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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,930 topics
  • 6,108 replies
Dye does not separe in acry... - last post by piemmea

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 653 topics
  • 1,195 replies
Pathway analysis - last post by bob1


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 518 topics
  • 1,087 replies
Rate of blood hydrolization - last post by Batbilegt

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 139 topics
  • 357 replies
A contagious man infecting... - last post by bob1

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,240 topics
  • 3,867 replies
Freezing cell lysate for la... - last post by matheusmlot

Tissue and Cell Culture

  • 1,802 topics
  • 3,973 replies
Increasing lipofection effi... - last post by bob1

Stem Cell

  • 139 topics
  • 211 replies
Cryopreserving human ES dir... - last post by neuropath

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 423 topics
  • 860 replies
No-wash fixation/permeabili... - last post by bob1

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,248 topics
  • 13,121 replies
Qubit or DEnovix QFX??? - last post by Ariel Ignacio Suarez

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,966 topics
  • 6,488 replies
Using pB2GW7,0 - last post by Chomolungma

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,327 topics
  • 6,375 replies
PCR Product for External qP... - last post by delorend

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 659 topics
  • 1,371 replies
Where can I find 100-200 un... - last post by Bio21

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,108 topics
  • 4,135 replies
Mass Spectrometry help needed - last post by rick112

Protein Expression and Purification

  • 809 topics
  • 2,004 replies
Fab antibody fragment purif... - last post by Missle

SDS-PAGE and Western Blotting

  • 1,192 topics
  • 4,027 replies
Automated Western blot systems - last post by lisam


Discussions on antibody, immunohistochemistry and more...

  • 1,673 topics
  • 2,759 replies
DAB & DAPI together for... - last post by miza


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,097 topics
  • 3,191 replies
How to adjust the pH of a b... - last post by El Crazy Xabi

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 219 topics
  • 345 replies
CRISPR-Cas9 / insert a gene... - last post by bob1

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 620 topics
  • 1,663 replies
Tips for MSP ... - last post by science64

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 347 topics
  • 989 replies
Radiactive PCR for ChIP qua... - last post by autophagator

Macroarray and Microarray

Topics related to different array techniques

  • 129 topics
  • 234 replies
online microarray data - last post by LizRepositive


Questions related to neuroscience and other neuro-related fields

  • 192 topics
  • 267 replies
What is EPSP slope ? - last post by Inmost sun

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 184 topics
  • 418 replies
Free software to analyze IV... - last post by neuropath

Botany and Plant Biology

Topics specific to plants.

  • 116 topics
  • 280 replies
What does "R.H. 1619... - last post by nbic

Pharmacology and Pharmaceutics

  • 83 topics
  • 163 replies
Calculating whole body toxi... - last post by bob1

Histology and Pathology

  • 157 topics
  • 202 replies
IHC Protein Competition, Pr... - last post by bob1


  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof


  • 60 topics
  • 159 replies
Determination of Phenolic c... - last post by Dima

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