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Protocols and Techniques Forums


Subforums

General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,498 topics
  • 4,905 replies
Plasmid stability in Ecoli... - last post by leelee

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 494 topics
  • 915 replies
[Video] Using NCBI for RT-P... - last post by bioforum

Biochemistry

Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 401 topics
  • 853 replies
Citrate synthase standard f... - last post by mdfenko

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 101 topics
  • 263 replies
Launching a new Influenza F... - last post by Mohamed samir

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,039 topics
  • 3,572 replies
"Dear" GPCR - last post by Tom M

Tissue and Cell Culture

  • 1,361 topics
  • 3,119 replies
How to culture HK-2 cells - last post by leelee

Stem Cell

  • 114 topics
  • 182 replies
problems in culturing mice... - last post by mesenchymal stem cell

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 329 topics
  • 721 replies
PI stain for cell viability... - last post by rpjkmust916

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 5,668 topics
  • 11,598 replies
Weak promoter activity in p... - last post by pcrman

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,540 topics
  • 5,108 replies
Too many negative clones us... - last post by srpres

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 1,851 topics
  • 5,190 replies
real time PCR trouble - last post by lula

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 566 topics
  • 1,231 replies
miRNA sequencing from FFPE... - last post by pcrman

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 1,890 topics
  • 3,642 replies
Biotinylation - last post by Curtis

Protein Expression and Purification

  • 627 topics
  • 1,552 replies
tag for protein purification - last post by labtastic

SDS-PAGE and Western Blotting

  • 974 topics
  • 3,269 replies
Native-PAGE, help! - last post by le_duy211

Immunology

Discussions on antibody, immunohistochemistry and more...

  • 1,506 topics
  • 2,434 replies
Take 5 Minute Ab/ELISA Surv... - last post by marycanady

Microbiology

Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 847 topics
  • 2,298 replies
Deactivation of bacteria fo... - last post by El Crazy Xabi

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 149 topics
  • 249 replies
Design construct for promot... - last post by pcrman

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 549 topics
  • 1,411 replies
experiments related to epig... - last post by DeniseB

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 258 topics
  • 858 replies
ChIP, Sonication and SDS - last post by j47027

Macroarray and Microarray

Topics related to different array techniques

  • 101 topics
  • 180 replies
QC saga continues.... from... - last post by mdfenko

Neuroscience

Questions related to neuroscience and other neuro-related fields

  • 168 topics
  • 236 replies
Lysis buffer for white matter - last post by Curtis

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 150 topics
  • 358 replies
CO2 to sedate animals - last post by bob1

Botany and Plant Biology

Topics specific to plants.

  • 86 topics
  • 190 replies
Poor agrobacterium growth f... - last post by hall_b

Pharmacology and Pharmaceutics

  • 66 topics
  • 122 replies
Stimulate receptor constitu... - last post by Annasae

Histology and Pathology

  • 94 topics
  • 141 replies
restain secondary antibody - last post by wdyeo

Hematology

  • 24 topics
  • 57 replies
about colony assay - last post by atashia

Chemistry

  • 42 topics
  • 107 replies
Possible substitutions of H... - last post by Annasae



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