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DNA Methylation and Epigenetics

Please also visit important archived forum threads of the DNA Methylation and Epigenetics Forum and on the topics of bisulfite treatment, BSP, MSP, DNA methylation, CpG island, and gene promoter.

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Topic	Stats	Last Post Info
Help! No bands after BSP! Started by YYZDNA, 08 Aug 2012	2 replies 383 views	vilperte 08 Feb 2013
Mysterious peaks in pyrosequencing runs!? Started by methylmouse, 06 Aug 2012 pyrosequencing	2 replies 292 views	methylmouse 13 Aug 2012
Problems with MS-HRM Standard Curve! Started by Bianabi, 02 Aug 2012	0 replies 175 views	Bianabi 02 Aug 2012
5-hmC and 5-mC Analysis with Control DNA of the Kit Started by yasemin, 31 Jul 2012 DNA methylation and 2 more...	0 replies 201 views	yasemin 31 Jul 2012
Can we use degenerate bases in BSP primers? Started by paulcross, 25 Jul 2012 degenerate bases, BSP, primer and 1 more...	1 reply 352 views	pcrman 26 Jul 2012
Plasmid clone in BSP Started by henry_cheng, 16 Jul 2012 DNA methylation	1 reply 259 views	pcrman 20 Jul 2012
Sequencing of the PCR production in BSP Started by henry_cheng, 16 Jul 2012 DNA methylation	1 reply 380 views	pcrman 16 Jul 2012
Taq polymerase for MSP Started by jiajiatao, 12 Jul 2012 taq, msp	1 reply 370 views	pcrman 13 Jul 2012
Spin columns with bisulfite conversion Started by ForEpi, 06 Jun 2012 Bisulfite conversio	1 reply 329 views	lmg 07 Jun 2012
Which beads should I use with IgM antibodies for ChIP? Started by biznatch, 05 Jun 2012 IgM, protein L, magnetic beads	0 replies 304 views	biznatch 05 Jun 2012
What 260/280 and 260/230 ratio should I expect from sonicated chromatin? Started by biznatch, 28 May 2012 chromatin, chip, nanodrop and 2 more...	0 replies 382 views	biznatch 28 May 2012
Microarray 244K- what does it mean Started by rose1984, 20 May 2012	1 reply 289 views	Paulgs3 21 May 2012
Problem of Direct bisulfite sequencing Started by ruiiris, 16 May 2012 Bisulfite, direct sequencing	1 reply 463 views	biznatch 28 May 2012
Bisulfite cloning... reading material Started by rose1984, 12 May 2012	1 reply 365 views	biznatch 28 May 2012
DNA in methylation analysis Started by egruano, 27 Apr 2012 DNA isolation metho	3 replies 577 views	NXT-Dx 22 May 2012
sonication of Discs and CNS (formaldehyde crosslinked) from Drosophila 3rd Insta Started by des22, 26 Apr 2012	0 replies 271 views	des22 26 Apr 2012
chip seq protocol Started by seeking_2012, 26 Apr 2012	5 replies 639 views	ivap 29 Aug 2012
MSP Primers not working Started by FolateGirl, 20 Apr 2012 Methylation, PCR Primers	6 replies 758 views	katjaze 10 Jun 2012
dna sonication for viscosity measurement Started by rima, 17 Apr 2012 sonication, white particles	0 replies 342 views	rima 17 Apr 2012
How to strore nucleosome for the in invtro assay Started by guyan, 04 Apr 2012	0 replies 350 views	guyan 04 Apr 2012
2D of histones Started by pakbiochemist, 06 Mar 2012	0 replies 367 views	pakbiochemist 06 Mar 2012
EPIGENETICS-NEWBIE-Weird/too close primers for pyrosequencing assay Started by ursa, 29 Feb 2012 pyro, primers, mistmatch	2 replies 644 views	methylnick 29 Mar 2012
plotting DNA methylation profile vs TSS Started by honey, 22 Feb 2012 methylation	1 reply 501 views	methylnick 29 Mar 2012
Methylation sensitive enzymes Started by CpGIsland, 16 Feb 2012	1 reply 535 views	methylnick 29 Mar 2012
problems with Bisulfite PCR Started by vaidhi, 12 Feb 2012	1 reply 701 views	methylnick 29 Mar 2012