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General Lab Techniques

Lab basic skills such as centrifugation, spectrophotometry, quantification, etc...

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Electrophoresis

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Topic	Stats	Last Post Info
Answered Calculation Started by Mad Researcher, 10 Jan 2018 calculation, antibody, dilution	7 replies 1,259 views	bob1 12 Jan 2018
Answered Bioluminescence vs Fluorescence Microscope Started by Thomson, 13 Dec 2017 bioluminescence, fluorescence and 1 more...	5 replies 704 views	bob1 21 Dec 2017
Answered Ratio to ?g/?l Started by Mad Researcher, 02 Dec 2017 calculation, antibody calculation and 2 more...	1 reply 953 views	mdfenko 04 Dec 2017
Answered Gel Electrophoresis: Gel Poured With Water Started by djvan, 29 Jul 2016	2 replies 777 views	djvan 30 Jul 2016
Answered Stock solution Started by Newbs, 22 Feb 2016 stock solution, dilution	4 replies 889 views	El Crazy Xabi 23 Feb 2016
Answered Serial dilution Started by Mad Researcher, 18 Nov 2015 serial dilution	6 replies 1,100 views	mdfenko 20 Nov 2015
Answered Alternatives to ice when working with samples. Started by Dr. N00b, 14 Jul 2015 lab ice bucket, working with ice 1 2	Hot 15 replies 1,450 views	labtastic 29 Jul 2015
Answered Type B cycle Autoclave - Liquids? Started by Dr. N00b, 14 Jun 2015 Type B cycle, autoclave, liquids	8 replies 1,497 views	El Crazy Xabi 21 Jun 2015
Answered Lack of reproducibility using BCA to quantitate total protein Started by Michael Starr, 10 Mar 2015 BCA, contamination	5 replies 999 views	Michael Starr 24 Apr 2015
Answered How many people pellet bacteria cultures in their tissue culture centrifuge? Started by 5280, 24 Jan 2015 Tissue culture, Bacteria culture and 2 more...	4 replies 1,182 views	CPRES 27 Jan 2015
Answered To prepare 1M calcium chloride Started by Meg P. Anula, 12 Jan 2015 calcium chloride, competent cells	3 replies 1,064 views	CPRES 13 Jan 2015
Answered Dilute to a working concentration Started by dendrite, 06 Oct 2014 dilution	1 reply 957 views	mdfenko 06 Oct 2014
Answered DNA stability in STE with proteinase K Started by SG181, 18 Jun 2014	1 reply 697 views	Trof 18 Jun 2014
Answered Conversion from mw or MU to mg/ml help Started by Jones1233211, 01 May 2014	9 replies 683 views	Jones1233211 05 May 2014
Answered Possible to sort cells from formalin? Started by Pazit, 23 Feb 2014	1 reply 866 views	Rsm 27 Feb 2014
Answered Is it generally acceptable to ask another lab for cell line/plasmid/etc Started by Ahrenhase, 09 Feb 2014	4 replies 1,065 views	bob1 24 Feb 2014
Answered Can somebody explain to me what "spiking" means in RT-PCR and why do you Started by That_Lab_Guy, 18 Nov 2013 RT-PCR, Spiking, Positive control	3 replies 2,271 views	mdfenko 30 Dec 2013
Answered Making a stock solution for my drug using DMSO Started by Frazermb, 15 Oct 2013 cAMP, cGMP, DMSO and 2 more... 1 2	Hot 16 replies 2,213 views	Frazermb 16 Oct 2013
Answered Is there a buffer at acidic pH? (pH 1 and 2) Started by dustbunny000, 04 Sep 2013 buffers, acidic pH, pH 1, pH 2 1 2	Hot 22 replies 1,903 views	mdfenko 08 Oct 2013
Answered Diffuse rRNA bands on RNA denaturing (formaldehyde) gel Started by RNAfreak, 28 Aug 2013 RNA denaturing gel and 2 more...	5 replies 1,072 views	RNAfreak 07 Sep 2013
Answered How to propagate Electro-competent DB3.1 cells Started by Trys, 17 Aug 2013	6 replies 799 views	Trys 18 Aug 2013
Answered Difference between PFA, acetone and methanol in cell fixation Started by science noob, 12 Aug 2013	1 reply 1,026 views	El Crazy Xabi 13 Aug 2013
Answered Cost of Sequencing by Post Started by centralamgod, 02 Aug 2013	8 replies 1,061 views	BMF 05 Aug 2013
Answered Calculation of a dilution factor?? Started by esbena, 01 Aug 2013 dilution, serum, factor, HPLC	2 replies 3,057 views	esbena 01 Aug 2013