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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,788 topics
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Problem loading DNA / agaro... - last post by phage434

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 597 topics
  • 1,129 replies
Two-proportion z test: is i... - last post by dalemcameron


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 473 topics
  • 989 replies
Non-radioactive DNA-PK acti... - last post by mdfenko

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 121 topics
  • 317 replies
Are you a scientist/invento... - last post by jobscallumco

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,174 topics
  • 3,786 replies
Detection of Td-Tomato prot... - last post by Rsm

Tissue and Cell Culture

  • 1,672 topics
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Transducing HEK293 cells wi... - last post by ccsusx

Stem Cell

  • 135 topics
  • 201 replies
Stem Cell Culture Theory - last post by bob1

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 393 topics
  • 823 replies
Basic questions - last post by madelingirly

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,064 topics
  • 12,785 replies
TE buffer pH adjustment - last post by phage434

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,858 topics
  • 6,182 replies
problem in sequencing of gene - last post by phage434

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,182 topics
  • 6,050 replies
bacterial RNA isolation fro... - last post by Md Jibran Alam

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 627 topics
  • 1,325 replies
Sperm microRNA isolation - last post by RNA woman

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,037 topics
  • 3,971 replies
How to optimise my western... - last post by Podge

Protein Expression and Purification

  • 734 topics
  • 1,808 replies
Question about MBP purifica... - last post by skepticalguy99

SDS-PAGE and Western Blotting

  • 1,108 topics
  • 3,751 replies
band obove the ladder - last post by mdfenko


Discussions on antibody, immunohistochemistry and more...

  • 1,622 topics
  • 2,660 replies
Good ovalbumin antibody - last post by Ahrenhase


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,015 topics
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"luxury" microbiolo... - last post by phage434

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 197 topics
  • 324 replies
Genome annotation - last post by Dav

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 602 topics
  • 1,607 replies
housekeeping/reference gene... - last post by Lj Lee

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 324 topics
  • 956 replies
Amplicons detected for nega... - last post by trungbiotech

Macroarray and Microarray

Topics related to different array techniques

  • 122 topics
  • 220 replies
Microarray-Gene fold change... - last post by bob1


Questions related to neuroscience and other neuro-related fields

  • 185 topics
  • 260 replies
Neurophysiology- NERVE onli... - last post by TheForumLord

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 179 topics
  • 411 replies
female BALB/c mice eating a... - last post by VarianWrynn

Botany and Plant Biology

Topics specific to plants.

  • 106 topics
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Is it possible to stored en... - last post by hobglobin

Pharmacology and Pharmaceutics

  • 77 topics
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Ebola Virus - last post by Einfolge

Histology and Pathology

  • 129 topics
  • 177 replies
Protocole Immunohistochemis... - last post by orianedl


  • 32 topics
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how to make Percoll solutio... - last post by mdfenko


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5 M NaCl stock - last post by Ahyi

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