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Protocols and Techniques Forums


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General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,753 topics
  • 5,675 replies
Need some Fluorescent Micro... - last post by wcsoccerfan

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 583 topics
  • 1,105 replies
Affymatrix Microarray data - last post by CD Genomics

Biochemistry

Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 468 topics
  • 981 replies
Glycolysis oxidation states - last post by bob1

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 118 topics
  • 316 replies
new trends in rice science - last post by pito

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,163 topics
  • 3,758 replies
Karyotyping, Giemsa Staining - last post by cell slave

Tissue and Cell Culture

  • 1,622 topics
  • 3,683 replies
DMEM medium with HEPES and... - last post by sara.r

Stem Cell

  • 134 topics
  • 200 replies
Totipotent Stem Cell From M... - last post by caters

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 385 topics
  • 807 replies
Biotin-streptavidin signal... - last post by JGBarin

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,009 topics
  • 12,647 replies
Superdupercoiling: what aff... - last post by mdfenko

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,823 topics
  • 6,081 replies
Cloning of 9mer nucleotide... - last post by dpo

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,151 topics
  • 5,999 replies
RT-PCR primer design and ps... - last post by sara.r

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 619 topics
  • 1,319 replies
prediction of microRNA targ... - last post by pcrman

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,009 topics
  • 3,912 replies
Choosing MWCO For Dialysis... - last post by Artemis2007

Protein Expression and Purification

  • 720 topics
  • 1,792 replies
i am trying to get rid of u... - last post by labtastic

SDS-PAGE and Western Blotting

  • 1,084 topics
  • 3,693 replies
Native PAGE problem - not e... - last post by Ya yesh

Immunology

Discussions on antibody, immunohistochemistry and more...

  • 1,607 topics
  • 2,626 replies
lymphocytes-myeloma fusions - last post by XePtI

Microbiology

Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 992 topics
  • 2,908 replies
Protocol for Bile Salt Hydr... - last post by Celz

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 192 topics
  • 321 replies
Transgenic Research - last post by Dianna1234

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 597 topics
  • 1,596 replies
Finding methylation in the... - last post by saravana devi

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 316 topics
  • 947 replies
Mock IgG versus No Antibody... - last post by yy99875

Macroarray and Microarray

Topics related to different array techniques

  • 120 topics
  • 219 replies
Array Data for tumours (lun... - last post by Ahyi

Neuroscience

Questions related to neuroscience and other neuro-related fields

  • 182 topics
  • 257 replies
Mice LTP is not working - last post by Inmost sun

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 175 topics
  • 406 replies
orthotopic mouse model of U... - last post by Fungus_Dreams

Botany and Plant Biology

Topics specific to plants.

  • 102 topics
  • 242 replies
Why would you use DNase as... - last post by sara_molecular

Pharmacology and Pharmaceutics

  • 75 topics
  • 143 replies
What is the best statisical... - last post by DRT

Histology and Pathology

  • 123 topics
  • 174 replies
B-gal staining troubleshoot... - last post by Irina_Vavi

Hematology

  • 32 topics
  • 78 replies
Unusual Buffy Coat - (I Think) - last post by djvan

Chemistry

  • 57 topics
  • 149 replies
Acid-Base Buffer Question - last post by bob1



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