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siRNA, microRNA and RNAi

Topics on RNAi and small molecular RNAs such as siRNA, microRNA.

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RNAi Interest Group

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Topic	Stats	Last Post Info
RNA journals Started by mahrak, 05 May 2013	4 replies 162 views	mahrak 08 May 2013
shRNA Lentiviral Transduction- loss of knockdown after 1-3 passages Started by Tensix, 30 Apr 2013 shRNA, lentivirus	1 reply 155 views	pcrman 07 May 2013
To small amount of cells used for shRNA transfection Started by Amer Mahmood, 15 Apr 2013 shRNA, transfection and 1 more...	1 reply 261 views	bob1 15 Apr 2013
shRNA interference using Lenti virus vector Started by Mohamed samir, 07 Apr 2013	1 reply 367 views	bob1 08 Apr 2013
miRNA quantification Started by dude3005, 18 Mar 2013 qPCR, reverse transcription and 1 more...	3 replies 354 views	imoani 26 Mar 2013
SuperScript II RT instead of TaqMan® MicroRNA Reverse Transcription Kit Started by smartyspb, 20 Mar 2013 miRNA, cDNA and 2 more...	0 replies 250 views	smartyspb 20 Mar 2013
lentiviral packaging - what is the best ratio of packaging vectors Started by zxs, 04 Mar 2013 lentiviral, packaging, vector	0 replies 222 views	zxs 04 Mar 2013
miRNA expression profiling Started by SriniEHE, 07 Dec 2012 miRNA	3 replies 652 views	balintblaszlo 03 Mar 2013
Not RNAi. Has anyone tried gene knock-up? Started by mhj222, 11 Feb 2013	2 replies 236 views	bob1 11 Feb 2013
Gene knockdown expression levels shRNAmirs Started by marspimp, 17 Jan 2013 Gene knockdown	2 replies 325 views	marspimp 29 Jan 2013
in vivo naked-mRNA delivery Started by mahrak, 26 Nov 2012 mRNA delivery	4 replies 437 views	mahrak 12 Jan 2013
Is overexpression a correct way to check shRNA knockdown efficiency? Started by gyma, 27 Dec 2012 overexpression, shRNA and 1 more...	9 replies 455 views	bob1 05 Jan 2013
siRNA in E.Coli Started by Pangea, 04 Jan 2013 siRna ecoli	2 replies 242 views	Pangea 05 Jan 2013
much smaller fragments after IVT Started by mahrak, 30 Dec 2012	0 replies 259 views	mahrak 30 Dec 2012
siRNA resuspension in RNase-free water Started by Neanderthal, 27 Dec 2012 siRNA resuspension and 3 more...	1 reply 296 views	bob1 28 Dec 2012
Recommendation on siRNA transfection reagents Started by Brad Ruan, 09 Nov 2012	2 replies 377 views	Neanderthal 27 Dec 2012
Mechanism of effector strand selection on cellular sRNA Started by Rishi Aryal, 15 Dec 2012 miRNA, siRNA, small RNA and 2 more...	0 replies 382 views	Rishi Aryal 15 Dec 2012
Annealed oligos vs. non annealed. Electrophoresis pattern. Started by Raquelina, 10 Dec 2012 oligo annealing shrna	4 replies 336 views	Raquelina 11 Dec 2012
microRNA promoter based expression vector? Started by assembler01, 07 Dec 2012 microRNA, expression	1 reply 356 views	pcrman 07 Dec 2012
microRNA validation in vivo Started by alia, 01 Dec 2012	1 reply 286 views	pcrman 02 Dec 2012
SiLAC and SilAD methods Started by alia, 01 Dec 2012	0 replies 210 views	alia 01 Dec 2012
shRNA with 100% marker expression but no knockdown efficiency at all Started by gyma, 21 Nov 2012 shRNA, sorting	7 replies 524 views	gyma 29 Nov 2012
shRNA designed for AB1 gene also knockdown AB2 Started by Yen-Ling Chiu, 08 Nov 2012	2 replies 229 views	Jon Moulton 09 Nov 2012
How long is the suppressive efficiency by siRNA or shRNA Started by Nicole Wa, 02 Nov 2012	2 replies 299 views	Nicole Wa 02 Nov 2012
What is the best way to overexpress miRNAs? Started by leochicaybam, 17 Oct 2012	1 reply 286 views	pcrman 01 Nov 2012