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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,772 topics
  • 5,722 replies
Final % DMSO Concentration - last post by Fu Shun Wong

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 590 topics
  • 1,120 replies
If there is two different s... - last post by bob1


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 472 topics
  • 983 replies
Protein expression in E. co... - last post by Doc

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 119 topics
  • 316 replies
Paper-roll Chromatography/E... - last post by 27thcentury

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,170 topics
  • 3,769 replies
Does Opti-mem contain serum - last post by Tabaluga

Tissue and Cell Culture

  • 1,648 topics
  • 3,729 replies
Recurrent yeast contamination - last post by SusieQ

Stem Cell

  • 134 topics
  • 200 replies
Totipotent Stem Cell From M... - last post by caters

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 390 topics
  • 819 replies
cell viability with PI stai... - last post by Tabaluga

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,037 topics
  • 12,755 replies
Can't detect GAPDH (wes... - last post by bob1

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,835 topics
  • 6,120 replies
ratios insert/vector - last post by phage434

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,164 topics
  • 6,030 replies
normalization of qPCR results - last post by FCS scholar

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 623 topics
  • 1,321 replies
How to retrieve precursor m... - last post by adamos1945

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,029 topics
  • 3,942 replies
MAPK/MEK Inhibitor stored i... - last post by matthew.summers

Protein Expression and Purification

  • 729 topics
  • 1,804 replies
EGFP expression - last post by labtastic

SDS-PAGE and Western Blotting

  • 1,098 topics
  • 3,729 replies
Protein bands visible on un... - last post by terpenie


Discussions on antibody, immunohistochemistry and more...

  • 1,616 topics
  • 2,637 replies
concentration of samples wi... - last post by Tielli


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,006 topics
  • 2,935 replies
Selectively arresting a sub... - last post by yeastresearcher22

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 196 topics
  • 324 replies
Can we identify genetic mar... - last post by Raywalker09

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 600 topics
  • 1,607 replies
MNase assay - how to quanti... - last post by phage434

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 320 topics
  • 952 replies
No signal in qPCR from ChIP... - last post by ndswxy

Macroarray and Microarray

Topics related to different array techniques

  • 120 topics
  • 219 replies
Array Data for tumours (lun... - last post by Ahyi


Questions related to neuroscience and other neuro-related fields

  • 183 topics
  • 258 replies
What is EPSP slope ? - last post by Cainosito Cainositas

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 179 topics
  • 407 replies
female BALB/c mice eating a... - last post by bob1

Botany and Plant Biology

Topics specific to plants.

  • 104 topics
  • 245 replies
problems on the media for o... - last post by tamarine

Pharmacology and Pharmaceutics

  • 75 topics
  • 143 replies
What is the best statisical... - last post by DRT

Histology and Pathology

  • 127 topics
  • 177 replies
Tri-Crome stain always purp... - last post by Empty_H


  • 32 topics
  • 82 replies
how to make Percoll solutio... - last post by mdfenko


  • 57 topics
  • 151 replies
Acid-Base Buffer Question - last post by bob1

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