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Protocols and Techniques Forums


General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

  • 1,988 topics
  • 6,199 replies
Uses of gloves in a laboratory - last post by lucast

Bioinformatics and Biostatistics

Dealing mainly with bioinformatics applications such as sequence analysis, database search, primer design, and related software...

  • 663 topics
  • 1,212 replies
[Video] Using NCBI for RT-P... - last post by arine23


Technical questions related to chemical aspects of biomolecules such as carbohydrates, proteins, enzymes, lipids and nucleic acids.

  • 527 topics
  • 1,126 replies
What is Inulinase Enzyme? - last post by Victoria Goshal

Research Idea, Design and Collaboration

Need research ideas, strategies and collaborations?

  • 144 topics
  • 362 replies
New generation "vaccine... - last post by henrilentonen

Cell Biology

Questions on cell and tissue culture, cytometry ...

  • 2,254 topics
  • 3,880 replies
remove GH from FBS - last post by wuxx0153

Tissue and Cell Culture

  • 1,834 topics
  • 4,024 replies
Unidentified geometric obje... - last post by bob1

Stem Cell

  • 139 topics
  • 211 replies
Cryopreserving human ES dir... - last post by neuropath

Flow Cytometry

All about FACS such as cell preparation, staining, machine setting and operation, data analysis...

  • 433 topics
  • 865 replies
Fluorescence & Flow Cyt... - last post by queenc

Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

  • 6,291 topics
  • 13,197 replies
How to calculate rooted bif... - last post by Kelton

Molecular Cloning

Any techniques related to molecular cloning including restriction digestion, ligation, transformation, plasmid...

  • 1,986 topics
  • 6,537 replies
low copy number plasmid pur... - last post by Curtis

PCR, RT-PCR and Real-Time PCR

Questions related to any PCR, primers etc.

  • 2,368 topics
  • 6,466 replies
RNA degradation from cells... - last post by OldCloner

siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

  • 666 topics
  • 1,388 replies
miRNA isolation from serum - last post by seomelon06

Protein and Proteomics

Discussions on the isolation, identification, evaluation, and quantification of the level of exp​ression of proteins

  • 2,134 topics
  • 4,179 replies
blood contamination - last post by Zagami Francesco

Protein Expression and Purification

  • 827 topics
  • 2,049 replies
Protein expression issue - last post by hops proteon

SDS-PAGE and Western Blotting

  • 1,210 topics
  • 4,062 replies
Question on western blot pi... - last post by pito


Discussions on antibody, immunohistochemistry and more...

  • 1,685 topics
  • 2,773 replies
Ways to assess CD4+ T cell... - last post by harold91


Dealing with microscopic forms of life, including protozoans, algae, fungi, bacteria, viruses, etc.

  • 1,119 topics
  • 3,247 replies
No growth - last post by Phil Geis

Genetics and Genomics

Discussion on methods related to the study of heredity, variation, the genomes...

  • 229 topics
  • 349 replies
Webinar: ChIP-Seq and ATAC-... - last post by Partek

DNA Methylation and Epigenetics

Topics related to epigenetic study such as analysis of DNA methylation (bisulfite modification, MSP, BSP, CpG array, etc), histone modification (ChIP etc), chromatin structure...

  • 624 topics
  • 1,667 replies
"Binomial" and its... - last post by MindlessJPEG

ChIP and Next Generation Sequencing

Chromatin immunoprecipitation assays and related techniques such as RNA-ChIP, ChIP-on-Chip, ChIP sequencing

  • 349 topics
  • 989 replies
Variable ChiP-qPCR even in... - last post by Sandeep Gupta

Macroarray and Microarray

Topics related to different array techniques

  • 129 topics
  • 234 replies
online microarray data - last post by LizRepositive


Questions related to neuroscience and other neuro-related fields

  • 194 topics
  • 269 replies
How do photoreceptors disti... - last post by Trof

Animal and Zoology

Discussion on techniques involved in zoology as well as other disciplines which use animals as models

  • 185 topics
  • 419 replies
Is it possible to cause mor... - last post by molbiolnew

Botany and Plant Biology

Topics specific to plants.

  • 119 topics
  • 283 replies
How do I use Gibbrellic Acid - last post by MagicalPotato

Pharmacology and Pharmaceutics

  • 84 topics
  • 164 replies
Crosslinking with glutarald... - last post by labtastic

Histology and Pathology

  • 169 topics
  • 215 replies
Literature Review Suggestions - last post by dawudhilli


  • 33 topics
  • 83 replies
Freezing PBMCs that had bee... - last post by Trof


  • 65 topics
  • 169 replies
Water Treatment Process (la... - last post by Rahul sharma123

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