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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,749 topics
  • 5,668 replies
problem with calculation - last post by bob1

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 583 topics
  • 1,104 replies
Question about MOTHUR conce... - last post by Procyon


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 465 topics
  • 976 replies
Pectinase Enzyme Assay - last post by mdfenko

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 118 topics
  • 316 replies
new trends in rice science - last post by pito

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,161 topics
  • 3,757 replies
MTT assay without positive... - last post by vinclam

Tissue and Cell Culture

  • 1,620 topics
  • 3,675 replies
Same Class II hood for diff... - last post by floyd78

Stem Cell

  • 134 topics
  • 197 replies
how to transport stem cells... - last post by RLAD

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 385 topics
  • 807 replies
Biotin-streptavidin signal... - last post by JGBarin

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,003 topics
  • 12,622 replies
Changing the vector - last post by bob1

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,818 topics
  • 6,052 replies
Mutagenesis PCR - last post by Trof

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,144 topics
  • 5,984 replies
Consistancy issue with PCR... - last post by Trof

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 619 topics
  • 1,319 replies
prediction of microRNA targ... - last post by pcrman

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,008 topics
  • 3,910 replies
problems with 1D SDS PAGE - last post by mdfenko

Protein Expression and Purification

  • 719 topics
  • 1,786 replies
CO- IP for nuclear protein - last post by bob1

SDS-PAGE and Western Blotting

  • 1,083 topics
  • 3,686 replies
2x laemmli buffer - last post by bob1


Discussions on antibody, immunohistochemistry and more...

  • 1,606 topics
  • 2,625 replies
Preparation of antibody column - last post by mdfenko


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 989 topics
  • 2,899 replies
Problems with M13 phage - last post by axb085

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 190 topics
  • 317 replies
Mutation screening - last post by bob1

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 596 topics
  • 1,596 replies
No sequencing  of DNA methy... - last post by phage434

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 315 topics
  • 945 replies
Chromatin Isolation Trouble... - last post by vp1987

Macroarray and Microarray

Topics related to different array techniques

  • 120 topics
  • 219 replies
Array Data for tumours (lun... - last post by Ahyi


Questions related to neuroscience and other neuro-related fields

  • 182 topics
  • 257 replies
Mice LTP is not working - last post by Inmost sun

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 175 topics
  • 406 replies
orthotopic mouse model of U... - last post by Fungus_Dreams

Botany and Plant Biology

Topics specific to plants.

  • 102 topics
  • 242 replies
Why would you use DNase as... - last post by sara_molecular

Pharmacology and Pharmaceutics

  • 75 topics
  • 143 replies
What is the best statisical... - last post by DRT

Histology and Pathology

  • 123 topics
  • 174 replies
B-gal staining troubleshoot... - last post by Irina_Vavi


  • 31 topics
  • 76 replies
RNA isolation from whole blood - last post by Trof


  • 57 topics
  • 149 replies
Acid-Base Buffer Question - last post by bob1

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