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General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

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Electrophoresis

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443 replies

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Topic	Stats	Last Post Info
Best Way To Prepare Sodium Bicarbonate Solution Started by SunsetSatine, 12 Dec 2012 Sodium Bicarbonate	1 reply 287 views	pito 12 Dec 2012
measurement of proteins in a mixture of soluble-insoluble proteins Started by marsiles, 18 Sep 2012	5 replies 284 views	mdfenko 19 Sep 2012
Phenol ChCl3--upper or lower phase (?) Started by srpres, 22 Oct 2012	5 replies 283 views	Ahrenhase 07 Nov 2012
Cell plating Started by vjlj, 18 Sep 2012	2 replies 281 views	vjlj 18 Sep 2012
Can i use spectrophotometer to replace microplate reader Started by hianghao, 11 Dec 2012	3 replies 281 views	hianghao 12 Dec 2012
Competitive vs. Sandwich ELISA Started by Ventilator, 21 Sep 2012	6 replies 278 views	Sotirios 25 Sep 2012
NBT solution in X-Gal staining Started by kkbarratt, 08 Nov 2012 NBT, cell culture, staining	1 reply 277 views	bob1 08 Nov 2012
help Started by pipo88, 11 Feb 2013	6 replies 277 views	mdfenko 13 Feb 2013
Plasmid Miniprep Started by Epigeneticist, 19 Nov 2012 Plasmid Miniprep	2 replies 273 views	Trof 20 Nov 2012
Whatman FTA cards Started by Marti, 17 Jan 2013 FTA cards, PCR	1 reply 272 views	mdfenko 20 Jan 2013
Low yield PCR product after gel purification Started by Surachet Benjathummarak, 13 Mar 2013	7 replies 271 views	2xzwei 20 Mar 2013
Making an acetate buffer Started by asta6801, 26 Feb 2013 buffer, chemistry, pH, pKa	4 replies 267 views	neuron 05 Mar 2013
BrdU immunofluorescence protocol Started by criscastells, 02 Nov 2012 BrdU	4 replies 266 views	criscastells 14 Nov 2012
Trouble shoot with radioactive Enzyme activity assay Started by mimiW, 18 Jan 2013	6 replies 264 views	neuron 23 Jan 2013
Proteinase K digestion time. Started by Wek, 27 Oct 2012	5 replies 263 views	prabhubct 28 Oct 2012
protein induction from yeast Started by anwesha1, 25 Sep 2012	1 reply 257 views	ascacioc 25 Sep 2012
Adjusting pH of RPMI/ bicarbonate containing media Started by RossM, 05 Nov 2012	9 replies 256 views	El Crazy Xabi 07 Nov 2012
Sodium Phosphate Buffer Started by R3search3R, 15 Nov 2012	4 replies 256 views	mdfenko 16 Nov 2012
Non-reproducible results/experiments Started by science noob, 14 Mar 2013	9 replies 255 views	mdfenko 15 Mar 2013
Packaging vectors Started by Apple1984, 26 Jan 2013	1 reply 254 views	zxs 04 Mar 2013
RFLP Problem Started by sidra_younis, 01 Jan 2013	2 replies 252 views	phage434 01 Jan 2013
solutions containing different concentrations of oxaloacetate, malate, and NADH Started by ladwer, 06 Oct 2012	1 reply 251 views	bob1 10 Oct 2012
questions about centrifugation and organelle lysis Started by keegi, 28 Sep 2012	1 reply 248 views	bob1 28 Sep 2012
Ferum level estimation in saliva Started by Matiw91, 09 Oct 2012	6 replies 248 views	mdfenko 10 Oct 2012
Oil red O preparation Started by julnobody, 26 Jan 2013	1 reply 246 views	hobglobin 28 Jan 2013