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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,916 topics
  • 6,075 replies
Standard curve using ImageJ - last post by CristianaCris

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 647 topics
  • 1,188 replies
Can someone explain in laym... - last post by bob1


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 510 topics
  • 1,063 replies
Solvents for Mass Spec invo... - last post by biochem707

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 139 topics
  • 357 replies
A contagious man infecting... - last post by bob1

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,234 topics
  • 3,864 replies
ENZYME BASED ASSAYS TO CHEC... - last post by Chomolungma

Tissue and Cell Culture

  • 1,791 topics
  • 3,951 replies
Splitting cells without cen... - last post by Roboclaw

Stem Cell

  • 138 topics
  • 211 replies
Stem Cell Donation - last post by Chloe Mica

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 419 topics
  • 858 replies
How to clean up cell fragme... - last post by Andrea Fortina

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,228 topics
  • 13,089 replies
Good diluents for RNA - last post by swannnyy

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,961 topics
  • 6,476 replies
Unexpected band in BL21 (DE... - last post by labtastic

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,316 topics
  • 6,364 replies
Strange Taqman probe negati... - last post by Trof

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 658 topics
  • 1,371 replies
negative control miRNA - last post by bob1

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,105 topics
  • 4,123 replies
Mass Spectrometry help needed - last post by biochem06

Protein Expression and Purification

  • 800 topics
  • 1,986 replies
HSP70 contamination after N... - last post by labtastic

SDS-PAGE and Western Blotting

  • 1,189 topics
  • 4,019 replies
2D Blots problems - last post by biochem06


Discussions on antibody, immunohistochemistry and more...

  • 1,664 topics
  • 2,747 replies
Effect of collagenase on re... - last post by Sarah Whelan


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,087 topics
  • 3,178 replies
Disinfectant Efficacy Testing - last post by Sana4025

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 217 topics
  • 342 replies
Map Viewer for several genes - last post by Trof

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 618 topics
  • 1,661 replies
Baseline Noise on Bisulfite... - last post by MaryC

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 345 topics
  • 989 replies
Miseq Minimum number of RNA... - last post by Chris22

Macroarray and Microarray

Topics related to different array techniques

  • 128 topics
  • 232 replies
Microarray resources - last post by mdfenko


Questions related to neuroscience and other neuro-related fields

  • 192 topics
  • 267 replies
What is EPSP slope ? - last post by Inmost sun

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 183 topics
  • 416 replies
E13.5 mouse embryo staining... - last post by Ambinlab

Botany and Plant Biology

Topics specific to plants.

  • 116 topics
  • 280 replies
What does "R.H. 1619... - last post by nbic

Pharmacology and Pharmaceutics

  • 82 topics
  • 156 replies
Preparation of antibiotic disc - last post by mdfenko

Histology and Pathology

  • 154 topics
  • 201 replies
LR white resin and H&E... - last post by snd


  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof


  • 59 topics
  • 158 replies
EDTA precipitates out of ED... - last post by mdfenko

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