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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,787 topics
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Daily stimulation with cyto... - last post by bob1

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 595 topics
  • 1,128 replies
Case-control study using SP... - last post by hobglobin


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 473 topics
  • 989 replies
Non-radioactive DNA-PK acti... - last post by mdfenko

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 121 topics
  • 317 replies
Are you a scientist/invento... - last post by jobscallumco

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,172 topics
  • 3,774 replies
Books for cancer biology - last post by Inmost sun

Tissue and Cell Culture

  • 1,666 topics
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Cell Sustainability during... - last post by srimadh

Stem Cell

  • 135 topics
  • 201 replies
Stem Cell Culture Theory - last post by bob1

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 392 topics
  • 823 replies
FACS Canto II not showing e... - last post by rochaff87

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,053 topics
  • 12,776 replies
Ligation and blue white scr... - last post by KhaleesiDany

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,845 topics
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Subtle change in insert siz... - last post by Hamaguchi

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,174 topics
  • 6,040 replies
bacterial RNA isolation fro... - last post by Md Jibran Alam

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 626 topics
  • 1,324 replies
use bioedit to analyze micr... - last post by adamos1945

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,035 topics
  • 3,962 replies
Urea effect on mice spleen... - last post by mdfenko

Protein Expression and Purification

  • 732 topics
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Pichia expression- His tagg... - last post by proteinz

SDS-PAGE and Western Blotting

  • 1,105 topics
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Axin1 Co-IP - last post by miST32


Discussions on antibody, immunohistochemistry and more...

  • 1,620 topics
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How to calculate LOD of elisa - last post by tkf


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

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  • 2,948 replies
Bacteria Filtration and iso... - last post by Md Jibran Alam

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 197 topics
  • 324 replies
Genome annotation - last post by Dav

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 601 topics
  • 1,607 replies
5 aza- 2 deoxy and TSA - last post by seeking_2012

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 324 topics
  • 956 replies
Amplicons detected for nega... - last post by trungbiotech

Macroarray and Microarray

Topics related to different array techniques

  • 122 topics
  • 220 replies
Microarray-Gene fold change... - last post by bob1


Questions related to neuroscience and other neuro-related fields

  • 184 topics
  • 258 replies
mouse stellate/cervicothora... - last post by k1064192

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 179 topics
  • 411 replies
female BALB/c mice eating a... - last post by VarianWrynn

Botany and Plant Biology

Topics specific to plants.

  • 106 topics
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Is it possible to stored en... - last post by hobglobin

Pharmacology and Pharmaceutics

  • 77 topics
  • 143 replies
Ebola Virus - last post by Einfolge

Histology and Pathology

  • 129 topics
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Protocole Immunohistochemis... - last post by orianedl


  • 32 topics
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how to make Percoll solutio... - last post by mdfenko


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5 M NaCl stock - last post by Ahyi

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