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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 2,022 topics
  • 6,285 replies
Thing to knows about Scienc... - last post by SCIENCE GEEK

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 678 topics
  • 1,228 replies
Tool for matching two compl... - last post by mohsamir1984


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 531 topics
  • 1,135 replies
Peroxidase assay - last post by Zagami Francesco

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 148 topics
  • 366 replies
Design Format - last post by Stephbaker

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,261 topics
  • 3,895 replies
Heart specific biomarker - last post by aqua00

Tissue and Cell Culture

  • 1,845 topics
  • 4,042 replies
what is mean by Mock cell? - last post by pito

Stem Cell

  • 139 topics
  • 212 replies
Differentiation of human co... - last post by Stephbaker

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 436 topics
  • 867 replies
Isotype control vs. stained... - last post by Thomson

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,311 topics
  • 13,211 replies
cell free DNA measurement - last post by SF_HK

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,993 topics
  • 6,571 replies
Choosing a common exon in G... - last post by Jon Moulton

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,381 topics
  • 6,479 replies
viral RNA extraction kit - last post by SF_HK

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 668 topics
  • 1,392 replies
urinary exosomal miRNA - last post by SF_HK

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,135 topics
  • 4,181 replies
how to enhance the detectio... - last post by mdfenko

Protein Expression and Purification

  • 832 topics
  • 2,059 replies
How to eliminate inherent p... - last post by mdfenko

SDS-PAGE and Western Blotting

  • 1,217 topics
  • 4,091 replies
How Best to Clean the Sonic... - last post by Cascadehealthcare


Discussions on antibody, immunohistochemistry and more...

  • 1,694 topics
  • 2,781 replies
How do Ab/Ag complexes trig... - last post by electros


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,134 topics
  • 3,286 replies
Bacteria Identification - last post by Phil Geis

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 230 topics
  • 350 replies
molecular biology - last post by mdfenko

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 624 topics
  • 1,667 replies
"Binomial" and its... - last post by MindlessJPEG

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 351 topics
  • 991 replies
NGS sequencing and capture... - last post by genetics3

Macroarray and Microarray

Topics related to different array techniques

  • 132 topics
  • 237 replies
understanding array - last post by mdfenko


Questions related to neuroscience and other neuro-related fields

  • 196 topics
  • 270 replies
What stimuli trigger hippoc... - last post by electros

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 186 topics
  • 419 replies
Locating the Mouse AV Node - last post by Gray.MA

Botany and Plant Biology

Topics specific to plants.

  • 121 topics
  • 283 replies
Chlorophyll and other pigme... - last post by AdOl

Pharmacology and Pharmaceutics

  • 86 topics
  • 165 replies
Calcium Imaging Calibration - last post by CristianaCris

Histology and Pathology

  • 173 topics
  • 217 replies
Antigen Retrieval - last post by SAT142


  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof


  • 67 topics
  • 172 replies
Managing Gibbs free energy - last post by bob1

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