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siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

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RNAi Interest Group

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Topic	Stats	Last Post Info
Pinned olligo annealing for shRNA cloning Started by yean_ny_nie, 25 May 2009 1 2	Hot 20 replies 14,582 views	iamdhan 05 Aug 2010
Pinned Different siRNA transfection agents Started by caro, 16 Dec 2004	13 replies 10,414 views	EEO 14 Feb 2011
Pinned pre-miRNA existence Started by j123, 26 Jan 2010	13 replies 6,212 views	Fizban 07 Jun 2010
Pinned Different ways of doing RNAi Started by green, 27 Jan 2004	10 replies 8,415 views	Andras 31 Aug 2011
Pinned shRNAs work in vitro, not in vivo Started by ambv34, 27 Jul 2010	10 replies 3,698 views	canine 07 Aug 2011
Pinned siRNA target selection problem Started by valine, 20 Jan 2003	8 replies 7,814 views	DNA_modified_cabbage 27 Jul 2011
Pinned RNAi problem - Gene is knocked down, but not protein Started by Jean Lu, 29 Sep 2011 shRNA, protein, RNA, rnai, sirna and 1 more...	8 replies 1,772 views	mhj222 11 Feb 2013
Pinned Nuclear RNA knockdown with siRNA Started by Nick, 09 Feb 2005	5 replies 6,048 views	ribonerd 18 Nov 2010
Pinned stable transfection with siRNA expression vector Started by boduolige, 03 May 2004	4 replies 7,118 views	Cookies 14 Mar 2005
Pinned Promoters and vectors for shRNA? The need for specific vectors and promoters? Started by lab_member, 14 Jun 2009	4 replies 4,886 views	lab_member 16 Jun 2009
Pinned How to retrieve precursor miRNA sequence with extra bases for cloning Started by newmirnaman, 23 Apr 2010	4 replies 3,087 views	gingerblack 07 Dec 2011
Pinned Old threads on "siRNA, microRNA and RNAi" are achived here Started by bioforum, 03 Feb 2009	3 replies 5,113 views	Jon Moulton 15 Feb 2012
miRNA isolation from liquid samples Started by FACS_flow, 21 Jul 2009 1 2	Hot 18 replies 7,593 views	SAN1017 23 Jul 2010
To Fizban Started by newmirnaman, 29 Mar 2009	13 replies 4,433 views	findik44 04 Jun 2012
Define Trasnsfection efficiency interms of siRNA experiment Started by rajgene, 16 Feb 2009	12 replies 5,728 views	rajgene 18 Feb 2009
miRNA isolation from serum Started by mulain, 17 Mar 2010	12 replies 5,589 views	Sero 22 Dec 2010
total RNA (for miRNA) extraction from human serum Started by mulain, 05 Jan 2010	11 replies 4,068 views	Biodate 12 Mar 2010
Lentivirus-based Knockdown for miRNAs? Started by deepgreenbegum, 02 Nov 2010	10 replies 1,758 views	Earnest 24 Mar 2011
Is overexpression a correct way to check shRNA knockdown efficiency? Started by gyma, 27 Dec 2012 overexpression, shRNA and 1 more...	9 replies 432 views	bob1 05 Jan 2013
Probe size for miRNA Northern blot? Started by Irina, 23 Apr 2009	8 replies 3,685 views	Irina 01 May 2009
shRNA stable line Started by Dr Teeth, 05 Nov 2009	8 replies 3,585 views	skeuos 06 Nov 2009
Lentivial Knockdown of miRNAs Started by Samantha, 07 Jan 2010	8 replies 2,540 views	redhorse 02 Apr 2010
The reliable data of microRNA expression from SYBR-stem loop PCR or ABI Taqman m Started by ljchen, 16 Jan 2010	8 replies 2,384 views	Fizban 15 Dec 2011
Help! ES Lenti infection efficiency=nightmare Started by Radish, 25 Jan 2011	8 replies 1,144 views	Radish 01 Feb 2011
Help with real-time PCR quantification of miRNA Started by MicroRNAQa, 31 Jan 2011	8 replies 1,670 views	UBClabbie 16 Mar 2011