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siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

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RNAi Interest Group

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Topic	Stats	Last Post Info
Different amount of oligonucleotide Started by dulat, 27 Sep 2012 transfection, cell culture, RNAi	1 reply 278 views	bob1 27 Sep 2012
miRZIP technology Started by Shifting Reality, 03 May 2012	0 replies 274 views	Shifting Reality 03 May 2012
What is the best way to overexpress miRNAs? Started by leochicaybam, 17 Oct 2012	1 reply 274 views	pcrman 01 Nov 2012
[Question] primer sequence for zebrafish U6 in SYBR-qPCR for miR normalization? Started by lydialing, 19 Oct 2012 miR, zebrafish, qPCR, U6	0 replies 273 views	lydialing 19 Oct 2012
Genotype RNAi line Started by ladyjane, 21 Sep 2010	0 replies 271 views	ladyjane 21 Sep 2010
Anyone used pHrodo? Started by Napra, 30 Sep 2010	0 replies 269 views	Napra 30 Sep 2010
Dual Luciferase Assay Started by elray152, 05 Apr 2013	0 replies 268 views	elray152 05 Apr 2013
Can I use custom made synthetic miRNA for Overexpression? Started by bar2012, 30 Jul 2012 Custom miRNA	1 reply 267 views	pcrman 30 Jul 2012
Does anyone have experience with Vector Based antagomir ? Started by theofear, 25 Apr 2011	0 replies 266 views	theofear 25 Apr 2011
Knockdown induce apoptosis Started by nkjeriksson, 09 Jul 2012	5 replies 262 views	Curtis 18 Jul 2012
How do different cell types respond to RNAi? Started by APO34tex, 09 May 2012	0 replies 260 views	APO34tex 09 May 2012
Synthetic microrna for normalization in serum samples Started by zammybutt, 25 Oct 2012 serum microrna, normalization and 2 more...	1 reply 258 views	Janne 29 Oct 2012
Help! No knockdown if EGFP with HEK 293/GFP cells from Cell Biolabs Started by eesem, 25 Jun 2012	0 replies 257 views	eesem 25 Jun 2012
much smaller fragments after IVT Started by mahrak, 30 Dec 2012	0 replies 254 views	mahrak 30 Dec 2012
siRNA phRodo conjugation Started by Napra, 30 Sep 2010	0 replies 243 views	Napra 30 Sep 2010
To small amount of cells used for shRNA transfection Started by Amer Mahmood, 15 Apr 2013 shRNA, transfection and 1 more...	1 reply 243 views	bob1 15 Apr 2013
Hiperfect siRNA in HT-29 cells help Started by Elioelio, 16 Oct 2012 siRNA, HT-29, HT29, Hiperfect	2 replies 241 views	Elioelio 18 Oct 2012
pTRIPZ system from openbiosystems Started by Miron, 12 Aug 2012 pTRIPZ;, Lentivirus; and 3 more...	0 replies 238 views	Miron 12 Aug 2012
siRNA in E.Coli Started by Pangea, 04 Jan 2013 siRna ecoli	2 replies 232 views	Pangea 05 Jan 2013
siRNA based on published shRNA Started by doxorubicin, 06 Jun 2012 sirna design	0 replies 231 views	doxorubicin 06 Jun 2012
Regulation of endoderm formation and left-right asymmetry by miR-92 during early Started by Jon Moulton, 20 Apr 2011	0 replies 230 views	Jon Moulton 20 Apr 2011
Little knockdown but big effect on proliferation Started by Alsulaiman, 24 Jul 2012	2 replies 229 views	zodiac1505 27 Jul 2012
SuperScript II RT instead of TaqMan® MicroRNA Reverse Transcription Kit Started by smartyspb, 20 Mar 2013 miRNA, cDNA and 2 more...	0 replies 228 views	smartyspb 20 Mar 2013
Not RNAi. Has anyone tried gene knock-up? Started by mhj222, 11 Feb 2013	2 replies 225 views	bob1 11 Feb 2013
shRNA designed for AB1 gene also knockdown AB2 Started by Yen-Ling Chiu, 08 Nov 2012	2 replies 222 views	Jon Moulton 09 Nov 2012