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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,688 topics
  • 5,502 replies
Ethidium bromide spill conc... - last post by nymets2013ws

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 570 topics
  • 1,074 replies
Complete CDS of suid herpes... - last post by phage434


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 450 topics
  • 927 replies
hydroxy-fatty acids and gas... - last post by mdfenko

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 114 topics
  • 300 replies
HPLC detection of 5-methylc... - last post by phage434

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,141 topics
  • 3,731 replies
Immunostaing - last post by Tabaluga

Tissue and Cell Culture

  • 1,565 topics
  • 3,553 replies
Adapting a Drosophila cell... - last post by Biologystudent

Stem Cell

  • 130 topics
  • 193 replies
mouse embryonic stem cell c... - last post by Elisa90

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 378 topics
  • 788 replies
Mean of fluorescence in FlowJo - last post by Judith_NM

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 5,930 topics
  • 12,378 replies
lyophilized protein dilution - last post by harry348

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,759 topics
  • 5,805 replies
Cloning worked, but the exp... - last post by Biologystudent

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,085 topics
  • 5,825 replies
Nanodrop vs Picogreen to qu... - last post by detriar

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 609 topics
  • 1,309 replies
Experience with mirVana™ mi... - last post by Trys

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 1,986 topics
  • 3,862 replies
Heat the sample during nitr... - last post by Nicolai

Protein Expression and Purification

  • 699 topics
  • 1,743 replies
Transient transfection usin... - last post by bob1

SDS-PAGE and Western Blotting

  • 1,061 topics
  • 3,587 replies
My beta actin bands are cha... - last post by sialic acid


Discussions on antibody, immunohistochemistry and more...

  • 1,587 topics
  • 2,593 replies
How to check standard curve... - last post by tkf


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 939 topics
  • 2,680 replies
Xanthomonas alfalfae subsp... - last post by mikyoung23

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 183 topics
  • 306 replies
FISH - Cot-1 DNA - last post by biofingers

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 584 topics
  • 1,542 replies
Nested BSP Primer Design - last post by phage434

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 304 topics
  • 935 replies
ChIP Buffers - last post by jerryshelly1

Macroarray and Microarray

Topics related to different array techniques

  • 115 topics
  • 214 replies
How to test if probes are u... - last post by Inbox


Questions related to neuroscience and other neuro-related fields

  • 178 topics
  • 250 replies
Membrane sensitive dyes: Th... - last post by Wurmbox

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 174 topics
  • 402 replies
Harvesting tumours for re-t... - last post by bob1

Botany and Plant Biology

Topics specific to plants.

  • 99 topics
  • 230 replies
What biochemicals are invol... - last post by mogokll

Pharmacology and Pharmaceutics

  • 75 topics
  • 143 replies
What is the best statisical... - last post by DRT

Histology and Pathology

  • 115 topics
  • 158 replies
Methanol/acetic acid fixation - last post by bob1


  • 29 topics
  • 63 replies
Screwed up blood extraction... - last post by Relaxosome


  • 55 topics
  • 130 replies
Does autoclaving salts of m... - last post by p.phadtare

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