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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,840 topics
  • 5,908 replies
Percipitate while pH settin... - last post by El Crazy Xabi

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 612 topics
  • 1,144 replies
Different calculation of Br... - last post by Marvin


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 486 topics
  • 1,021 replies
Effect of reaction volume o... - last post by mdfenko

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 133 topics
  • 339 replies
comprehensive search for a... - last post by phdgirl

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,199 topics
  • 3,823 replies
Distribution of cell recept... - last post by Jon Moulton

Tissue and Cell Culture

  • 1,724 topics
  • 3,865 replies
Contamination in 96 well cl... - last post by Artemis2007

Stem Cell

  • 136 topics
  • 208 replies
Stem Cell Culture Theory - last post by biomart

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 409 topics
  • 846 replies
Which markers to distinguis... - last post by EvelynMDC

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,126 topics
  • 12,916 replies
cDNA library construction - last post by Leonor Bedoya

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,910 topics
  • 6,331 replies
Problems sequencing ligatio... - last post by phage434

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,234 topics
  • 6,191 replies
Standardizing cDNA concentr... - last post by phage434

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 639 topics
  • 1,347 replies
cell viability effect and i... - last post by bongiwoman

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,062 topics
  • 4,021 replies
Proteomics on samples store... - last post by DaisyT

Protein Expression and Purification

  • 757 topics
  • 1,867 replies
after protein purification - last post by labtastic

SDS-PAGE and Western Blotting

  • 1,137 topics
  • 3,860 replies
Troubleshooting for BN PAGE - last post by mdfenko


Discussions on antibody, immunohistochemistry and more...

  • 1,644 topics
  • 2,692 replies
ELISA with murine serum - last post by CandyTon


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,037 topics
  • 3,011 replies
16S Ribosomal Sequencing - last post by Burak Kaan Yasdı

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 207 topics
  • 331 replies
SNP arrays (23andme and oth... - last post by Trof

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 606 topics
  • 1,616 replies
MSP_Primer dimers? - last post by elenagardini

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 331 topics
  • 971 replies
Flash freezing ChIP samples... - last post by Dr_Rob

Macroarray and Microarray

Topics related to different array techniques

  • 125 topics
  • 226 replies
online microarray data - last post by Srilatha Dharmavaram


Questions related to neuroscience and other neuro-related fields

  • 189 topics
  • 265 replies
Measuring IPSPs in a pacema... - last post by Sakumi

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 182 topics
  • 416 replies
Routine medical tests on mi... - last post by pito

Botany and Plant Biology

Topics specific to plants.

  • 110 topics
  • 263 replies
What plant species are you... - last post by Labmate0910

Pharmacology and Pharmaceutics

  • 80 topics
  • 150 replies
Preferred assay for measuri... - last post by DRT

Histology and Pathology

  • 139 topics
  • 188 replies
LUNA Staining Protocol to d... - last post by Empath


  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof


  • 57 topics
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5 M NaCl stock - last post by Ahyi

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