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Protocols and Techniques Forums


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General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,941 topics
  • 6,120 replies
lab automation advice - last post by molbio1234

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 655 topics
  • 1,198 replies
Three experiments with thre... - last post by DRT

Biochemistry

Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 522 topics
  • 1,107 replies
How to add M1,M2,M3, etc - last post by bob1

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 139 topics
  • 357 replies
A contagious man infecting... - last post by bob1

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,240 topics
  • 3,867 replies
Freezing cell lysate for la... - last post by matheusmlot

Tissue and Cell Culture

  • 1,810 topics
  • 3,985 replies
Lentivirus production issues - last post by merlav

Stem Cell

  • 139 topics
  • 211 replies
Cryopreserving human ES dir... - last post by neuropath

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 426 topics
  • 863 replies
What are the uses for posit... - last post by bob1

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,254 topics
  • 13,132 replies
Cycloheximide concentration - last post by bob1

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,971 topics
  • 6,496 replies
[Overexpression] Without up... - last post by lactamase

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,335 topics
  • 6,393 replies
How clean do I need my DNA... - last post by bob1

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 663 topics
  • 1,375 replies
We need a way to see microR... - last post by Fredreload

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,111 topics
  • 4,138 replies
What is the definition for... - last post by BIOAWY

Protein Expression and Purification

  • 810 topics
  • 2,007 replies
polb cloned in pET not expr... - last post by labtastic

SDS-PAGE and Western Blotting

  • 1,194 topics
  • 4,029 replies
Low weight protein degradat... - last post by mdfenko

Immunology

Discussions on antibody, immunohistochemistry and more...

  • 1,674 topics
  • 2,763 replies
lactose intolerance - last post by bob1

Microbiology

Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,099 topics
  • 3,191 replies
Making  Active culture and... - last post by Ahmad Ud Din

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 224 topics
  • 346 replies
Pipeline to search bacteria... - last post by Chris22

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 621 topics
  • 1,664 replies
Different efficiencies for... - last post by eyes

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 347 topics
  • 989 replies
Radiactive PCR for ChIP qua... - last post by autophagator

Macroarray and Microarray

Topics related to different array techniques

  • 129 topics
  • 234 replies
online microarray data - last post by LizRepositive

Neuroscience

Questions related to neuroscience and other neuro-related fields

  • 192 topics
  • 267 replies
What is EPSP slope ? - last post by Inmost sun

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 184 topics
  • 418 replies
Free software to analyze IV... - last post by neuropath

Botany and Plant Biology

Topics specific to plants.

  • 116 topics
  • 280 replies
What does "R.H. 1619... - last post by nbic

Pharmacology and Pharmaceutics

  • 83 topics
  • 163 replies
Calculating whole body toxi... - last post by bob1

Histology and Pathology

  • 158 topics
  • 204 replies
unusual FITC background sta... - last post by biochem06

Hematology

  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof

Chemistry

  • 62 topics
  • 160 replies
solid phase extraction cort... - last post by mdfenko



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