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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,974 topics
  • 6,173 replies
Papain activity calculation - last post by bob1

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 657 topics
  • 1,204 replies
Reading Alignments (overlap... - last post by Manaka


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 526 topics
  • 1,119 replies
What are the side-effects o... - last post by aimikins

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 143 topics
  • 362 replies
Yre project - last post by Radu Coman

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,246 topics
  • 3,873 replies
Should Lineage marker cockt... - last post by rv1234

Tissue and Cell Culture

  • 1,823 topics
  • 4,011 replies
Chloramphenicol or Streptom... - last post by Kristaliukas

Stem Cell

  • 139 topics
  • 211 replies
Cryopreserving human ES dir... - last post by neuropath

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 429 topics
  • 863 replies
flow cytometry of intracell... - last post by chebak meryem

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,277 topics
  • 13,167 replies
restriction enzyme pcr cloning - last post by bob1

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,979 topics
  • 6,509 replies
Plasmid distributors for Lu... - last post by Helder_90

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,354 topics
  • 6,424 replies
Optimization of real time-P... - last post by bob1

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 664 topics
  • 1,387 replies
We need a way to see microR... - last post by Fredreload

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,127 topics
  • 4,172 replies
Reduction of Foam formation - last post by Siniya

Protein Expression and Purification

  • 825 topics
  • 2,043 replies
Time period of sodiun laury... - last post by Siniya

SDS-PAGE and Western Blotting

  • 1,206 topics
  • 4,051 replies
Severe or entire Blur forma... - last post by aimikins


Discussions on antibody, immunohistochemistry and more...

  • 1,678 topics
  • 2,772 replies
ELISA and Sugar/saccharides - last post by mdfenko


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,107 topics
  • 3,205 replies
Antibiotics - last post by rihnavy

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 226 topics
  • 348 replies
Pyura DNA extraction - last post by El Crazy Xabi

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 622 topics
  • 1,667 replies
Bisulfite conversion, PCR e... - last post by mavpouck2

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 349 topics
  • 989 replies
Variable ChiP-qPCR even in... - last post by Sandeep Gupta

Macroarray and Microarray

Topics related to different array techniques

  • 129 topics
  • 234 replies
online microarray data - last post by LizRepositive


Questions related to neuroscience and other neuro-related fields

  • 194 topics
  • 269 replies
How do photoreceptors disti... - last post by Trof

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 185 topics
  • 419 replies
Is it possible to cause mor... - last post by molbiolnew

Botany and Plant Biology

Topics specific to plants.

  • 117 topics
  • 281 replies
In vitro culture saffron - last post by mdfenko

Pharmacology and Pharmaceutics

  • 84 topics
  • 164 replies
Crosslinking with glutarald... - last post by labtastic

Histology and Pathology

  • 161 topics
  • 206 replies
Gram stain compatibility wi... - last post by stamatina_tr


  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof


  • 63 topics
  • 167 replies
Dipyridyl with Trypticase s... - last post by Siniya

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