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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,765 topics
  • 5,696 replies
software for plasmid drawing - last post by branthackett

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 585 topics
  • 1,115 replies
[Software] Drawing arrows a... - last post by branthackett


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 470 topics
  • 982 replies
The project title is ôStudi... - last post by bob1

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 118 topics
  • 316 replies
new trends in rice science - last post by pito

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,166 topics
  • 3,765 replies
can anyone tell me what kin... - last post by bob1

Tissue and Cell Culture

  • 1,633 topics
  • 3,698 replies
problem with cell synchroni... - last post by bob1

Stem Cell

  • 134 topics
  • 200 replies
Totipotent Stem Cell From M... - last post by caters

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 387 topics
  • 815 replies
Viability dye 7-AAD - last post by Tabaluga

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,022 topics
  • 12,683 replies
Separaing double stranded dna - last post by phage434

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,828 topics
  • 6,095 replies
Is important the direction... - last post by esmaeilzadeh

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,158 topics
  • 6,010 replies
Act1 mRNA decay is not linear - last post by aznbabie

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 620 topics
  • 1,320 replies
lincRNA-p21 sequence - last post by Martin Faber

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,015 topics
  • 3,924 replies
protein biotinylation - last post by claire84

Protein Expression and Purification

  • 722 topics
  • 1,794 replies
Purification of metallothio... - last post by labtastic

SDS-PAGE and Western Blotting

  • 1,088 topics
  • 3,707 replies
Stripping problem - last post by jaya2020


Discussions on antibody, immunohistochemistry and more...

  • 1,610 topics
  • 2,627 replies
Hybridoma - last post by Deborah


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 997 topics
  • 2,913 replies
periplasmic fraction extrac... - last post by vinih

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 193 topics
  • 323 replies
Ethidium bromide mutagenesis - last post by pito

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 598 topics
  • 1,604 replies
EZ DNA Methylation-Lightnin... - last post by phage434

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 318 topics
  • 952 replies
ChIP: Inconsistent reverse... - last post by shp952

Macroarray and Microarray

Topics related to different array techniques

  • 120 topics
  • 219 replies
Array Data for tumours (lun... - last post by Ahyi


Questions related to neuroscience and other neuro-related fields

  • 183 topics
  • 257 replies
Perfusion of neonatal mouse? - last post by Dbrainz

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 178 topics
  • 406 replies
CpG injection for IFNalpha... - last post by BartL

Botany and Plant Biology

Topics specific to plants.

  • 104 topics
  • 242 replies
Is methyl jasmonate equival... - last post by astroboy89

Pharmacology and Pharmaceutics

  • 75 topics
  • 143 replies
What is the best statisical... - last post by DRT

Histology and Pathology

  • 124 topics
  • 174 replies
Toluidine blue o staining i... - last post by E-biologist


  • 32 topics
  • 78 replies
Unusual Buffy Coat - (I Think) - last post by djvan


  • 57 topics
  • 151 replies
Acid-Base Buffer Question - last post by bob1

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