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Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

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Topic	Stats	Last Post Info
Restriction Enzymes Started by Pangea, 07 Jan 2013 Re Cloning	4 replies 218 views	pito 08 Jan 2013
Can I use Real-time PCR instead of Southern Blot to determine number of integran Started by Nyhna, 23 Jan 2013 Southern Blot, Real time PCR, DIG	1 reply 218 views	bob1 24 Jan 2013
RNeasy kit + DNAse digest from Promega Started by Podge, 23 Oct 2012	0 replies 216 views	Podge 23 Oct 2012
Neomycin resistance sequence Started by weswes, 24 Jan 2013 Neomycin resistance	1 reply 216 views	cellthetruth 24 Jan 2013
DNA EtOH/Sodium Acetate precip over the weekend...still good? Started by Denny, 17 Nov 2012	2 replies 215 views	Denny 17 Nov 2012
Atypical yeild of RNA Started by atashia, 19 Nov 2012	1 reply 215 views	Trof 19 Nov 2012
sacB Counter-Selection to generate double cross-over deletion mutants Started by gandhi, 20 Mar 2013 sacB, Allelic Exchange and 3 more...	3 replies 214 views	gandhi 10 Apr 2013
Creating primers to add restriction sites to vector backbone Started by LacquerHead, 11 Apr 2013	7 replies 214 views	phage434 12 Apr 2013
Cytoplasmic mRNA isolation protocol Started by Niwrad, 04 Dec 2012 cytoplasmic mRNA, protocol	1 reply 213 views	bob1 04 Dec 2012
Qiagen re using column Started by emadsadeghian, 07 Jan 2013 Qiagen, spin column	5 replies 213 views	neuron 08 Jan 2013
leaky promoter Started by uday, 21 Apr 2013 leaky promoter	1 reply 212 views	phage434 21 Apr 2013
methods to extract RNA from blood sample? Started by iamleos, 19 May 2013	4 replies 212 views	AquaPlasmid 31 May 2013
Primers to amplify Kan operon from pet28 Started by nishachara, 04 Jan 2013 pet28, kanamycin	2 replies 211 views	neuron 08 Jan 2013
Weak promoter activity in pGL3 reporter assay Started by srpres, 24 May 2013 promoter, pGL3, luciferase	5 replies 210 views	pcrman 13 Jun 2013
Continuous growth of E. coli on AMP LB plate Started by tihong10, 05 Feb 2013 e. coli, lawn, amp	2 replies 209 views	tihong10 25 Feb 2013
Blue fluorescent color of agarose gel in UV Started by neuron, 14 Feb 2013	7 replies 209 views	neuron 19 Feb 2013
quantifying the activity of a transcription factor Started by mihoz, 21 Oct 2012	3 replies 207 views	bob1 22 Oct 2012
contamination possibility Started by xxcici, 13 May 2013	5 replies 206 views	notjustalabtech 09 Jun 2013
Has anyone reused any BigDye Cleanup plates? Started by Lefty, 24 Jan 2013 BigDye, Sequencing, CleanUp and 1 more...	0 replies 205 views	Lefty 24 Jan 2013
Transfection of a non human gene into human cell line Started by sanjiun, 26 Dec 2012	1 reply 204 views	bob1 26 Dec 2012
Extraction of Ribosomes Started by centropagus, 06 Nov 2012	1 reply 201 views	Curtis 09 Nov 2012
CRE mediated recombination problems Started by Shrin, 31 Oct 2012 CRE mES Cells	0 replies 199 views	Shrin 31 Oct 2012
Placental RNA extraction with qiagen rneasy mini plus kit Started by sheon, 05 Mar 2013	9 replies 198 views	bob1 10 Mar 2013
His tag introduction in to gene and primer design Started by Always_student, 10 Mar 2013 His tag, primer design	2 replies 196 views	Always_student 15 Mar 2013
problems with protein expression (or transfection) from Gateway plasmids? Started by lna, 15 Apr 2013	0 replies 196 views	lna 15 Apr 2013