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Molecular Biology

Questions related to PCR, cloning, DNA, RNA ...

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Topic	Stats	Last Post Info
Creating primers to add restriction sites to vector backbone Started by LacquerHead, 11 Apr 2013	7 replies 202 views	phage434 12 Apr 2013
cDNA test on 1% agarose gel Started by xxcici, 11 Apr 2013	3 replies 119 views	bob1 11 Apr 2013
Plasmid isolation from gram positive bacteria Started by 2xzwei, 10 Apr 2013	2 replies 178 views	2xzwei 15 Apr 2013
Are two promoters needed for co-expressing 2 proteins from the same plasmid in S Started by donny, 08 Apr 2013	4 replies 345 views	donny 12 Apr 2013
rsaI enzyme Started by Lay La, 05 Apr 2013	1 reply 306 views	bob1 05 Apr 2013
How to check for the presence of plasmid? Started by mimiW, 05 Apr 2013	3 replies 280 views	2xzwei 10 Apr 2013
P1 transduction: What will happen if more than 2 FRT sequences exist Started by Qundo12, 03 Apr 2013 P1 transduction, E. coli and 2 more...	0 replies 220 views	Qundo12 03 Apr 2013
Extracting total RNA from woody plant roots Started by cinnamon wattle, 01 Apr 2013 RNA, shrub roots	2 replies 468 views	cinnamon wattle 04 Apr 2013
Quantify total DNA in small cell lysate...? Started by assembler01, 27 Mar 2013 quantify, DNA, lysate	1 reply 713 views	bigudukaz 28 Mar 2013
camelpox DNA extraction Started by nightingale, 27 Mar 2013 help	0 replies 164 views	nightingale 27 Mar 2013
Quantify DNA in gel electrophoresis bands Started by Sylvia Joanne, 26 Mar 2013 gel electrophoresis, quantify	7 replies 1,241 views	Sylvia Joanne 28 Mar 2013
Protein extraction and isolectric focusing Started by SailorNorway, 24 Mar 2013	1 reply 156 views	SailorNorway 24 Mar 2013
problem with gene knockout by homologous recombination: crossover always happen Started by Lynn Shay, 22 Mar 2013	0 replies 145 views	Lynn Shay 22 Mar 2013
getting strange data in Luciferase-renilla assay Started by gingerx, 21 Mar 2013 luciferase-renilla	2 replies 166 views	gingerx 25 Mar 2013
Homozygote DNA but Heterozygote RNA? Started by Norway, 21 Mar 2013	2 replies 121 views	Norway 21 Mar 2013
sacB Counter-Selection to generate double cross-over deletion mutants Started by gandhi, 20 Mar 2013 sacB, Allelic Exchange and 3 more...	3 replies 199 views	gandhi 10 Apr 2013
TA Cloning of alpha-1,3 glucanase: No blue colonies Started by sfora71, 19 Mar 2013	5 replies 281 views	bob1 09 Apr 2013
What is the necessary time for lipofectamin to make complex with pre-miRNA oligo Started by gingerx, 19 Mar 2013 transfection, miRNA	1 reply 170 views	bob1 20 Mar 2013
what is the ORFeome clone? Started by lna, 17 Mar 2013 ORF, cloning	2 replies 165 views	lna 18 Mar 2013
ligation with one ristrection site/enzyme Started by yusii, 16 Mar 2013 ristriction digest, ligation and 2 more...	3 replies 228 views	2xzwei 20 Mar 2013
No amplification during RT-PCR Started by rmbio, 16 Mar 2013 cDNA synthesis problem?	4 replies 149 views	Rajpoot 17 Mar 2013
rescue cloning of a transposon mutant Started by shawn6022, 15 Mar 2013 transposon mutagenesis and 1 more...	2 replies 124 views	shawn6022 17 Mar 2013
Dephosphorylation Started by lucilius, 15 Mar 2013	7 replies 158 views	lucilius 17 Mar 2013
study of receptors in cell lines Started by SF_HK, 15 Mar 2013	1 reply 138 views	GNANA 15 Mar 2013
Mouse genotyping issues Started by LacquerHead, 13 Mar 2013	1 reply 126 views	bob1 13 Mar 2013