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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,806 topics
  • 5,817 replies
Dilution calculation - last post by fred_33

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 602 topics
  • 1,136 replies
how to draw a correlation p... - last post by phdgirl


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 478 topics
  • 997 replies
RNA extraction and DNase tr... - last post by phage434

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 123 topics
  • 323 replies
Research topic related to j... - last post by Allan Chan

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,180 topics
  • 3,798 replies
Can activation of EGFR down... - last post by CPRES

Tissue and Cell Culture

  • 1,681 topics
  • 3,794 replies
Black dots-ghost cells or c... - last post by Kamil

Stem Cell

  • 136 topics
  • 204 replies
Problem in mouse embryonic... - last post by dhanur

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 398 topics
  • 837 replies
Ab titration - last post by CPRES

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,086 topics
  • 12,852 replies
Protein expression very low... - last post by Lashwar

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,875 topics
  • 6,220 replies
No colonies on plate after... - last post by CPRES

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,195 topics
  • 6,089 replies
A problem with PCR array - last post by dnasequencer

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 629 topics
  • 1,331 replies
Discovering protein–lncRNA... - last post by CPRES

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,043 topics
  • 3,985 replies
Sirt1 molecular weight - last post by Bi0l0gist

Protein Expression and Purification

  • 741 topics
  • 1,826 replies
Question about MBP purifica... - last post by labtastic

SDS-PAGE and Western Blotting

  • 1,118 topics
  • 3,787 replies
Is it possible to "over... - last post by mdfenko


Discussions on antibody, immunohistochemistry and more...

  • 1,627 topics
  • 2,670 replies
Where do you purchase T cel... - last post by CPRES


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,019 topics
  • 2,971 replies
spore production of lactoba... - last post by CPRES

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 198 topics
  • 325 replies
Dedicated microarray analysis - last post by CPRES

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 602 topics
  • 1,607 replies
housekeeping/reference gene... - last post by Lj Lee

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 325 topics
  • 967 replies
A close NGS system. What do... - last post by Curtis

Macroarray and Microarray

Topics related to different array techniques

  • 124 topics
  • 225 replies
Step-by-step microarray pro... - last post by CPRES


Questions related to neuroscience and other neuro-related fields

  • 187 topics
  • 264 replies
comercial BBB kit - last post by CPRES

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 179 topics
  • 412 replies
female BALB/c mice eating a... - last post by Fungus_Dreams

Botany and Plant Biology

Topics specific to plants.

  • 108 topics
  • 255 replies
cell division rates - last post by CPRES

Pharmacology and Pharmaceutics

  • 77 topics
  • 143 replies
Ebola Virus - last post by Einfolge

Histology and Pathology

  • 133 topics
  • 181 replies
First Nissl stain, then Gol... - last post by Marian3001


  • 32 topics
  • 82 replies
how to make Percoll solutio... - last post by mdfenko


  • 57 topics
  • 152 replies
5 M NaCl stock - last post by Ahyi

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