - last post by Stephbaker

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 436 topics
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Isotype control vs. stained... - last post by Thomson

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

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What applications can you t... - last post by doxorubicin

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

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Trouble transforming pET28a... - last post by OldCloner

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,381 topics
  • 6,479 replies
viral RNA extraction kit - last post by SF_HK

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 671 topics
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Help in gene editing - last post by tesla2020

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,137 topics
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Standard HCL protein hydrol... - last post by mdfenko

Protein Expression and Purification

  • 833 topics
  • 2,060 replies
Virus vs protein purification - last post by mdfenko

SDS-PAGE and Western Blotting

  • 1,217 topics
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How Best to Clean the Sonic... - last post by Cascadehealthcare


Discussions on antibody, immunohistochemistry and more...

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How do Ab/Ag complexes trig... - last post by electros


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,134 topics
  • 3,286 replies
Bacteria Identification - last post by Phil Geis

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 230 topics
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molecular biology - last post by mdfenko

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 624 topics
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"Binomial" and its... - last post by MindlessJPEG

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 352 topics
  • 991 replies
Sample storage during ChIP... - last post by Artemis2007

Macroarray and Microarray

Topics related to different array techniques

  • 132 topics
  • 237 replies
understanding array - last post by mdfenko


Questions related to neuroscience and other neuro-related fields

  • 196 topics
  • 272 replies
Immunofluorescence quantifi... - last post by Inmost sun

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 186 topics
  • 419 replies
Locating the Mouse AV Node - last post by Gray.MA

Botany and Plant Biology

Topics specific to plants.

  • 122 topics
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Germination test - last post by bioxesus

Pharmacology and Pharmaceutics

  • 86 topics
  • 165 replies
Calcium Imaging Calibration - last post by CristianaCris

Histology and Pathology

  • 173 topics
  • 217 replies
Antigen Retrieval - last post by SAT142


  • 33 topics
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Freezing PBMCs that had bee... - last post by Trof


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Managing Gibbs free energy - last post by bob1

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