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DNA Methylation and Epigenetics

Please also visit important archived forum threads of the DNA Methylation and Epigenetics Forum and on the topics of bisulfite treatment, BSP, MSP, DNA methylation, CpG island, and gene promoter.

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Topic	Stats	Last Post Info
how to find promoter sequence- my blat search doesnt seem to work? Started by shelleyanne1983, 12 Sep 2012	3 replies 283 views	timeman 15 Sep 2012
designing methylation specific primers Started by shelleyanne1983, 11 Sep 2012	3 replies 343 views	lmg 11 Sep 2012
"coding strand" or " template strand" ? Started by paulcross, 02 Sep 2012	13 replies 488 views	paulcross 04 Sep 2012
chip seq protocol Started by seeking_2012, 26 Apr 2012	5 replies 644 views	ivap 29 Aug 2012
ChiP assay band in negative control Started by ivap, 29 Aug 2012 Chip, Mouse IgG PCR	0 replies 271 views	ivap 29 Aug 2012
Different primer optimalization for nested vs direct MSP? Started by methylmouse, 13 Aug 2012 methylation, MSP, primer design	3 replies 351 views	pcrman 16 Aug 2012
Mysterious peaks in pyrosequencing runs!? Started by methylmouse, 06 Aug 2012 pyrosequencing	2 replies 292 views	methylmouse 13 Aug 2012
Problems with MS-HRM Standard Curve! Started by Bianabi, 02 Aug 2012	0 replies 176 views	Bianabi 02 Aug 2012
5-hmC and 5-mC Analysis with Control DNA of the Kit Started by yasemin, 31 Jul 2012 DNA methylation and 2 more...	0 replies 201 views	yasemin 31 Jul 2012
Can we use degenerate bases in BSP primers? Started by paulcross, 25 Jul 2012 degenerate bases, BSP, primer and 1 more...	1 reply 354 views	pcrman 26 Jul 2012
Plasmid clone in BSP Started by henry_cheng, 16 Jul 2012 DNA methylation	1 reply 262 views	pcrman 20 Jul 2012
Sequencing of the PCR production in BSP Started by henry_cheng, 16 Jul 2012 DNA methylation	1 reply 382 views	pcrman 16 Jul 2012
Taq polymerase for MSP Started by jiajiatao, 12 Jul 2012 taq, msp	1 reply 373 views	pcrman 13 Jul 2012
MSP Primers not working Started by FolateGirl, 20 Apr 2012 Methylation, PCR Primers	6 replies 760 views	katjaze 10 Jun 2012
MSP Started by InduS, 29 Mar 2011	10 replies 2,251 views	katjaze 10 Jun 2012
Spin columns with bisulfite conversion Started by ForEpi, 06 Jun 2012 Bisulfite conversio	1 reply 331 views	lmg 07 Jun 2012
Which beads should I use with IgM antibodies for ChIP? Started by biznatch, 05 Jun 2012 IgM, protein L, magnetic beads	0 replies 308 views	biznatch 05 Jun 2012
Bisulfite cloning... reading material Started by rose1984, 12 May 2012	1 reply 367 views	biznatch 28 May 2012
Problem of Direct bisulfite sequencing Started by ruiiris, 16 May 2012 Bisulfite, direct sequencing	1 reply 464 views	biznatch 28 May 2012
What 260/280 and 260/230 ratio should I expect from sonicated chromatin? Started by biznatch, 28 May 2012 chromatin, chip, nanodrop and 2 more...	0 replies 384 views	biznatch 28 May 2012
High throughput methylation mapping - Infinium or other? Started by lmg, 30 May 2011	3 replies 759 views	methylnick 24 May 2012
DNA Enrichment for Methylation Analysis Started by Muhammad Umer, 25 Aug 2009	8 replies 5,860 views	NXT-Dx 22 May 2012
DNA in methylation analysis Started by egruano, 27 Apr 2012 DNA isolation metho	3 replies 578 views	NXT-Dx 22 May 2012
Microarray 244K- what does it mean Started by rose1984, 20 May 2012	1 reply 291 views	Paulgs3 21 May 2012
Inter assay variation in global methylation Elisa Started by Taqman, 15 Feb 2011	2 replies 952 views	Sapitron 14 May 2012