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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,804 topics
  • 5,812 replies
Please help urgently - last post by CPRES

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 601 topics
  • 1,136 replies
Upcoming NGS Workshop: A Be... - last post by ecSeq


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 477 topics
  • 996 replies
CO2 and N flushing of baker... - last post by scoob00

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 123 topics
  • 323 replies
Research topic related to j... - last post by Allan Chan

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,180 topics
  • 3,796 replies
Can activation of EGFR down... - last post by Trof

Tissue and Cell Culture

  • 1,680 topics
  • 3,793 replies
3D Cell Culture Matrix made... - last post by kohlmeise

Stem Cell

  • 136 topics
  • 204 replies
Problem in mouse embryonic... - last post by dhanur

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 398 topics
  • 837 replies
Ab titration - last post by CPRES

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,083 topics
  • 12,848 replies
Mention of Transgene Insert... - last post by CPRES

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,874 topics
  • 6,215 replies
Low Yield Miniprep - last post by Trof

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,193 topics
  • 6,080 replies
PCR & Western Blot samp... - last post by Trof

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 629 topics
  • 1,331 replies
Discovering protein–lncRNA... - last post by CPRES

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,043 topics
  • 3,984 replies
Sirt1 molecular weight - last post by CPRES

Protein Expression and Purification

  • 740 topics
  • 1,823 replies
Elute fractions that have t... - last post by EvilTwin

SDS-PAGE and Western Blotting

  • 1,116 topics
  • 3,784 replies
SDS PAGE Destaining - last post by CPRES


Discussions on antibody, immunohistochemistry and more...

  • 1,627 topics
  • 2,670 replies
Where do you purchase T cel... - last post by CPRES


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,019 topics
  • 2,971 replies
spore production of lactoba... - last post by CPRES

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 198 topics
  • 325 replies
Dedicated microarray analysis - last post by CPRES

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 602 topics
  • 1,607 replies
housekeeping/reference gene... - last post by Lj Lee

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 325 topics
  • 967 replies
A close NGS system. What do... - last post by Curtis

Macroarray and Microarray

Topics related to different array techniques

  • 123 topics
  • 224 replies
DETERMINATION OF BASELINE F... - last post by Nyameba


Questions related to neuroscience and other neuro-related fields

  • 187 topics
  • 264 replies
comercial BBB kit - last post by CPRES

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 179 topics
  • 412 replies
female BALB/c mice eating a... - last post by Fungus_Dreams

Botany and Plant Biology

Topics specific to plants.

  • 107 topics
  • 252 replies
Heart Stage Embryos - last post by CPRES

Pharmacology and Pharmaceutics

  • 77 topics
  • 143 replies
Ebola Virus - last post by Einfolge

Histology and Pathology

  • 133 topics
  • 181 replies
First Nissl stain, then Gol... - last post by Marian3001


  • 32 topics
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how to make Percoll solutio... - last post by mdfenko


  • 57 topics
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5 M NaCl stock - last post by Ahyi

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